pycom08g08740

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0020856
Physical Location & Seq
Forward (+)
7053639 .. 7053935
297 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 297 bp
ATGGCCAATGGCAAGCTCATCGTTCTTCTTCTTTTTCTAATTTTCTCTTATGGGATCATTTCAACTGAAGAAAGGTCGCTAAAAACAGATCATACTAAAGGGGGATCGACATCAATCAGCAGCAACAATTATCTCAACTCGCGCCGAAACATGTTGGAAAGTGAATTATCAGACTCTGTGACACCGGTTCTTGGCTATGAGAGTGCTCCTACTGACAATTTCCGGCCCACGACGCCAGGGTACAGTCCCGGTGCTGGTCATTCTACTGGACCTCAAGTAGCAGAGCCAAACCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

10.53

Weight (kDa)

5.53

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020856)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47485
prunus_persica Prupe.1G440600_v2.0.a1
pyrus_communis pycom08g08740
rosa_laevigata RLG00000010730
rosa_roxburghii Rroxscaffold_7G00161110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 142
AclWI GGATC 2 cut(s) 62, 112
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 87
AcyI GRCGYC 1 cut(s) 233
AfaI GTAC 1 cut(s) 242
AfiI CCNNNNNNNGG 2 cut(s) 191, 254
AflIII ACRYGT 1 cut(s) 150
AgeI ACCGGT 1 cut(s) 184
AgsI TTSAA 1 cut(s) 63
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
Alw21I GWGCWC 1 cut(s) 208
AlwI GGATC 2 cut(s) 62, 112
AlwNI CAGNNNCTG 1 cut(s) 176
AoxI GGCC 2 cut(s) 3, 224
ApeKI GCWGC 1 cut(s) 120
AsiGI ACCGGT 1 cut(s) 184
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 2 cut(s) 225, 269
AsuC2I CCSGG 1 cut(s) 249
AvaII GGWCC 1 cut(s) 269
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 208
BbvI GCAGC 1 cut(s) 132
BcgI CGANNNNNNTGC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 237
BcnI CCSGG 1 cut(s) 249
BisI GCNGC 1 cut(s) 121
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 2 cut(s) 237, 249
Bme18I GGWCC 1 cut(s) 269
BmgT120I GGNCC 2 cut(s) 225, 269
BmrFI CCNGG 2 cut(s) 237, 249
BpuEI CTTGAG 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 249
BsaBI GATNNNNATC 1 cut(s) 109
BsaHI GRCGYC 1 cut(s) 233
BsaJI CCNNGG 1 cut(s) 236
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 254
Bse118I RCCGGY 1 cut(s) 184
Bse1I ACTGG 2 cut(s) 271, 292
Bse8I GATNNNNATC 1 cut(s) 109
BseBI CCWGG 1 cut(s) 237
BseDI CCNNGG 1 cut(s) 236
BseJI GATNNNNATC 1 cut(s) 109
BseLI CCNNNNNNNGG 2 cut(s) 191, 254
BseNI ACTGG 2 cut(s) 271, 292
BseXI GCAGC 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 142
BshFI GGCC 2 cut(s) 5, 226
BshTI ACCGGT 1 cut(s) 184
BsiHKAI GWGCWC 1 cut(s) 208
BsiSI CCGG 3 cut(s) 185, 223, 249
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 2 cut(s) 191, 254
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 2 cut(s) 5, 226
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 3 cut(s) 54, 88, 104
BspANI GGCC 2 cut(s) 5, 226
BspFNI CGCG 1 cut(s) 142
BspPI GGATC 2 cut(s) 62, 112
BsrFI RCCGGY 1 cut(s) 184
BsrI ACTGG 2 cut(s) 271, 292
BssAI RCCGGY 1 cut(s) 184
BssECI CCNNGG 1 cut(s) 236
BssMI GATC 3 cut(s) 54, 88, 104
BssNI GRCGYC 1 cut(s) 233
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 1 cut(s) 245
BstACI GRCGYC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 14
BstFNI CGCG 1 cut(s) 142
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 3 cut(s) 57, 91, 107
BstMBI GATC 3 cut(s) 54, 88, 104
BstMWI GCNNNNNNNGC 1 cut(s) 232
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 2 cut(s) 235, 247
BstUI CGCG 1 cut(s) 142
BstV1I GCAGC 1 cut(s) 132
BsuRI GGCC 2 cut(s) 5, 226
Cac8I GCNNGC 1 cut(s) 14
CaiI CAGNNNCTG 1 cut(s) 176
CfoI GCGC 1 cut(s) 144
Cfr10I RCCGGY 1 cut(s) 184
Cfr13I GGNCC 2 cut(s) 225, 269
CseI GACGC 1 cut(s) 241
Csp6I GTAC 1 cut(s) 241
CspAI ACCGGT 1 cut(s) 184
CviAII CATG 1 cut(s) 151
CviJI RGCY 5 cut(s) 5, 16, 195, 226, 286
CviKI_1 RGCY 5 cut(s) 5, 16, 195, 226, 286
CviQI GTAC 1 cut(s) 241
DpnI GATC 3 cut(s) 56, 90, 106
DpnII GATC 3 cut(s) 54, 88, 104
EaeI YGGCCR 1 cut(s) 3
Eco47I GGWCC 1 cut(s) 269
Eco57I CTGAAG 1 cut(s) 87
EcoRII CCWGG 1 cut(s) 235
FaeI CATG 1 cut(s) 154
FaiI YATR 4 cut(s) 51, 93, 152, 198
FaqI GGGAC 1 cut(s) 231
FatI CATG 1 cut(s) 150
Fnu4HI GCNGC 1 cut(s) 121
Fsp4HI GCNGC 1 cut(s) 121
GlaI GCGC 1 cut(s) 143
GluI GCNGC 1 cut(s) 121
HaeIII GGCC 2 cut(s) 5, 226
HapII CCGG 3 cut(s) 185, 223, 249
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 144
Hin1I GRCGYC 1 cut(s) 233
Hin1II CATG 1 cut(s) 154
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HinfI GANTC 1 cut(s) 173
HpaII CCGG 3 cut(s) 185, 223, 249
Hpy188I TCNGA 1 cut(s) 172
Hpy99I CGWCG 1 cut(s) 235
HpyCH4III ACNGT 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 1 cut(s) 232
Hsp92I GRCGYC 1 cut(s) 233
Hsp92II CATG 1 cut(s) 154
HspAI GCGC 1 cut(s) 142
Kzo9I GATC 3 cut(s) 54, 88, 104
LmnI GCTCC 1 cut(s) 211
LpnPI CCDG 7 cut(s) 198, 222, 236, 240, 249, 252, 262
Lsp1109I GCAGC 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 178
MalI GATC 3 cut(s) 56, 90, 106
MboI GATC 3 cut(s) 54, 88, 104
MboII GAAGA 3 cut(s) 17, 20, 80
MhlI GDGCHC 1 cut(s) 208
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 39, 127, 164, 217
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 167
MmeI TCCRAC 1 cut(s) 135
MnlI CCTC 1 cut(s) 282
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 185, 223, 249
MspR9I CCNGG 2 cut(s) 237, 249
MvaI CCWGG 1 cut(s) 237
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 1 cut(s) 232
NciI CCSGG 1 cut(s) 249
NdeII GATC 3 cut(s) 54, 88, 104
NlaIII CATG 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 178
NspI RCATGY 1 cut(s) 154
PciI ACATGT 1 cut(s) 150
PinAI ACCGGT 1 cut(s) 184
PkrI GCNGC 1 cut(s) 122
PleI GAGTC 1 cut(s) 167
PpsI GAGTC 1 cut(s) 167
PscI ACATGT 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 235
PspGI CCWGG 1 cut(s) 235
PspPI GGNCC 2 cut(s) 225, 269
PstNI CAGNNNCTG 1 cut(s) 176
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 3 cut(s) 54, 88, 104
Sau96I GGNCC 2 cut(s) 225, 269
SchI GAGTC 1 cut(s) 167
ScrFI CCNGG 2 cut(s) 237, 249
SduI GDGCHC 1 cut(s) 208
SetI ASST 3 cut(s) 18, 77, 274
SinI GGWCC 1 cut(s) 269
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 4 cut(s) 39, 127, 164, 217
StyD4I CCNGG 2 cut(s) 235, 247
TaaI ACNGT 1 cut(s) 245
TaqI TCGA 1 cut(s) 107
TasI AATT 4 cut(s) 39, 127, 164, 217
TseFI GTSAC 1 cut(s) 178
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 178
VpaK11BI GGWCC 1 cut(s) 269
XceI RCATGY 1 cut(s) 154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.