pycom09g16060

Histone H3.3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0019551
Physical Location & Seq
Reverse (-)
16163775 .. 16164472
698 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGGCTCGTACGAAGCAAACTGCTCGCAAATCCACCGGCGGTAAGGCCCCGAGGAAGTCACTTGCCTTCAAGGCTGCAAGGAAATCAACACCCACAACCGGAGGAGTTAAGAAGCCTCACCGCTACCGCCCTGGAACCGTTGCTCTTCGGTTAACTTACAGTGAAATTCGAAAGTACCAGAAGAGCACTGAGCTTCTGATCCGCAAGCTGCCATTCCAACGCCTTGTTCGTGAACTCGCACAGGACTGCAAGACGGACCTCAGGTTCCAGAGCCATGCTATTCTTGCTCTCCAGGAAGCTGCGGAGGCTTATTTGGTTGGACTGTTTGAGGACACGAACCTTTGCGCCATTCATGCCAAAAGAGTTACCATAATGCCAAAGGACGTTCAGCTCGCTAGGAGGATCCGTGGTGAACGTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05202 ko05322 map05034 map05202 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

141

Amino Acids

15.86

Weight (kDa)

10.87

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 53 - 135 2.8e-35 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019551)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13370 AT1G75600
malus_domestica MD03G1194900.v1.1 MD09G1242000.v1.1
pyrus_communis pycom03g14700 pycom09g16060
rosa_laevigata RLG00000011176

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 39, 121, 127, 202, 302
AclWI GGATC 3 cut(s) 193, 397, 410
AcsI RAATTY 1 cut(s) 165
AfaI GTAC 2 cut(s) 10, 176
AfiI CCNNNNNNNGG 1 cut(s) 98
AgsI TTSAA 1 cut(s) 70
AjnI CCWGG 2 cut(s) 130, 291
AjuI GAANNNNNNNTTGG 2 cut(s) 210, 242
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 4 cut(s) 193, 208, 299, 391
AluI AGCT 4 cut(s) 193, 208, 299, 391
Alw21I GWGCWC 1 cut(s) 188
AlwI GGATC 3 cut(s) 193, 397, 410
Ama87I CYCGRG 1 cut(s) 49
AoxI GGCC 1 cut(s) 45
ApeKI GCWGC 3 cut(s) 74, 208, 299
ApoI RAATTY 1 cut(s) 165
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 46, 256
AsuHPI GGTGA 2 cut(s) 110, 422
AsuII TTCGAA 1 cut(s) 169
AvaI CYCGRG 1 cut(s) 49
AvaII GGWCC 1 cut(s) 256
AxyI CCTNAGG 1 cut(s) 260
BamHI GGATCC 1 cut(s) 402
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 61, 195, 286
BcgI CGANNNNNNTGC 2 cut(s) 5, 39
BciT130I CCWGG 2 cut(s) 132, 293
BfaI CTAG 1 cut(s) 396
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 3 cut(s) 75, 209, 300
BlsI GCNGC 3 cut(s) 76, 210, 301
Bme1390I CCNGG 2 cut(s) 132, 293
Bme18I GGWCC 1 cut(s) 256
BmeT110I CYCGRG 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 46, 256
BmiI GGNNCC 4 cut(s) 48, 136, 266, 404
BmrFI CCNGG 2 cut(s) 132, 293
BpmI CTGGAG 1 cut(s) 275
Bpu14I TTCGAA 1 cut(s) 169
BsaJI CCNNGG 3 cut(s) 50, 130, 406
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 1 cut(s) 98
Bse118I RCCGGY 1 cut(s) 35
Bse21I CCTNAGG 1 cut(s) 260
BseBI CCWGG 2 cut(s) 132, 293
BseDI CCNNGG 3 cut(s) 50, 130, 406
BseLI CCNNNNNNNGG 1 cut(s) 98
BseMII CTCAG 2 cut(s) 180, 274
BseRI GAGGAG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 61, 195, 286
BshFI GGCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 49
BsiSI CCGG 2 cut(s) 36, 99
BsiWI CGTACG 1 cut(s) 8
BslI CCNNNNNNNGG 1 cut(s) 98
BsnI GGCC 1 cut(s) 47
BsoBI CYCGRG 1 cut(s) 49
Bsp119I TTCGAA 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 2 cut(s) 198, 402
BspACI CCGC 5 cut(s) 39, 121, 127, 202, 302
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 2 cut(s) 181, 273
BspLI GGNNCC 4 cut(s) 48, 136, 266, 404
BspPI GGATC 3 cut(s) 193, 397, 410
BspQI GCTCTTC 2 cut(s) 150, 176
BspT104I TTCGAA 1 cut(s) 169
BsrFI RCCGGY 1 cut(s) 35
BssAI RCCGGY 1 cut(s) 35
BssECI CCNNGG 3 cut(s) 50, 130, 406
BssMI GATC 2 cut(s) 198, 402
Bst2UI CCWGG 2 cut(s) 132, 293
Bst4CI ACNGT 3 cut(s) 139, 161, 324
Bst6I CTCTTC 2 cut(s) 150, 176
BstBI TTCGAA 1 cut(s) 169
BstC8I GCNNGC 3 cut(s) 25, 206, 393
BstDEI CTNAG 2 cut(s) 189, 260
BstDSI CCRYGG 1 cut(s) 406
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 2 cut(s) 201, 405
BstMBI GATC 2 cut(s) 198, 402
BstMWI GCNNNNNNNGC 4 cut(s) 71, 284, 305, 353
BstNI CCWGG 2 cut(s) 132, 293
BstSCI CCNGG 2 cut(s) 130, 291
BstV1I GCAGC 3 cut(s) 61, 195, 286
BstX2I RGATCY 1 cut(s) 402
BstYI RGATCY 1 cut(s) 402
Bsu36I CCTNAGG 1 cut(s) 260
BsuRI GGCC 1 cut(s) 47
BtgI CCRYGG 1 cut(s) 406
BtsIMutI CAGTG 2 cut(s) 166, 186
Cac8I GCNNGC 3 cut(s) 25, 206, 393
CfoI GCGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 35
Cfr13I GGNCC 2 cut(s) 46, 256
Csp6I GTAC 2 cut(s) 9, 175
CviAII CATG 2 cut(s) 275, 353
CviQI GTAC 2 cut(s) 9, 175
DdeI CTNAG 2 cut(s) 189, 260
DpnI GATC 2 cut(s) 200, 404
DpnII GATC 2 cut(s) 198, 402
Eam1104I CTCTTC 2 cut(s) 150, 176
EarI CTCTTC 2 cut(s) 150, 176
Eco47I GGWCC 1 cut(s) 256
Eco81I CCTNAGG 1 cut(s) 260
Eco88I CYCGRG 1 cut(s) 49
EcoO109I RGGNCCY 1 cut(s) 46
EcoRII CCWGG 2 cut(s) 130, 291
FaeI CATG 2 cut(s) 278, 356
FaiI YATR 3 cut(s) 276, 354, 371
FatI CATG 2 cut(s) 274, 352
Fnu4HI GCNGC 3 cut(s) 75, 209, 300
Fsp4HI GCNGC 3 cut(s) 75, 209, 300
FspBI CTAG 1 cut(s) 396
GlaI GCGC 1 cut(s) 346
GluI GCNGC 3 cut(s) 75, 209, 300
GsuI CTGGAG 1 cut(s) 275
HaeIII GGCC 1 cut(s) 47
HapII CCGG 2 cut(s) 36, 99
HhaI GCGC 1 cut(s) 347
Hin1II CATG 2 cut(s) 278, 356
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HincII GTYRAC 1 cut(s) 153
HindII GTYRAC 1 cut(s) 153
HpaI GTTAAC 1 cut(s) 153
HpaII CCGG 2 cut(s) 36, 99
HphI GGTGA 2 cut(s) 110, 422
Hpy166II GTNNAC 3 cut(s) 153, 233, 413
Hpy188I TCNGA 1 cut(s) 198
Hpy188III TCNNGA 2 cut(s) 230, 268
Hpy8I GTNNAC 3 cut(s) 153, 233, 413
HpyAV CCTTC 1 cut(s) 76
HpyCH4III ACNGT 3 cut(s) 139, 161, 324
HpyCH4IV ACGT 2 cut(s) 384, 415
HpyCH4V TGCA 2 cut(s) 77, 249
HpyF10VI GCNNNNNNNGC 4 cut(s) 71, 284, 305, 353
HpyF3I CTNAG 2 cut(s) 189, 260
HpySE526I ACGT 2 cut(s) 384, 415
Hsp92II CATG 2 cut(s) 278, 356
HspAI GCGC 1 cut(s) 345
KspAI GTTAAC 1 cut(s) 153
Kzo9I GATC 2 cut(s) 198, 402
LguI GCTCTTC 2 cut(s) 150, 176
Lsp1109I GCAGC 3 cut(s) 61, 195, 286
MaeI CTAG 1 cut(s) 396
MaeII ACGT 2 cut(s) 384, 415
MaeIII GTNAC 2 cut(s) 57, 364
MalI GATC 2 cut(s) 200, 404
MboI GATC 2 cut(s) 198, 402
MboII GAAGA 2 cut(s) 137, 193
MflI RGATCY 1 cut(s) 402
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 1 cut(s) 165
MmeI TCCRAC 2 cut(s) 241, 298
MnlI CCTC 7 cut(s) 45, 95, 126, 269, 298, 322, 393
MseI TTAA 3 cut(s) 108, 152, 421
MspI CCGG 2 cut(s) 36, 99
MspR9I CCNGG 2 cut(s) 132, 293
MvaI CCWGG 2 cut(s) 132, 293
MwoI GCNNNNNNNGC 4 cut(s) 71, 284, 305, 353
NdeII GATC 2 cut(s) 198, 402
NlaIII CATG 2 cut(s) 278, 356
NlaIV GGNNCC 4 cut(s) 48, 136, 266, 404
NmuCI GTSAC 1 cut(s) 57
NspV TTCGAA 1 cut(s) 169
PciSI GCTCTTC 2 cut(s) 150, 176
PcsI WCGNNNNNNNCGW 1 cut(s) 226
Pfl23II CGTACG 1 cut(s) 8
PfoI TCCNGGA 1 cut(s) 291
PkrI GCNGC 3 cut(s) 76, 210, 301
Psp6I CCWGG 2 cut(s) 130, 291
PspGI CCWGG 2 cut(s) 130, 291
PspLI CGTACG 1 cut(s) 8
PspN4I GGNNCC 4 cut(s) 48, 136, 266, 404
PspPI GGNCC 2 cut(s) 46, 256
PsuI RGATCY 1 cut(s) 402
RsaI GTAC 2 cut(s) 10, 176
RsaNI GTAC 2 cut(s) 9, 175
SapI GCTCTTC 2 cut(s) 150, 176
SaqAI TTAA 3 cut(s) 108, 152, 421
SatI GCNGC 3 cut(s) 75, 209, 300
Sau3AI GATC 2 cut(s) 198, 402
Sau96I GGNCC 2 cut(s) 46, 256
ScrFI CCNGG 2 cut(s) 132, 293
SduI GDGCHC 1 cut(s) 188
SetI ASST 9 cut(s) 195, 210, 261, 266, 301, 342, 387, 393, 418
SfuI TTCGAA 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 35
SinI GGWCC 1 cut(s) 256
Sse9I AATT 1 cut(s) 165
SsiI CCGC 5 cut(s) 39, 121, 127, 202, 302
SspMI CTAG 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 130, 291
TaaI ACNGT 3 cut(s) 139, 161, 324
TaiI ACGT 2 cut(s) 387, 418
TaqI TCGA 1 cut(s) 169
TasI AATT 1 cut(s) 165
Tru1I TTAA 3 cut(s) 108, 152, 421
Tru9I TTAA 3 cut(s) 108, 152, 421
TscAI CASTG 2 cut(s) 166, 193
TseFI GTSAC 1 cut(s) 57
TseI GCWGC 3 cut(s) 74, 208, 299
Tsp45I GTSAC 1 cut(s) 57
TspDTI ATGAA 1 cut(s) 341
TspGWI ACGGA 2 cut(s) 269, 395
TspRI CASTG 2 cut(s) 166, 193
VpaK11BI GGWCC 1 cut(s) 256
XapI RAATTY 1 cut(s) 165
XspI CTAG 1 cut(s) 396
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.