pycom10g22210

CAS1 domain-containing protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0024127
Physical Location & Seq
Reverse (-)
24635926 .. 24637993
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGGTGGACGCTCGGCCAGTTACGCCTGGACAGGTGTCATTTTTGCTTGGAATCATCCCAGTTTTTGTCACATGGATATATTCAGAGTACTTGGAGTATAAAAAATCTTCAATTCCTCCTAAAGTTCATTCAGATACTAATCTTGTTGAGTTGGGAGAGGAGACAATCAAGGAAGATGATCGAGCTGTTTTGCTGGAAGGAGGCCTTTCAAGATCAGCATCTACAAAGTTTAATAGTTCATCCATCAAAATAAATTTAATCAGGTTTGTGAATATGGATGACTCTTTTCTACTTGAAAACAGAGCAACTCTTAGAGTGATGTCTGAGCTTGGGGCAATTCTGTTCTATTTTTTCACATGTGACCGCACAAATATACTGGGAGATTCTACGAAGAAATACAATCGGGATCTTTTCATCTGTCCTGTACCTTATCCTTGTCATAGTTTCAGCAATGACATCTTTGAAAAAACATACTGA

Protein Analysis

159

Amino Acids

18.02

Weight (kDa)

5.4

Isoelectric Point (pI)

38.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cas1_AcylT PF07779 101 - 144 3.9e-06 10 TM Acyl Transferase domain found in Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024127)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g20470
pyrus_communis pycom10g22210
rosa_samantha Rh4CG283000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 364
AclWI GGATC 1 cut(s) 414
AcoI YGGCCR 1 cut(s) 14
AcsI RAATTY 1 cut(s) 253
AfaI GTAC 2 cut(s) 89, 426
AflIII ACRYGT 1 cut(s) 356
AgsI TTSAA 4 cut(s) 111, 210, 296, 464
AjnI CCWGG 1 cut(s) 25
AluBI AGCT 2 cut(s) 185, 328
AluI AGCT 2 cut(s) 185, 328
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 1 cut(s) 414
AoxI GGCC 2 cut(s) 14, 202
ApoI RAATTY 1 cut(s) 253
BccI CCATC 1 cut(s) 251
BciT130I CCWGG 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 155
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 27
BmrFI CCNGG 1 cut(s) 27
BmrI ACTGGG 2 cut(s) 53, 386
BmsI GCATC 1 cut(s) 227
BmuI ACTGGG 2 cut(s) 53, 386
BoxI GACNNNNGTC 1 cut(s) 34
BsaBI GATNNNNATC 2 cut(s) 138, 217
Bse1I ACTGG 3 cut(s) 17, 59, 381
Bse3DI GCAATG 1 cut(s) 457
Bse8I GATNNNNATC 2 cut(s) 138, 217
BseBI CCWGG 1 cut(s) 27
BseGI GGATG 3 cut(s) 54, 239, 283
BseJI GATNNNNATC 2 cut(s) 138, 217
BseMI GCAATG 1 cut(s) 457
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 3 cut(s) 17, 59, 381
BseRI GAGGAG 1 cut(s) 173
BshFI GGCC 2 cut(s) 16, 204
BsmAI GTCTC 1 cut(s) 155
BsnI GGCC 2 cut(s) 16, 204
Bsp143I GATC 3 cut(s) 178, 212, 406
BspACI CCGC 1 cut(s) 364
BspANI GGCC 2 cut(s) 16, 204
BspCNI CTCAG 1 cut(s) 316
BspPI GGATC 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 457
BsrI ACTGG 3 cut(s) 17, 59, 381
BssMI GATC 3 cut(s) 178, 212, 406
Bst2UI CCWGG 1 cut(s) 27
BstDEI CTNAG 2 cut(s) 311, 324
BstF5I GGATG 3 cut(s) 54, 239, 283
BstKTI GATC 3 cut(s) 181, 215, 409
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 3 cut(s) 178, 212, 406
BstMWI GCNNNNNNNGC 1 cut(s) 22
BstNI CCWGG 1 cut(s) 27
BstNSI RCATGY 1 cut(s) 360
BstPAI GACNNNNGTC 1 cut(s) 34
BstSCI CCNGG 1 cut(s) 25
BstX2I RGATCY 1 cut(s) 406
BstYI RGATCY 1 cut(s) 406
BsuRI GGCC 2 cut(s) 16, 204
BtsCI GGATG 3 cut(s) 54, 239, 283
CseI GACGC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 88, 425
CviAII CATG 2 cut(s) 72, 357
CviJI RGCY 4 cut(s) 16, 185, 204, 328
CviKI_1 RGCY 4 cut(s) 16, 185, 204, 328
CviQI GTAC 2 cut(s) 88, 425
DdeI CTNAG 2 cut(s) 311, 324
DpnI GATC 3 cut(s) 180, 214, 408
DpnII GATC 3 cut(s) 178, 212, 406
EaeI YGGCCR 1 cut(s) 14
Eco147I AGGCCT 1 cut(s) 204
EcoRII CCWGG 1 cut(s) 25
FaeI CATG 2 cut(s) 75, 360
FaiI YATR 8 cut(s) 73, 79, 99, 275, 358, 374, 441, 472
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FatI CATG 2 cut(s) 71, 356
FokI GGATG 3 cut(s) 41, 226, 290
HaeIII GGCC 2 cut(s) 16, 204
HgaI GACGC 1 cut(s) 17
Hin1II CATG 2 cut(s) 75, 360
HinfI GANTC 3 cut(s) 51, 281, 383
Hpy166II GTNNAC 1 cut(s) 7
Hpy188I TCNGA 3 cut(s) 85, 133, 325
Hpy188III TCNNGA 2 cut(s) 210, 404
Hpy8I GTNNAC 1 cut(s) 7
HpyAV CCTTC 1 cut(s) 191
HpyF10VI GCNNNNNNNGC 1 cut(s) 22
HpyF3I CTNAG 2 cut(s) 311, 324
Hsp92II CATG 2 cut(s) 75, 360
Kzo9I GATC 3 cut(s) 178, 212, 406
LpnPI CCDG 9 cut(s) 12, 17, 30, 39, 72, 179, 247, 362, 435
LweI GCATC 1 cut(s) 227
MaeIII GTNAC 3 cut(s) 19, 67, 359
MalI GATC 3 cut(s) 180, 214, 408
MboI GATC 3 cut(s) 178, 212, 406
MboII GAAGA 3 cut(s) 99, 185, 403
MflI RGATCY 1 cut(s) 406
MluCI AATT 3 cut(s) 111, 253, 336
MlyI GAGTC 1 cut(s) 275
MnlI CCTC 3 cut(s) 126, 151, 194
MseI TTAA 2 cut(s) 231, 257
MspR9I CCNGG 1 cut(s) 27
MvaI CCWGG 1 cut(s) 27
MwoI GCNNNNNNNGC 1 cut(s) 22
NdeII GATC 3 cut(s) 178, 212, 406
NlaIII CATG 2 cut(s) 75, 360
NmuCI GTSAC 2 cut(s) 67, 359
NspI RCATGY 1 cut(s) 360
PceI AGGCCT 1 cut(s) 204
PciI ACATGT 1 cut(s) 356
PfeI GAWTC 2 cut(s) 51, 383
PleI GAGTC 1 cut(s) 275
PpsI GAGTC 1 cut(s) 275
PscI ACATGT 1 cut(s) 356
PshAI GACNNNNGTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 25
PspGI CCWGG 1 cut(s) 25
PsuI RGATCY 1 cut(s) 406
RsaI GTAC 2 cut(s) 89, 426
RsaNI GTAC 2 cut(s) 88, 425
SaqAI TTAA 2 cut(s) 231, 257
Sau3AI GATC 3 cut(s) 178, 212, 406
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 1 cut(s) 275
ScrFI CCNGG 1 cut(s) 27
SetI ASST 5 cut(s) 36, 187, 266, 330, 430
SfaNI GCATC 1 cut(s) 227
Sse9I AATT 3 cut(s) 111, 253, 336
SseBI AGGCCT 1 cut(s) 204
SsiI CCGC 1 cut(s) 364
StuI AGGCCT 1 cut(s) 204
StyD4I CCNGG 1 cut(s) 25
TaqI TCGA 1 cut(s) 181
TasI AATT 3 cut(s) 111, 253, 336
TatI WGTACW 1 cut(s) 87
TfiI GAWTC 2 cut(s) 51, 383
Tru1I TTAA 2 cut(s) 231, 257
Tru9I TTAA 2 cut(s) 231, 257
TseFI GTSAC 2 cut(s) 67, 359
Tsp45I GTSAC 2 cut(s) 67, 359
TspDTI ATGAA 3 cut(s) 116, 228, 403
XapI RAATTY 1 cut(s) 253
XceI RCATGY 1 cut(s) 360
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.