pycom10g18580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0018713
Physical Location & Seq
Forward (+)
21815867 .. 21816103
237 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 237 bp
ATGTCAGGAGCACAGGGAGCTCAGCCGCCGGGGTCACAGACGGCGACAACGTACGAGTCGGTTCAAGGAGGGGAGAACAAGAGCAGGACGGAGCTACGATCCCAGCAGGACCAGGGTGGCGTTCAGGTTGACAAGCTCCAAGACAAGGTGGAGGATGCTGCCGGCAAAGGTGGCCCCGTCTTTGGCGCCGGAAAAGACCCGAATAAAAAGGACCTCGGTGTCTCCGGCACTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

79

Amino Acids

7.89

Weight (kDa)

5.2

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018713)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G21820
malus_domestica MD05G1241500.v1.1
pyrus_communis pycom10g18580
rosa_chinensis RchiOBHm_Chr5g0022881
rosa_multiflora Rmu_sc0010181.1_g000003
rosa_samantha Rh5BG160800 Rh5CG175400 Rh5DG162100
rosa_wichuraiana Rw5G014550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccB1I GGYRCC 1 cut(s) 185
AciI CCGC 1 cut(s) 26
AclWI GGATC 1 cut(s) 93
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 1 cut(s) 53
AfiI CCNNNNNNNGG 2 cut(s) 145, 182
AgsI TTSAA 1 cut(s) 65
AjnI CCWGG 1 cut(s) 111
AluBI AGCT 3 cut(s) 20, 94, 136
AluI AGCT 3 cut(s) 20, 94, 136
Alw21I GWGCWC 2 cut(s) 13, 22
Alw26I GTCTC 1 cut(s) 226
AlwI GGATC 1 cut(s) 93
AoxI GGCC 1 cut(s) 172
ApeKI GCWGC 1 cut(s) 158
AspLEI GCGC 1 cut(s) 188
AspS9I GGNCC 3 cut(s) 109, 173, 211
AsuC2I CCSGG 1 cut(s) 30
AvaII GGWCC 2 cut(s) 109, 211
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 22
Bbv12I GWGCWC 2 cut(s) 13, 22
BbvI GCAGC 1 cut(s) 145
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 1 cut(s) 113
BcnI CCSGG 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 226
BfoI RGCGCY 1 cut(s) 189
BisI GCNGC 2 cut(s) 26, 159
BlpI GCTNAGC 1 cut(s) 21
BlsI GCNGC 2 cut(s) 27, 160
Bme1390I CCNGG 2 cut(s) 30, 113
Bme18I GGWCC 2 cut(s) 109, 211
BmgT120I GGNCC 3 cut(s) 109, 173, 211
BmiI GGNNCC 2 cut(s) 175, 187
BmrFI CCNGG 2 cut(s) 30, 113
BmsI GCATC 1 cut(s) 145
Bpu1102I GCTNAGC 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 186
BsaJI CCNNGG 3 cut(s) 29, 112, 214
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 182
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 1 cut(s) 235
BseBI CCWGG 1 cut(s) 113
BseDI CCNNGG 3 cut(s) 29, 112, 214
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 2 cut(s) 145, 182
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 1 cut(s) 235
BseXI GCAGC 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 102
BshFI GGCC 1 cut(s) 174
BshNI GGYRCC 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 13, 22
BsiSI CCGG 4 cut(s) 29, 162, 189, 225
BsiWI CGTACG 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 145, 182
BsmAI GTCTC 1 cut(s) 226
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 2 cut(s) 13, 22
Bsp143I GATC 1 cut(s) 98
Bsp1720I GCTNAGC 1 cut(s) 21
BspACI CCGC 1 cut(s) 26
BspANI GGCC 1 cut(s) 174
BspCNI CTCAG 1 cut(s) 34
BspLI GGNNCC 2 cut(s) 175, 187
BspPI GGATC 1 cut(s) 93
BspT107I GGYRCC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 1 cut(s) 235
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 3 cut(s) 29, 112, 214
BssMI GATC 1 cut(s) 98
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 113
BstACI GRCGYC 1 cut(s) 186
BstC8I GCNNGC 1 cut(s) 163
BstDEI CTNAG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 160
BstH2I RGCGCY 1 cut(s) 189
BstHHI GCGC 1 cut(s) 188
BstKTI GATC 1 cut(s) 101
BstMAI GTCTC 1 cut(s) 226
BstMBI GATC 1 cut(s) 98
BstMWI GCNNNNNNNGC 2 cut(s) 17, 171
BstNI CCWGG 1 cut(s) 113
BstSCI CCNGG 2 cut(s) 28, 111
BstV1I GCAGC 1 cut(s) 145
BsuRI GGCC 1 cut(s) 174
BtsCI GGATG 1 cut(s) 160
BtsIMutI CAGTG 1 cut(s) 228
Cac8I GCNNGC 1 cut(s) 163
CfoI GCGC 1 cut(s) 188
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 3 cut(s) 109, 173, 211
Csp6I GTAC 1 cut(s) 52
CviJI RGCY 6 cut(s) 20, 25, 94, 136, 174, 234
CviKI_1 RGCY 6 cut(s) 20, 25, 94, 136, 174, 234
CviQI GTAC 1 cut(s) 52
DdeI CTNAG 1 cut(s) 21
DinI GGCGCC 1 cut(s) 187
DpnI GATC 1 cut(s) 100
DpnII GATC 1 cut(s) 98
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
Ecl136II GAGCTC 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 22
Eco47I GGWCC 2 cut(s) 109, 211
Eco53kI GAGCTC 1 cut(s) 20
EcoICRI GAGCTC 1 cut(s) 20
EcoO109I RGGNCCY 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 111
EcoT38I GRGCYC 1 cut(s) 22
EgeI GGCGCC 1 cut(s) 187
EheI GGCGCC 1 cut(s) 187
Fnu4HI GCNGC 2 cut(s) 26, 159
FokI GGATG 1 cut(s) 167
FriOI GRGCYC 1 cut(s) 22
Fsp4HI GCNGC 2 cut(s) 26, 159
GlaI GCGC 1 cut(s) 187
GluI GCNGC 2 cut(s) 26, 159
GsaI CCCAGC 1 cut(s) 106
HaeII RGCGCY 1 cut(s) 189
HaeIII GGCC 1 cut(s) 174
HapII CCGG 4 cut(s) 29, 162, 189, 225
HhaI GCGC 1 cut(s) 188
Hin1I GRCGYC 1 cut(s) 186
Hin6I GCGC 1 cut(s) 186
HinP1I GCGC 1 cut(s) 186
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HinfI GANTC 1 cut(s) 56
HpaII CCGG 4 cut(s) 29, 162, 189, 225
Hpy166II GTNNAC 1 cut(s) 130
Hpy188III TCNNGA 1 cut(s) 6
Hpy8I GTNNAC 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 171
HpyF3I CTNAG 1 cut(s) 21
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 186
HspAI GCGC 1 cut(s) 186
KasI GGCGCC 1 cut(s) 185
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Kzo9I GATC 1 cut(s) 98
LmnI GCTCC 4 cut(s) 8, 17, 91, 141
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 1 cut(s) 145
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 33
MalI GATC 1 cut(s) 100
MboI GATC 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 13, 22
Mly113I GGCGCC 1 cut(s) 186
MlyI GAGTC 1 cut(s) 65
MnlI CCTC 3 cut(s) 62, 145, 224
MroNI GCCGGC 1 cut(s) 161
MspI CCGG 4 cut(s) 29, 162, 189, 225
MspR9I CCNGG 2 cut(s) 30, 113
MvaI CCWGG 1 cut(s) 113
MwoI GCNNNNNNNGC 2 cut(s) 17, 171
NaeI GCCGGC 1 cut(s) 163
NarI GGCGCC 1 cut(s) 186
NciI CCSGG 1 cut(s) 30
NdeII GATC 1 cut(s) 98
NgoMIV GCCGGC 1 cut(s) 161
NlaIV GGNNCC 2 cut(s) 175, 187
NmuCI GTSAC 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PdiI GCCGGC 1 cut(s) 163
Pfl23II CGTACG 1 cut(s) 51
PkrI GCNGC 2 cut(s) 27, 160
PleI GAGTC 1 cut(s) 64
PluTI GGCGCC 1 cut(s) 189
PpsI GAGTC 1 cut(s) 64
PpuMI RGGWCCY 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 22
Psp5II RGGWCCY 1 cut(s) 211
Psp6I CCWGG 1 cut(s) 111
PspFI CCCAGC 1 cut(s) 102
PspGI CCWGG 1 cut(s) 111
PspLI CGTACG 1 cut(s) 51
PspN4I GGNNCC 2 cut(s) 175, 187
PspPI GGNCC 3 cut(s) 109, 173, 211
PspPPI RGGWCCY 1 cut(s) 211
RsaI GTAC 1 cut(s) 53
RsaNI GTAC 1 cut(s) 52
SacI GAGCTC 1 cut(s) 22
SatI GCNGC 2 cut(s) 26, 159
Sau3AI GATC 1 cut(s) 98
Sau96I GGNCC 3 cut(s) 109, 173, 211
SchI GAGTC 1 cut(s) 65
ScrFI CCNGG 2 cut(s) 30, 113
SduI GDGCHC 2 cut(s) 13, 22
SetI ASST 8 cut(s) 22, 53, 96, 129, 138, 150, 172, 216
SfaNI GCATC 1 cut(s) 145
SfoI GGCGCC 1 cut(s) 187
SinI GGWCC 2 cut(s) 109, 211
SsiI CCGC 1 cut(s) 26
SspDI GGCGCC 1 cut(s) 185
SstI GAGCTC 1 cut(s) 22
StyD4I CCNGG 2 cut(s) 28, 111
TaiI ACGT 1 cut(s) 53
TauI GCSGC 1 cut(s) 28
TscAI CASTG 1 cut(s) 235
TseFI GTSAC 1 cut(s) 33
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 33
TspGWI ACGGA 1 cut(s) 104
TspRI CASTG 1 cut(s) 235
VpaK11BI GGWCC 2 cut(s) 109, 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.