pycom09g18720

Plastocyanin-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0021132
Physical Location & Seq
Reverse (-)
19497325 .. 19498792
1468 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGCATGCATGCATGCTGGTTTTTCAAGATCGCAAAATGCAGGCAATGAAGGCGATCATTGTGGTGGCGGTGATGCTCACATCAATGTTTTTCCGCTGCGGCGTCTCTGCTAGTACTGCGGCGATGGCTGCTGCAACCAACACCAACCACTCTGTTGGCGGAGCCTCCGGTTGGGATCTGTCCTCCAACCTCCGAGCATGGGCTACTCGCACCGCTTTCCGTGTGGGCGACTCTCTTGTATTCAGGTACACTCCGGTGCACGATGTAATGGAAGTGAGGAAGACGGACTATGATTTGTGTCACACAGTAGACCCACTGGAGATGCACAACGATGGGGAGACGGTGATTCCATTGAGGGAAGCAGGAAACAGGTACTTCATATGTGGGAGGCTGGGCCATTGTGCCATGGGACTCAAGCTCCATGTGCTTGTCCTCCCTGCACTGCAGATGAGTACCAATGTTAATGCAACCTCACCCAGCAGCCCCTTGATCACTAGTCCTCCGCCAAATGCTACTAGTCCTATTTCCAACAACAACAGCAGCAGACACGGTCCAGATGATCAAGTAGTGGGAAATGATCATGTGCAGCCTTCCCCATCGCCATCGCCATCTGCTTCCAGTTACTCAATTAATTCTACTGATGATGCACACTTAACTTCTGACAGCCCTCGGATTCTGCCATTGGTTACAGTGATCAGGCAGATCCTCGTGTTGGCCATTTTTTTATTTCCGAGTTGGATTGCCGCCTGGCCCTCCTCCATAGCTCCGTTTGTTCCGTTCACTCATGCTGCTCTGATTCTTCTCGTCGTTATTATCACTGTTGTTACTGTTGAGTTACTTCTCACTACTAGGCCAACTTCCAACAACAACGGCATACATGGTCCCGATAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0009506 GO:0016020 GO:0030054 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0055044 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

298

Amino Acids

32.04

Weight (kDa)

6.85

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 60 - 137 3.2e-24 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0021132)

Species Orthologous Gene IDs
malus_domestica MD06G1238600.v1.1 MD09G1277800.v1.1
prunus_persica Prupe.5G242500_v2.0.a1
pyrus_communis pycom06g21410 pycom09g18720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AciI CCGC 8 cut(s) 68, 94, 99, 119, 159, 213, 503, 744
AclWI GGATC 2 cut(s) 183, 697
AcoI YGGCCR 1 cut(s) 714
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 4 cut(s) 115, 248, 374, 454
AfiI CCNNNNNNNGG 3 cut(s) 171, 199, 712
AgsI TTSAA 1 cut(s) 26
AhlI ACTAGT 2 cut(s) 494, 515
AjnI CCWGG 1 cut(s) 746
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 2 cut(s) 418, 764
AluI AGCT 2 cut(s) 418, 764
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 109, 332
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 2 cut(s) 183, 697
AoxI GGCC 4 cut(s) 394, 714, 749, 851
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 7 cut(s) 96, 128, 131, 480, 540, 586, 788
AseI ATTAAT 1 cut(s) 630
AspS9I GGNCC 4 cut(s) 394, 551, 750, 881
AsuHPI GGTGA 3 cut(s) 82, 355, 465
AvaII GGWCC 2 cut(s) 551, 881
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 716
BauI CACGAG 1 cut(s) 707
BbsI GAAGAC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 7 cut(s) 83, 115, 118, 492, 552, 598, 775
BccI CCATC 5 cut(s) 118, 326, 604, 610, 616
BceAI ACGGC 1 cut(s) 886
BciT130I CCWGG 1 cut(s) 748
BclI TGATCA 4 cut(s) 489, 559, 577, 693
BcoDI GTCTC 2 cut(s) 109, 332
BcuI ACTAGT 2 cut(s) 494, 515
BfaI CTAG 4 cut(s) 111, 495, 516, 849
BfmI CTRYAG 1 cut(s) 443
BmcAI AGTACT 1 cut(s) 115
Bme1390I CCNGG 1 cut(s) 748
Bme18I GGWCC 2 cut(s) 551, 881
BmgT120I GGNCC 4 cut(s) 394, 551, 750, 881
BmiI GGNNCC 2 cut(s) 163, 883
BmrFI CCNGG 1 cut(s) 748
BmsI GCATC 3 cut(s) 63, 312, 634
BpiI GAAGAC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 398
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 2 cut(s) 405, 668
BsaWI WCCGGW 2 cut(s) 167, 253
BsaXI ACNNNNNCTCC 4 cut(s) 402, 432, 484, 514
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 199, 712
Bse1I ACTGG 2 cut(s) 321, 618
Bse3DI GCAATG 1 cut(s) 51
BseBI CCWGG 1 cut(s) 748
BseDI CCNNGG 2 cut(s) 405, 668
BseLI CCNNNNNNNGG 3 cut(s) 171, 199, 712
BseMI GCAATG 1 cut(s) 51
BseNI ACTGG 2 cut(s) 321, 618
BseRI GAGGAG 1 cut(s) 745
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 7 cut(s) 83, 115, 118, 492, 552, 598, 775
BseYI CCCAGC 2 cut(s) 391, 476
BsgI GTGCAG 2 cut(s) 423, 605
BshFI GGCC 4 cut(s) 396, 716, 751, 853
BsiHKAI GWGCWC 1 cut(s) 261
BsiSI CCGG 2 cut(s) 168, 254
BslFI GGGAC 2 cut(s) 423, 867
BslI CCNNNNNNNGG 3 cut(s) 171, 199, 712
BsmAI GTCTC 2 cut(s) 109, 332
BsmBI CGTCTC 2 cut(s) 109, 332
BsmFI GGGAC 2 cut(s) 423, 867
BsnI GGCC 4 cut(s) 396, 716, 751, 853
Bsp1286I GDGCHC 1 cut(s) 261
Bsp143I GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
Bsp19I CCATGG 1 cut(s) 405
BspACI CCGC 8 cut(s) 68, 94, 99, 119, 159, 213, 503, 744
BspANI GGCC 4 cut(s) 396, 716, 751, 853
BspLI GGNNCC 2 cut(s) 163, 883
BspMAI CTGCAG 1 cut(s) 447
BspPI GGATC 2 cut(s) 183, 697
BsrDI GCAATG 1 cut(s) 51
BsrI ACTGG 2 cut(s) 321, 618
BssECI CCNNGG 2 cut(s) 405, 668
BssMI GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
BssNI GRCGYC 1 cut(s) 102
BssSI CACGAG 1 cut(s) 707
BssT1I CCWWGG 1 cut(s) 405
Bst2BI CACGAG 1 cut(s) 707
Bst2UI CCWGG 1 cut(s) 748
Bst4CI ACNGT 6 cut(s) 307, 343, 551, 691, 820, 829
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 4 cut(s) 6, 10, 14, 42
BstDSI CCRYGG 1 cut(s) 405
BstKTI GATC 8 cut(s) 31, 57, 178, 492, 562, 580, 696, 705
BstMAI GTCTC 2 cut(s) 109, 332
BstMBI GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
BstMWI GCNNNNNNNGC 5 cut(s) 50, 116, 125, 128, 424
BstNI CCWGG 1 cut(s) 748
BstNSI RCATGY 3 cut(s) 8, 12, 16
BstSCI CCNGG 1 cut(s) 746
BstSFI CTRYAG 1 cut(s) 443
BstSLI GKGCMC 1 cut(s) 261
BstV1I GCAGC 7 cut(s) 83, 115, 118, 492, 552, 598, 775
BstV2I GAAGAC 1 cut(s) 287
BstX2I RGATCY 2 cut(s) 175, 702
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 2 cut(s) 175, 702
BsuRI GGCC 4 cut(s) 396, 716, 751, 853
BtgI CCRYGG 1 cut(s) 405
BtgZI GCGATG 3 cut(s) 137, 582, 588
BtsI GCAGTG 1 cut(s) 440
BtsIMutI CAGTG 4 cut(s) 314, 440, 696, 816
Cac8I GCNNGC 4 cut(s) 6, 10, 14, 42
Cfr13I GGNCC 4 cut(s) 394, 551, 750, 881
CseI GACGC 1 cut(s) 91
Csp6I GTAC 4 cut(s) 114, 247, 373, 453
CviAII CATG 9 cut(s) 5, 9, 13, 198, 406, 422, 581, 785, 878
CviQI GTAC 4 cut(s) 114, 247, 373, 453
DpnI GATC 8 cut(s) 30, 56, 177, 491, 561, 579, 695, 704
DpnII GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
EaeI YGGCCR 1 cut(s) 714
EciI GGCGGA 2 cut(s) 174, 492
Eco130I CCWWGG 1 cut(s) 405
Eco47I GGWCC 2 cut(s) 551, 881
EcoRII CCWGG 1 cut(s) 746
EcoT14I CCWWGG 1 cut(s) 405
EcoT22I ATGCAT 3 cut(s) 6, 10, 14
ErhI CCWWGG 1 cut(s) 405
Esp3I CGTCTC 2 cut(s) 109, 332
FaeI CATG 9 cut(s) 8, 12, 16, 201, 409, 425, 584, 788, 881
FaqI GGGAC 2 cut(s) 423, 867
FatI CATG 9 cut(s) 4, 8, 12, 197, 405, 421, 580, 784, 877
FauNDI CATATG 1 cut(s) 380
FbaI TGATCA 4 cut(s) 489, 559, 577, 693
FblI GTMKAC 1 cut(s) 309
FspBI CTAG 4 cut(s) 111, 495, 516, 849
GsaI CCCAGC 2 cut(s) 395, 480
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 4 cut(s) 396, 716, 751, 853
HapII CCGG 2 cut(s) 168, 254
HgaI GACGC 1 cut(s) 91
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 9 cut(s) 8, 12, 16, 201, 409, 425, 584, 788, 881
HinfI GANTC 5 cut(s) 230, 346, 411, 673, 796
HpaII CCGG 2 cut(s) 168, 254
HphI GGTGA 3 cut(s) 82, 355, 465
Hpy166II GTNNAC 4 cut(s) 249, 259, 310, 780
Hpy188I TCNGA 5 cut(s) 194, 661, 672, 732, 795
Hpy188III TCNNGA 3 cut(s) 26, 554, 884
Hpy8I GTNNAC 4 cut(s) 249, 259, 310, 780
Hpy99I CGWCG 1 cut(s) 809
HpyAV CCTTC 2 cut(s) 43, 600
HpyCH4III ACNGT 6 cut(s) 307, 343, 551, 691, 820, 829
HpyF10VI GCNNNNNNNGC 5 cut(s) 50, 116, 125, 128, 424
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 9 cut(s) 8, 12, 16, 201, 409, 425, 584, 788, 881
Ksp22I TGATCA 4 cut(s) 489, 559, 577, 693
Kzo9I GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
LmnI GCTCC 3 cut(s) 161, 423, 769
Lsp1109I GCAGC 7 cut(s) 83, 115, 118, 492, 552, 598, 775
LweI GCATC 3 cut(s) 63, 312, 634
MaeI CTAG 4 cut(s) 111, 495, 516, 849
MaeIII GTNAC 5 cut(s) 299, 620, 685, 823, 834
MalI GATC 8 cut(s) 30, 56, 177, 491, 561, 579, 695, 704
MboI GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
MboII GAAGA 2 cut(s) 292, 791
MflI RGATCY 2 cut(s) 175, 702
MhlI GDGCHC 1 cut(s) 261
MlsI TGGCCA 1 cut(s) 716
MluCI AATT 2 cut(s) 627, 631
MluNI TGGCCA 1 cut(s) 716
MlyI GAGTC 2 cut(s) 224, 405
MmeI TCCRAC 4 cut(s) 210, 552, 716, 885
Mox20I TGGCCA 1 cut(s) 716
Mph1103I ATGCAT 3 cut(s) 6, 10, 14
MscI TGGCCA 1 cut(s) 716
MseI TTAA 3 cut(s) 462, 630, 653
MslI CAYNNNNRTG 4 cut(s) 62, 83, 254, 330
Msp20I TGGCCA 1 cut(s) 716
MspA1I CMGCKG 1 cut(s) 96
MspI CCGG 2 cut(s) 168, 254
MspR9I CCNGG 1 cut(s) 748
MvaI CCWGG 1 cut(s) 748
MwoI GCNNNNNNNGC 5 cut(s) 50, 116, 125, 128, 424
NcoI CCATGG 1 cut(s) 405
NdeI CATATG 1 cut(s) 380
NdeII GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
NlaIII CATG 9 cut(s) 8, 12, 16, 201, 409, 425, 584, 788, 881
NlaIV GGNNCC 2 cut(s) 163, 883
NmuCI GTSAC 1 cut(s) 299
NsiI ATGCAT 3 cut(s) 6, 10, 14
NspI RCATGY 3 cut(s) 8, 12, 16
OliI CACNNNNGTG 1 cut(s) 254
PaeI GCATGC 3 cut(s) 8, 12, 16
PfeI GAWTC 3 cut(s) 346, 673, 796
PflFI GACNNNGTC 1 cut(s) 549
PleI GAGTC 2 cut(s) 224, 405
PpsI GAGTC 2 cut(s) 224, 405
PshBI ATTAAT 1 cut(s) 630
Psp6I CCWGG 1 cut(s) 746
PspFI CCCAGC 2 cut(s) 391, 476
PspGI CCWGG 1 cut(s) 746
PspN4I GGNNCC 2 cut(s) 163, 883
PspPI GGNCC 4 cut(s) 394, 551, 750, 881
PstI CTGCAG 1 cut(s) 447
PsuI RGATCY 2 cut(s) 175, 702
PsyI GACNNNGTC 1 cut(s) 549
RsaI GTAC 4 cut(s) 115, 248, 374, 454
RsaNI GTAC 4 cut(s) 114, 247, 373, 453
RseI CAYNNNNRTG 4 cut(s) 62, 83, 254, 330
SaqAI TTAA 3 cut(s) 462, 630, 653
Sau3AI GATC 8 cut(s) 28, 54, 175, 489, 559, 577, 693, 702
Sau96I GGNCC 4 cut(s) 394, 551, 750, 881
ScaI AGTACT 1 cut(s) 115
SchI GAGTC 2 cut(s) 224, 405
ScrFI CCNGG 1 cut(s) 748
SduI GDGCHC 1 cut(s) 261
SetI ASST 6 cut(s) 192, 248, 374, 420, 473, 766
SfaNI GCATC 3 cut(s) 63, 312, 634
SfcI CTRYAG 1 cut(s) 443
SinI GGWCC 2 cut(s) 551, 881
SmiMI CAYNNNNRTG 4 cut(s) 62, 83, 254, 330
SmlI CTYRAG 1 cut(s) 413
SmoI CTYRAG 1 cut(s) 413
SpeI ACTAGT 2 cut(s) 494, 515
SphI GCATGC 3 cut(s) 8, 12, 16
Sse9I AATT 2 cut(s) 627, 631
SsiI CCGC 8 cut(s) 68, 94, 99, 119, 159, 213, 503, 744
SspMI CTAG 4 cut(s) 111, 495, 516, 849
StyD4I CCNGG 1 cut(s) 746
StyI CCWWGG 1 cut(s) 405
TaaI ACNGT 6 cut(s) 307, 343, 551, 691, 820, 829
TasI AATT 2 cut(s) 627, 631
TatI WGTACW 1 cut(s) 113
TauI GCSGC 3 cut(s) 102, 122, 746
TfiI GAWTC 3 cut(s) 346, 673, 796
Tru1I TTAA 3 cut(s) 462, 630, 653
Tru9I TTAA 3 cut(s) 462, 630, 653
TscAI CASTG 4 cut(s) 321, 447, 696, 823
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 7 cut(s) 96, 128, 131, 480, 540, 586, 788
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 2 cut(s) 62, 367
TspGWI ACGGA 4 cut(s) 209, 299, 756, 765
TspRI CASTG 4 cut(s) 321, 447, 696, 823
Tth111I GACNNNGTC 1 cut(s) 549
VneI GTGCAC 1 cut(s) 257
VpaK11BI GGWCC 2 cut(s) 551, 881
VspI ATTAAT 1 cut(s) 630
XceI RCATGY 3 cut(s) 8, 12, 16
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 4 cut(s) 111, 495, 516, 849
ZrmI AGTACT 1 cut(s) 115
Zsp2I ATGCAT 3 cut(s) 6, 10, 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.