pycom10g19960

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0022520
Physical Location & Seq
Forward (+)
22977853 .. 22979016
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g19960.1

Sequence Viewer

Length: 1164 bp
ATGAGTTTGAAGGAGAAATTTTGGATCGATCTTGGAGACTTGGCGCAAGGAATTGCTCAGATGGATAAGGTATTTATAGGAGGAGATTTAAATGGACACATGGGCAAGGAGACAGGCAACTTTGGAGGTTTTCATGGTGGCCATGGTTTTGGGGAGAGAAACGAGGATGGGGAAACCATCTTGGATTTTGCAATGGCATATGATCTCTTCTTAGCCAACACCTTCTTTAAGAAGAGAGAAGAACATGTGATCACCTACAAGAGTGGGTTGTCAAAAACACAAATAGATTTTCTTCTAATGAGGAAGGAGGATCGTATAACTTGTAAGGATTGCAAAGTTATACCGGGAGAGAGCTTGACTAATCAACATTGCTTGTTGATGATGGATGTACATATCAAAAGAGAGAGAAAAAAGAACAAGACCTTAAAGTGCCCAAGGACTAGATGGTGGAATCTAAAAGGAGAAAAACAAGCATTTTTCAAAGAGAAAGTAATCACCCAATGCGTGTGGGATAGAGAGGGGGAAGCTAGCCAAATGTGGGATTCCATGGCTAGTTGTATCCGAAAAGTAGCAAAAGAGGTATTAGGAGAGTCCAAGGGCTTTGCTCCACACCAAAAGGAGTCTTGGTGGTGGAATGAGGAGGTACAAACAAGGGTTAAGGCTAAGAAGGAATGTTGTAAAGCCTTATACAAGGATAGGACCGATGAAAACGGTGAAAGGTATAGAATAACGAAGCAGGAGGCGAAGAAAGCTGTGAGAGAAGCTAAGCTAGCGGCTTATGACGATATGTATAAGCGACTAAATACCAAAGAAGGAGAGTTGGATATCTATAAACTAGCTAGAGTAAGGGAAAAGAAGACAAGGGACCTAAACCAAGTGAGGTGCATCAAGGATGAGAATGGAAAGGTTCTTGCTACAGAGAACGCAGTCAAAGACAGATGGAGAGCTTATTTTCATAATCTTTTCAACGAAGGGCATGAAAGGAGCACTTCTTTGAGAGAGTTGAGTAACTCAGAAAAGTGTAGAAACTACTCCTTTTACTGTCGAATTAGAAAGGAAGAAATGGTTGTAACTTTGAAAAAGATGAAGCATAGAAAAGCAGTGGGCCCAGATGATATACCGATTGAAGTGGGGAAAGTCTTGGGAAAGACGGGTATAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

388

Amino Acids

45.13

Weight (kDa)

9.29

Isoelectric Point (pI)

25.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0022520)

Species Orthologous Gene IDs
pyrus_communis pycom01g03100 pycom10g19960 pycom11g12410 pycom15g29570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 773
AclWI GGATC 2 cut(s) 32, 318
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 1 cut(s) 17
AfaI GTAC 2 cut(s) 390, 645
AfiI CCNNNNNNNGG 1 cut(s) 538
AflIII ACRYGT 1 cut(s) 244
AgsI TTSAA 5 cut(s) 10, 481, 967, 1078, 1127
AluBI AGCT 7 cut(s) 354, 527, 752, 764, 769, 839, 947
AluI AGCT 7 cut(s) 354, 527, 752, 764, 769, 839, 947
Alw21I GWGCWC 1 cut(s) 989
Alw26I GTCTC 2 cut(s) 30, 104
AlwI GGATC 2 cut(s) 32, 318
AoxI GGCC 2 cut(s) 139, 1105
ApaI GGGCCC 1 cut(s) 1109
ApoI RAATTY 1 cut(s) 17
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 4 cut(s) 699, 865, 1105, 1106
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 3 cut(s) 244, 487, 725
AsuNHI GCTAGC 2 cut(s) 527, 769
AvaII GGWCC 2 cut(s) 699, 865
BaeGI GKGCMC 2 cut(s) 434, 1109
BalI TGGCCA 1 cut(s) 141
BanII GRGCYC 1 cut(s) 1109
BbsI GAAGAC 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 989
BccI CCATC 6 cut(s) 55, 161, 185, 376, 438, 933
BciVI GTATCC 1 cut(s) 569
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 1 cut(s) 345
BcoDI GTCTC 2 cut(s) 30, 104
BfaI CTAG 6 cut(s) 441, 528, 552, 770, 836, 840
BfmI CTRYAG 1 cut(s) 915
BfuI GTATCC 1 cut(s) 569
BisI GCNGC 1 cut(s) 774
BlpI GCTNAGC 1 cut(s) 765
BlsI GCNGC 1 cut(s) 775
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 2 cut(s) 699, 865
BmgT120I GGNCC 4 cut(s) 699, 865, 1105, 1106
BmiI GGNNCC 2 cut(s) 866, 1107
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 894
BmtI GCTAGC 2 cut(s) 531, 773
BpiI GAAGAC 1 cut(s) 863
Bpu1102I GCTNAGC 1 cut(s) 765
BpuMI CCSGG 1 cut(s) 345
Bsa29I ATCGAT 1 cut(s) 27
BsaJI CCNNGG 4 cut(s) 142, 434, 546, 594
BsaXI ACNNNNNCTCC 2 cut(s) 611, 641
Bsc4I CCNNNNNNNGG 1 cut(s) 538
Bse3DI GCAATG 2 cut(s) 198, 367
BseCI ATCGAT 1 cut(s) 27
BseDI CCNNGG 4 cut(s) 142, 434, 546, 594
BseGI GGATG 3 cut(s) 172, 391, 898
BseLI CCNNNNNNNGG 1 cut(s) 538
BseMI GCAATG 2 cut(s) 198, 367
BseMII CTCAG 2 cut(s) 71, 1026
BseRI GAGGAG 2 cut(s) 96, 653
BseSI GKGCMC 2 cut(s) 434, 1109
BshFI GGCC 2 cut(s) 141, 1107
BshVI ATCGAT 1 cut(s) 27
BsiHKAI GWGCWC 1 cut(s) 989
BsiSI CCGG 1 cut(s) 344
BslFI GGGAC 1 cut(s) 878
BslI CCNNNNNNNGG 1 cut(s) 538
BsmAI GTCTC 2 cut(s) 30, 104
BsmFI GGGAC 1 cut(s) 878
BsnI GGCC 2 cut(s) 141, 1107
Bsp120I GGGCCC 1 cut(s) 1105
Bsp1286I GDGCHC 3 cut(s) 434, 989, 1109
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 5 cut(s) 24, 28, 202, 249, 310
Bsp1720I GCTNAGC 1 cut(s) 765
Bsp19I CCATGG 2 cut(s) 142, 546
BspACI CCGC 1 cut(s) 773
BspANI GGCC 2 cut(s) 141, 1107
BspCNI CTCAG 2 cut(s) 70, 1025
BspDI ATCGAT 1 cut(s) 27
BspLI GGNNCC 2 cut(s) 866, 1107
BspOI GCTAGC 2 cut(s) 531, 773
BspPI GGATC 2 cut(s) 32, 318
BsrDI GCAATG 2 cut(s) 198, 367
BsrGI TGTACA 1 cut(s) 388
BssECI CCNNGG 4 cut(s) 142, 434, 546, 594
BssMI GATC 5 cut(s) 24, 28, 202, 249, 310
BssT1I CCWWGG 4 cut(s) 142, 434, 546, 594
Bst4CI ACNGT 2 cut(s) 713, 1043
Bst6I CTCTTC 2 cut(s) 212, 227
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 2 cut(s) 529, 771
BstDEI CTNAG 5 cut(s) 57, 211, 663, 765, 1012
BstDSI CCRYGG 2 cut(s) 142, 546
BstF5I GGATG 3 cut(s) 172, 391, 898
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 5 cut(s) 27, 31, 205, 252, 313
BstMAI GTCTC 2 cut(s) 30, 104
BstMBI GATC 5 cut(s) 24, 28, 202, 249, 310
BstMWI GCNNNNNNNGC 2 cut(s) 749, 770
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 915
BstSLI GKGCMC 2 cut(s) 434, 1109
BstV2I GAAGAC 1 cut(s) 863
BstXI CCANNNNNNTGG 1 cut(s) 149
Bsu15I ATCGAT 1 cut(s) 27
BsuI GTATCC 1 cut(s) 569
BsuRI GGCC 2 cut(s) 141, 1107
BsuTUI ATCGAT 1 cut(s) 27
BtgI CCRYGG 2 cut(s) 142, 546
BtsCI GGATG 3 cut(s) 172, 391, 898
BtsI GCAGTG 1 cut(s) 1107
BtsIMutI CAGTG 1 cut(s) 1107
Cac8I GCNNGC 2 cut(s) 529, 771
CfoI GCGC 1 cut(s) 46
Cfr13I GGNCC 4 cut(s) 699, 865, 1105, 1106
ClaI ATCGAT 1 cut(s) 27
Csp6I GTAC 2 cut(s) 389, 644
CspCI CAANNNNNGTGG 2 cut(s) 488, 523
CviAII CATG 7 cut(s) 100, 134, 143, 245, 547, 977, 1161
CviQI GTAC 2 cut(s) 389, 644
DdeI CTNAG 5 cut(s) 57, 211, 663, 765, 1012
DpnI GATC 5 cut(s) 26, 30, 204, 251, 312
DpnII GATC 5 cut(s) 24, 28, 202, 249, 310
DraI TTTAAA 1 cut(s) 90
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 2 cut(s) 212, 227
EarI CTCTTC 2 cut(s) 212, 227
Eco130I CCWWGG 4 cut(s) 142, 434, 546, 594
Eco24I GRGCYC 1 cut(s) 1109
Eco32I GATATC 1 cut(s) 826
Eco47I GGWCC 2 cut(s) 699, 865
EcoO109I RGGNCCY 1 cut(s) 865
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 4 cut(s) 142, 434, 546, 594
EcoT38I GRGCYC 1 cut(s) 1109
ErhI CCWWGG 4 cut(s) 142, 434, 546, 594
FaeI CATG 7 cut(s) 103, 137, 146, 248, 550, 980, 1164
FalI AAGNNNNNCTT 2 cut(s) 973, 1005
FaqI GGGAC 1 cut(s) 878
FatI CATG 7 cut(s) 99, 133, 142, 244, 546, 976, 1160
FauNDI CATATG 1 cut(s) 199
FbaI TGATCA 1 cut(s) 249
Fnu4HI GCNGC 1 cut(s) 774
FokI GGATG 3 cut(s) 179, 398, 905
FriOI GRGCYC 1 cut(s) 1109
Fsp4HI GCNGC 1 cut(s) 774
FspBI CTAG 6 cut(s) 441, 528, 552, 770, 836, 840
GlaI GCGC 1 cut(s) 45
GluI GCNGC 1 cut(s) 774
HaeIII GGCC 2 cut(s) 141, 1107
HapII CCGG 1 cut(s) 344
HhaI GCGC 1 cut(s) 46
Hin1II CATG 7 cut(s) 103, 137, 146, 248, 550, 980, 1164
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HinfI GANTC 4 cut(s) 451, 542, 590, 620
HpaII CCGG 1 cut(s) 344
HphI GGTGA 3 cut(s) 244, 487, 725
Hpy188I TCNGA 3 cut(s) 60, 563, 1015
HpyAV CCTTC 6 cut(s) 4, 232, 298, 661, 806, 965
HpyCH4III ACNGT 2 cut(s) 713, 1043
HpyCH4V TGCA 3 cut(s) 191, 333, 885
HpyF10VI GCNNNNNNNGC 2 cut(s) 749, 770
HpyF3I CTNAG 5 cut(s) 57, 211, 663, 765, 1012
Hsp92II CATG 7 cut(s) 103, 137, 146, 248, 550, 980, 1164
HspAI GCGC 1 cut(s) 44
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 5 cut(s) 24, 28, 202, 249, 310
LmnI GCTCC 2 cut(s) 610, 984
LpnPI CCDG 4 cut(s) 99, 357, 722, 1122
LweI GCATC 1 cut(s) 894
MaeI CTAG 6 cut(s) 441, 528, 552, 770, 836, 840
MaeIII GTNAC 2 cut(s) 1007, 1069
MalI GATC 5 cut(s) 26, 30, 204, 251, 312
MboI GATC 5 cut(s) 24, 28, 202, 249, 310
MboII GAAGA 7 cut(s) 199, 244, 251, 284, 757, 868, 1070
MhlI GDGCHC 3 cut(s) 434, 989, 1109
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 3 cut(s) 17, 51, 1047
MluNI TGGCCA 1 cut(s) 141
MlyI GAGTC 2 cut(s) 599, 629
MmeI TCCRAC 1 cut(s) 801
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 4 cut(s) 89, 228, 425, 657
Msp20I TGGCCA 1 cut(s) 141
MspI CCGG 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 345
MwoI GCNNNNNNNGC 2 cut(s) 749, 770
NciI CCSGG 1 cut(s) 345
NcoI CCATGG 2 cut(s) 142, 546
NdeI CATATG 1 cut(s) 199
NdeII GATC 5 cut(s) 24, 28, 202, 249, 310
NheI GCTAGC 2 cut(s) 527, 769
NlaIII CATG 7 cut(s) 103, 137, 146, 248, 550, 980, 1164
NlaIV GGNNCC 2 cut(s) 866, 1107
NspI RCATGY 1 cut(s) 248
PciI ACATGT 1 cut(s) 244
PfeI GAWTC 2 cut(s) 451, 542
PkrI GCNGC 1 cut(s) 775
PleI GAGTC 2 cut(s) 598, 628
PpsI GAGTC 2 cut(s) 598, 628
PpuMI RGGWCCY 1 cut(s) 865
PscI ACATGT 1 cut(s) 244
Psp5II RGGWCCY 1 cut(s) 865
PspN4I GGNNCC 2 cut(s) 866, 1107
PspOMI GGGCCC 1 cut(s) 1105
PspPI GGNCC 4 cut(s) 699, 865, 1105, 1106
PspPPI RGGWCCY 1 cut(s) 865
RsaI GTAC 2 cut(s) 390, 645
RsaNI GTAC 2 cut(s) 389, 644
SaqAI TTAA 4 cut(s) 89, 228, 425, 657
SatI GCNGC 1 cut(s) 774
Sau3AI GATC 5 cut(s) 24, 28, 202, 249, 310
Sau96I GGNCC 4 cut(s) 699, 865, 1105, 1106
SchI GAGTC 2 cut(s) 599, 629
ScrFI CCNGG 1 cut(s) 345
SduI GDGCHC 3 cut(s) 434, 989, 1109
SfaNI GCATC 1 cut(s) 894
SfcI CTRYAG 1 cut(s) 915
SinI GGWCC 2 cut(s) 699, 865
SmiI ATTTAAAT 1 cut(s) 90
Sse9I AATT 3 cut(s) 17, 51, 1047
SsiI CCGC 1 cut(s) 773
SspMI CTAG 6 cut(s) 441, 528, 552, 770, 836, 840
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 4 cut(s) 142, 434, 546, 594
SwaI ATTTAAAT 1 cut(s) 90
TaaI ACNGT 2 cut(s) 713, 1043
TaqI TCGA 2 cut(s) 27, 1045
TaqII GACCGA 1 cut(s) 716
TasI AATT 3 cut(s) 17, 51, 1047
TatI WGTACW 1 cut(s) 388
TauI GCSGC 1 cut(s) 776
TfiI GAWTC 2 cut(s) 451, 542
Tru1I TTAA 4 cut(s) 89, 228, 425, 657
Tru9I TTAA 4 cut(s) 89, 228, 425, 657
TscAI CASTG 1 cut(s) 1107
TspDTI ATGAA 5 cut(s) 122, 720, 944, 993, 1100
TspRI CASTG 1 cut(s) 1107
VpaK11BI GGWCC 2 cut(s) 699, 865
XapI RAATTY 1 cut(s) 17
XceI RCATGY 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 441
XspI CTAG 6 cut(s) 441, 528, 552, 770, 836, 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.