pycom10g20720

Wall-associated receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0024393
Physical Location & Seq
Forward (+)
23561050 .. 23561409
360 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g20720.1

Sequence Viewer

Length: 360 bp
ATGGGAACCTGCATAAATACAGCGGGAAATTATACGTGTAAATGTCCGAAGGGGTTCAAAAATGACGATCTGGATAAAAAAAGTTGCATCAAGGACAACGGTCGAGCAAAGCTCAGTCTCCCGCTTATTATTTCACTAGGTATGCATATGTTAGTTTTATTTTACTTTTTCTTTAGGCGCATGTTTTTGCATATACAATTAGAGCTGATTCGGGTCGGGATCCCGAACCAAAACTCCCAAACCAAAATATTTCAGGATGGAATTTATATCCCAAACCAAACTGAAACTGTTCATTTCCCGAAATTTGGGATTCCCGAAACTGTTTCGGGATTTTCGGGTCACCTCGGGATTGGGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EGF_CA GUB_WAK_bind Pkinase Pkinase_Tyr WAK

Protein Analysis

120

Amino Acids

13.38

Weight (kDa)

9.18

Isoelectric Point (pI)

24.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024393)

Species Orthologous Gene IDs
malus_domestica MD08G1212400.v1.1
pyrus_communis pycom10g20720 pycom15g26900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 17
AciI CCGC 2 cut(s) 23, 122
AclWI GGATC 2 cut(s) 214, 227
AcsI RAATTY 2 cut(s) 261, 302
AfiI CCNNNNNNNGG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 35
AgsI TTSAA 1 cut(s) 58
AloI GAACNNNNNNTCC 2 cut(s) 218, 250
AluBI AGCT 2 cut(s) 112, 205
AluI AGCT 2 cut(s) 112, 205
Alw26I GTCTC 1 cut(s) 122
AlwI GGATC 2 cut(s) 214, 227
Ama87I CYCGRG 1 cut(s) 344
AoxI GGCC 1 cut(s) 353
ApoI RAATTY 2 cut(s) 261, 302
Asp700I GAANNNNTTC 2 cut(s) 53, 288
AspLEI GCGC 1 cut(s) 180
AspS9I GGNCC 1 cut(s) 353
AsuHPI GGTGA 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 344
BamHI GGATCC 1 cut(s) 219
BccI CCATC 1 cut(s) 251
BcoDI GTCTC 1 cut(s) 122
BfaI CTAG 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 17
BmeT110I CYCGRG 1 cut(s) 344
BmgT120I GGNCC 1 cut(s) 353
BmiI GGNNCC 2 cut(s) 7, 221
BmsI GCATC 1 cut(s) 96
BoxI GACNNNNGTC 1 cut(s) 99
BplI GAGNNNNNCTC 2 cut(s) 96, 128
BsaAI YACGTR 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 343
BsaXI ACNNNNNCTCC 2 cut(s) 218, 248
Bsc4I CCNNNNNNNGG 1 cut(s) 305
BseDI CCNNGG 1 cut(s) 343
BseGI GGATG 1 cut(s) 262
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMII CTCAG 1 cut(s) 127
Bsh1285I CGRYCG 1 cut(s) 103
BshFI GGCC 1 cut(s) 355
BsiEI CGRYCG 1 cut(s) 103
BsiHKCI CYCGRG 1 cut(s) 344
BslI CCNNNNNNNGG 1 cut(s) 305
BsmAI GTCTC 1 cut(s) 122
BsnI GGCC 1 cut(s) 355
BsoBI CYCGRG 1 cut(s) 344
Bsp143I GATC 2 cut(s) 67, 219
BspACI CCGC 2 cut(s) 23, 122
BspANI GGCC 1 cut(s) 355
BspCNI CTCAG 1 cut(s) 126
BspLI GGNNCC 2 cut(s) 7, 221
BspMI ACCTGC 1 cut(s) 17
BspPI GGATC 2 cut(s) 214, 227
BssECI CCNNGG 1 cut(s) 343
BssMI GATC 2 cut(s) 67, 219
Bst4CI ACNGT 3 cut(s) 101, 289, 322
BstBAI YACGTR 1 cut(s) 36
BstDEI CTNAG 1 cut(s) 113
BstEII GGTNACC 1 cut(s) 338
BstF5I GGATG 1 cut(s) 262
BstHHI GCGC 1 cut(s) 180
BstKTI GATC 2 cut(s) 70, 222
BstMAI GTCTC 1 cut(s) 122
BstMBI GATC 2 cut(s) 67, 219
BstMCI CGRYCG 1 cut(s) 103
BstNSI RCATGY 1 cut(s) 184
BstPAI GACNNNNGTC 1 cut(s) 99
BstPI GGTNACC 1 cut(s) 338
BstX2I RGATCY 1 cut(s) 219
BstYI RGATCY 1 cut(s) 219
BsuRI GGCC 1 cut(s) 355
BtsCI GGATG 1 cut(s) 262
BveI ACCTGC 1 cut(s) 17
CfoI GCGC 1 cut(s) 180
Cfr13I GGNCC 1 cut(s) 353
CviAII CATG 1 cut(s) 181
CviJI RGCY 3 cut(s) 112, 205, 355
CviKI_1 RGCY 3 cut(s) 112, 205, 355
DdeI CTNAG 1 cut(s) 113
DpnI GATC 2 cut(s) 69, 221
DpnII GATC 2 cut(s) 67, 219
Eco88I CYCGRG 1 cut(s) 344
Eco91I GGTNACC 1 cut(s) 338
EcoO65I GGTNACC 1 cut(s) 338
EcoT22I ATGCAT 1 cut(s) 147
FaeI CATG 1 cut(s) 184
FaiI YATR 9 cut(s) 14, 33, 143, 147, 149, 182, 192, 194, 267
FatI CATG 1 cut(s) 180
FauI CCCGC 2 cut(s) 16, 129
FauNDI CATATG 1 cut(s) 147
FokI GGATG 1 cut(s) 269
FspBI CTAG 1 cut(s) 137
GlaI GCGC 1 cut(s) 179
HaeIII GGCC 1 cut(s) 355
HhaI GCGC 1 cut(s) 180
Hin1II CATG 1 cut(s) 184
Hin6I GCGC 1 cut(s) 178
HinP1I GCGC 1 cut(s) 178
HinfI GANTC 2 cut(s) 208, 310
HphI GGTGA 1 cut(s) 332
Hpy188I TCNGA 1 cut(s) 48
Hpy188III TCNNGA 8 cut(s) 71, 217, 223, 254, 298, 314, 327, 346
HpyAV CCTTC 1 cut(s) 43
HpyCH4III ACNGT 3 cut(s) 101, 289, 322
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 4 cut(s) 12, 87, 145, 190
HpyF3I CTNAG 1 cut(s) 113
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 1 cut(s) 184
HspAI GCGC 1 cut(s) 178
Kzo9I GATC 2 cut(s) 67, 219
LpnPI CCDG 3 cut(s) 22, 56, 239
LweI GCATC 1 cut(s) 96
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 1 cut(s) 338
MalI GATC 2 cut(s) 69, 221
MboI GATC 2 cut(s) 67, 219
MflI RGATCY 1 cut(s) 219
MluCI AATT 4 cut(s) 28, 197, 261, 302
MnlI CCTC 1 cut(s) 353
Mph1103I ATGCAT 1 cut(s) 147
MroXI GAANNNNTTC 2 cut(s) 53, 288
MspA1I CMGCKG 1 cut(s) 23
NdeI CATATG 1 cut(s) 147
NdeII GATC 2 cut(s) 67, 219
NlaIII CATG 1 cut(s) 184
NlaIV GGNNCC 2 cut(s) 7, 221
NmuCI GTSAC 1 cut(s) 338
NsiI ATGCAT 1 cut(s) 147
NspI RCATGY 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 53, 288
PfeI GAWTC 2 cut(s) 208, 310
Ppu21I YACGTR 1 cut(s) 36
PshAI GACNNNNGTC 1 cut(s) 99
PspEI GGTNACC 1 cut(s) 338
PspN4I GGNNCC 2 cut(s) 7, 221
PspPI GGNCC 1 cut(s) 353
PsuI RGATCY 1 cut(s) 219
Sau3AI GATC 2 cut(s) 67, 219
Sau96I GGNCC 1 cut(s) 353
SetI ASST 6 cut(s) 11, 38, 114, 142, 207, 345
SfaNI GCATC 1 cut(s) 96
Sse9I AATT 4 cut(s) 28, 197, 261, 302
SsiI CCGC 2 cut(s) 23, 122
SspI AATATT 1 cut(s) 249
SspMI CTAG 1 cut(s) 137
TaaI ACNGT 3 cut(s) 101, 289, 322
TaiI ACGT 1 cut(s) 38
TaqI TCGA 1 cut(s) 103
TasI AATT 4 cut(s) 28, 197, 261, 302
TfiI GAWTC 2 cut(s) 208, 310
TseFI GTSAC 1 cut(s) 338
Tsp45I GTSAC 1 cut(s) 338
TspDTI ATGAA 1 cut(s) 281
XapI RAATTY 2 cut(s) 261, 302
XceI RCATGY 1 cut(s) 184
XmnI GAANNNNTTC 2 cut(s) 53, 288
XspI CTAG 1 cut(s) 137
Zsp2I ATGCAT 1 cut(s) 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.