pycom09g10270

Carbon-nitrogen hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0013731
Physical Location & Seq
Reverse (-)
8395513 .. 8397669
2157 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10270.1

Sequence Viewer

Length: 921 bp
ATGGCATCTCTCTGGAACACCAGTAATCCCGCCATGGCTGATGCCTCAGATCCATTTCAACTCCCCAATATTCCAAAGTTTAAGGTTGCTCTATGTCAGTTGTCGGTTACTTCTGACAAGAATCAGAATCTTGATCGTGCCGGCATTTCGATAAAAACTGCCGCCGAGCAAGGCGCAAAGCTACTTGTTTTACCAGAAATGTGGAACTGCTCTTATTCAAGTGAGTACTTCGCAAAATTTGCTGAGGATTTTACGAATGAGGATGCCTCGCCGACGTTGTCCATGCTATCGGAGGCTGCTTATTGCCATGGGATCACAATCGTAGGAGGGTCGGTGCCTGAACGGGATCATGGCCGGTTGTATAATACTTGCTGCATATTTGGACCAGATGGAAAGCTCAAGGCTAAGCATAGGAAAATACATTTATTCGACATTGACGTTCCAGGAGAGATATCATTTAAGGAATCTGACTTCTTTTCGGCAGGAGATCAAACTACCATAGTGGACACAGAAGTTGGTCGTATCGGAGTGGGAATCTGTCATGACTTACGGTTTCCTGAACTTGCTGCATTGTACAGGGAAAAAGGCGTTCATATCATATGCTATCCCGGGGCATTCAACATGAGCACTGGTGAATTGTTTTGGGAGCTCGTACAGAGAGCAAGGGCGATTGATAACCAGTTATTCGTAGCTACTTGTTCACCTTCTCGAGACTCCACTGGTTCTTACACAATATGGGGACACTCCATGCTAGTTGAACCGTCGGGCGAGATAATTGCAACCGCAGGGCATGAGGAAACTATTGTAATCGCTGAGATCGATTACTCTAAAATTCAGCTCCAAAGAAAGAGCCTCCAACTTGATGAACAAAAGTGGGAAGACATCTACAAGTGGATCGATGCGCATGAACCAAGTCCTTGA

Protein Analysis

307

Amino Acids

34.01

Weight (kDa)

5.0

Isoelectric Point (pI)

32.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CN_hydrolase PF00795 28 - 282 1e-66 Carbon-nitrogen hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 903
AccB1I GGYRCC 1 cut(s) 334
AciI CCGC 3 cut(s) 30, 162, 783
AclWI GGATC 4 cut(s) 44, 320, 354, 902
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 2 cut(s) 236, 831
AfaI GTAC 3 cut(s) 227, 575, 654
AgsI TTSAA 4 cut(s) 59, 219, 619, 758
AjnI CCWGG 1 cut(s) 442
AluBI AGCT 5 cut(s) 181, 397, 649, 692, 838
AluI AGCT 5 cut(s) 181, 397, 649, 692, 838
Alw21I GWGCWC 2 cut(s) 629, 651
Alw26I GTCTC 1 cut(s) 705
AlwI GGATC 4 cut(s) 44, 320, 354, 902
Ama87I CYCGRG 2 cut(s) 608, 708
AoxI GGCC 1 cut(s) 352
ApeKI GCWGC 3 cut(s) 296, 372, 566
ApoI RAATTY 2 cut(s) 236, 831
AspLEI GCGC 2 cut(s) 176, 904
AspS9I GGNCC 1 cut(s) 383
AsuC2I CCSGG 2 cut(s) 609, 610
AsuHPI GGTGA 2 cut(s) 644, 693
AvaI CYCGRG 2 cut(s) 608, 708
AvaII GGWCC 1 cut(s) 383
BanI GGYRCC 1 cut(s) 334
BanII GRGCYC 1 cut(s) 651
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 2 cut(s) 629, 651
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 283, 359, 553
BccI CCATC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 758, 792
BciT130I CCWGG 1 cut(s) 444
BcnI CCSGG 2 cut(s) 609, 610
BcoDI GTCTC 1 cut(s) 705
BfaI CTAG 1 cut(s) 752
BisI GCNGC 4 cut(s) 162, 297, 373, 567
BlpI GCTNAGC 1 cut(s) 405
BlsI GCNGC 4 cut(s) 163, 298, 374, 568
BmcAI AGTACT 1 cut(s) 227
Bme1390I CCNGG 3 cut(s) 444, 609, 610
Bme18I GGWCC 1 cut(s) 383
BmeT110I CYCGRG 2 cut(s) 608, 708
BmgT120I GGNCC 1 cut(s) 383
BmiI GGNNCC 1 cut(s) 336
BmrFI CCNGG 3 cut(s) 444, 609, 610
BmsI GCATC 4 cut(s) 14, 31, 253, 889
BpiI GAAGAC 1 cut(s) 885
BplI GAGNNNNNCTC 2 cut(s) 251, 283
Bpu10I CCTNAGC 1 cut(s) 243
Bpu1102I GCTNAGC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 2 cut(s) 609, 610
Bsa29I ATCGAT 2 cut(s) 819, 897
BsaBI GATNNNNATC 1 cut(s) 317
BsaJI CCNNGG 4 cut(s) 33, 307, 608, 609
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bse118I RCCGGY 2 cut(s) 140, 354
Bse1I ACTGG 4 cut(s) 21, 634, 679, 724
Bse8I GATNNNNATC 1 cut(s) 317
BseBI CCWGG 1 cut(s) 444
BseCI ATCGAT 2 cut(s) 819, 897
BseDI CCNNGG 4 cut(s) 33, 307, 608, 609
BseGI GGATG 1 cut(s) 268
BseJI GATNNNNATC 1 cut(s) 317
BseMII CTCAG 3 cut(s) 60, 234, 804
BseNI ACTGG 4 cut(s) 21, 634, 679, 724
BseXI GCAGC 3 cut(s) 283, 359, 553
BshFI GGCC 1 cut(s) 354
BshNI GGYRCC 1 cut(s) 334
BshVI ATCGAT 2 cut(s) 819, 897
BsiHKAI GWGCWC 2 cut(s) 629, 651
BsiHKCI CYCGRG 2 cut(s) 608, 708
BsiSI CCGG 3 cut(s) 141, 355, 609
BslFI GGGAC 1 cut(s) 753
BsmAI GTCTC 1 cut(s) 705
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 1 cut(s) 614
BsnI GGCC 1 cut(s) 354
BsoBI CYCGRG 2 cut(s) 608, 708
Bsp1286I GDGCHC 2 cut(s) 629, 651
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
Bsp1720I GCTNAGC 1 cut(s) 405
Bsp19I CCATGG 2 cut(s) 33, 307
BspACI CCGC 3 cut(s) 30, 162, 783
BspANI GGCC 1 cut(s) 354
BspCNI CTCAG 3 cut(s) 59, 235, 805
BspDI ATCGAT 2 cut(s) 819, 897
BspHI TCATGA 1 cut(s) 541
BspLI GGNNCC 1 cut(s) 336
BspPI GGATC 4 cut(s) 44, 320, 354, 902
BspT107I GGYRCC 1 cut(s) 334
BsrFI RCCGGY 2 cut(s) 140, 354
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 4 cut(s) 21, 634, 679, 724
BssAI RCCGGY 2 cut(s) 140, 354
BssECI CCNNGG 4 cut(s) 33, 307, 608, 609
BssMI GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
BssT1I CCWWGG 2 cut(s) 33, 307
Bst2UI CCWGG 1 cut(s) 444
Bst4CI ACNGT 2 cut(s) 552, 762
BstAPI GCANNNNNTGC 1 cut(s) 239
BstAUI TGTACA 1 cut(s) 573
BstC8I GCNNGC 1 cut(s) 142
BstDEI CTNAG 4 cut(s) 46, 243, 405, 813
BstDSI CCRYGG 2 cut(s) 33, 307
BstF5I GGATG 1 cut(s) 268
BstHHI GCGC 2 cut(s) 176, 904
BstKTI GATC 7 cut(s) 52, 136, 315, 349, 490, 819, 897
BstMAI GTCTC 1 cut(s) 705
BstMBI GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
BstMWI GCNNNNNNNGC 1 cut(s) 239
BstNI CCWGG 1 cut(s) 444
BstSCI CCNGG 3 cut(s) 442, 607, 608
BstV1I GCAGC 3 cut(s) 283, 359, 553
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 1 cut(s) 49
BstXI CCANNNNNNTGG 1 cut(s) 201
BstYI RGATCY 1 cut(s) 49
Bsu15I ATCGAT 2 cut(s) 819, 897
BsuRI GGCC 1 cut(s) 354
BsuTUI ATCGAT 2 cut(s) 819, 897
BtgI CCRYGG 2 cut(s) 33, 307
BtsCI GGATG 1 cut(s) 268
BtsIMutI CAGTG 2 cut(s) 627, 717
Cac8I GCNNGC 1 cut(s) 142
CciI TCATGA 1 cut(s) 541
CfoI GCGC 2 cut(s) 176, 904
Cfr10I RCCGGY 2 cut(s) 140, 354
Cfr13I GGNCC 1 cut(s) 383
Cfr9I CCCGGG 1 cut(s) 608
ClaI ATCGAT 2 cut(s) 819, 897
Csp6I GTAC 3 cut(s) 226, 574, 653
CviAII CATG 9 cut(s) 34, 283, 308, 350, 542, 622, 748, 791, 905
CviQI GTAC 3 cut(s) 226, 574, 653
DdeI CTNAG 4 cut(s) 46, 243, 405, 813
DpnI GATC 7 cut(s) 51, 135, 314, 348, 489, 818, 896
DpnII GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
EaeI YGGCCR 1 cut(s) 352
Ecl136II GAGCTC 1 cut(s) 649
Eco130I CCWWGG 2 cut(s) 33, 307
Eco24I GRGCYC 1 cut(s) 651
Eco32I GATATC 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 383
Eco53kI GAGCTC 1 cut(s) 649
Eco88I CYCGRG 2 cut(s) 608, 708
EcoICRI GAGCTC 1 cut(s) 649
EcoRII CCWGG 1 cut(s) 442
EcoRV GATATC 1 cut(s) 453
EcoT14I CCWWGG 2 cut(s) 33, 307
EcoT38I GRGCYC 1 cut(s) 651
ErhI CCWWGG 2 cut(s) 33, 307
FaeI CATG 9 cut(s) 37, 286, 311, 353, 545, 625, 751, 794, 908
FaqI GGGAC 1 cut(s) 753
FatI CATG 9 cut(s) 33, 282, 307, 349, 541, 621, 747, 790, 904
FauI CCCGC 1 cut(s) 37
FauNDI CATATG 1 cut(s) 599
Fnu4HI GCNGC 4 cut(s) 162, 297, 373, 567
FokI GGATG 1 cut(s) 275
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 4 cut(s) 162, 297, 373, 567
FspAI RTGCGCAY 1 cut(s) 903
FspBI CTAG 1 cut(s) 752
FspI TGCGCA 1 cut(s) 903
GlaI GCGC 2 cut(s) 175, 903
GluI GCNGC 4 cut(s) 162, 297, 373, 567
HaeIII GGCC 1 cut(s) 354
HapII CCGG 3 cut(s) 141, 355, 609
HhaI GCGC 2 cut(s) 176, 904
Hin1II CATG 9 cut(s) 37, 286, 311, 353, 545, 625, 751, 794, 908
Hin6I GCGC 2 cut(s) 174, 902
HinP1I GCGC 2 cut(s) 174, 902
HinfI GANTC 5 cut(s) 121, 127, 464, 534, 713
HpaII CCGG 3 cut(s) 141, 355, 609
HphI GGTGA 2 cut(s) 644, 693
Hpy166II GTNNAC 2 cut(s) 505, 701
Hpy188I TCNGA 6 cut(s) 49, 115, 126, 292, 469, 527
Hpy188III TCNNGA 6 cut(s) 13, 131, 542, 557, 708, 710
Hpy8I GTNNAC 2 cut(s) 505, 701
Hpy99I CGWCG 2 cut(s) 277, 766
HpyAV CCTTC 1 cut(s) 714
HpyCH4III ACNGT 2 cut(s) 552, 762
HpyCH4IV ACGT 2 cut(s) 275, 438
HpyCH4V TGCA 3 cut(s) 375, 569, 779
HpyF10VI GCNNNNNNNGC 1 cut(s) 239
HpyF3I CTNAG 4 cut(s) 46, 243, 405, 813
HpySE526I ACGT 2 cut(s) 275, 438
Hsp92II CATG 9 cut(s) 37, 286, 311, 353, 545, 625, 751, 794, 908
HspAI GCGC 2 cut(s) 174, 902
KroI GCCGGC 1 cut(s) 140
KroNI GCCGGC 1 cut(s) 142
Kzo9I GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
LmnI GCTCC 2 cut(s) 646, 843
Lsp1109I GCAGC 3 cut(s) 283, 359, 553
LweI GCATC 4 cut(s) 14, 31, 253, 889
MaeI CTAG 1 cut(s) 752
MaeII ACGT 2 cut(s) 275, 438
MaeIII GTNAC 1 cut(s) 106
MalI GATC 7 cut(s) 51, 135, 314, 348, 489, 818, 896
MboI GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
MboII GAAGA 1 cut(s) 890
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 2 cut(s) 629, 651
MluCI AATT 4 cut(s) 236, 635, 774, 831
MlyI GAGTC 1 cut(s) 707
MmeI TCCRAC 1 cut(s) 880
MnlI CCTC 8 cut(s) 55, 238, 253, 277, 286, 320, 787, 863
MroNI GCCGGC 1 cut(s) 140
MseI TTAA 2 cut(s) 81, 459
MspI CCGG 3 cut(s) 141, 355, 609
MspR9I CCNGG 3 cut(s) 444, 609, 610
Mva1269I GAATGC 1 cut(s) 614
MvaI CCWGG 1 cut(s) 444
MwoI GCNNNNNNNGC 1 cut(s) 239
NaeI GCCGGC 1 cut(s) 142
NciI CCSGG 2 cut(s) 609, 610
NcoI CCATGG 2 cut(s) 33, 307
NdeI CATATG 1 cut(s) 599
NdeII GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
NgoMIV GCCGGC 1 cut(s) 140
NlaIII CATG 9 cut(s) 37, 286, 311, 353, 545, 625, 751, 794, 908
NlaIV GGNNCC 1 cut(s) 336
NmeAIII GCCGAG 1 cut(s) 190
NsbI TGCGCA 1 cut(s) 903
PaeR7I CTCGAG 1 cut(s) 708
PagI TCATGA 1 cut(s) 541
PcsI WCGNNNNNNNCGW 2 cut(s) 435, 816
PctI GAATGC 1 cut(s) 614
PdiI GCCGGC 1 cut(s) 142
PfeI GAWTC 4 cut(s) 121, 127, 464, 534
PflFI GACNNNGTC 1 cut(s) 277
PfoI TCCNGGA 1 cut(s) 442
PkrI GCNGC 4 cut(s) 163, 298, 374, 568
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
Psp124BI GAGCTC 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 442
PspGI CCWGG 1 cut(s) 442
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 1 cut(s) 383
PsuI RGATCY 1 cut(s) 49
PsyI GACNNNGTC 1 cut(s) 277
RsaI GTAC 3 cut(s) 227, 575, 654
RsaNI GTAC 3 cut(s) 226, 574, 653
SacI GAGCTC 1 cut(s) 651
SaqAI TTAA 2 cut(s) 81, 459
SatI GCNGC 4 cut(s) 162, 297, 373, 567
Sau3AI GATC 7 cut(s) 49, 133, 312, 346, 487, 816, 894
Sau96I GGNCC 1 cut(s) 383
ScaI AGTACT 1 cut(s) 227
SchI GAGTC 1 cut(s) 707
ScrFI CCNGG 3 cut(s) 444, 609, 610
SduI GDGCHC 2 cut(s) 629, 651
SetI ASST 9 cut(s) 87, 183, 278, 399, 441, 651, 694, 706, 840
SfaNI GCATC 4 cut(s) 14, 31, 253, 889
Sfr274I CTCGAG 1 cut(s) 708
SinI GGWCC 1 cut(s) 383
SlaI CTCGAG 1 cut(s) 708
SmaI CCCGGG 1 cut(s) 610
SmlI CTYRAG 2 cut(s) 398, 708
SmoI CTYRAG 2 cut(s) 398, 708
Sse9I AATT 4 cut(s) 236, 635, 774, 831
SsiI CCGC 3 cut(s) 30, 162, 783
SspI AATATT 1 cut(s) 70
SspMI CTAG 1 cut(s) 752
SstI GAGCTC 1 cut(s) 651
StyD4I CCNGG 3 cut(s) 442, 607, 608
StyI CCWWGG 2 cut(s) 33, 307
TaaI ACNGT 2 cut(s) 552, 762
TaiI ACGT 2 cut(s) 278, 441
TaqI TCGA 5 cut(s) 149, 429, 709, 819, 897
TasI AATT 4 cut(s) 236, 635, 774, 831
TatI WGTACW 2 cut(s) 225, 573
TauI GCSGC 1 cut(s) 164
TfiI GAWTC 4 cut(s) 121, 127, 464, 534
Tru1I TTAA 2 cut(s) 81, 459
Tru9I TTAA 2 cut(s) 81, 459
TscAI CASTG 2 cut(s) 634, 724
TseI GCWGC 3 cut(s) 296, 372, 566
TspDTI ATGAA 3 cut(s) 581, 879, 921
TspMI CCCGGG 1 cut(s) 608
TspRI CASTG 2 cut(s) 634, 724
Tth111I GACNNNGTC 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 383
XapI RAATTY 2 cut(s) 236, 831
XhoI CTCGAG 1 cut(s) 708
XmaI CCCGGG 1 cut(s) 608
XspI CTAG 1 cut(s) 752
ZrmI AGTACT 1 cut(s) 227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.