pycom08g06720

Galactose oxidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0022343
Physical Location & Seq
Forward (+)
5257297 .. 5257971
675 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 675 bp
ATGCCTCAAGCTAGAGTCATGAGTGACATGTTATTGCTTCCAGATGGCACCGTTTTGATCATCAACGGTGCATCTTTTGGGACTGCGGGTTGGGAACTCGGACAGAATCCTGTGCTCGAACCACTTGTGTACCGACCAGATAACGCTGTTGGGTCACGGTTTGAAAAACAAAACCCTGCCCCAATACCGCGAATGTACCATTCCACCGCGGTATTACTACGTGATGGTAGGGTACTCGTTGGAGGTAGTAACCCTCATGATAAATACGAGTTAGCCGATGAGTTATTTCCAACAGAATTGAGATTAGAGGCATTTAGTCCTGATTATTTGGATGCCAAGTACTCGAAGTTGCGTCCGAGAATCGTTGATCCCAAATCACAAGCTAAAATCAGTTACGGAAAAAAATTATTCATTCGGTTTTCGTTGAAGGGAAGCATAGCAACGAATTTGGTGATGGTGACGATAGTGGCGCCGTCATTTAATACACATTCCTTCTCAATGAATCAGAGGCTGCTGGTTCTTGCGGCGGAGACCGTCAAAAAGGTAGGGACAATGACGTACCAAGTTCAGGTGACGACGCCGGGTTCCGGTAATCTTGCGCCTTCCGGATATTATCTTCTCTACGTGGTGCATCAGCAAATTCCAAGTGAAGGCATCTGGGTTCAAATTCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1929 Glyoxal_oxid_N

Protein Analysis

225

Amino Acids

24.79

Weight (kDa)

9.22

Isoelectric Point (pI)

23.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyoxal_oxid_N PF07250 1 - 109 5.3e-50 Glyoxal oxidase N-terminus
GO-like_E_set PF09118 118 - 223 5.7e-28 Galactose oxidase-like, Early set domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022343)

Species Orthologous Gene IDs
malus_domestica MD15G1068800.v1.1
pyrus_communis pycom08g06720
rosa_multiflora Rmu_co7995866.1_g000001
rosa_roxburghii Rroxscaffold_7G00165910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 47, 469
AccII CGCG 2 cut(s) 190, 209
AccIII TCCGGA 1 cut(s) 605
AciI CCGC 6 cut(s) 86, 188, 207, 209, 524, 527
AclWI GGATC 1 cut(s) 362
AcsI RAATTY 3 cut(s) 445, 639, 666
AcyI GRCGYC 2 cut(s) 470, 578
AfaI GTAC 5 cut(s) 131, 197, 234, 341, 560
AfiI CCNNNNNNNGG 3 cut(s) 568, 587, 650
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 3 cut(s) 164, 427, 665
AluBI AGCT 2 cut(s) 11, 383
AluI AGCT 2 cut(s) 11, 383
Alw21I GWGCWC 1 cut(s) 117
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 1 cut(s) 362
AlwNI CAGNNNCTG 1 cut(s) 511
Aor13HI TCCGGA 1 cut(s) 605
ApeKI GCWGC 1 cut(s) 511
ApoI RAATTY 3 cut(s) 445, 639, 666
AspLEI GCGC 2 cut(s) 472, 601
AsuC2I CCSGG 1 cut(s) 582
AsuHPI GGTGA 3 cut(s) 463, 469, 583
BaeI ACNNNNGTAYC 2 cut(s) 113, 146
BanI GGYRCC 2 cut(s) 47, 469
Bbv12I GWGCWC 1 cut(s) 117
BbvI GCAGC 1 cut(s) 498
BccI CCATC 3 cut(s) 38, 218, 448
BceAI ACGGC 1 cut(s) 457
BclI TGATCA 1 cut(s) 57
BcnI CCSGG 1 cut(s) 582
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 473
BisI GCNGC 2 cut(s) 512, 525
BlsI GCNGC 2 cut(s) 513, 526
BmcAI AGTACT 1 cut(s) 341
Bme1390I CCNGG 1 cut(s) 582
BmiI GGNNCC 3 cut(s) 49, 471, 586
BmrFI CCNGG 1 cut(s) 582
BmsI GCATC 4 cut(s) 80, 322, 640, 663
BpuMI CCSGG 1 cut(s) 582
BsaAI YACGTR 2 cut(s) 221, 625
BsaHI GRCGYC 2 cut(s) 470, 578
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 1 cut(s) 207
BsaWI WCCGGW 2 cut(s) 587, 605
Bsc4I CCNNNNNNNGG 3 cut(s) 568, 587, 650
BseAI TCCGGA 1 cut(s) 605
BseDI CCNNGG 1 cut(s) 207
BseGI GGATG 1 cut(s) 337
BseLI CCNNNNNNNGG 3 cut(s) 568, 587, 650
BseXI GCAGC 1 cut(s) 498
Bsh1236I CGCG 2 cut(s) 190, 209
BshNI GGYRCC 2 cut(s) 47, 469
BsiHKAI GWGCWC 1 cut(s) 117
BsiSI CCGG 3 cut(s) 581, 588, 606
BslFI GGGAC 2 cut(s) 94, 562
BslI CCNNNNNNNGG 3 cut(s) 568, 587, 650
BsmAI GTCTC 1 cut(s) 524
BsmFI GGGAC 2 cut(s) 94, 562
Bso31I GGTCTC 1 cut(s) 524
Bsp1286I GDGCHC 1 cut(s) 117
Bsp13I TCCGGA 1 cut(s) 605
Bsp143I GATC 2 cut(s) 57, 367
BspACI CCGC 6 cut(s) 86, 188, 207, 209, 524, 527
BspEI TCCGGA 1 cut(s) 605
BspFNI CGCG 2 cut(s) 190, 209
BspHI TCATGA 2 cut(s) 18, 256
BspLI GGNNCC 3 cut(s) 49, 471, 586
BspPI GGATC 1 cut(s) 362
BspT107I GGYRCC 2 cut(s) 47, 469
BspTNI GGTCTC 1 cut(s) 524
BssECI CCNNGG 1 cut(s) 207
BssMI GATC 2 cut(s) 57, 367
BssNI GRCGYC 2 cut(s) 470, 578
Bst4CI ACNGT 4 cut(s) 52, 68, 159, 535
BstACI GRCGYC 2 cut(s) 470, 578
BstBAI YACGTR 2 cut(s) 221, 625
BstDSI CCRYGG 1 cut(s) 207
BstF5I GGATG 1 cut(s) 337
BstFNI CGCG 2 cut(s) 190, 209
BstH2I RGCGCY 1 cut(s) 473
BstHHI GCGC 2 cut(s) 472, 601
BstKTI GATC 2 cut(s) 60, 370
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 2 cut(s) 57, 367
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 1 cut(s) 580
BstUI CGCG 2 cut(s) 190, 209
BstV1I GCAGC 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 207
BtsCI GGATG 1 cut(s) 337
CaiI CAGNNNCTG 1 cut(s) 511
CciI TCATGA 2 cut(s) 18, 256
CfoI GCGC 2 cut(s) 472, 601
Cfr42I CCGCGG 1 cut(s) 210
CseI GACGC 2 cut(s) 341, 586
Csp6I GTAC 5 cut(s) 130, 196, 233, 340, 559
CviAII CATG 3 cut(s) 19, 28, 257
CviJI RGCY 4 cut(s) 11, 275, 383, 511
CviKI_1 RGCY 4 cut(s) 11, 275, 383, 511
CviQI GTAC 5 cut(s) 130, 196, 233, 340, 559
DinI GGCGCC 1 cut(s) 471
DpnI GATC 2 cut(s) 59, 369
DpnII GATC 2 cut(s) 57, 367
EciI GGCGGA 1 cut(s) 542
Eco31I GGTCTC 1 cut(s) 524
EgeI GGCGCC 1 cut(s) 471
EheI GGCGCC 1 cut(s) 471
FaeI CATG 3 cut(s) 22, 31, 260
FaiI YATR 4 cut(s) 20, 29, 258, 437
FaqI GGGAC 2 cut(s) 94, 562
FatI CATG 3 cut(s) 18, 27, 256
FauI CCCGC 1 cut(s) 79
FbaI TGATCA 1 cut(s) 57
Fnu4HI GCNGC 2 cut(s) 512, 525
FokI GGATG 1 cut(s) 344
Fsp4HI GCNGC 2 cut(s) 512, 525
FspBI CTAG 1 cut(s) 12
GlaI GCGC 2 cut(s) 471, 600
GluI GCNGC 2 cut(s) 512, 525
HaeII RGCGCY 1 cut(s) 473
HapII CCGG 3 cut(s) 581, 588, 606
HgaI GACGC 2 cut(s) 341, 586
HhaI GCGC 2 cut(s) 472, 601
Hin1I GRCGYC 2 cut(s) 470, 578
Hin1II CATG 3 cut(s) 22, 31, 260
Hin6I GCGC 2 cut(s) 470, 599
HinP1I GCGC 2 cut(s) 470, 599
HinfI GANTC 4 cut(s) 15, 106, 360, 502
HpaII CCGG 3 cut(s) 581, 588, 606
HphI GGTGA 3 cut(s) 463, 469, 583
Hpy166II GTNNAC 1 cut(s) 130
Hpy188I TCNGA 3 cut(s) 101, 357, 507
Hpy188III TCNNGA 5 cut(s) 19, 41, 257, 320, 606
Hpy8I GTNNAC 1 cut(s) 130
Hpy99I CGWCG 1 cut(s) 580
HpyAV CCTTC 4 cut(s) 421, 502, 612, 644
HpyCH4III ACNGT 4 cut(s) 52, 68, 159, 535
HpyCH4IV ACGT 3 cut(s) 220, 557, 624
HpyCH4V TGCA 2 cut(s) 71, 631
HpySE526I ACGT 3 cut(s) 220, 557, 624
Hsp92I GRCGYC 2 cut(s) 470, 578
Hsp92II CATG 3 cut(s) 22, 31, 260
HspAI GCGC 2 cut(s) 470, 599
KasI GGCGCC 1 cut(s) 469
Kpn2I TCCGGA 1 cut(s) 605
Ksp22I TGATCA 1 cut(s) 57
KspI CCGCGG 1 cut(s) 210
Kzo9I GATC 2 cut(s) 57, 367
Lsp1109I GCAGC 1 cut(s) 498
LweI GCATC 4 cut(s) 80, 322, 640, 663
MaeI CTAG 1 cut(s) 12
MaeII ACGT 3 cut(s) 220, 557, 624
MaeIII GTNAC 6 cut(s) 23, 153, 248, 392, 457, 571
MalI GATC 2 cut(s) 59, 369
MboI GATC 2 cut(s) 57, 367
MboII GAAGA 1 cut(s) 608
MhlI GDGCHC 1 cut(s) 117
MluCI AATT 5 cut(s) 296, 404, 445, 639, 666
Mly113I GGCGCC 1 cut(s) 470
MlyI GAGTC 1 cut(s) 24
MmeI TCCRAC 2 cut(s) 220, 314
MnlI CCTC 5 cut(s) 15, 236, 264, 301, 501
MroI TCCGGA 1 cut(s) 605
MseI TTAA 1 cut(s) 480
MspA1I CMGCKG 1 cut(s) 209
MspI CCGG 3 cut(s) 581, 588, 606
MspR9I CCNGG 1 cut(s) 582
MvnI CGCG 2 cut(s) 190, 209
NarI GGCGCC 1 cut(s) 470
NciI CCSGG 1 cut(s) 582
NdeII GATC 2 cut(s) 57, 367
NlaIII CATG 3 cut(s) 22, 31, 260
NlaIV GGNNCC 3 cut(s) 49, 471, 586
NmuCI GTSAC 4 cut(s) 23, 153, 457, 571
NspI RCATGY 1 cut(s) 31
PagI TCATGA 2 cut(s) 18, 256
PciI ACATGT 1 cut(s) 27
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PfeI GAWTC 3 cut(s) 106, 360, 502
PkrI GCNGC 2 cut(s) 513, 526
PleI GAGTC 1 cut(s) 23
PluTI GGCGCC 1 cut(s) 473
PpsI GAGTC 1 cut(s) 23
Ppu21I YACGTR 2 cut(s) 221, 625
PscI ACATGT 1 cut(s) 27
PspN4I GGNNCC 3 cut(s) 49, 471, 586
PstNI CAGNNNCTG 1 cut(s) 511
RsaI GTAC 5 cut(s) 131, 197, 234, 341, 560
RsaNI GTAC 5 cut(s) 130, 196, 233, 340, 559
SacII CCGCGG 1 cut(s) 210
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 2 cut(s) 512, 525
Sau3AI GATC 2 cut(s) 57, 367
ScaI AGTACT 1 cut(s) 341
SchI GAGTC 1 cut(s) 24
ScrFI CCNGG 1 cut(s) 582
SduI GDGCHC 1 cut(s) 117
SetI ASST 8 cut(s) 13, 223, 247, 385, 546, 560, 573, 627
SfaNI GCATC 4 cut(s) 80, 322, 640, 663
SfoI GGCGCC 1 cut(s) 471
Sfr303I CCGCGG 1 cut(s) 210
SgrBI CCGCGG 1 cut(s) 210
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
Sse9I AATT 5 cut(s) 296, 404, 445, 639, 666
SsiI CCGC 6 cut(s) 86, 188, 207, 209, 524, 527
SspDI GGCGCC 1 cut(s) 469
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 580
TaaI ACNGT 4 cut(s) 52, 68, 159, 535
TaiI ACGT 3 cut(s) 223, 560, 627
TaqI TCGA 2 cut(s) 117, 344
TasI AATT 5 cut(s) 296, 404, 445, 639, 666
TatI WGTACW 1 cut(s) 339
TauI GCSGC 1 cut(s) 527
TfiI GAWTC 3 cut(s) 106, 360, 502
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TseFI GTSAC 4 cut(s) 23, 153, 457, 571
TseI GCWGC 1 cut(s) 511
Tsp45I GTSAC 4 cut(s) 23, 153, 457, 571
TspDTI ATGAA 2 cut(s) 400, 515
TspGWI ACGGA 1 cut(s) 411
XapI RAATTY 3 cut(s) 445, 639, 666
XceI RCATGY 1 cut(s) 31
XspI CTAG 1 cut(s) 12
ZrmI AGTACT 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.