pycom08g03750

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0019185
Physical Location & Seq
Forward (+)
2882775 .. 2885001
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1764 bp
ATGAATTTGCCAGATCAGTCTATGAACTTGCCAATCGGGCAAAAACAACTCGACAAGCCCGAATTGCTGTGGAAGTCATACAATGACTTTGAACTAGCAGAGAGAGAGTTTGAGGGAGCCAGAAATCTGTACGGGCGACTCTTAGAACGGACCCAACACGTTAAAGTCTGGATCAGTTACGCAGCATTCAAGGCATCGGCTATGGCGGGAGGAGACGGAATGCGCTCAGAAGATGTTATTGAGCAAAAGGAACAATGCATTCAGCGCGCCAGAAGGGTTTTCGAGGGAGCGTTGGACGATTTTAGAACATCAGCGAATACTAGAAATCCTCAACATCATGACTTGTATCTCCTACGGATTTCAAAGGAACGACAAGCCATTCAAGAAACACGAAAGAATATGTGCCATGTTTTGGAAGAACCTGAAGATGCTACTGAGGTTAAGTCCCTTGCTCAAACTCATACAGACCAGATTATGGAGAATCAGGCACATGTTACCATTGACATCGAACGGAAGGATGGAAAATGGGTGAAACTACTGAAACACTGCATCTACCGGTCCCCGTCCATTTCATTTCATGCAGTCAGTAGCTCACAGCAGAAATCATCGAATACTCCTCGCAAAGCCAACCACACACAAAAAAACCCAACAAACCCATTTCATAACGTTCCACTAAAATCAAGAAATCCATACACAACAAACACAATGCTTACCCAAAACGCTTACTTTCAACTAAAAATTTCCATCTGGGTACTTTCAGACGAACCCAAAAAACCATCCAAAAAATTACAATATAGAGCAGCACAATTAAAGGAGAAAGGCAGAGAATTTGGACCTTGGGGAGGTCAATATGCTCGTAAAGAGTCCAACCGGACGAACAATCATGATCCTCGCAGCTGTGGTCGTGCAGGCAAGCCATCTCTCTTTCCCTCTGTTACTGAATTTCTCACAAGAAAATTACGAAAGACGAAGGGAGAAGAAATAGCCAGAAATAAGAGCCGAGCCGAGCCGTCTTCATCTTTTTTTTGTTCCTTACTAAATGAAACAATAGTTCATCAACTAAAAATTCATTACCATTGGTTCCTCAAGTCATCAAAATGTGTAGATCTGGTCATTTTCGTCAACTTCATCTGGTCATTTTCGTCAATTTTGTTAGAATTTTATCAAAATGAGTTATGTTGGAAGGATCATTGTTGCAATTGGGGTCCCTCAACTCATCAAAACGTGTGGCTATGGTCATTTTCGTCACATTCGTCACATTTGTATCAAAATGAGCTATGTTGGAATGACCATTACTACAATTTAGTTAAAGTTAAAGGATCATTGCTCCAATTTGGAATTAATAATAATAAATTTTTAGTTACGAAACCATTGATGTTTAAAAAAAAACGTTACTACAATTTACCCTTACGAAATTGGCACAAAAGTAAAGGGCACAGAGGGGAACTCCTAGTCCAATTTTATCGCGGACTCAGTTTCCTGTTTGATTTGGGATTCTCGATTTCCTATTTGATTTGGGACACTCGGTCTCTTATATATTGTGATTTTCTTGGGGTTTTTTCGAATTCGAGTTCAAGCTTTTTAGTTTCTAAAGAATTATGGCGAAGGACAACAATGGCTACAAGGACACCGATGACAAGCTGCCGCGGGCGAGAAGGGTTAAGAACAAAGCCCCAGCCCCTGTCCAAATCACCGCAGAGCAACTCTTCCGCGAGGCGCGTGAGGGTCAGGAGGGTGAAATTCGTCCTCCCCAACAACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf TPR_8

Protein Analysis

588

Amino Acids

68.56

Weight (kDa)

9.81

Isoelectric Point (pI)

42.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51110
malus_domestica MD08G1044800.v1.1 MD08G1045500.v1.1
pyrus_communis pycom08g03610 pycom08g03630 pycom08g03750 pycom08g03760
rosa_samantha Rh5BG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 412, 475
AccII CGCG 5 cut(s) 267, 1467, 1647, 1712, 1719
AciI CCGC 6 cut(s) 206, 1467, 1645, 1647, 1694, 1710
AclI AACGTT 2 cut(s) 666, 1390
AclWI GGATC 4 cut(s) 179, 881, 1194, 1327
AcsI RAATTY 9 cut(s) 4, 738, 827, 941, 1065, 1157, 1352, 1564, 1739
AcuI CTGAAG 1 cut(s) 444
AfaI GTAC 2 cut(s) 131, 753
AfiI CCNNNNNNNGG 3 cut(s) 412, 475, 842
AflIII ACRYGT 3 cut(s) 157, 490, 1224
AgeI ACCGGT 1 cut(s) 555
AgsI TTSAA 6 cut(s) 92, 190, 363, 383, 731, 1575
AhdI GACNNNNNGTC 1 cut(s) 1525
AloI GAACNNNNNNTCC 2 cut(s) 1437, 1469
AluBI AGCT 5 cut(s) 591, 897, 1276, 1578, 1641
AluI AGCT 5 cut(s) 591, 897, 1276, 1578, 1641
Alw26I GTCTC 2 cut(s) 207, 1533
AlwI GGATC 4 cut(s) 179, 881, 1194, 1327
AlwNI CAGNNNCTG 1 cut(s) 1681
ApeKI GCWGC 4 cut(s) 182, 800, 894, 1641
ApoI RAATTY 9 cut(s) 4, 738, 827, 941, 1065, 1157, 1352, 1564, 1739
AseI ATTAAT 1 cut(s) 1341
AsiGI ACCGGT 1 cut(s) 555
AspLEI GCGC 4 cut(s) 225, 267, 269, 1719
AspS9I GGNCC 4 cut(s) 150, 558, 833, 1205
AsuHPI GGTGA 3 cut(s) 541, 1683, 1747
AsuII TTCGAA 1 cut(s) 1562
AvaII GGWCC 4 cut(s) 150, 558, 833, 1205
BaeGI GKGCMC 1 cut(s) 1437
BaeI ACNNNNGTAYC 2 cut(s) 1247, 1280
BbsI GAAGAC 1 cut(s) 1005
BbvI GCAGC 4 cut(s) 194, 812, 906, 1628
BccI CCATC 4 cut(s) 512, 752, 784, 925
BceAI ACGGC 1 cut(s) 994
BcoDI GTCTC 2 cut(s) 207, 1533
BfaI CTAG 3 cut(s) 95, 321, 1451
BglI GCCNNNNNGGC 1 cut(s) 37
BglII AGATCT 1 cut(s) 1105
BisI GCNGC 5 cut(s) 183, 801, 895, 1642, 1645
BlsI GCNGC 5 cut(s) 184, 802, 896, 1643, 1646
Bme18I GGWCC 4 cut(s) 150, 558, 833, 1205
BmeRI GACNNNNNGTC 1 cut(s) 1525
BmgT120I GGNCC 4 cut(s) 150, 558, 833, 1205
BmiI GGNNCC 6 cut(s) 118, 152, 560, 1082, 1206, 1207
BmsI GCATC 3 cut(s) 203, 418, 558
BpiI GAAGAC 1 cut(s) 1005
BplI GAGNNNNNCTC 2 cut(s) 1431, 1463
Bpu14I TTCGAA 1 cut(s) 1562
BpuEI CTTGAG 1 cut(s) 1070
BsaI GGTCTC 1 cut(s) 1533
BsaJI CCNNGG 2 cut(s) 836, 1645
BsaWI WCCGGW 2 cut(s) 555, 870
Bsc4I CCNNNNNNNGG 3 cut(s) 412, 475, 842
Bse118I RCCGGY 1 cut(s) 555
Bse3DI GCAATG 1 cut(s) 1322
BseDI CCNNGG 2 cut(s) 836, 1645
BseGI GGATG 2 cut(s) 523, 776
BseLI CCNNNNNNNGG 3 cut(s) 412, 475, 842
BseMI GCAATG 1 cut(s) 1322
BseMII CTCAG 3 cut(s) 240, 426, 1486
BsePI GCGCGC 1 cut(s) 265
BseRI GAGGAG 2 cut(s) 225, 606
BseSI GKGCMC 1 cut(s) 1437
BseXI GCAGC 4 cut(s) 194, 812, 906, 1628
BseYI CCCAGC 1 cut(s) 1674
BsgI GTGCAG 1 cut(s) 927
Bsh1236I CGCG 5 cut(s) 267, 1467, 1647, 1712, 1719
BshTI ACCGGT 1 cut(s) 555
BsiSI CCGG 2 cut(s) 556, 871
BslFI GGGAC 4 cut(s) 430, 544, 1191, 1532
BslI CCNNNNNNNGG 3 cut(s) 412, 475, 842
BsmAI GTCTC 2 cut(s) 207, 1533
BsmBI CGTCTC 1 cut(s) 207
BsmFI GGGAC 4 cut(s) 430, 544, 1191, 1532
BsmI GAATGC 3 cut(s) 185, 225, 258
Bso31I GGTCTC 1 cut(s) 1533
Bsp119I TTCGAA 1 cut(s) 1562
Bsp1286I GDGCHC 1 cut(s) 1437
Bsp143I GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
BspACI CCGC 6 cut(s) 206, 1467, 1645, 1647, 1694, 1710
BspCNI CTCAG 3 cut(s) 239, 427, 1485
BspFNI CGCG 5 cut(s) 267, 1467, 1647, 1712, 1719
BspHI TCATGA 2 cut(s) 337, 883
BspLI GGNNCC 6 cut(s) 118, 152, 560, 1082, 1206, 1207
BspPI GGATC 4 cut(s) 179, 881, 1194, 1327
BspT104I TTCGAA 1 cut(s) 1562
BspTNI GGTCTC 1 cut(s) 1533
BsrDI GCAATG 1 cut(s) 1322
BsrFI RCCGGY 1 cut(s) 555
BssAI RCCGGY 1 cut(s) 555
BssECI CCNNGG 2 cut(s) 836, 1645
BssHII GCGCGC 1 cut(s) 265
BssMI GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
BssT1I CCWWGG 1 cut(s) 836
Bst6I CTCTTC 1 cut(s) 1711
BstBI TTCGAA 1 cut(s) 1562
BstC8I GCNNGC 4 cut(s) 267, 910, 914, 1649
BstDEI CTNAG 4 cut(s) 142, 226, 435, 1472
BstDSI CCRYGG 1 cut(s) 1645
BstENI CCTNNNNNAGG 1 cut(s) 840
BstF5I GGATG 2 cut(s) 523, 776
BstFNI CGCG 5 cut(s) 267, 1467, 1647, 1712, 1719
BstHHI GCGC 4 cut(s) 225, 267, 269, 1719
BstKTI GATC 6 cut(s) 16, 174, 889, 1108, 1189, 1322
BstMAI GTCTC 2 cut(s) 207, 1533
BstMBI GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
BstMWI GCNNNNNNNGC 4 cut(s) 37, 64, 191, 264
BstNSI RCATGY 1 cut(s) 494
BstSLI GKGCMC 1 cut(s) 1437
BstUI CGCG 5 cut(s) 267, 1467, 1647, 1712, 1719
BstV1I GCAGC 4 cut(s) 194, 812, 906, 1628
BstV2I GAAGAC 1 cut(s) 1005
BstX2I RGATCY 1 cut(s) 1105
BstYI RGATCY 1 cut(s) 1105
BtgI CCRYGG 1 cut(s) 1645
BtsCI GGATG 2 cut(s) 523, 776
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 1 cut(s) 544
Cac8I GCNNGC 4 cut(s) 267, 910, 914, 1649
CaiI CAGNNNCTG 1 cut(s) 1681
CciI TCATGA 2 cut(s) 337, 883
CfoI GCGC 4 cut(s) 225, 267, 269, 1719
Cfr10I RCCGGY 1 cut(s) 555
Cfr13I GGNCC 4 cut(s) 150, 558, 833, 1205
Cfr42I CCGCGG 1 cut(s) 1648
Csp6I GTAC 2 cut(s) 130, 752
CspAI ACCGGT 1 cut(s) 555
CspCI CAANNNNNGTGG 2 cut(s) 1208, 1243
CviAII CATG 5 cut(s) 338, 407, 491, 578, 884
CviQI GTAC 2 cut(s) 130, 752
DdeI CTNAG 4 cut(s) 142, 226, 435, 1472
DpnI GATC 6 cut(s) 15, 173, 888, 1107, 1188, 1321
DpnII GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
DraI TTTAAA 1 cut(s) 1381
DriI GACNNNNNGTC 1 cut(s) 1525
Eam1104I CTCTTC 1 cut(s) 1711
Eam1105I GACNNNNNGTC 1 cut(s) 1525
EarI CTCTTC 1 cut(s) 1711
Eco130I CCWWGG 1 cut(s) 836
Eco31I GGTCTC 1 cut(s) 1533
Eco47I GGWCC 4 cut(s) 150, 558, 833, 1205
Eco57I CTGAAG 1 cut(s) 444
EcoNI CCTNNNNNAGG 1 cut(s) 840
EcoO109I RGGNCCY 1 cut(s) 1205
EcoRI GAATTC 1 cut(s) 1564
EcoT14I CCWWGG 1 cut(s) 836
EcoT22I ATGCAT 1 cut(s) 260
ErhI CCWWGG 1 cut(s) 836
Esp3I CGTCTC 1 cut(s) 207
FaeI CATG 5 cut(s) 341, 410, 494, 581, 887
FaqI GGGAC 4 cut(s) 430, 544, 1191, 1532
FatI CATG 5 cut(s) 337, 406, 490, 577, 883
FauI CCCGC 2 cut(s) 199, 1640
Fnu4HI GCNGC 5 cut(s) 183, 801, 895, 1642, 1645
FokI GGATG 2 cut(s) 530, 763
Fsp4HI GCNGC 5 cut(s) 183, 801, 895, 1642, 1645
FspBI CTAG 3 cut(s) 95, 321, 1451
GlaI GCGC 4 cut(s) 224, 266, 268, 1718
GluI GCNGC 5 cut(s) 183, 801, 895, 1642, 1645
GsaI CCCAGC 1 cut(s) 1678
HapII CCGG 2 cut(s) 556, 871
HhaI GCGC 4 cut(s) 225, 267, 269, 1719
Hin1II CATG 5 cut(s) 341, 410, 494, 581, 887
Hin6I GCGC 4 cut(s) 223, 265, 267, 1717
HinP1I GCGC 4 cut(s) 223, 265, 267, 1717
HincII GTYRAC 1 cut(s) 1123
HindII GTYRAC 1 cut(s) 1123
HindIII AAGCTT 1 cut(s) 1576
HinfI GANTC 5 cut(s) 138, 481, 863, 1470, 1494
HpaII CCGG 2 cut(s) 556, 871
HphI GGTGA 3 cut(s) 541, 1683, 1747
Hpy166II GTNNAC 1 cut(s) 1123
Hpy188I TCNGA 2 cut(s) 229, 760
Hpy188III TCNNGA 7 cut(s) 169, 338, 383, 681, 884, 1498, 1729
Hpy8I GTNNAC 1 cut(s) 1123
HpyAV CCTTC 6 cut(s) 267, 508, 964, 1177, 1599, 1649
HpyCH4IV ACGT 4 cut(s) 159, 666, 1224, 1390
HpyCH4V TGCA 5 cut(s) 258, 549, 581, 908, 1197
HpyF10VI GCNNNNNNNGC 4 cut(s) 37, 64, 191, 264
HpyF3I CTNAG 4 cut(s) 142, 226, 435, 1472
HpySE526I ACGT 4 cut(s) 159, 666, 1224, 1390
Hsp92II CATG 5 cut(s) 341, 410, 494, 581, 887
HspAI GCGC 4 cut(s) 223, 265, 267, 1717
KflI GGGWCCC 1 cut(s) 1205
KspI CCGCGG 1 cut(s) 1648
Kzo9I GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
LmnI GCTCC 3 cut(s) 116, 287, 1332
Lsp1109I GCAGC 4 cut(s) 194, 812, 906, 1628
LweI GCATC 3 cut(s) 203, 418, 558
MaeI CTAG 3 cut(s) 95, 321, 1451
MaeII ACGT 4 cut(s) 159, 666, 1224, 1390
MaeIII GTNAC 7 cut(s) 176, 493, 934, 1245, 1254, 1360, 1391
MalI GATC 6 cut(s) 15, 173, 888, 1107, 1188, 1321
MboI GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
MboII GAAGA 6 cut(s) 242, 428, 437, 989, 1005, 1698
MfeI CAATTG 1 cut(s) 1198
MflI RGATCY 1 cut(s) 1105
MhlI GDGCHC 1 cut(s) 1437
MlyI GAGTC 3 cut(s) 132, 872, 1464
MmeI TCCRAC 4 cut(s) 273, 891, 1160, 1262
Mph1103I ATGCAT 1 cut(s) 260
MseI TTAA 8 cut(s) 162, 441, 809, 1308, 1314, 1341, 1380, 1661
MslI CAYNNNNRTG 1 cut(s) 1096
MspA1I CMGCKG 2 cut(s) 897, 1647
MspI CCGG 2 cut(s) 556, 871
MunI CAATTG 1 cut(s) 1198
Mva1269I GAATGC 3 cut(s) 185, 225, 258
MvnI CGCG 5 cut(s) 267, 1467, 1647, 1712, 1719
MwoI GCNNNNNNNGC 4 cut(s) 37, 64, 191, 264
NdeII GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
NlaIII CATG 5 cut(s) 341, 410, 494, 581, 887
NlaIV GGNNCC 6 cut(s) 118, 152, 560, 1082, 1206, 1207
NmeAIII GCCGAG 2 cut(s) 1025, 1030
NmuCI GTSAC 2 cut(s) 1245, 1254
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 1 cut(s) 494
NspV TTCGAA 1 cut(s) 1562
PagI TCATGA 2 cut(s) 337, 883
PauI GCGCGC 1 cut(s) 265
PciI ACATGT 1 cut(s) 490
PcsI WCGNNNNNNNCGW 2 cut(s) 57, 1250
PctI GAATGC 3 cut(s) 185, 225, 258
PfeI GAWTC 2 cut(s) 481, 1494
PflMI CCANNNNNTGG 2 cut(s) 412, 475
PinAI ACCGGT 1 cut(s) 555
PkrI GCNGC 5 cut(s) 184, 802, 896, 1643, 1646
PleI GAGTC 3 cut(s) 132, 871, 1464
PpsI GAGTC 3 cut(s) 132, 871, 1464
PpuMI RGGWCCY 1 cut(s) 1205
PscI ACATGT 1 cut(s) 490
PshBI ATTAAT 1 cut(s) 1341
Psp1406I AACGTT 2 cut(s) 666, 1390
Psp5II RGGWCCY 1 cut(s) 1205
PspFI CCCAGC 1 cut(s) 1674
PspN4I GGNNCC 6 cut(s) 118, 152, 560, 1082, 1206, 1207
PspPI GGNCC 4 cut(s) 150, 558, 833, 1205
PspPPI RGGWCCY 1 cut(s) 1205
PstNI CAGNNNCTG 1 cut(s) 1681
PsuI RGATCY 1 cut(s) 1105
PteI GCGCGC 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 897
RsaI GTAC 2 cut(s) 131, 753
RsaNI GTAC 2 cut(s) 130, 752
RseI CAYNNNNRTG 1 cut(s) 1096
SacII CCGCGG 1 cut(s) 1648
SaqAI TTAA 8 cut(s) 162, 441, 809, 1308, 1314, 1341, 1380, 1661
SatI GCNGC 5 cut(s) 183, 801, 895, 1642, 1645
Sau3AI GATC 6 cut(s) 13, 171, 886, 1105, 1186, 1319
Sau96I GGNCC 4 cut(s) 150, 558, 833, 1205
SchI GAGTC 3 cut(s) 132, 872, 1464
SduI GDGCHC 1 cut(s) 1437
SfaNI GCATC 3 cut(s) 203, 418, 558
Sfr303I CCGCGG 1 cut(s) 1648
SfuI TTCGAA 1 cut(s) 1562
SgrBI CCGCGG 1 cut(s) 1648
SinI GGWCC 4 cut(s) 150, 558, 833, 1205
SmiMI CAYNNNNRTG 1 cut(s) 1096
SmlI CTYRAG 1 cut(s) 1085
SmoI CTYRAG 1 cut(s) 1085
SsiI CCGC 6 cut(s) 206, 1467, 1645, 1647, 1694, 1710
SspMI CTAG 3 cut(s) 95, 321, 1451
StyI CCWWGG 1 cut(s) 836
TaiI ACGT 4 cut(s) 162, 669, 1227, 1393
TaqI TCGA 7 cut(s) 51, 282, 507, 608, 1499, 1562, 1568
TaqII GACCGA 1 cut(s) 1515
TauI GCSGC 1 cut(s) 1647
TfiI GAWTC 2 cut(s) 481, 1494
Tru1I TTAA 8 cut(s) 162, 441, 809, 1308, 1314, 1341, 1380, 1661
Tru9I TTAA 8 cut(s) 162, 441, 809, 1308, 1314, 1341, 1380, 1661
TscAI CASTG 1 cut(s) 551
TseFI GTSAC 2 cut(s) 1245, 1254
TseI GCWGC 4 cut(s) 182, 800, 894, 1641
Tsp45I GTSAC 2 cut(s) 1245, 1254
TspGWI ACGGA 4 cut(s) 163, 231, 370, 526
TspRI CASTG 1 cut(s) 551
Van91I CCANNNNNTGG 2 cut(s) 412, 475
VpaK11BI GGWCC 4 cut(s) 150, 558, 833, 1205
VspI ATTAAT 1 cut(s) 1341
XagI CCTNNNNNAGG 1 cut(s) 840
XapI RAATTY 9 cut(s) 4, 738, 827, 941, 1065, 1157, 1352, 1564, 1739
XceI RCATGY 1 cut(s) 494
XspI CTAG 3 cut(s) 95, 321, 1451
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.