pycom10g16620

Phospholipase A1-Igamma2, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009430
Physical Location & Seq
Reverse (-)
19797753 .. 19799691
1939 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1644 bp
ATGCGCAGTGGAAGAGCAGAGAAGCCAGCTCCCTTTCTTTTACATACAAAACATTGCTCTCTGCGTCATTCACCACTGAGTTGCTCACCACCTCTTTCATCGTCGTCTCCAATGGCAGCCATTTCTTTATCCTGCACCGTTCTTCCCTTCACAAACCTCAACTTATCAGCCTACAAATCACACCCCTTTGGAACCTCGAAACTCACCGTTTCAACCTCCACCGTCGCCGCCAAAGTCCTCCACTCAAAAACCACCGATTCCTTACCCTCCATCATCACCGAGCTTGAAAAAGAAAGAGTCCACAGCAAAGAAGAGAACGACCAAAATGAACGAGAGACCGACACTACCACTCACGAAAACGAGCGTCGTTTGTCCGATGTTTGGCGGGAACTCCACGGTCAAGATGACTGGGTCGGGTTACTCGACCCGATAGACCCCCTCCTCCGATCTGAGCTCATCCGCTATGGCGAGATGGCCCAGGCCTGCTACGACGCCTTCGACTTCGATCCCTTCTCCAAGTACTGTGGCAGCTGCCGCTTCACGCGCCACGCCTTCTTCGAGTCCCTGGGCATGGCCCAGAACGGCTACCACGTGTCGCGCTACCTCTTCGCCACGTCCAATATCAACCTTCCAAACTTCTTCAAGAAGTCCCGCTGGCCCAAGGTATGGAGCAAAAATGCCAACTGGATTGGATATGTCGCCGTTTCGGACGACGATACGTCGGCGCGTTTGGGCCGTCGAGACATCAGCATTGCGTGGCGCGGCACCGTCACCCGCCTCGAGTGGATCGTCGACTTAATGGACTTTCTAAAGCCGGTTTCCGCGAATCGAATCCCGTGCCCGGATCAGACTGTGAAAGTCGAATCCGGTTTTCTGGATCTCTACACCGATAAAGACGTGACGTGCCGATTTTGCCATTACTCGGCGAGGGAGCAGATTTTGACGGAGATTAAACGGTTGGTGGAAAAATACTCGGACGAGGAATTGAGCATCACGATTACCGGGCACAGCCTCGGGAGCGCGTTGGCAATCTTGAGCGCGTACGACATAACCGAAACGGGTTTGAACGTGATGTCGGACGGGCGGGTCGTGCCCGTATCCGTATTGTCGTTTTCGGGTCCTCGGGTCGGGAACGTGCGGTTTAAGGAGCGGCTCGAGTCGTTGGGTGTTAAAGTGTTGAGAGTGGTGAACGTTCACGATGTGGTCCCCAAGTCGCCGGGGCTGTTCTTTAACGAACACGTGGCGCCGAGGGTCATGAAATTAGCGGAGGGTCTGCCGTGGAGCTATTCGCATGTCGGAGTGGAACTTAAGCTGGATCACAAAAACTCGCCCTTTTTGAAGCCGACGAATGACCCTGGTTGCGCCCATAATTTGGAGGCTCACTTGCATTTGCTGGATGGGTACCATGGAAAAGGCCACAGATTTGTGCTAGCCAGTGGAAGAGACCCAGCACTGGTGAACAAAGCCTCGGATTTTTTGAAAGACCATTACTTGGTTCCACCTTACTGGAGGCAAGATCGATCGCAACAAGGGCATGGTGAGGTGCAAGGACGGCCGGTGGATCCAGGCCGAACGGCCGAAACTCGACGATCATCACCCCGAAGACATACACCACCATCTCAAGCAACTAGGCCTTGCTTCTGA

Protein Analysis

548

Amino Acids

61.56

Weight (kDa)

8.52

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_3 PF01764 251 - 408 7.6e-37 Lipase (class 3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1085
Acc16I TGCGCA 1 cut(s) 5
Acc65I GGTACC 1 cut(s) 1401
AccB1I GGYRCC 3 cut(s) 764, 1243, 1401
AccB7I CCANNNNNTGG 3 cut(s) 666, 1372, 1492
AccBSI CCGCTC 1 cut(s) 1150
AccI GTMKAC 1 cut(s) 792
AccII CGCG 7 cut(s) 544, 598, 727, 762, 824, 1022, 1040
AclI AACGTT 1 cut(s) 1191
AclWI GGATC 7 cut(s) 500, 794, 852, 885, 1323, 1556, 1569
AcoI YGGCCR 2 cut(s) 1553, 1575
AcvI CACGTG 2 cut(s) 592, 1240
AcyI GRCGYC 2 cut(s) 492, 1244
AdeI CACNNNGTG 1 cut(s) 1201
AfaI GTAC 3 cut(s) 521, 1043, 1403
AfiI CCNNNNNNNGG 9 cut(s) 381, 571, 666, 841, 922, 1127, 1372, 1453, 1492
AflII CTTAAG 1 cut(s) 1307
AflIII ACRYGT 2 cut(s) 591, 1237
AgsI TTSAA 6 cut(s) 213, 287, 643, 1066, 1339, 1480
AhdI GACNNNNNGTC 1 cut(s) 718
AjiI CACGTC 3 cut(s) 615, 898, 903
AjnI CCWGG 4 cut(s) 477, 564, 1354, 1564
AluBI AGCT 6 cut(s) 29, 283, 454, 531, 1284, 1312
AluI AGCT 6 cut(s) 29, 283, 454, 531, 1284, 1312
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 4 cut(s) 111, 329, 735, 1437
AlwI GGATC 7 cut(s) 500, 794, 852, 885, 1323, 1556, 1569
Ama87I CYCGRG 4 cut(s) 779, 1013, 1122, 1154
ApeKI GCWGC 3 cut(s) 116, 528, 531
Asp718I GGTACC 1 cut(s) 1401
AspLEI GCGC 9 cut(s) 6, 546, 600, 727, 762, 1022, 1040, 1246, 1364
AspS9I GGNCC 6 cut(s) 475, 574, 657, 733, 1118, 1204
AsuC2I CCSGG 3 cut(s) 842, 1003, 1218
AsuHPI GGTGA 9 cut(s) 63, 78, 196, 268, 763, 1198, 1468, 1550, 1587
AsuNHI GCTAGC 1 cut(s) 1429
AvaI CYCGRG 4 cut(s) 779, 1013, 1122, 1154
AvaII GGWCC 2 cut(s) 1118, 1204
BaeGI GKGCMC 3 cut(s) 842, 1008, 1095
BamHI GGATCC 1 cut(s) 1561
BanI GGYRCC 3 cut(s) 764, 1243, 1401
BanII GRGCYC 1 cut(s) 456
BbrPI CACGTG 2 cut(s) 592, 1240
BbsI GAAGAC 1 cut(s) 1609
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 3 cut(s) 128, 518, 540
BccI CCATC 4 cut(s) 278, 466, 1391, 1624
BceAI ACGGC 6 cut(s) 598, 686, 720, 1261, 1568, 1590
BcgI CGANNNNNNTGC 2 cut(s) 819, 853
BciT130I CCWGG 4 cut(s) 479, 566, 1356, 1566
BciVI GTATCC 1 cut(s) 1108
BcnI CCSGG 3 cut(s) 842, 1003, 1218
BcoDI GTCTC 4 cut(s) 111, 329, 735, 1437
BfaI CTAG 2 cut(s) 1430, 1629
BfoI RGCGCY 1 cut(s) 1247
BfrI CTTAAG 1 cut(s) 1307
BfuI GTATCC 1 cut(s) 1108
BisI GCNGC 7 cut(s) 117, 228, 529, 532, 535, 763, 1151
BlsI GCNGC 7 cut(s) 118, 229, 530, 533, 536, 764, 1152
BmcAI AGTACT 1 cut(s) 521
Bme1390I CCNGG 7 cut(s) 479, 566, 842, 1003, 1218, 1356, 1566
Bme18I GGWCC 2 cut(s) 1118, 1204
BmeRI GACNNNNNGTC 1 cut(s) 718
BmeT110I CYCGRG 4 cut(s) 779, 1013, 1122, 1154
BmgBI CACGTC 3 cut(s) 615, 898, 903
BmgT120I GGNCC 6 cut(s) 475, 574, 657, 733, 1118, 1204
BmiI GGNNCC 8 cut(s) 193, 766, 1119, 1206, 1245, 1403, 1497, 1563
BmrFI CCNGG 7 cut(s) 479, 566, 842, 1003, 1218, 1356, 1566
BmrI ACTGGG 1 cut(s) 418
BmsI GCATC 1 cut(s) 999
BmtI GCTAGC 1 cut(s) 1433
BmuI ACTGGG 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 1609
BpmI CTGGAG 1 cut(s) 1528
BpuEI CTTGAG 2 cut(s) 1054, 1605
BpuMI CCSGG 3 cut(s) 842, 1003, 1218
Bsa29I ATCGAT 1 cut(s) 1519
BsaAI YACGTR 2 cut(s) 592, 1240
BsaHI GRCGYC 2 cut(s) 492, 1244
BsaI GGTCTC 2 cut(s) 329, 1437
BsaWI WCCGGW 1 cut(s) 866
Bsc4I CCNNNNNNNGG 9 cut(s) 381, 571, 666, 841, 922, 1127, 1372, 1453, 1492
Bse118I RCCGGY 2 cut(s) 814, 1555
Bse1I ACTGG 5 cut(s) 413, 689, 1434, 1458, 1511
Bse3DI GCAATG 2 cut(s) 52, 750
BseBI CCWGG 4 cut(s) 479, 566, 1356, 1566
BseCI ATCGAT 1 cut(s) 1519
BseGI GGATG 2 cut(s) 456, 1402
BseLI CCNNNNNNNGG 9 cut(s) 381, 571, 666, 841, 922, 1127, 1372, 1453, 1492
BseMI GCAATG 2 cut(s) 52, 750
BseMII CTCAG 2 cut(s) 68, 441
BseNI ACTGG 5 cut(s) 413, 689, 1434, 1458, 1511
BseRI GAGGAG 1 cut(s) 431
BseSI GKGCMC 3 cut(s) 842, 1008, 1095
BseX3I CGGCCG 2 cut(s) 1553, 1575
BseXI GCAGC 3 cut(s) 128, 518, 540
BseYI CCCAGC 1 cut(s) 1447
BsgI GTGCAG 1 cut(s) 118
Bsh1236I CGCG 7 cut(s) 544, 598, 727, 762, 824, 1022, 1040
Bsh1285I CGRYCG 3 cut(s) 1523, 1556, 1578
BshNI GGYRCC 3 cut(s) 764, 1243, 1401
BshVI ATCGAT 1 cut(s) 1519
BsiEI CGRYCG 3 cut(s) 1523, 1556, 1578
BsiHKAI GWGCWC 1 cut(s) 456
BsiHKCI CYCGRG 4 cut(s) 779, 1013, 1122, 1154
BsiSI CCGG 6 cut(s) 815, 842, 867, 1002, 1217, 1556
BsiWI CGTACG 1 cut(s) 1041
BslFI GGGAC 3 cut(s) 547, 634, 1190
BslI CCNNNNNNNGG 9 cut(s) 381, 571, 666, 841, 922, 1127, 1372, 1453, 1492
BsmAI GTCTC 4 cut(s) 111, 329, 735, 1437
BsmBI CGTCTC 1 cut(s) 111
BsmFI GGGAC 3 cut(s) 547, 634, 1190
Bso31I GGTCTC 2 cut(s) 329, 1437
BsoBI CYCGRG 4 cut(s) 779, 1013, 1122, 1154
Bsp1286I GDGCHC 4 cut(s) 456, 842, 1008, 1095
Bsp19I CCATGG 1 cut(s) 1405
BspCNI CTCAG 2 cut(s) 69, 442
BspDI ATCGAT 1 cut(s) 1519
BspFNI CGCG 7 cut(s) 544, 598, 727, 762, 824, 1022, 1040
BspHI TCATGA 1 cut(s) 1254
BspLI GGNNCC 8 cut(s) 193, 766, 1119, 1206, 1245, 1403, 1497, 1563
BspOI GCTAGC 1 cut(s) 1433
BspPI GGATC 7 cut(s) 500, 794, 852, 885, 1323, 1556, 1569
BspQI GCTCTTC 1 cut(s) 7
BspT107I GGYRCC 3 cut(s) 764, 1243, 1401
BspTI CTTAAG 1 cut(s) 1307
BspTNI GGTCTC 2 cut(s) 329, 1437
BsrBI CCGCTC 1 cut(s) 1150
BsrDI GCAATG 2 cut(s) 52, 750
BsrFI RCCGGY 2 cut(s) 814, 1555
BsrI ACTGG 5 cut(s) 413, 689, 1434, 1458, 1511
BssAI RCCGGY 2 cut(s) 814, 1555
BssNI GRCGYC 2 cut(s) 492, 1244
BssT1I CCWWGG 2 cut(s) 660, 1405
Bst2UI CCWGG 4 cut(s) 479, 566, 1356, 1566
Bst4CI ACNGT 8 cut(s) 139, 208, 223, 398, 524, 769, 853, 957
Bst6I CTCTTC 4 cut(s) 7, 306, 611, 1435
BstACI GRCGYC 2 cut(s) 492, 1244
BstAFI CTTAAG 1 cut(s) 1307
BstBAI YACGTR 2 cut(s) 592, 1240
BstC8I GCNNGC 4 cut(s) 27, 484, 656, 1431
BstDEI CTNAG 2 cut(s) 77, 450
BstDSI CCRYGG 3 cut(s) 394, 1277, 1405
BstF5I GGATG 2 cut(s) 456, 1402
BstFNI CGCG 7 cut(s) 544, 598, 727, 762, 824, 1022, 1040
BstH2I RGCGCY 1 cut(s) 1247
BstHHI GCGC 9 cut(s) 6, 546, 600, 727, 762, 1022, 1040, 1246, 1364
BstMAI GTCTC 4 cut(s) 111, 329, 735, 1437
BstMCI CGRYCG 3 cut(s) 1523, 1556, 1578
BstMWI GCNNNNNNNGC 7 cut(s) 534, 543, 912, 1017, 1090, 1531, 1552
BstNI CCWGG 4 cut(s) 479, 566, 1356, 1566
BstNSI RCATGY 1 cut(s) 1295
BstSCI CCNGG 7 cut(s) 477, 564, 840, 1001, 1216, 1354, 1564
BstSLI GKGCMC 3 cut(s) 842, 1008, 1095
BstUI CGCG 7 cut(s) 544, 598, 727, 762, 824, 1022, 1040
BstV1I GCAGC 3 cut(s) 128, 518, 540
BstV2I GAAGAC 1 cut(s) 1609
BstX2I RGATCY 2 cut(s) 877, 1561
BstXI CCANNNNNNTGG 1 cut(s) 1506
BstYI RGATCY 2 cut(s) 877, 1561
BstZI CGGCCG 2 cut(s) 1553, 1575
Bsu15I ATCGAT 1 cut(s) 1519
BsuI GTATCC 1 cut(s) 1108
BsuTUI ATCGAT 1 cut(s) 1519
BtgI CCRYGG 3 cut(s) 394, 1277, 1405
BtrI CACGTC 3 cut(s) 615, 898, 903
BtsCI GGATG 2 cut(s) 456, 1402
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 4 cut(s) 13, 74, 1441, 1451
Cac8I GCNNGC 4 cut(s) 27, 484, 656, 1431
CciI TCATGA 1 cut(s) 1254
CfoI GCGC 9 cut(s) 6, 546, 600, 727, 762, 1022, 1040, 1246, 1364
Cfr10I RCCGGY 2 cut(s) 814, 1555
Cfr13I GGNCC 6 cut(s) 475, 574, 657, 733, 1118, 1204
ClaI ATCGAT 1 cut(s) 1519
CseI GACGC 3 cut(s) 53, 353, 500
Csp6I GTAC 3 cut(s) 520, 1042, 1402
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviAII CATG 5 cut(s) 571, 1255, 1292, 1406, 1535
CviQI GTAC 3 cut(s) 520, 1042, 1402
DdeI CTNAG 2 cut(s) 77, 450
DinI GGCGCC 1 cut(s) 1245
DraIII CACNNNGTG 1 cut(s) 1201
DrdI GACNNNNNNGTC 1 cut(s) 1085
DriI GACNNNNNGTC 1 cut(s) 718
DseDI GACNNNNNNGTC 1 cut(s) 1085
EaeI YGGCCR 2 cut(s) 1553, 1575
EagI CGGCCG 2 cut(s) 1553, 1575
Eam1104I CTCTTC 4 cut(s) 7, 306, 611, 1435
Eam1105I GACNNNNNGTC 1 cut(s) 718
EarI CTCTTC 4 cut(s) 7, 306, 611, 1435
Ecl136II GAGCTC 1 cut(s) 454
EclXI CGGCCG 2 cut(s) 1553, 1575
Eco130I CCWWGG 2 cut(s) 660, 1405
Eco147I AGGCCT 2 cut(s) 482, 1633
Eco24I GRGCYC 1 cut(s) 456
Eco31I GGTCTC 2 cut(s) 329, 1437
Eco47I GGWCC 2 cut(s) 1118, 1204
Eco52I CGGCCG 2 cut(s) 1553, 1575
Eco53kI GAGCTC 1 cut(s) 454
Eco72I CACGTG 2 cut(s) 592, 1240
Eco88I CYCGRG 4 cut(s) 779, 1013, 1122, 1154
EcoICRI GAGCTC 1 cut(s) 454
EcoO109I RGGNCCY 1 cut(s) 1118
EcoRII CCWGG 4 cut(s) 477, 564, 1354, 1564
EcoT14I CCWWGG 2 cut(s) 660, 1405
EcoT38I GRGCYC 1 cut(s) 456
EgeI GGCGCC 1 cut(s) 1245
EheI GGCGCC 1 cut(s) 1245
ErhI CCWWGG 2 cut(s) 660, 1405
Esp3I CGTCTC 1 cut(s) 111
FaeI CATG 5 cut(s) 574, 1258, 1295, 1409, 1538
FaqI GGGAC 3 cut(s) 547, 634, 1190
FatI CATG 5 cut(s) 570, 1254, 1291, 1405, 1534
FauI CCCGC 4 cut(s) 378, 659, 782, 1077
FblI GTMKAC 1 cut(s) 792
Fnu4HI GCNGC 7 cut(s) 117, 228, 529, 532, 535, 763, 1151
FokI GGATG 2 cut(s) 443, 1409
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 7 cut(s) 117, 228, 529, 532, 535, 763, 1151
FspBI CTAG 2 cut(s) 1430, 1629
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 9 cut(s) 5, 545, 599, 726, 761, 1021, 1039, 1245, 1363
GluI GCNGC 7 cut(s) 117, 228, 529, 532, 535, 763, 1151
GsaI CCCAGC 1 cut(s) 1451
GsuI CTGGAG 1 cut(s) 1528
HaeII RGCGCY 1 cut(s) 1247
HapII CCGG 6 cut(s) 815, 842, 867, 1002, 1217, 1556
HgaI GACGC 3 cut(s) 53, 353, 500
HhaI GCGC 9 cut(s) 6, 546, 600, 727, 762, 1022, 1040, 1246, 1364
Hin1I GRCGYC 2 cut(s) 492, 1244
Hin1II CATG 5 cut(s) 574, 1258, 1295, 1409, 1538
Hin6I GCGC 9 cut(s) 4, 544, 598, 725, 760, 1020, 1038, 1244, 1362
HinP1I GCGC 9 cut(s) 4, 544, 598, 725, 760, 1020, 1038, 1244, 1362
HincII GTYRAC 1 cut(s) 793
HindII GTYRAC 1 cut(s) 793
HinfI GANTC 7 cut(s) 257, 297, 560, 826, 831, 863, 1157
HpaII CCGG 6 cut(s) 815, 842, 867, 1002, 1217, 1556
HphI GGTGA 9 cut(s) 63, 78, 196, 268, 763, 1198, 1468, 1550, 1587
Hpy166II GTNNAC 5 cut(s) 301, 793, 1189, 1195, 1459
Hpy8I GTNNAC 5 cut(s) 301, 793, 1189, 1195, 1459
HpyAV CCTTC 5 cut(s) 157, 505, 520, 562, 638
HpyCH4III ACNGT 8 cut(s) 139, 208, 223, 398, 524, 769, 853, 957
HpyCH4IV ACGT 9 cut(s) 591, 614, 719, 897, 902, 1068, 1134, 1191, 1239
HpyCH4V TGCA 3 cut(s) 135, 1387, 1546
HpyF10VI GCNNNNNNNGC 7 cut(s) 534, 543, 912, 1017, 1090, 1531, 1552
HpyF3I CTNAG 2 cut(s) 77, 450
HpySE526I ACGT 9 cut(s) 591, 614, 719, 897, 902, 1068, 1134, 1191, 1239
Hsp92I GRCGYC 2 cut(s) 492, 1244
Hsp92II CATG 5 cut(s) 574, 1258, 1295, 1409, 1538
HspAI GCGC 9 cut(s) 4, 544, 598, 725, 760, 1020, 1038, 1244, 1362
KasI GGCGCC 1 cut(s) 1243
KpnI GGTACC 1 cut(s) 1405
LguI GCTCTTC 1 cut(s) 7
LmnI GCTCC 6 cut(s) 34, 669, 931, 1017, 1147, 1281
Lsp1109I GCAGC 3 cut(s) 128, 518, 540
LweI GCATC 1 cut(s) 999
MaeI CTAG 2 cut(s) 1430, 1629
MaeII ACGT 9 cut(s) 591, 614, 719, 897, 902, 1068, 1134, 1191, 1239
MaeIII GTNAC 3 cut(s) 417, 769, 898
MbiI CCGCTC 1 cut(s) 1150
MboII GAAGA 8 cut(s) 24, 134, 323, 547, 598, 631, 1452, 1614
MflI RGATCY 2 cut(s) 877, 1561
MhlI GDGCHC 4 cut(s) 456, 842, 1008, 1095
MluCI AATT 3 cut(s) 983, 1259, 1369
Mly113I GGCGCC 1 cut(s) 1244
MlyI GAGTC 3 cut(s) 306, 569, 1166
MmeI TCCRAC 2 cut(s) 1056, 1276
MseI TTAA 6 cut(s) 797, 951, 1143, 1170, 1230, 1308
MspA1I CMGCKG 2 cut(s) 531, 654
MspCI CTTAAG 1 cut(s) 1307
MspI CCGG 6 cut(s) 815, 842, 867, 1002, 1217, 1556
MspR9I CCNGG 7 cut(s) 479, 566, 842, 1003, 1218, 1356, 1566
MvaI CCWGG 4 cut(s) 479, 566, 1356, 1566
MvnI CGCG 7 cut(s) 544, 598, 727, 762, 824, 1022, 1040
MwoI GCNNNNNNNGC 7 cut(s) 534, 543, 912, 1017, 1090, 1531, 1552
NarI GGCGCC 1 cut(s) 1244
NciI CCSGG 3 cut(s) 842, 1003, 1218
NcoI CCATGG 1 cut(s) 1405
NheI GCTAGC 1 cut(s) 1429
NlaIII CATG 5 cut(s) 574, 1258, 1295, 1409, 1538
NlaIV GGNNCC 8 cut(s) 193, 766, 1119, 1206, 1245, 1403, 1497, 1563
NmeAIII GCCGAG 2 cut(s) 902, 1272
NmuCI GTSAC 2 cut(s) 769, 898
NsbI TGCGCA 1 cut(s) 5
NspI RCATGY 1 cut(s) 1295
PaeR7I CTCGAG 2 cut(s) 779, 1154
PagI TCATGA 1 cut(s) 1254
PasI CCCWGGG 1 cut(s) 565
PceI AGGCCT 2 cut(s) 482, 1633
PciSI GCTCTTC 1 cut(s) 7
PcsI WCGNNNNNNNCGW 7 cut(s) 420, 495, 555, 588, 786, 1050, 1086
PfeI GAWTC 4 cut(s) 257, 826, 831, 863
Pfl23II CGTACG 1 cut(s) 1041
PflFI GACNNNGTC 1 cut(s) 410
PflMI CCANNNNNTGG 3 cut(s) 666, 1372, 1492
PkrI GCNGC 7 cut(s) 118, 229, 530, 533, 536, 764, 1152
Ple19I CGATCG 1 cut(s) 1523
PleI GAGTC 3 cut(s) 305, 568, 1165
PluTI GGCGCC 1 cut(s) 1247
PmaCI CACGTG 2 cut(s) 592, 1240
PmlI CACGTG 2 cut(s) 592, 1240
PpsI GAGTC 3 cut(s) 305, 568, 1165
Ppu21I YACGTR 2 cut(s) 592, 1240
PpuMI RGGWCCY 1 cut(s) 1118
Psp124BI GAGCTC 1 cut(s) 456
Psp1406I AACGTT 1 cut(s) 1191
Psp5II RGGWCCY 1 cut(s) 1118
Psp6I CCWGG 4 cut(s) 477, 564, 1354, 1564
PspCI CACGTG 2 cut(s) 592, 1240
PspFI CCCAGC 1 cut(s) 1447
PspGI CCWGG 4 cut(s) 477, 564, 1354, 1564
PspLI CGTACG 1 cut(s) 1041
PspN4I GGNNCC 8 cut(s) 193, 766, 1119, 1206, 1245, 1403, 1497, 1563
PspPI GGNCC 6 cut(s) 475, 574, 657, 733, 1118, 1204
PspPPI RGGWCCY 1 cut(s) 1118
PspXI VCTCGAGB 2 cut(s) 779, 1154
PsuI RGATCY 2 cut(s) 877, 1561
PsyI GACNNNGTC 1 cut(s) 410
PvuI CGATCG 1 cut(s) 1523
PvuII CAGCTG 1 cut(s) 531
RsaI GTAC 3 cut(s) 521, 1043, 1403
RsaNI GTAC 3 cut(s) 520, 1042, 1402
SacI GAGCTC 1 cut(s) 456
SalI GTCGAC 1 cut(s) 791
SapI GCTCTTC 1 cut(s) 7
SaqAI TTAA 6 cut(s) 797, 951, 1143, 1170, 1230, 1308
SatI GCNGC 7 cut(s) 117, 228, 529, 532, 535, 763, 1151
Sau96I GGNCC 6 cut(s) 475, 574, 657, 733, 1118, 1204
ScaI AGTACT 1 cut(s) 521
SchI GAGTC 3 cut(s) 306, 569, 1166
ScrFI CCNGG 7 cut(s) 479, 566, 842, 1003, 1218, 1356, 1566
SduI GDGCHC 4 cut(s) 456, 842, 1008, 1095
SfaNI GCATC 1 cut(s) 999
SfoI GGCGCC 1 cut(s) 1245
Sfr274I CTCGAG 2 cut(s) 779, 1154
SinI GGWCC 2 cut(s) 1118, 1204
SlaI CTCGAG 2 cut(s) 779, 1154
SmlI CTYRAG 5 cut(s) 779, 1033, 1154, 1307, 1620
SmoI CTYRAG 5 cut(s) 779, 1033, 1154, 1307, 1620
Sse9I AATT 3 cut(s) 983, 1259, 1369
SseBI AGGCCT 2 cut(s) 482, 1633
SspDI GGCGCC 1 cut(s) 1243
SspMI CTAG 2 cut(s) 1430, 1629
SstI GAGCTC 1 cut(s) 456
StuI AGGCCT 2 cut(s) 482, 1633
StyD4I CCNGG 7 cut(s) 477, 564, 840, 1001, 1216, 1354, 1564
StyI CCWWGG 2 cut(s) 660, 1405
TaaI ACNGT 8 cut(s) 139, 208, 223, 398, 524, 769, 853, 957
TaiI ACGT 9 cut(s) 594, 617, 722, 900, 905, 1071, 1137, 1194, 1242
TaqII GACCGA 1 cut(s) 353
TasI AATT 3 cut(s) 983, 1259, 1369
TatI WGTACW 1 cut(s) 519
TauI GCSGC 4 cut(s) 230, 537, 765, 1153
TfiI GAWTC 4 cut(s) 257, 826, 831, 863
Tru1I TTAA 6 cut(s) 797, 951, 1143, 1170, 1230, 1308
Tru9I TTAA 6 cut(s) 797, 951, 1143, 1170, 1230, 1308
TscAI CASTG 4 cut(s) 13, 81, 1441, 1458
TseFI GTSAC 2 cut(s) 769, 898
TseI GCWGC 3 cut(s) 116, 528, 531
Tsp45I GTSAC 2 cut(s) 769, 898
TspDTI ATGAA 3 cut(s) 87, 342, 1271
TspGWI ACGGA 2 cut(s) 959, 1090
TspRI CASTG 4 cut(s) 13, 81, 1441, 1458
Tth111I GACNNNGTC 1 cut(s) 410
Van91I CCANNNNNTGG 3 cut(s) 666, 1372, 1492
Vha464I CTTAAG 1 cut(s) 1307
VpaK11BI GGWCC 2 cut(s) 1118, 1204
XceI RCATGY 1 cut(s) 1295
XhoI CTCGAG 2 cut(s) 779, 1154
XmiI GTMKAC 1 cut(s) 792
XspI CTAG 2 cut(s) 1430, 1629
ZrmI AGTACT 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.