pycom08g03760

Crooked neck-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0019185
Physical Location & Seq
Forward (+)
2885080 .. 2885948
869 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 819 bp
ATGCCCGCGTCTGGATCAAGTATGCTAGGTGGGAGGAGTCCCAGAAGGAATTGGACCGTGCACGCTCTGTTTGGGAGCGGCTACTTGAGGAAGAATAAGATGATCAACCATGCGAGGAATGTGTGGAAGCGTGCTGTGCAACTCTTGCCGAGGGTGGATCAGCTTTGGTACAAATATATTCACATGGTGGAGATGATAGGGAATGTGGCTCGTGCTCGCGAAATATTTCAGAGGTGGATGGATTGGATGCCGAACCGGCAAGGCTGGATCGAATTCATCGAGTTTGAGCTTCGGTATAATGAAGTAGATCACGCGATAGCGATTTTTGAGCAGTTCGTACAATGTCATCCAAAAGCTGATGCTTGGATTCGGTATGCCAAGTTTGAGATGAAGAATGGTGACCTTGCAAGGGTAAGGGATGTGTTTCAGACGGCAGTGGATATACTAGACAATGATGAGGGGGCCGAACAGTTGTTTCTGGCTTTTGATGAATTTGAAGAAGGGTGCAAAGAAATCGAGCGTGCGAGGAGTATTTATAAGTTAGGGCGTGATCGTATACCAAAGGGAAAGGCTGATTCTTTATATAGGATGTTCGAGGAATTTGAGGAGCGGTATGAGGACAGACAAGCGATCGAGGATGCAATTTTGAACAAGGCAAGGTTCAAGTACGAGCAGGAGGTTAGGAAGAATCCTATGGATTATGATGCCTGGTTTGGTTATATAAGGCTAGAAGAAAATGCAAGGAACAAAGAGAAGATTAGACAAGTTTATGATCGAGCGATTACCAATGTTCCACCGGCTCAAGAAAAGCAGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03040 map03040
Pfam Domains
Protein Families
HAT Suf TPR_8

Protein Analysis

273

Amino Acids

32.65

Weight (kDa)

7.73

Isoelectric Point (pI)

52.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAT_Syf1_CNRKL1_N PF23233 28 - 118 2.5e-11 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
HAT_Syf1_CNRKL1_C PF23231 28 - 205 2.1e-13 Pre-mRNA-splicing factor Syf1/CRNKL1 C-terminal HAT repeat
HAT_PRP39_N PF23240 46 - 201 3.6e-13 PRP39 N-terminal HAT repeat
HAT_PRP39_C PF23241 87 - 188 6.2e-12 PRP39 C-terminal HAT repeat
HAT PF02184 101 - 129 4.1e-07 HAT (Half-A-TPR) repeat
HAT_Syf1_CNRKL1_N PF23233 221 - 265 8.5e-07 Pre-mRNA-splicing factor Syf1/CNRKL1 N-terminal HAT repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019185)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51110
malus_domestica MD08G1044800.v1.1 MD08G1045500.v1.1
pyrus_communis pycom08g03610 pycom08g03630 pycom08g03750 pycom08g03760
rosa_samantha Rh5BG087000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 537
AccBSI CCGCTC 2 cut(s) 78, 610
AccI GTMKAC 1 cut(s) 556
AccII CGCG 3 cut(s) 8, 219, 314
AciI CCGC 3 cut(s) 6, 78, 610
AclWI GGATC 3 cut(s) 22, 165, 275
AcsI RAATTY 3 cut(s) 272, 491, 599
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 3 cut(s) 170, 339, 668
AfiI CCNNNNNNNGG 2 cut(s) 11, 409
AgsI TTSAA 3 cut(s) 497, 649, 664
AjnI CCWGG 1 cut(s) 707
AluBI AGCT 3 cut(s) 163, 289, 356
AluI AGCT 3 cut(s) 163, 289, 356
Alw21I GWGCWC 2 cut(s) 63, 217
Alw44I GTGCAC 1 cut(s) 59
AlwI GGATC 3 cut(s) 22, 165, 275
AoxI GGCC 1 cut(s) 462
ApaLI GTGCAC 1 cut(s) 59
ApoI RAATTY 3 cut(s) 272, 491, 599
Asp700I GAANNNNTTC 1 cut(s) 225
AspS9I GGNCC 2 cut(s) 54, 462
AsuHPI GGTGA 1 cut(s) 410
AvaII GGWCC 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 63
BauI CACGAG 1 cut(s) 210
Bbv12I GWGCWC 2 cut(s) 63, 217
BccI CCATC 1 cut(s) 232
BceAI ACGGC 1 cut(s) 447
BcgI CGANNNNNNTGC 2 cut(s) 496, 530
BciT130I CCWGG 1 cut(s) 709
BclI TGATCA 1 cut(s) 102
BfaI CTAG 3 cut(s) 26, 446, 728
BglI GCCNNNNNGGC 1 cut(s) 256
BisI GCNGC 1 cut(s) 79
BlsI GCNGC 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 709
Bme18I GGWCC 1 cut(s) 54
BmgT120I GGNCC 2 cut(s) 54, 462
BmiI GGNNCC 1 cut(s) 463
BmrFI CCNGG 1 cut(s) 709
BmsI GCATC 4 cut(s) 237, 349, 628, 694
BpuEI CTTGAG 2 cut(s) 106, 786
BsaJI CCNNGG 1 cut(s) 149
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 409
Bse118I RCCGGY 2 cut(s) 255, 796
BseBI CCWGG 1 cut(s) 709
BseDI CCNNGG 1 cut(s) 149
BseGI GGATG 6 cut(s) 243, 252, 346, 424, 594, 643
BseLI CCNNNNNNNGG 2 cut(s) 11, 409
BseRI GAGGAG 3 cut(s) 49, 541, 620
BseSI GKGCMC 1 cut(s) 63
Bsh1236I CGCG 3 cut(s) 8, 219, 314
Bsh1285I CGRYCG 1 cut(s) 633
BshFI GGCC 1 cut(s) 464
BsiEI CGRYCG 1 cut(s) 633
BsiHKAI GWGCWC 2 cut(s) 63, 217
BsiSI CCGG 2 cut(s) 256, 797
BslFI GGGAC 1 cut(s) 24
BslI CCNNNNNNNGG 2 cut(s) 11, 409
BsmFI GGGAC 1 cut(s) 24
BsnI GGCC 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 63, 217
Bsp143I GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
Bsp68I TCGCGA 1 cut(s) 219
BspACI CCGC 3 cut(s) 6, 78, 610
BspANI GGCC 1 cut(s) 464
BspFNI CGCG 3 cut(s) 8, 219, 314
BspLI GGNNCC 1 cut(s) 463
BspPI GGATC 3 cut(s) 22, 165, 275
BsrBI CCGCTC 2 cut(s) 78, 610
BsrFI RCCGGY 2 cut(s) 255, 796
BssAI RCCGGY 2 cut(s) 255, 796
BssECI CCNNGG 1 cut(s) 149
BssMI GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
BssNAI GTATAC 1 cut(s) 557
BssSI CACGAG 1 cut(s) 210
Bst1107I GTATAC 1 cut(s) 557
Bst2BI CACGAG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 709
Bst4CI ACNGT 2 cut(s) 58, 471
BstAPI GCANNNNNTGC 1 cut(s) 145
BstC8I GCNNGC 5 cut(s) 6, 63, 132, 217, 522
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 6 cut(s) 243, 252, 346, 424, 594, 643
BstFNI CGCG 3 cut(s) 8, 219, 314
BstKTI GATC 8 cut(s) 17, 105, 160, 270, 310, 553, 633, 775
BstMBI GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
BstMCI CGRYCG 1 cut(s) 633
BstMWI GCNNNNNNNGC 3 cut(s) 136, 145, 256
BstNI CCWGG 1 cut(s) 709
BstPI GGTNACC 1 cut(s) 398
BstSCI CCNGG 1 cut(s) 707
BstSLI GKGCMC 1 cut(s) 63
BstUI CGCG 3 cut(s) 8, 219, 314
BstZ17I GTATAC 1 cut(s) 557
BsuRI GGCC 1 cut(s) 464
BtsCI GGATG 6 cut(s) 243, 252, 346, 424, 594, 643
BtsI GCAGTG 1 cut(s) 441
BtsIMutI CAGTG 1 cut(s) 441
BtuMI TCGCGA 1 cut(s) 219
Cac8I GCNNGC 5 cut(s) 6, 63, 132, 217, 522
Cfr10I RCCGGY 2 cut(s) 255, 796
Cfr13I GGNCC 2 cut(s) 54, 462
Csp6I GTAC 3 cut(s) 169, 338, 667
CviAII CATG 2 cut(s) 110, 184
CviQI GTAC 3 cut(s) 169, 338, 667
DpnI GATC 8 cut(s) 16, 104, 159, 269, 309, 552, 632, 774
DpnII GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
DraIII CACNNNGTG 1 cut(s) 187
Eco47I GGWCC 1 cut(s) 54
Eco91I GGTNACC 1 cut(s) 398
EcoO65I GGTNACC 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 272
EcoRII CCWGG 1 cut(s) 707
FaeI CATG 2 cut(s) 113, 187
FaqI GGGAC 1 cut(s) 24
FatI CATG 2 cut(s) 109, 183
FauI CCCGC 1 cut(s) 13
FbaI TGATCA 1 cut(s) 102
FblI GTMKAC 1 cut(s) 556
Fnu4HI GCNGC 1 cut(s) 79
FokI GGATG 6 cut(s) 250, 259, 333, 431, 601, 650
Fsp4HI GCNGC 1 cut(s) 79
FspBI CTAG 3 cut(s) 26, 446, 728
GluI GCNGC 1 cut(s) 79
HaeIII GGCC 1 cut(s) 464
HapII CCGG 2 cut(s) 256, 797
Hin1II CATG 2 cut(s) 113, 187
HinfI GANTC 4 cut(s) 37, 367, 575, 688
HpaII CCGG 2 cut(s) 256, 797
HphI GGTGA 1 cut(s) 410
Hpy166II GTNNAC 2 cut(s) 61, 557
Hpy188I TCNGA 2 cut(s) 231, 429
Hpy188III TCNNGA 3 cut(s) 12, 218, 803
Hpy8I GTNNAC 2 cut(s) 61, 557
HpyAV CCTTC 2 cut(s) 39, 494
HpyCH4III ACNGT 2 cut(s) 58, 471
HpyCH4V TGCA 6 cut(s) 61, 139, 407, 507, 641, 740
HpyF10VI GCNNNNNNNGC 3 cut(s) 136, 145, 256
Hsp92II CATG 2 cut(s) 113, 187
Ksp22I TGATCA 1 cut(s) 102
Kzo9I GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
LmnI GCTCC 2 cut(s) 75, 607
LpnPI CCDG 8 cut(s) 55, 250, 269, 464, 659, 694, 721, 810
LweI GCATC 4 cut(s) 237, 349, 628, 694
MaeI CTAG 3 cut(s) 26, 446, 728
MaeIII GTNAC 1 cut(s) 398
MalI GATC 8 cut(s) 16, 104, 159, 269, 309, 552, 632, 774
MbiI CCGCTC 2 cut(s) 78, 610
MboI GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
MboII GAAGA 6 cut(s) 103, 403, 509, 697, 743, 766
MhlI GDGCHC 2 cut(s) 63, 217
MluCI AATT 5 cut(s) 49, 272, 491, 599, 642
MlyI GAGTC 1 cut(s) 46
MroXI GAANNNNTTC 1 cut(s) 225
MspI CCGG 2 cut(s) 256, 797
MspR9I CCNGG 1 cut(s) 709
MvaI CCWGG 1 cut(s) 709
MvnI CGCG 3 cut(s) 8, 219, 314
MwoI GCNNNNNNNGC 3 cut(s) 136, 145, 256
NdeII GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
NlaIII CATG 2 cut(s) 113, 187
NlaIV GGNNCC 1 cut(s) 463
NmeAIII GCCGAG 1 cut(s) 174
NmuCI GTSAC 1 cut(s) 398
NruI TCGCGA 1 cut(s) 219
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 3 cut(s) 367, 575, 688
PkrI GCNGC 1 cut(s) 80
Ple19I CGATCG 1 cut(s) 633
PleI GAGTC 1 cut(s) 45
PpsI GAGTC 1 cut(s) 45
PsiI TTATAA 1 cut(s) 537
Psp6I CCWGG 1 cut(s) 707
PspEI GGTNACC 1 cut(s) 398
PspGI CCWGG 1 cut(s) 707
PspN4I GGNNCC 1 cut(s) 463
PspPI GGNCC 2 cut(s) 54, 462
PvuI CGATCG 1 cut(s) 633
RruI TCGCGA 1 cut(s) 219
RsaI GTAC 3 cut(s) 170, 339, 668
RsaNI GTAC 3 cut(s) 169, 338, 667
SatI GCNGC 1 cut(s) 79
Sau3AI GATC 8 cut(s) 14, 102, 157, 267, 307, 550, 630, 772
Sau96I GGNCC 2 cut(s) 54, 462
SchI GAGTC 1 cut(s) 46
ScrFI CCNGG 1 cut(s) 709
SduI GDGCHC 2 cut(s) 63, 217
SetI ASST 8 cut(s) 31, 165, 236, 291, 358, 405, 662, 681
SfaNI GCATC 4 cut(s) 237, 349, 628, 694
SinI GGWCC 1 cut(s) 54
SmlI CTYRAG 2 cut(s) 85, 801
SmoI CTYRAG 2 cut(s) 85, 801
Sse9I AATT 5 cut(s) 49, 272, 491, 599, 642
SsiI CCGC 3 cut(s) 6, 78, 610
SspI AATATT 1 cut(s) 225
SspMI CTAG 3 cut(s) 26, 446, 728
StyD4I CCNGG 1 cut(s) 707
TaaI ACNGT 2 cut(s) 58, 471
TaqI TCGA 6 cut(s) 270, 279, 516, 594, 633, 775
TasI AATT 5 cut(s) 49, 272, 491, 599, 642
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 3 cut(s) 367, 575, 688
TscAI CASTG 1 cut(s) 441
TseFI GTSAC 1 cut(s) 398
Tsp45I GTSAC 1 cut(s) 398
TspDTI ATGAA 4 cut(s) 265, 315, 404, 504
TspRI CASTG 1 cut(s) 441
VneI GTGCAC 1 cut(s) 59
VpaK11BI GGWCC 1 cut(s) 54
XapI RAATTY 3 cut(s) 272, 491, 599
XmiI GTMKAC 1 cut(s) 556
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 3 cut(s) 26, 446, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.