pycom10g03930

callose synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0022292
Physical Location & Seq
Forward (+)
4210242 .. 4211989
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1266 bp
ATGAGAAAATTAGAAAAATGTAGGAAAGCATTTGGCATGGGGTTTAAGGAAATCCAGTGTCGCGTCCAGTTTGAGACTTTGGCTACGGCAGATAAGCCACTGAAGGTAGCACTACATCTTCATGTGCCCCACCCATTAACCAACCAATGTGTTCCCTCGGCGAGATGGCACAAACTAACAAACACAATCGCGCATCGTCATCTGGGCGTATCTACAATTCCAAATCTAAATTACCCACCAGTAGGTAACAGATTCTTTATGTGCCAATGTTGTGTACTTTTTATAAGGTTTTTTTCACAAATAACATTTTACTGTATTCATATTAAAGGGAAAATAAGAGCCTCTCTAATGAAGGCTTTATCCTCCTTTTCTTTTAGGGCTGATATAAATGTAGCACTAGTGAGAAATTTCATCCCAATGAACAGGAAAACGGGTCTACATCCACCACATGGGCTAGTATCTTCCATGAATAATCCACATCAAATTGAGGGCCAAGTCTTGGTTTGTTTATTTTATTTTATTATTATTATTAGTTTAGAAATTGGTAGGCTCCATTTTTTATTCCAAAACACCATTTTAAAGGTTTATTTTTCCCCCCCTCCCAAAAAAAATCCACTCCAACCTAAAACCCCACAAATTGAAGTGGGACCCACGGATGAGAGTGAAAAGTGGGCTGTGAAGCCTACACCCCAAAACCCAGTCTCCCAACGTGTTGCACGCACCATAGACTTTTCTAGCCGAGGCTCACCCACGTGGGGCTGTCCCCTTTCCAACGGTCCACGTTCAAATTTTTTTATTTTTTATGTTTATATATTTATTGAATCCAACGGCTTAGATCAAATACAATCAAATCTAATGGTAAAAAAAAGATCTAACGTTAACTTAAAATTTAACCAAAAACTCTATAAATACCTATGCATTTGTTCAAACATCCACACAAAACTCACTTTTCTCCTACAATTCTTCCAATTTTTCTTTCTACCATTTCTTCTCATTTCCAAAATTTTCAAGATGGCAAAAGAGCATGTTAGAGGTCGTAATTGGACCTTTGATGAAGATATTGCTTTATCTTTGGCATGGATTTCTAGTAGTGAAGATGGTGCCATCATGATAAGTTCCATGAAAACTCCAATGCCGGTCAAAGGGAAGTTGGTGGTGTTTATGATCGGTGGAACATTATCAACAAAGTGTGCACTTTGTGGAAGGGAAGCTTGGAGAGAGCCATGGTTGACATGCCTAGTGGATGGGGCGCCTCAGAAATTGTGA

Protein Analysis

422

Amino Acids

48.15

Weight (kDa)

9.83

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0022292)

Species Orthologous Gene IDs
malus_domestica MD04G1113800.v1.1 MD06G1057800.v1.1 MD10G1107400.v1.1
pyrus_communis pycom10g03930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
AccB1I GGYRCC 2 cut(s) 1100, 1249
AccB7I CCANNNNNTGG 2 cut(s) 449, 499
AccI GTMKAC 1 cut(s) 436
AccII CGCG 2 cut(s) 63, 191
AclI AACGTT 1 cut(s) 876
AcsI RAATTY 4 cut(s) 406, 787, 887, 1002
AcuI CTGAAG 1 cut(s) 122
AcvI CACGTG 1 cut(s) 753
AcyI GRCGYC 1 cut(s) 1250
AdeI CACNNNGTG 1 cut(s) 1199
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 5 cut(s) 242, 449, 499, 755, 1142
AflIII ACRYGT 1 cut(s) 709
AgsI TTSAA 5 cut(s) 641, 786, 821, 927, 1009
AhlI ACTAGT 1 cut(s) 397
AjuI GAANNNNNNNTTGG 4 cut(s) 960, 992, 1195, 1227
AleI CACNNNNGTG 1 cut(s) 751
AluBI AGCT 1 cut(s) 1211
AluI AGCT 1 cut(s) 1211
Alw21I GWGCWC 1 cut(s) 1195
Alw26I GTCTC 2 cut(s) 68, 706
Alw44I GTGCAC 1 cut(s) 1191
AoxI GGCC 1 cut(s) 490
ApaLI GTGCAC 1 cut(s) 1191
ApoI RAATTY 4 cut(s) 406, 787, 887, 1002
AspLEI GCGC 2 cut(s) 193, 1252
AspS9I GGNCC 4 cut(s) 490, 647, 776, 1044
AsuHPI GGTGA 1 cut(s) 738
AvaII GGWCC 3 cut(s) 647, 776, 1044
BaeGI GKGCMC 2 cut(s) 129, 1195
BanI GGYRCC 2 cut(s) 1100, 1249
BbrPI CACGTG 1 cut(s) 753
Bbv12I GWGCWC 1 cut(s) 1195
BccI CCATC 5 cut(s) 159, 1006, 1091, 1112, 1238
BceAI ACGGC 2 cut(s) 102, 844
BcoDI GTCTC 2 cut(s) 68, 706
BcuI ACTAGT 1 cut(s) 397
BfaI CTAG 5 cut(s) 398, 455, 735, 1086, 1238
BfoI RGCGCY 1 cut(s) 1253
BglII AGATCT 1 cut(s) 869
Bme18I GGWCC 3 cut(s) 647, 776, 1044
BmgT120I GGNCC 4 cut(s) 490, 647, 776, 1044
BmiI GGNNCC 5 cut(s) 551, 648, 649, 1102, 1251
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 1 cut(s) 202
BmuI ACTGGG 1 cut(s) 692
BsaAI YACGTR 1 cut(s) 753
BsaHI GRCGYC 1 cut(s) 1250
BsaJI CCNNGG 4 cut(s) 156, 651, 739, 1223
BsaXI ACNNNNNCTCC 2 cut(s) 686, 716
Bsc4I CCNNNNNNNGG 5 cut(s) 242, 449, 499, 755, 1142
Bse118I RCCGGY 1 cut(s) 1135
Bse1I ACTGG 4 cut(s) 55, 67, 239, 698
BseDI CCNNGG 4 cut(s) 156, 651, 739, 1223
BseGI GGATG 5 cut(s) 411, 439, 661, 930, 1249
BseLI CCNNNNNNNGG 5 cut(s) 242, 449, 499, 755, 1142
BseNI ACTGG 4 cut(s) 55, 67, 239, 698
BseSI GKGCMC 2 cut(s) 129, 1195
Bsh1236I CGCG 2 cut(s) 63, 191
BshFI GGCC 1 cut(s) 492
BshNI GGYRCC 2 cut(s) 1100, 1249
BsiHKAI GWGCWC 1 cut(s) 1195
BsiSI CCGG 1 cut(s) 1136
BslFI GGGAC 2 cut(s) 660, 747
BslI CCNNNNNNNGG 5 cut(s) 242, 449, 499, 755, 1142
BsmAI GTCTC 2 cut(s) 68, 706
BsmFI GGGAC 2 cut(s) 660, 747
BsnI GGCC 1 cut(s) 492
Bsp1286I GDGCHC 2 cut(s) 129, 1195
Bsp143I GATC 3 cut(s) 835, 869, 1164
Bsp19I CCATGG 1 cut(s) 1223
BspANI GGCC 1 cut(s) 492
BspFNI CGCG 2 cut(s) 63, 191
BspHI TCATGA 1 cut(s) 1107
BspLI GGNNCC 5 cut(s) 551, 648, 649, 1102, 1251
BspT107I GGYRCC 2 cut(s) 1100, 1249
BsrFI RCCGGY 1 cut(s) 1135
BsrI ACTGG 4 cut(s) 55, 67, 239, 698
BssAI RCCGGY 1 cut(s) 1135
BssECI CCNNGG 4 cut(s) 156, 651, 739, 1223
BssMI GATC 3 cut(s) 835, 869, 1164
BssNI GRCGYC 1 cut(s) 1250
BssT1I CCWWGG 1 cut(s) 1223
Bst4CI ACNGT 2 cut(s) 314, 776
BstACI GRCGYC 1 cut(s) 1250
BstBAI YACGTR 1 cut(s) 753
BstC8I GCNNGC 1 cut(s) 718
BstDEI CTNAG 2 cut(s) 832, 1254
BstDSI CCRYGG 2 cut(s) 651, 1223
BstF5I GGATG 5 cut(s) 411, 439, 661, 930, 1249
BstFNI CGCG 2 cut(s) 63, 191
BstH2I RGCGCY 1 cut(s) 1253
BstHHI GCGC 2 cut(s) 193, 1252
BstKTI GATC 3 cut(s) 838, 872, 1167
BstMAI GTCTC 2 cut(s) 68, 706
BstMBI GATC 3 cut(s) 835, 869, 1164
BstNSI RCATGY 2 cut(s) 1028, 1236
BstSLI GKGCMC 2 cut(s) 129, 1195
BstUI CGCG 2 cut(s) 63, 191
BstX2I RGATCY 1 cut(s) 869
BstYI RGATCY 1 cut(s) 869
BsuRI GGCC 1 cut(s) 492
BtgI CCRYGG 2 cut(s) 651, 1223
BtsCI GGATG 5 cut(s) 411, 439, 661, 930, 1249
BtsIMutI CAGTG 2 cut(s) 62, 98
Cac8I GCNNGC 1 cut(s) 718
CciI TCATGA 1 cut(s) 1107
CfoI GCGC 2 cut(s) 193, 1252
Cfr10I RCCGGY 1 cut(s) 1135
Cfr13I GGNCC 4 cut(s) 490, 647, 776, 1044
CseI GACGC 1 cut(s) 52
Csp6I GTAC 1 cut(s) 275
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 2 cut(s) 832, 1254
DinI GGCGCC 1 cut(s) 1251
DpnI GATC 3 cut(s) 837, 871, 1166
DpnII GATC 3 cut(s) 835, 869, 1164
DraI TTTAAA 1 cut(s) 579
DraIII CACNNNGTG 1 cut(s) 1199
Eco130I CCWWGG 1 cut(s) 1223
Eco47I GGWCC 3 cut(s) 647, 776, 1044
Eco57I CTGAAG 1 cut(s) 122
Eco72I CACGTG 1 cut(s) 753
EcoO109I RGGNCCY 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 1223
EcoT22I ATGCAT 1 cut(s) 920
EgeI GGCGCC 1 cut(s) 1251
EheI GGCGCC 1 cut(s) 1251
ErhI CCWWGG 1 cut(s) 1223
FalI AAGNNNNNCTT 2 cut(s) 1195, 1227
FaqI GGGAC 2 cut(s) 660, 747
FblI GTMKAC 1 cut(s) 436
FokI GGATG 5 cut(s) 398, 426, 668, 917, 1256
FspBI CTAG 5 cut(s) 398, 455, 735, 1086, 1238
GlaI GCGC 2 cut(s) 192, 1251
HaeII RGCGCY 1 cut(s) 1253
HaeIII GGCC 1 cut(s) 492
HapII CCGG 1 cut(s) 1136
HgaI GACGC 1 cut(s) 52
HhaI GCGC 2 cut(s) 193, 1252
Hin1I GRCGYC 1 cut(s) 1250
Hin6I GCGC 2 cut(s) 191, 1250
HinP1I GCGC 2 cut(s) 191, 1250
HincII GTYRAC 2 cut(s) 880, 1230
HindII GTYRAC 2 cut(s) 880, 1230
HindIII AAGCTT 1 cut(s) 1209
HinfI GANTC 2 cut(s) 252, 821
HpaI GTTAAC 1 cut(s) 880
HpaII CCGG 1 cut(s) 1136
HphI GGTGA 1 cut(s) 738
Hpy166II GTNNAC 6 cut(s) 275, 437, 779, 880, 1193, 1230
Hpy188I TCNGA 1 cut(s) 1257
Hpy188III TCNNGA 2 cut(s) 1009, 1108
Hpy8I GTNNAC 6 cut(s) 275, 437, 779, 880, 1193, 1230
HpyAV CCTTC 3 cut(s) 97, 346, 1197
HpyCH4III ACNGT 2 cut(s) 314, 776
HpyCH4IV ACGT 4 cut(s) 709, 752, 781, 876
HpyCH4V TGCA 3 cut(s) 716, 918, 1193
HpyF3I CTNAG 2 cut(s) 832, 1254
HpySE526I ACGT 4 cut(s) 709, 752, 781, 876
Hsp92I GRCGYC 1 cut(s) 1250
HspAI GCGC 2 cut(s) 191, 1250
KasI GGCGCC 1 cut(s) 1249
KflI GGGWCCC 1 cut(s) 647
KspAI GTTAAC 1 cut(s) 880
Kzo9I GATC 3 cut(s) 835, 869, 1164
LmnI GCTCC 1 cut(s) 555
LpnPI CCDG 7 cut(s) 68, 80, 188, 252, 409, 711, 1149
LweI GCATC 1 cut(s) 202
MaeI CTAG 5 cut(s) 398, 455, 735, 1086, 1238
MaeII ACGT 4 cut(s) 709, 752, 781, 876
MaeIII GTNAC 1 cut(s) 245
MalI GATC 3 cut(s) 837, 871, 1166
MboI GATC 3 cut(s) 835, 869, 1164
MboII GAAGA 6 cut(s) 110, 453, 955, 980, 1067, 1106
MflI RGATCY 1 cut(s) 869
MhlI GDGCHC 2 cut(s) 129, 1195
Mly113I GGCGCC 1 cut(s) 1250
MmeI TCCRAC 3 cut(s) 643, 795, 849
MnlI CCTC 8 cut(s) 166, 352, 373, 481, 609, 734, 1025, 1263
Mph1103I ATGCAT 1 cut(s) 920
MseI TTAA 7 cut(s) 45, 137, 324, 578, 879, 884, 891
MslI CAYNNNNRTG 3 cut(s) 120, 416, 751
MspI CCGG 1 cut(s) 1136
MvnI CGCG 2 cut(s) 63, 191
NarI GGCGCC 1 cut(s) 1250
NcoI CCATGG 1 cut(s) 1223
NdeII GATC 3 cut(s) 835, 869, 1164
NlaIV GGNNCC 5 cut(s) 551, 648, 649, 1102, 1251
NmeAIII GCCGAG 2 cut(s) 137, 764
NsiI ATGCAT 1 cut(s) 920
NspI RCATGY 2 cut(s) 1028, 1236
OliI CACNNNNGTG 1 cut(s) 751
PagI TCATGA 1 cut(s) 1107
PfeI GAWTC 2 cut(s) 252, 821
PflMI CCANNNNNTGG 2 cut(s) 449, 499
PluTI GGCGCC 1 cut(s) 1253
PmaCI CACGTG 1 cut(s) 753
PmlI CACGTG 1 cut(s) 753
Ppu21I YACGTR 1 cut(s) 753
PpuMI RGGWCCY 1 cut(s) 647
PsiI TTATAA 1 cut(s) 284
Psp1406I AACGTT 1 cut(s) 876
Psp5II RGGWCCY 1 cut(s) 647
PspCI CACGTG 1 cut(s) 753
PspN4I GGNNCC 5 cut(s) 551, 648, 649, 1102, 1251
PspPI GGNCC 4 cut(s) 490, 647, 776, 1044
PspPPI RGGWCCY 1 cut(s) 647
PsuI RGATCY 1 cut(s) 869
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 3 cut(s) 120, 416, 751
SaqAI TTAA 7 cut(s) 45, 137, 324, 578, 879, 884, 891
Sau3AI GATC 3 cut(s) 835, 869, 1164
Sau96I GGNCC 4 cut(s) 490, 647, 776, 1044
SduI GDGCHC 2 cut(s) 129, 1195
SfaNI GCATC 1 cut(s) 202
SfoI GGCGCC 1 cut(s) 1251
SinI GGWCC 3 cut(s) 647, 776, 1044
SmiMI CAYNNNNRTG 3 cut(s) 120, 416, 751
SpeI ACTAGT 1 cut(s) 397
SspDI GGCGCC 1 cut(s) 1249
SspMI CTAG 5 cut(s) 398, 455, 735, 1086, 1238
StyI CCWWGG 1 cut(s) 1223
TaaI ACNGT 2 cut(s) 314, 776
TaiI ACGT 4 cut(s) 712, 755, 784, 879
TatI WGTACW 1 cut(s) 274
TfiI GAWTC 2 cut(s) 252, 821
Tru1I TTAA 7 cut(s) 45, 137, 324, 578, 879, 884, 891
Tru9I TTAA 7 cut(s) 45, 137, 324, 578, 879, 884, 891
TscAI CASTG 2 cut(s) 62, 105
TspDTI ATGAA 8 cut(s) 110, 308, 365, 400, 434, 482, 1068, 1136
TspGWI ACGGA 1 cut(s) 668
TspRI CASTG 2 cut(s) 62, 105
Van91I CCANNNNNTGG 2 cut(s) 449, 499
VneI GTGCAC 1 cut(s) 1191
VpaK11BI GGWCC 3 cut(s) 647, 776, 1044
XapI RAATTY 4 cut(s) 406, 787, 887, 1002
XceI RCATGY 2 cut(s) 1028, 1236
XmiI GTMKAC 1 cut(s) 436
XspI CTAG 5 cut(s) 398, 455, 735, 1086, 1238
Zsp2I ATGCAT 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.