Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr8

Orthogroup ID

OG0014569

Physical Location & Seq

Genomic Coordinates Forward (+)

8036306 .. 8041133

Gene Structure 4828 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom08g09690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGCACAGCTTTTGGTGGCTCCTTGGAGTTCTTCACCATGTCATTTGGGTTGCAACCATGCTCATTACCAGCCAAGCCATGCCGCCACCGCCCCCGATTCAATGCGACGCATCAACCGGCTGCAACCTCTCCAACTCCTACGGGGTATGGGGTGACAGAACAGATTGCCATGTTCCATCCGCCGCACACCCACAAACGGAGGAAGACCTCCGCCTCGCTGTGGCTTTTGCGAGCAAAAACAAGATCAAGATCAAAGTGGTGAGCAAATTTTCACATACCATACCAAAGTTGGCATGTCCTGACATGAGTTTTGGAGACGCAATGCTAATAAGCACGGCTAATGTTAATTCCACAATCGAAATCGACGCAGCGAATTTAGCTGTCACGGTGGATGCTGGCGTAGGGCTACGTCAGTTGATCGACAAAGTGGAGGAAGCTGGGCTGAGCTTGGTGGCTGCGCCGTATTGGGAGGGTGTGAGCGTAGGAGGGCTTATAAGCACGGGGGCGCATGGAAGCTCGTGGCGGGGAAGAGGAGGAGCTGTGCATGATCATGTTATTGGTCTCAATCTTGTTGTTCCAGCAAATCAATCGGAAGGGTATGCCAAAGTTCTCAGGTTAGATTCTAAGGATGAAATCTTCAAAGCTGCCAAAGTTTCATTGGGGTTGCTGGGTATCATCTCCAAGGTGAAATTGAGATTGGAGCCAGCATTCAAAAGAAGCATAGCCTATAACTTTACAGACGATGCTCAAATTGAGGACATATACATGGACCATGCACAAAAATATGAGTTTGGAGACATAACATGGTATCCATCGAAGCATACGGCTGCATACAGATATGATAACAGACTTCCTTTAACCGCATCAGGCGATGGAACATACGATTTTTTAGGCTTCCAGTCCAATTCTGTTGTGGTCTCCAAGACTATAAGACTAACAGAGAAAACAATCGACAGCGGACGAAGCTCGATTGGGAAATGCACACTAGCATCTACAGTTTTGGAGGCCAAGAAATTAGTGGCCAATGGTTTGAAAAATAACCTAATCTTCACTGGGTACCCAGTTGTAGGTCATCAGGGTAAAATGCAAACCTCAGGTTCATGTCTGTACTCGGCAGGCACAGACAAATCCTGTGCGTGGGACCCAAGAATCAACGGCCTCTTCTTCTACGAGACAACGGCAATATTTCCAGCATCAAAATTTGCAGACTTCATCCGAGACGTCAAAAAACTACGAGACCTTAAACCAGAAAACTTCTGTGGGGTGGATATTTACAATGGTTTTCTAATCAGATTCATTAGGGTTTCCGATGCGTATCTTGGTCAACAAGAAAACTCCGTGGTCGTTGACTTTAATTACTACAGAGCCGATGATGCTTCGACTCCAAGGTTCAATCAAGACGTGTGGGAAGAGGTGGAGCAACTGGCGTTTTTCAAGCATGGGGCAAAGCCACACTGGGCTAAAAACAGAAACGCAGCGTTTTTGGACGTGCAGAAAAAGTACCCCAATTTTAACAAGTTTTTGGGTGTGAAAAGACGGTTGGATCCTCAAAATATATTGTCGAGCGAATGGTCGGATGAGATCTTATCTGGGAAAGAAGGTGCAAAGCGTGACGGGTGTGCTTTGGAGGGTCTTTGTGTATGTTCTGAAGATCGGCATTGCAGCCCGGCGAAAGGGTATTTCTGTAAACCTGGACTTGTTTATAAGGAGGCTAGAGTTTGTAGGTACTCATCATCACCATCCTCTGTGGGATCAGAGAGTTACAAAACTGATGCATTATTTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005975 GO:0005996 GO:0006082 GO:0006732 GO:0006766 GO:0006767 GO:0008150 GO:0008152 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016051 GO:0016053 GO:0016491 GO:0016614 GO:0016899 GO:0019752 GO:0019852 GO:0019853 GO:0036094 GO:0042364 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044281 GO:0044283 GO:0046364 GO:0046394 GO:0048037 GO:0050105 GO:0050660 GO:0050662 GO:0051186 GO:0051188 GO:0055114 GO:0071704 GO:0097159 GO:1901265 GO:1901363 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALO FAD_binding_4

Protein Analysis

596

Amino Acids

65.62

Weight (kDa)

7.84

Isoelectric Point (pI)

36.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	62 - 193	1.1e-17	FAD binding domain
ALO	PF04030	397 - 527	3.9e-14	D-arabinono-1,4-lactone oxidase
GULLO2-like_3rd	PF22906	537 - 576	2.2e-23	L-gulonolactone oxidase 2-like, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014569)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G11540
fragaria_vesca	FvH4_2g35870
malus_domestica	MD08G1116100.v1.1 MD15G1095800.v1.1
prunus_persica	Prupe.1G450800_v2.0.a1
pyrus_communis	pycom08g09690 pycom15g08810
rosa_chinensis	RchiOBHm_Chr6g0305951
rosa_laevigata	RLG00000010843
rosa_multiflora	Rmu_sc0001809.1_g000053
rosa_roxburghii	Rroxscaffold_7G00162190
rosa_rugosa	Rorug06G0345600
rosa_samantha	Rh6AG457300 Rh6BG443800 Rh6CG471300 Rh6DG457800
rosa_wichuraiana	Rw6G039900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	494, 1704
AatII	GACGTC	1 cut(s)	1224
Acc65I	GGTACC	1 cut(s)	1056
AccB1I	GGYRCC	1 cut(s)	1056
AciI	CCGC	8 cut(s)	83, 89, 180, 183, 211, 523, 861, 957
AclWI	GGATC	3 cut(s)	1538, 1551, 1759
AcoI	YGGCCR	1 cut(s)	1020
AcsI	RAATTY	3 cut(s)	266, 373, 1199
AcuI	CTGAAG	1 cut(s)	1668
AcyI	GRCGYC	1 cut(s)	1221
AfaI	GTAC	4 cut(s)	1058, 1109, 1502, 1727
AfiI	CCNNNNNNNGG	5 cut(s)	196, 220, 1067, 1137, 1673
AflIII	ACRYGT	1 cut(s)	1401
AgsI	TTSAA	6 cut(s)	101, 640, 712, 1033, 1393, 1435
AjiI	CACGTC	2 cut(s)	1402, 1489
AjnI	CCWGG	1 cut(s)	1690
AjuI	GAANNNNNNNTTGG	4 cut(s)	680, 712, 1523, 1555
AluBI	AGCT	8 cut(s)	9, 380, 437, 447, 516, 539, 644, 966
AluI	AGCT	8 cut(s)	9, 380, 437, 447, 516, 539, 644, 966
Alw26I	GTCTC	7 cut(s)	309, 566, 789, 922, 1166, 1212, 1230
AlwI	GGATC	3 cut(s)	1538, 1551, 1759
AoxI	GGCC	3 cut(s)	1005, 1020, 1156
ApeKI	GCWGC	7 cut(s)	120, 368, 455, 644, 827, 1475, 1662
ApoI	RAATTY	3 cut(s)	266, 373, 1199
ArsI	GACNNNNNNTTYG	2 cut(s)	293, 325
Asp718I	GGTACC	1 cut(s)	1056
AspLEI	GCGC	2 cut(s)	460, 508
AspS9I	GGNCC	2 cut(s)	769, 1141
AsuC2I	CCSGG	1 cut(s)	1667
AsuHPI	GGTGA	5 cut(s)	26, 164, 271, 697, 1728
AvaII	GGWCC	2 cut(s)	769, 1141
AxyI	CCTNAGG	1 cut(s)	1093
BalI	TGGCCA	1 cut(s)	1022
BamHI	GGATCC	1 cut(s)	1543
BanI	GGYRCC	1 cut(s)	1056
BauI	CACGAG	1 cut(s)	517
BbsI	GAAGAC	1 cut(s)	210
BbvI	GCAGC	7 cut(s)	107, 380, 442, 631, 814, 1487, 1674
BccI	CCATC	4 cut(s)	184, 820, 866, 1747
BceAI	ACGGC	5 cut(s)	351, 445, 840, 1171, 1194
BcgI	CGANNNNNNTGC	2 cut(s)	570, 604
BciT130I	CCWGG	1 cut(s)	1692
BciVI	GTATCC	1 cut(s)	819
BclI	TGATCA	1 cut(s)	547
BcnI	CCSGG	1 cut(s)	1667
BcoDI	GTCTC	7 cut(s)	309, 566, 789, 922, 1166, 1212, 1230
BfaI	CTAG	2 cut(s)	986, 1713
BfmI	CTRYAG	2 cut(s)	993, 1360
BfuI	GTATCC	1 cut(s)	819
BglII	AGATCT	1 cut(s)	1581
BisI	GCNGC	9 cut(s)	83, 121, 183, 369, 456, 645, 828, 1476, 1663
BlpI	GCTNAGC	1 cut(s)	443
BlsI	GCNGC	9 cut(s)	84, 122, 184, 370, 457, 646, 829, 1477, 1664
Bme1390I	CCNGG	2 cut(s)	1667, 1692
Bme18I	GGWCC	2 cut(s)	769, 1141
BmgBI	CACGTC	2 cut(s)	1402, 1489
BmgT120I	GGNCC	2 cut(s)	769, 1141
BmiI	GGNNCC	6 cut(s)	20, 702, 1058, 1142, 1143, 1545
BmrFI	CCNGG	2 cut(s)	1667, 1692
BmrI	ACTGGG	3 cut(s)	1055, 1062, 1465
BmsI	GCATC	9 cut(s)	119, 382, 733, 872, 998, 1202, 1300, 1363, 1762
BmuI	ACTGGG	3 cut(s)	1055, 1062, 1465
BpiI	GAAGAC	1 cut(s)	210
Bpu1102I	GCTNAGC	1 cut(s)	443
BpuMI	CCSGG	1 cut(s)	1667
BsaBI	GATNNNNATC	2 cut(s)	248, 1314
BsaHI	GRCGYC	1 cut(s)	1221
BsaI	GGTCTC	3 cut(s)	566, 922, 1230
BsaJI	CCNNGG	4 cut(s)	22, 681, 1338, 1385
BsaXI	ACNNNNNCTCC	2 cut(s)	525, 555
Bsc4I	CCNNNNNNNGG	5 cut(s)	196, 220, 1067, 1137, 1673
Bse118I	RCCGGY	1 cut(s)	116
Bse1I	ACTGG	5 cut(s)	898, 1057, 1061, 1428, 1460
Bse21I	CCTNAGG	1 cut(s)	1093
Bse3DI	GCAATG	2 cut(s)	327, 1657
Bse8I	GATNNNNATC	2 cut(s)	248, 1314
BseBI	CCWGG	1 cut(s)	1692
BseDI	CCNNGG	4 cut(s)	22, 681, 1338, 1385
BseGI	GGATG	6 cut(s)	176, 397, 634, 1212, 1582, 1739
BseJI	GATNNNNATC	2 cut(s)	248, 1314
BseLI	CCNNNNNNNGG	5 cut(s)	196, 220, 1067, 1137, 1673
BseMI	GCAATG	2 cut(s)	327, 1657
BseMII	CTCAG	3 cut(s)	434, 625, 1107
BseNI	ACTGG	5 cut(s)	898, 1057, 1061, 1428, 1460
BseRI	GAGGAG	2 cut(s)	546, 549
BseXI	GCAGC	7 cut(s)	107, 380, 442, 631, 814, 1487, 1674
BseYI	CCCAGC	2 cut(s)	437, 667
BsgI	GTGCAG	1 cut(s)	1511
BshFI	GGCC	3 cut(s)	1007, 1022, 1158
BshNI	GGYRCC	1 cut(s)	1056
BsiSI	CCGG	2 cut(s)	117, 1667
BslFI	GGGAC	1 cut(s)	1154
BslI	CCNNNNNNNGG	5 cut(s)	196, 220, 1067, 1137, 1673
BsmAI	GTCTC	7 cut(s)	309, 566, 789, 922, 1166, 1212, 1230
BsmBI	CGTCTC	2 cut(s)	309, 1212
BsmFI	GGGAC	1 cut(s)	1154
BsmI	GAATGC	1 cut(s)	707
BsnI	GGCC	3 cut(s)	1007, 1022, 1158
Bso31I	GGTCTC	3 cut(s)	566, 922, 1230
Bsp143I	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
Bsp1720I	GCTNAGC	1 cut(s)	443
BspACI	CCGC	8 cut(s)	83, 89, 180, 183, 211, 523, 861, 957
BspANI	GGCC	3 cut(s)	1007, 1022, 1158
BspCNI	CTCAG	3 cut(s)	435, 624, 1106
BspLI	GGNNCC	6 cut(s)	20, 702, 1058, 1142, 1143, 1545
BspPI	GGATC	3 cut(s)	1538, 1551, 1759
BspT107I	GGYRCC	1 cut(s)	1056
BspTNI	GGTCTC	3 cut(s)	566, 922, 1230
BsrDI	GCAATG	2 cut(s)	327, 1657
BsrFI	RCCGGY	1 cut(s)	116
BsrI	ACTGG	5 cut(s)	898, 1057, 1061, 1428, 1460
BssAI	RCCGGY	1 cut(s)	116
BssECI	CCNNGG	4 cut(s)	22, 681, 1338, 1385
BssMI	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
BssNI	GRCGYC	1 cut(s)	1221
BssSI	CACGAG	1 cut(s)	517
BssT1I	CCWWGG	3 cut(s)	22, 681, 1385
Bst2BI	CACGAG	1 cut(s)	517
Bst2UI	CCWGG	1 cut(s)	1692
Bst4CI	ACNGT	3 cut(s)	388, 997, 1539
Bst6I	CTCTTC	3 cut(s)	523, 1166, 1404
BstACI	GRCGYC	1 cut(s)	1221
BstC8I	GCNNGC	4 cut(s)	232, 397, 705, 1117
BstDEI	CTNAG	4 cut(s)	443, 611, 624, 1093
BstDSI	CCRYGG	1 cut(s)	1338
BstF5I	GGATG	6 cut(s)	176, 397, 634, 1212, 1582, 1739
BstHHI	GCGC	2 cut(s)	460, 508
BstKTI	GATC	8 cut(s)	246, 252, 420, 550, 1546, 1584, 1654, 1754
BstMAI	GTCTC	7 cut(s)	309, 566, 789, 922, 1166, 1212, 1230
BstMBI	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
BstMWI	GCNNNNNNNGC	4 cut(s)	88, 377, 963, 1373
BstNI	CCWGG	1 cut(s)	1692
BstNSI	RCATGY	1 cut(s)	297
BstSCI	CCNGG	2 cut(s)	1665, 1690
BstSFI	CTRYAG	2 cut(s)	993, 1360
BstV1I	GCAGC	7 cut(s)	107, 380, 442, 631, 814, 1487, 1674
BstV2I	GAAGAC	1 cut(s)	210
BstX2I	RGATCY	2 cut(s)	1543, 1581
BstYI	RGATCY	2 cut(s)	1543, 1581
Bsu36I	CCTNAGG	1 cut(s)	1093
BsuI	GTATCC	1 cut(s)	819
BsuRI	GGCC	3 cut(s)	1007, 1022, 1158
BtgI	CCRYGG	1 cut(s)	1338
BtgZI	GCGATG	1 cut(s)	885
BtrI	CACGTC	2 cut(s)	1402, 1489
BtsCI	GGATG	6 cut(s)	176, 397, 634, 1212, 1582, 1739
BtsIMutI	CAGTG	2 cut(s)	1050, 1453
Cac8I	GCNNGC	4 cut(s)	232, 397, 705, 1117
CfoI	GCGC	2 cut(s)	460, 508
Cfr10I	RCCGGY	1 cut(s)	116
Cfr13I	GGNCC	2 cut(s)	769, 1141
CseI	GACGC	3 cut(s)	116, 326, 374
Csp6I	GTAC	4 cut(s)	1057, 1108, 1501, 1726
CspCI	CAANNNNNGTGG	2 cut(s)	1385, 1420
CviQI	GTAC	4 cut(s)	1057, 1108, 1501, 1726
DdeI	CTNAG	4 cut(s)	443, 611, 624, 1093
DpnI	GATC	8 cut(s)	245, 251, 419, 549, 1545, 1583, 1653, 1753
DpnII	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
EaeI	YGGCCR	1 cut(s)	1020
Eam1104I	CTCTTC	3 cut(s)	523, 1166, 1404
EarI	CTCTTC	3 cut(s)	523, 1166, 1404
EciI	GGCGGA	2 cut(s)	169, 200
Eco130I	CCWWGG	3 cut(s)	22, 681, 1385
Eco31I	GGTCTC	3 cut(s)	566, 922, 1230
Eco47I	GGWCC	2 cut(s)	769, 1141
Eco57I	CTGAAG	1 cut(s)	1668
Eco81I	CCTNAGG	1 cut(s)	1093
EcoO109I	RGGNCCY	1 cut(s)	1141
EcoRII	CCWGG	1 cut(s)	1690
EcoT14I	CCWWGG	3 cut(s)	22, 681, 1385
EcoT22I	ATGCAT	1 cut(s)	1777
ErhI	CCWWGG	3 cut(s)	22, 681, 1385
Esp3I	CGTCTC	2 cut(s)	309, 1212
FaqI	GGGAC	1 cut(s)	1154
FauI	CCCGC	1 cut(s)	516
FbaI	TGATCA	1 cut(s)	547
Fnu4HI	GCNGC	9 cut(s)	83, 121, 183, 369, 456, 645, 828, 1476, 1663
FokI	GGATG	6 cut(s)	163, 404, 641, 1199, 1589, 1726
Fsp4HI	GCNGC	9 cut(s)	83, 121, 183, 369, 456, 645, 828, 1476, 1663
FspBI	CTAG	2 cut(s)	986, 1713
GlaI	GCGC	2 cut(s)	459, 507
GluI	GCNGC	9 cut(s)	83, 121, 183, 369, 456, 645, 828, 1476, 1663
GsaI	CCCAGC	2 cut(s)	441, 671
HaeIII	GGCC	3 cut(s)	1007, 1022, 1158
HapII	CCGG	2 cut(s)	117, 1667
HgaI	GACGC	3 cut(s)	116, 326, 374
HhaI	GCGC	2 cut(s)	460, 508
Hin1I	GRCGYC	1 cut(s)	1221
Hin6I	GCGC	2 cut(s)	458, 506
HinP1I	GCGC	2 cut(s)	458, 506
HincII	GTYRAC	2 cut(s)	1325, 1348
HindII	GTYRAC	2 cut(s)	1325, 1348
HinfI	GANTC	5 cut(s)	97, 620, 1149, 1293, 1381
HpaII	CCGG	2 cut(s)	117, 1667
HphI	GGTGA	5 cut(s)	26, 164, 271, 697, 1728
Hpy166II	GTNNAC	3 cut(s)	1325, 1348, 1688
Hpy188I	TCNGA	7 cut(s)	592, 1217, 1292, 1309, 1576, 1648, 1756
Hpy188III	TCNNGA	3 cut(s)	247, 299, 1397
Hpy8I	GTNNAC	3 cut(s)	1325, 1348, 1688
Hpy99I	CGWCG	2 cut(s)	110, 368
HpyAV	CCTTC	2 cut(s)	587, 1592
HpyCH4III	ACNGT	3 cut(s)	388, 997, 1539
HpyCH4IV	ACGT	4 cut(s)	409, 1221, 1401, 1488
HpyF10VI	GCNNNNNNNGC	4 cut(s)	88, 377, 963, 1373
HpyF3I	CTNAG	4 cut(s)	443, 611, 624, 1093
HpySE526I	ACGT	4 cut(s)	409, 1221, 1401, 1488
Hsp92I	GRCGYC	1 cut(s)	1221
HspAI	GCGC	2 cut(s)	458, 506
KflI	GGGWCCC	1 cut(s)	1141
KpnI	GGTACC	1 cut(s)	1060
Ksp22I	TGATCA	1 cut(s)	547
Kzo9I	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
LmnI	GCTCC	4 cut(s)	24, 536, 700, 1417
Lsp1109I	GCAGC	7 cut(s)	107, 380, 442, 631, 814, 1487, 1674
LweI	GCATC	9 cut(s)	119, 382, 733, 872, 998, 1202, 1300, 1363, 1762
MaeI	CTAG	2 cut(s)	986, 1713
MaeII	ACGT	4 cut(s)	409, 1221, 1401, 1488
MaeIII	GTNAC	4 cut(s)	152, 382, 1610, 1760
MalI	GATC	8 cut(s)	245, 251, 419, 549, 1545, 1583, 1653, 1753
MboI	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
MboII	GAAGA	9 cut(s)	23, 215, 540, 628, 1039, 1153, 1156, 1421, 1661
MflI	RGATCY	2 cut(s)	1543, 1581
MlsI	TGGCCA	1 cut(s)	1022
MluNI	TGGCCA	1 cut(s)	1022
MlyI	GAGTC	1 cut(s)	1375
MmeI	TCCRAC	3 cut(s)	156, 1521, 1554
Mox20I	TGGCCA	1 cut(s)	1022
Mph1103I	ATGCAT	1 cut(s)	1777
MscI	TGGCCA	1 cut(s)	1022
MseI	TTAA	6 cut(s)	345, 857, 1242, 1353, 1512, 1786
MslI	CAYNNNNRTG	2 cut(s)	549, 764
Msp20I	TGGCCA	1 cut(s)	1022
MspA1I	CMGCKG	1 cut(s)	957
MspI	CCGG	2 cut(s)	117, 1667
MspR9I	CCNGG	2 cut(s)	1667, 1692
Mva1269I	GAATGC	1 cut(s)	707
MvaI	CCWGG	1 cut(s)	1692
MwoI	GCNNNNNNNGC	4 cut(s)	88, 377, 963, 1373
NciI	CCSGG	1 cut(s)	1667
NdeII	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
NlaIV	GGNNCC	6 cut(s)	20, 702, 1058, 1142, 1143, 1545
NmeAIII	GCCGAG	1 cut(s)	1091
NmuCI	GTSAC	3 cut(s)	152, 382, 1610
NsiI	ATGCAT	1 cut(s)	1777
NspI	RCATGY	1 cut(s)	297
PctI	GAATGC	1 cut(s)	707
PfeI	GAWTC	4 cut(s)	97, 620, 1149, 1293
PkrI	GCNGC	9 cut(s)	84, 122, 184, 370, 457, 646, 829, 1477, 1664
PleI	GAGTC	1 cut(s)	1375
PpsI	GAGTC	1 cut(s)	1375
PpuMI	RGGWCCY	1 cut(s)	1141
PsiI	TTATAA	2 cut(s)	494, 1704
Psp5II	RGGWCCY	1 cut(s)	1141
Psp6I	CCWGG	1 cut(s)	1690
PspFI	CCCAGC	2 cut(s)	437, 667
PspGI	CCWGG	1 cut(s)	1690
PspN4I	GGNNCC	6 cut(s)	20, 702, 1058, 1142, 1143, 1545
PspPI	GGNCC	2 cut(s)	769, 1141
PspPPI	RGGWCCY	1 cut(s)	1141
PsuI	RGATCY	2 cut(s)	1543, 1581
RsaI	GTAC	4 cut(s)	1058, 1109, 1502, 1727
RsaNI	GTAC	4 cut(s)	1057, 1108, 1501, 1726
RseI	CAYNNNNRTG	2 cut(s)	549, 764
SaqAI	TTAA	6 cut(s)	345, 857, 1242, 1353, 1512, 1786
SatI	GCNGC	9 cut(s)	83, 121, 183, 369, 456, 645, 828, 1476, 1663
Sau3AI	GATC	8 cut(s)	243, 249, 417, 547, 1543, 1581, 1651, 1751
Sau96I	GGNCC	2 cut(s)	769, 1141
SchI	GAGTC	1 cut(s)	1375
ScrFI	CCNGG	2 cut(s)	1667, 1692
SfaNI	GCATC	9 cut(s)	119, 382, 733, 872, 998, 1202, 1300, 1363, 1762
SfcI	CTRYAG	2 cut(s)	993, 1360
SinI	GGWCC	2 cut(s)	769, 1141
SmiMI	CAYNNNNRTG	2 cut(s)	549, 764
SsiI	CCGC	8 cut(s)	83, 89, 180, 183, 211, 523, 861, 957
SspI	AATATT	1 cut(s)	1185
SspMI	CTAG	2 cut(s)	986, 1713
StyD4I	CCNGG	2 cut(s)	1665, 1690
StyI	CCWWGG	3 cut(s)	22, 681, 1385
TaaI	ACNGT	3 cut(s)	388, 997, 1539
TaiI	ACGT	4 cut(s)	412, 1224, 1404, 1491
TaqI	TCGA	8 cut(s)	357, 363, 420, 815, 951, 968, 1379, 1562
TatI	WGTACW	1 cut(s)	1107
TauI	GCSGC	2 cut(s)	85, 185
TfiI	GAWTC	4 cut(s)	97, 620, 1149, 1293
Tru1I	TTAA	6 cut(s)	345, 857, 1242, 1353, 1512, 1786
Tru9I	TTAA	6 cut(s)	345, 857, 1242, 1353, 1512, 1786
TscAI	CASTG	2 cut(s)	1057, 1460
TseFI	GTSAC	3 cut(s)	152, 382, 1610
TseI	GCWGC	7 cut(s)	120, 368, 455, 644, 827, 1475, 1662
Tsp45I	GTSAC	3 cut(s)	152, 382, 1610
TspDTI	ATGAA	5 cut(s)	645, 645, 1089, 1201, 1285
TspGWI	ACGGA	2 cut(s)	212, 1327
TspRI	CASTG	2 cut(s)	1057, 1460
VpaK11BI	GGWCC	2 cut(s)	769, 1141
XapI	RAATTY	3 cut(s)	266, 373, 1199
XceI	RCATGY	1 cut(s)	297
XcmI	CCANNNNNNNNNTGG	4 cut(s)	286, 655, 910, 1015
XspI	CTAG	2 cut(s)	986, 1713
ZraI	GACGTC	1 cut(s)	1222
Zsp2I	ATGCAT	1 cut(s)	1777

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.