pycom10g19620

acyltransferase-like 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0016182
Physical Location & Seq
Forward (+)
22665872 .. 22670067
4196 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1605 bp
ATGGCGATGCTGTTGGAGGATATTTTGAAATCGGTGGAGCTCTGGTTGAAGCTGATCAAGAAGCCCCAGCCCTACGTGGACCCTCATCTCGACCCGGTTCTGCTGGTTCCGGGTATCGCCGGTTCGGTCTTGAACGCGGTCGATGAAGAGAGTGGTGCGGAGGAGCGCGTCTGGATTCGGATCATTGGTGCTGACTACACGTTTCGGACCAAGCTTTGGTCTCGTATCGATCCTTCAACAGGTAAAACTGAGTCTATGGATCCAAAAGCAAGGATAGTGGTCCCTGAAGGTAGATATGGACTTCAGGCAATTGACGCCTTGGACCCTGACATGGTCTTTGGACAGGAATGTGTATATTATTTCCATGACATGATTGTTGAATTGACCAAGTGGGGTTTCCAAGAGGGGAAAACACTTTTTGGGTTTGGATATGATTTCCGCCAAAGCAACAGGCTGCAGGAGACATTGGATCGATTAGCTGCAAAATTAGAGGCAGTGTATGAGGCTTCTGGAGGGAAAAAGATAACCATCATAACTCATTCTATGGGGGGCCTTCTTGTGAAATGTTTCATGTGCTTGCATAGTGATGTTTTTGAGAAATATGTGAACAAGTGGATTGCAGTTGCTGCACCATTCCAGGGTGCACCTGGATATGTTACATCTACCTTTTTAAATGGAATGTCATTTGTTGATGGGTGGGAACAGAATTTTTTTATATCAAAATGGAGCATGCACCAACTGCTTATTGAGTGCCCATCAATATATGAGTTGATGGCTTGTCTTGATTTTCAATGGGAACATCCTCCGCTTCTGGAAGTGTGGAGAGGTAGGCCTGATGGTGATGGGAAATCTCAAATTATGCTAGAGTCTTACCCCCTGGTAGAGAGCGTACAGATTTTTAAGGAAGCTCTTGCAAATAATACGGTCAATTATAACGGTCAAGATATTCCCCTACCATTTAATATGGAAATCTTGAAATGGGCTAATGAAACACGCAAGATTATATCTCAAGCGAAACTTCCTCCTCAAGTTAAATTCTACAATATATATGGGATGAATCTCGACACACCTCATAGTGTTTGCTATGGAAATGAACAAACACCTGTCACAGATCTACAACAGCTACGATATTTCCAGCCTAATTATGTATGCGTTGATGGTGATGGGACAGTCCCAGTGGAATCGGCTAAGGCAGATGGGCTTAATGCTGTCGCAAGGGTTGGAGTCCCTGGTGAGCACCGAGGAATTCTCTGTGAGCACCATGTATTCCGGATACTGAAGCACTGGTTGAAGGCAGACCATGACCCGTACTACAACCCTGTAAACGATTATGTGATTCTACCCACTGCGTTTGAAATGGAGAAGCACAAAGAGAAAGGTCTAGAAGTAACTTCTCTAAAAGAGGAGTGGGAAATCATCTCAGAATGCCAAGACGCTGATCACAAGAATTCTCGTTCAGCCGCCGCTGATGAAAAGCCTGTGGTGAGTTCCATATCCGTTTCTGAAGTGGGAGCTGAGGCCTGCGCAACTCTTACTGTTCACCCTCATAGCGAGGGAAAGCAGCATGTGGAGCTGAATGCTCTAAGTGTGTCAGTCGATGCCTGA

Protein Analysis

535

Amino Acids

60.19

Weight (kDa)

5.15

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LCAT PF02450 87 - 397 5.2e-27 Lecithin:cholesterol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G19860
fragaria_vesca FvH4_3g12200
malus_domestica MD05G1252800.v1.1 MD10G1233900.v1.1
prunus_persica Prupe.4G111100_v2.0.a1
pyrus_communis pycom05g23100 pycom10g19620
rosa_chinensis RchiOBHm_Chr5g0020001
rosa_laevigata RLG00000032492
rosa_multiflora Rmu_sc0002250.1_g000005
rosa_roxburghii Rroxscaffold_1G00058190
rosa_rugosa Rorug05G0056100
rosa_samantha Rh5AG146300 Rh5BG145800
rosa_wichuraiana Rw5G012920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 933
Acc16I TGCGCA 1 cut(s) 1525
AccB7I CCANNNNNTGG 1 cut(s) 216
AccII CGCG 2 cut(s) 137, 168
AccIII TCCGGA 1 cut(s) 1269
AciI CCGC 6 cut(s) 137, 158, 439, 806, 1461, 1464
AclWI GGATC 5 cut(s) 188, 224, 254, 267, 477
AcsI RAATTY 4 cut(s) 706, 1034, 1245, 1447
AcuI CTGAAG 4 cut(s) 287, 306, 1298, 1524
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 2 cut(s) 891, 1310
AfiI CCNNNNNNNGG 4 cut(s) 216, 239, 331, 638
AflIII ACRYGT 1 cut(s) 198
AgsI TTSAA 9 cut(s) 28, 49, 133, 237, 380, 791, 976, 1291, 1355
AjnI CCWGG 4 cut(s) 636, 646, 876, 1228
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AluBI AGCT 8 cut(s) 40, 52, 214, 479, 908, 1123, 1514, 1573
AluI AGCT 8 cut(s) 40, 52, 214, 479, 908, 1123, 1514, 1573
Alw21I GWGCWC 4 cut(s) 42, 646, 1239, 1260
Alw26I GTCTC 2 cut(s) 225, 455
Alw44I GTGCAC 1 cut(s) 642
AlwI GGATC 5 cut(s) 188, 224, 254, 267, 477
AlwNI CAGNNNCTG 1 cut(s) 626
Aor13HI TCCGGA 1 cut(s) 1269
AoxI GGCC 3 cut(s) 550, 830, 1518
ApaLI GTGCAC 1 cut(s) 642
ApeKI GCWGC 4 cut(s) 454, 479, 626, 1561
ApoI RAATTY 4 cut(s) 706, 1034, 1245, 1447
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 1 cut(s) 566
AspLEI GCGC 2 cut(s) 168, 1526
AspS9I GGNCC 5 cut(s) 79, 207, 280, 322, 550
AsuC2I CCSGG 2 cut(s) 95, 111
AsuHPI GGTGA 5 cut(s) 851, 1172, 1244, 1495, 1532
AvaII GGWCC 4 cut(s) 79, 207, 280, 322
BaeGI GKGCMC 2 cut(s) 646, 755
BamHI GGATCC 1 cut(s) 259
BanII GRGCYC 1 cut(s) 42
BarI GAAGNNNNNNTAC 2 cut(s) 217, 249
Bbv12I GWGCWC 4 cut(s) 42, 646, 1239, 1260
BbvCI CCTCAGC 1 cut(s) 1515
BbvI GCAGC 4 cut(s) 441, 466, 613, 1573
BccI CCATC 9 cut(s) 536, 686, 763, 766, 830, 836, 1151, 1157, 1190
BciT130I CCWGG 4 cut(s) 638, 648, 878, 1230
BciVI GTATCC 1 cut(s) 1266
BclI TGATCA 2 cut(s) 54, 1438
BcnI CCSGG 2 cut(s) 95, 111
BcoDI GTCTC 2 cut(s) 225, 455
BfaI CTAG 2 cut(s) 863, 1382
BfmI CTRYAG 1 cut(s) 455
BfuI GTATCC 1 cut(s) 1266
BglII AGATCT 1 cut(s) 1111
BisI GCNGC 6 cut(s) 455, 480, 627, 1461, 1464, 1562
BlsI GCNGC 6 cut(s) 456, 481, 628, 1462, 1465, 1563
Bme1390I CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
Bme18I GGWCC 4 cut(s) 79, 207, 280, 322
BmgT120I GGNCC 5 cut(s) 79, 207, 280, 322, 550
BmiI GGNNCC 6 cut(s) 81, 108, 261, 282, 324, 551
BmrFI CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
BmrI ACTGGG 1 cut(s) 1169
BmsI GCATC 1 cut(s) 1588
BmuI ACTGGG 1 cut(s) 1169
BplI GAGNNNNNCTC 2 cut(s) 1233, 1265
BpmI CTGGAG 1 cut(s) 531
Bpu10I CCTNAGC 2 cut(s) 1188, 1515
BpuEI CTTGAG 2 cut(s) 993, 1011
BpuMI CCSGG 2 cut(s) 95, 111
Bsa29I ATCGAT 2 cut(s) 228, 472
BsaAI YACGTR 1 cut(s) 76
BsaBI GATNNNNATC 2 cut(s) 179, 527
BsaHI GRCGYC 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 225
BsaJI CCNNGG 5 cut(s) 318, 637, 876, 1228, 1240
BsaWI WCCGGW 1 cut(s) 1269
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 4 cut(s) 216, 239, 331, 638
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 2 cut(s) 1175, 1289
Bse8I GATNNNNATC 2 cut(s) 179, 527
BseAI TCCGGA 1 cut(s) 1269
BseBI CCWGG 4 cut(s) 638, 648, 878, 1230
BseCI ATCGAT 2 cut(s) 228, 472
BseDI CCNNGG 5 cut(s) 318, 637, 876, 1228, 1240
BseGI GGATG 2 cut(s) 799, 1059
BseJI GATNNNNATC 2 cut(s) 179, 527
BseLI CCNNNNNNNGG 4 cut(s) 216, 239, 331, 638
BseMII CTCAG 3 cut(s) 240, 1434, 1506
BseNI ACTGG 2 cut(s) 1175, 1289
BseRI GAGGAG 3 cut(s) 176, 1014, 1418
BseSI GKGCMC 2 cut(s) 646, 755
BseXI GCAGC 4 cut(s) 441, 466, 613, 1573
BseYI CCCAGC 1 cut(s) 66
BsgI GTGCAG 1 cut(s) 612
Bsh1236I CGCG 2 cut(s) 137, 168
Bsh1285I CGRYCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 552, 832, 1520
BshVI ATCGAT 2 cut(s) 228, 472
BsiEI CGRYCG 1 cut(s) 141
BsiHKAI GWGCWC 4 cut(s) 42, 646, 1239, 1260
BsiSI CCGG 4 cut(s) 95, 110, 120, 1270
BslFI GGGAC 4 cut(s) 266, 1157, 1180, 1211
BslI CCNNNNNNNGG 4 cut(s) 216, 239, 331, 638
BsmAI GTCTC 2 cut(s) 225, 455
BsmFI GGGAC 4 cut(s) 266, 1157, 1180, 1211
BsmI GAATGC 2 cut(s) 1430, 1582
BsnI GGCC 3 cut(s) 552, 832, 1520
Bso31I GGTCTC 1 cut(s) 225
Bsp1286I GDGCHC 5 cut(s) 42, 646, 755, 1239, 1260
Bsp13I TCCGGA 1 cut(s) 1269
Bsp143I GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
BspACI CCGC 6 cut(s) 137, 158, 439, 806, 1461, 1464
BspANI GGCC 3 cut(s) 552, 832, 1520
BspCNI CTCAG 3 cut(s) 241, 1433, 1507
BspDI ATCGAT 2 cut(s) 228, 472
BspEI TCCGGA 1 cut(s) 1269
BspFNI CGCG 2 cut(s) 137, 168
BspLI GGNNCC 6 cut(s) 81, 108, 261, 282, 324, 551
BspMAI CTGCAG 1 cut(s) 459
BspPI GGATC 5 cut(s) 188, 224, 254, 267, 477
BspTNI GGTCTC 1 cut(s) 225
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 2 cut(s) 1175, 1289
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 5 cut(s) 318, 637, 876, 1228, 1240
BssMI GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 4 cut(s) 638, 648, 878, 1230
Bst4CI ACNGT 4 cut(s) 925, 938, 1171, 1537
Bst6I CTCTTC 1 cut(s) 141
BstACI GRCGYC 1 cut(s) 315
BstAPI GCANNNNNTGC 2 cut(s) 626, 739
BstBAI YACGTR 1 cut(s) 76
BstC8I GCNNGC 3 cut(s) 578, 731, 1522
BstDEI CTNAG 5 cut(s) 249, 1188, 1420, 1515, 1583
BstENI CCTNNNNNAGG 1 cut(s) 237
BstF5I GGATG 2 cut(s) 799, 1059
BstFNI CGCG 2 cut(s) 137, 168
BstHHI GCGC 2 cut(s) 168, 1526
BstKTI GATC 7 cut(s) 57, 183, 232, 262, 472, 1114, 1441
BstMAI GTCTC 2 cut(s) 225, 455
BstMBI GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
BstMCI CGRYCG 1 cut(s) 141
BstMWI GCNNNNNNNGC 4 cut(s) 314, 626, 739, 1570
BstNI CCWGG 4 cut(s) 638, 648, 878, 1230
BstNSI RCATGY 2 cut(s) 733, 1568
BstSCI CCNGG 6 cut(s) 93, 109, 636, 646, 876, 1228
BstSFI CTRYAG 1 cut(s) 455
BstSLI GKGCMC 2 cut(s) 646, 755
BstUI CGCG 2 cut(s) 137, 168
BstV1I GCAGC 4 cut(s) 441, 466, 613, 1573
BstX2I RGATCY 2 cut(s) 259, 1111
BstYI RGATCY 2 cut(s) 259, 1111
Bsu15I ATCGAT 2 cut(s) 228, 472
BsuI GTATCC 1 cut(s) 1266
BsuRI GGCC 3 cut(s) 552, 832, 1520
BsuTUI ATCGAT 2 cut(s) 228, 472
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 799, 1059
BtsI GCAGTG 2 cut(s) 501, 1344
BtsIMutI CAGTG 4 cut(s) 501, 1182, 1282, 1344
Cac8I GCNNGC 3 cut(s) 578, 731, 1522
CaiI CAGNNNCTG 1 cut(s) 626
CfoI GCGC 2 cut(s) 168, 1526
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 5 cut(s) 79, 207, 280, 322, 550
ClaI ATCGAT 2 cut(s) 228, 472
CseI GACGC 3 cut(s) 157, 323, 1442
Csp6I GTAC 2 cut(s) 890, 1309
CspCI CAANNNNNGTGG 4 cut(s) 258, 293, 1333, 1368
CviAII CATG 8 cut(s) 331, 365, 370, 571, 730, 1262, 1301, 1565
CviQI GTAC 2 cut(s) 890, 1309
DdeI CTNAG 5 cut(s) 249, 1188, 1420, 1515, 1583
DpnI GATC 7 cut(s) 56, 182, 231, 261, 471, 1113, 1440
DpnII GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
DraI TTTAAA 1 cut(s) 672
Eam1104I CTCTTC 1 cut(s) 141
EarI CTCTTC 1 cut(s) 141
EciI GGCGGA 1 cut(s) 428
Ecl136II GAGCTC 1 cut(s) 40
Eco130I CCWWGG 1 cut(s) 318
Eco147I AGGCCT 2 cut(s) 832, 1520
Eco24I GRGCYC 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 225
Eco47I GGWCC 4 cut(s) 79, 207, 280, 322
Eco53kI GAGCTC 1 cut(s) 40
Eco57I CTGAAG 4 cut(s) 287, 306, 1298, 1524
EcoICRI GAGCTC 1 cut(s) 40
EcoNI CCTNNNNNAGG 1 cut(s) 237
EcoO109I RGGNCCY 1 cut(s) 550
EcoRI GAATTC 2 cut(s) 1245, 1447
EcoRII CCWGG 4 cut(s) 636, 646, 876, 1228
EcoT14I CCWWGG 1 cut(s) 318
EcoT38I GRGCYC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 8 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1568
FalI AAGNNNNNCTT 2 cut(s) 1002, 1034
FaqI GGGAC 4 cut(s) 266, 1157, 1180, 1211
FatI CATG 8 cut(s) 330, 364, 369, 570, 729, 1261, 1300, 1564
FbaI TGATCA 2 cut(s) 54, 1438
Fnu4HI GCNGC 6 cut(s) 455, 480, 627, 1461, 1464, 1562
FokI GGATG 2 cut(s) 786, 1066
FriOI GRGCYC 1 cut(s) 42
Fsp4HI GCNGC 6 cut(s) 455, 480, 627, 1461, 1464, 1562
FspBI CTAG 2 cut(s) 863, 1382
FspI TGCGCA 1 cut(s) 1525
GlaI GCGC 2 cut(s) 167, 1525
GluI GCNGC 6 cut(s) 455, 480, 627, 1461, 1464, 1562
GsaI CCCAGC 1 cut(s) 70
GsuI CTGGAG 1 cut(s) 531
HaeIII GGCC 3 cut(s) 552, 832, 1520
HapII CCGG 4 cut(s) 95, 110, 120, 1270
HgaI GACGC 3 cut(s) 157, 323, 1442
HhaI GCGC 2 cut(s) 168, 1526
Hin1I GRCGYC 1 cut(s) 315
Hin1II CATG 8 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1568
Hin6I GCGC 2 cut(s) 166, 1524
HinP1I GCGC 2 cut(s) 166, 1524
HindIII AAGCTT 1 cut(s) 212
HinfI GANTC 7 cut(s) 175, 251, 866, 1057, 1181, 1224, 1336
HpaII CCGG 4 cut(s) 95, 110, 120, 1270
HphI GGTGA 5 cut(s) 851, 1172, 1244, 1495, 1532
Hpy166II GTNNAC 5 cut(s) 79, 607, 644, 1324, 1540
Hpy188I TCNGA 4 cut(s) 180, 207, 1423, 1504
Hpy8I GTNNAC 5 cut(s) 79, 607, 644, 1324, 1540
HpyAV CCTTC 4 cut(s) 243, 281, 563, 1285
HpyCH4III ACNGT 4 cut(s) 925, 938, 1171, 1537
HpyCH4IV ACGT 2 cut(s) 75, 200
HpyCH4V TGCA 8 cut(s) 457, 482, 580, 620, 629, 644, 733, 914
HpyF10VI GCNNNNNNNGC 4 cut(s) 314, 626, 739, 1570
HpyF3I CTNAG 5 cut(s) 249, 1188, 1420, 1515, 1583
HpySE526I ACGT 2 cut(s) 75, 200
Hsp92I GRCGYC 1 cut(s) 315
Hsp92II CATG 8 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1568
HspAI GCGC 2 cut(s) 166, 1524
Kpn2I TCCGGA 1 cut(s) 1269
Ksp22I TGATCA 2 cut(s) 54, 1438
Kzo9I GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
LmnI GCTCC 5 cut(s) 37, 163, 726, 1511, 1570
Lsp1109I GCAGC 4 cut(s) 441, 466, 613, 1573
LweI GCATC 1 cut(s) 1588
MaeI CTAG 2 cut(s) 863, 1382
MaeII ACGT 2 cut(s) 75, 200
MaeIII GTNAC 3 cut(s) 655, 1105, 1387
MalI GATC 7 cut(s) 56, 182, 231, 261, 471, 1113, 1440
MboI GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
MboII GAAGA 1 cut(s) 158
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 2 cut(s) 259, 1111
MhlI GDGCHC 5 cut(s) 42, 646, 755, 1239, 1260
MlyI GAGTC 3 cut(s) 260, 875, 1233
MmeI TCCRAC 1 cut(s) 1201
MroI TCCGGA 1 cut(s) 1269
MroXI GAANNNNTTC 1 cut(s) 566
MseI TTAA 5 cut(s) 671, 900, 960, 1032, 1203
MslI CAYNNNNRTG 1 cut(s) 585
MspA1I CMGCKG 1 cut(s) 1466
MspI CCGG 4 cut(s) 95, 110, 120, 1270
MspR9I CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
MunI CAATTG 1 cut(s) 309
Mva1269I GAATGC 2 cut(s) 1430, 1582
MvaI CCWGG 4 cut(s) 638, 648, 878, 1230
MvnI CGCG 2 cut(s) 137, 168
MwoI GCNNNNNNNGC 4 cut(s) 314, 626, 739, 1570
NciI CCSGG 2 cut(s) 95, 111
NdeII GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
NlaIII CATG 8 cut(s) 334, 368, 373, 574, 733, 1265, 1304, 1568
NlaIV GGNNCC 6 cut(s) 81, 108, 261, 282, 324, 551
NmuCI GTSAC 1 cut(s) 1105
NsbI TGCGCA 1 cut(s) 1525
NspI RCATGY 2 cut(s) 733, 1568
PaeI GCATGC 1 cut(s) 733
PceI AGGCCT 2 cut(s) 832, 1520
PctI GAATGC 2 cut(s) 1430, 1582
PdmI GAANNNNTTC 1 cut(s) 566
PfeI GAWTC 4 cut(s) 175, 1057, 1181, 1336
PflFI GACNNNGTC 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 216
PkrI GCNGC 6 cut(s) 456, 481, 628, 1462, 1465, 1563
PleI GAGTC 3 cut(s) 259, 874, 1232
PpsI GAGTC 3 cut(s) 259, 874, 1232
Ppu21I YACGTR 1 cut(s) 76
PsiI TTATAA 1 cut(s) 933
Psp124BI GAGCTC 1 cut(s) 42
Psp6I CCWGG 4 cut(s) 636, 646, 876, 1228
PspFI CCCAGC 1 cut(s) 66
PspGI CCWGG 4 cut(s) 636, 646, 876, 1228
PspN4I GGNNCC 6 cut(s) 81, 108, 261, 282, 324, 551
PspPI GGNCC 5 cut(s) 79, 207, 280, 322, 550
PsrI GAACNNNNNNTAC 2 cut(s) 106, 138
PstI CTGCAG 1 cut(s) 459
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 2 cut(s) 259, 1111
PsyI GACNNNGTC 1 cut(s) 332
RsaI GTAC 2 cut(s) 891, 1310
RsaNI GTAC 2 cut(s) 890, 1309
RseI CAYNNNNRTG 1 cut(s) 585
SacI GAGCTC 1 cut(s) 42
SaqAI TTAA 5 cut(s) 671, 900, 960, 1032, 1203
SatI GCNGC 6 cut(s) 455, 480, 627, 1461, 1464, 1562
Sau3AI GATC 7 cut(s) 54, 180, 229, 259, 469, 1111, 1438
Sau96I GGNCC 5 cut(s) 79, 207, 280, 322, 550
SchI GAGTC 3 cut(s) 260, 875, 1233
ScrFI CCNGG 6 cut(s) 95, 111, 638, 648, 878, 1230
SduI GDGCHC 5 cut(s) 42, 646, 755, 1239, 1260
SfaNI GCATC 1 cut(s) 1588
SfcI CTRYAG 1 cut(s) 455
SinI GGWCC 4 cut(s) 79, 207, 280, 322
SmiMI CAYNNNNRTG 1 cut(s) 585
SmlI CTYRAG 2 cut(s) 1008, 1026
SmoI CTYRAG 2 cut(s) 1008, 1026
SphI GCATGC 1 cut(s) 733
SseBI AGGCCT 2 cut(s) 832, 1520
SsiI CCGC 6 cut(s) 137, 158, 439, 806, 1461, 1464
SspMI CTAG 2 cut(s) 863, 1382
SstI GAGCTC 1 cut(s) 42
StuI AGGCCT 2 cut(s) 832, 1520
StyD4I CCNGG 6 cut(s) 93, 109, 636, 646, 876, 1228
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 4 cut(s) 925, 938, 1171, 1537
TaiI ACGT 2 cut(s) 78, 203
TaqI TCGA 6 cut(s) 90, 141, 228, 472, 1062, 1596
TaqII GACCGA 1 cut(s) 115
TauI GCSGC 2 cut(s) 1463, 1466
TfiI GAWTC 4 cut(s) 175, 1057, 1181, 1336
Tru1I TTAA 5 cut(s) 671, 900, 960, 1032, 1203
Tru9I TTAA 5 cut(s) 671, 900, 960, 1032, 1203
TscAI CASTG 4 cut(s) 501, 1182, 1289, 1351
TseFI GTSAC 1 cut(s) 1105
TseI GCWGC 4 cut(s) 454, 479, 626, 1561
Tsp45I GTSAC 1 cut(s) 1105
TspDTI ATGAA 6 cut(s) 159, 559, 1002, 1070, 1107, 1485
TspGWI ACGGA 1 cut(s) 1486
TspRI CASTG 4 cut(s) 501, 1182, 1289, 1351
Tth111I GACNNNGTC 1 cut(s) 332
Van91I CCANNNNNTGG 1 cut(s) 216
VneI GTGCAC 1 cut(s) 642
VpaK11BI GGWCC 4 cut(s) 79, 207, 280, 322
XagI CCTNNNNNAGG 1 cut(s) 237
XapI RAATTY 4 cut(s) 706, 1034, 1245, 1447
XbaI TCTAGA 1 cut(s) 1381
XceI RCATGY 2 cut(s) 733, 1568
XcmI CCANNNNNNNNNTGG 1 cut(s) 644
XmnI GAANNNNTTC 1 cut(s) 566
XspI CTAG 2 cut(s) 863, 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.