pycom10g26700

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0020822
Physical Location & Seq
Forward (+)
27956562 .. 27957935
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1146 bp
ATGCAAATCTTCGTGAAAACTCTTACCGGCAAGACCATCACCCTCGAGGTGGAGTCATCTGACACCATCGACAACGTCAAGGCCAAGATCGAGGACAAGGAAGGGATCCCCCCAGACCAGCAGCGTCTGATCTTCGCCGGAAAGCAGCTCGAAGATGGCCGAACCCTTGCAGACTACAATATCCAGAAGGAGTCAACCCTCCACTTGGTGCTCCGTCTTCGCGGTGGAATGCAGATCTTTGTTAAGACCCTCACCGGCAAGACCATCACCCTCGAGGTGGAGTCATCCGACACCATCGACAACGTCAAGGCCAAGATCCAAGACAAGGAAGGGATTCCCCCAGACCAGCAGCGTCTGATTTTCGCCGGAAAGCAGCTTGAAGATGGCCGTACCCTTGCAGATTACAACATCCAGAAAGAGTCAACCCTCCACTTGGTGCTCCGTCTTCGTGGTGGCATGCAGATCTTCGTTAAGACCCTCACCGGGAAGACCATCACCCTTGAGGTGGAATCATCCGATACCATTGACAATGTCAAGGCCAAAATCCAAGACAAGGAGGGCATCCCTCCAGACCAGCAGAGGCTTATCTTTGCTGGAAAGCAGCTTGAGGACGGCCGCACCCTTGCCGACTACAACATCCAGAAGGAGTCCACTCTCCATTTGGTGTTGCGTCTCCGTGGTGGCATGCAGATTTTCGTCAAGACCCTGACAGGAAAGACCATCACCCTGGAGGTCGAGAGTTCGGACACCATTGACAATGTCAAGGCCAAGATCCAGGACAAGGAGGGAATCCCACCGGACCAGCAGAGGCTGATCTTTGCCGGGAAGCAGCTTGAGGATGGCCGCACTCTGGCGGACTACAACATCCAGAAGGAGTCAACCCTTCACTTGGTGCTGCGTCTGAGGGGTGGTATGCAGATATTCGTAAAGACCTTGACAGGAAAGACCATCACCTTGGAGGTGGAGAGCTCTGACACCATCGACAATGTGAAGGCAAAGATCCAGGACAAGGAAGGCATCCCTCCGGACCAGCAGAGGTTGATTTTTGCTGGAAAGCAGTTGGAGGATGGAAGGACCCTGGCTGATTACAACATTCAGAAGGAGTCCACCCTTCACCTTGTCCTCCGTCTCCGTGGTGGTTTTTAA

Protein Analysis

382

Amino Acids

42.7

Weight (kDa)

6.58

Isoelectric Point (pI)

30.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rad60-SLD PF11976 1 - 71 1.5e-16 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 3 - 74 1.2e-31 Ubiquitin family
Rad60-SLD PF11976 77 - 147 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 79 - 150 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 153 - 223 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 155 - 226 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 229 - 299 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 231 - 302 1.1e-31 Ubiquitin family
Rad60-SLD PF11976 305 - 375 8.5e-17 Ubiquitin-2 like Rad60 SUMO-like
ubiquitin PF00240 307 - 378 1.1e-31 Ubiquitin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020822)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G37640
malus_domestica MD11G1226200.v1.1
prunus_persica Prupe.4G204900_v2.0.a1 Prupe.4G204900_v2.0.a1
pyrus_communis pycom10g26700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 2 cut(s) 44, 272
AccII CGCG 1 cut(s) 222
AccIII TCCGGA 1 cut(s) 1024
AciI CCGC 4 cut(s) 222, 616, 844, 854
AclWI GGATC 5 cut(s) 100, 113, 310, 766, 994
AcoI YGGCCR 4 cut(s) 157, 385, 613, 841
AdeI CACNNNGTG 3 cut(s) 208, 436, 892
AfaI GTAC 1 cut(s) 391
AgsI TTSAA 1 cut(s) 380
AjnI CCWGG 4 cut(s) 726, 774, 1002, 1077
AluBI AGCT 5 cut(s) 148, 376, 604, 832, 969
AluI AGCT 5 cut(s) 148, 376, 604, 832, 969
Alw21I GWGCWC 3 cut(s) 213, 441, 971
Alw26I GTCTC 2 cut(s) 677, 1133
AlwI GGATC 5 cut(s) 100, 113, 310, 766, 994
AlwNI CAGNNNCTG 3 cut(s) 127, 355, 811
Ama87I CYCGRG 2 cut(s) 44, 272
Aor13HI TCCGGA 1 cut(s) 1024
AoxI GGCC 8 cut(s) 81, 157, 309, 385, 537, 613, 765, 841
ApeKI GCWGC 7 cut(s) 121, 145, 349, 373, 601, 829, 895
Asp700I GAANNNNTTC 1 cut(s) 333
AspS9I GGNCC 3 cut(s) 799, 1027, 1074
AsuC2I CCSGG 2 cut(s) 484, 823
AsuHPI GGTGA 8 cut(s) 31, 244, 259, 472, 487, 715, 943, 1106
AvaI CYCGRG 2 cut(s) 44, 272
AvaII GGWCC 3 cut(s) 799, 1027, 1074
BamHI GGATCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 971
BbsI GAAGAC 3 cut(s) 209, 437, 494
Bbv12I GWGCWC 3 cut(s) 213, 441, 971
BbvI GCAGC 7 cut(s) 133, 157, 361, 385, 613, 841, 882
BceAI ACGGC 2 cut(s) 372, 628
BciT130I CCWGG 4 cut(s) 728, 776, 1004, 1079
BcnI CCSGG 2 cut(s) 484, 823
BcoDI GTCTC 2 cut(s) 677, 1133
BglII AGATCT 2 cut(s) 234, 462
BisI GCNGC 9 cut(s) 122, 146, 350, 374, 602, 616, 830, 844, 896
BlsI GCNGC 9 cut(s) 123, 147, 351, 375, 603, 617, 831, 845, 897
Bme1390I CCNGG 6 cut(s) 484, 728, 776, 823, 1004, 1079
Bme18I GGWCC 3 cut(s) 799, 1027, 1074
BmeT110I CYCGRG 2 cut(s) 44, 272
BmgT120I GGNCC 3 cut(s) 799, 1027, 1074
BmiI GGNNCC 2 cut(s) 107, 1076
BmrFI CCNGG 6 cut(s) 484, 728, 776, 823, 1004, 1079
BmsI GCATC 2 cut(s) 570, 1026
BpiI GAAGAC 3 cut(s) 209, 437, 494
BpmI CTGGAG 2 cut(s) 552, 749
BpuEI CTTGAG 3 cut(s) 521, 626, 854
BpuMI CCSGG 2 cut(s) 484, 823
BsaJI CCNNGG 5 cut(s) 676, 726, 954, 1077, 1132
BsaWI WCCGGW 2 cut(s) 796, 1024
Bse118I RCCGGY 2 cut(s) 26, 254
BseAI TCCGGA 1 cut(s) 1024
BseBI CCWGG 4 cut(s) 728, 776, 1004, 1079
BseDI CCNNGG 5 cut(s) 676, 726, 954, 1077, 1132
BseGI GGATG 9 cut(s) 284, 408, 512, 561, 636, 844, 864, 1017, 1072
BseMII CTCAG 1 cut(s) 893
BseX3I CGGCCG 1 cut(s) 613
BseXI GCAGC 7 cut(s) 133, 157, 361, 385, 613, 841, 882
Bsh1236I CGCG 1 cut(s) 222
Bsh1285I CGRYCG 1 cut(s) 616
BshFI GGCC 8 cut(s) 83, 159, 311, 387, 539, 615, 767, 843
BsiEI CGRYCG 1 cut(s) 616
BsiHKAI GWGCWC 3 cut(s) 213, 441, 971
BsiHKCI CYCGRG 2 cut(s) 44, 272
BsiSI CCGG 8 cut(s) 27, 138, 255, 366, 483, 797, 822, 1025
BsmAI GTCTC 2 cut(s) 677, 1133
BsmBI CGTCTC 2 cut(s) 677, 1133
BsmI GAATGC 1 cut(s) 234
BsnI GGCC 8 cut(s) 83, 159, 311, 387, 539, 615, 767, 843
BsoBI CYCGRG 2 cut(s) 44, 272
Bsp1286I GDGCHC 3 cut(s) 213, 441, 971
Bsp13I TCCGGA 1 cut(s) 1024
Bsp143I GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
BspACI CCGC 4 cut(s) 222, 616, 844, 854
BspANI GGCC 8 cut(s) 83, 159, 311, 387, 539, 615, 767, 843
BspCNI CTCAG 1 cut(s) 894
BspEI TCCGGA 1 cut(s) 1024
BspFNI CGCG 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 107, 1076
BspPI GGATC 5 cut(s) 100, 113, 310, 766, 994
BsrFI RCCGGY 2 cut(s) 26, 254
BssAI RCCGGY 2 cut(s) 26, 254
BssECI CCNNGG 5 cut(s) 676, 726, 954, 1077, 1132
BssMI GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
BssT1I CCWWGG 1 cut(s) 954
Bst2UI CCWGG 4 cut(s) 728, 776, 1004, 1079
BstC8I GCNNGC 2 cut(s) 458, 686
BstDEI CTNAG 1 cut(s) 902
BstDSI CCRYGG 2 cut(s) 676, 1132
BstF5I GGATG 9 cut(s) 284, 408, 512, 561, 636, 844, 864, 1017, 1072
BstFNI CGCG 1 cut(s) 222
BstKTI GATC 9 cut(s) 90, 108, 132, 237, 318, 465, 774, 816, 1002
BstMAI GTCTC 2 cut(s) 677, 1133
BstMBI GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
BstMCI CGRYCG 1 cut(s) 616
BstNI CCWGG 4 cut(s) 728, 776, 1004, 1079
BstNSI RCATGY 2 cut(s) 460, 688
BstSCI CCNGG 6 cut(s) 482, 726, 774, 821, 1002, 1077
BstUI CGCG 1 cut(s) 222
BstV1I GCAGC 7 cut(s) 133, 157, 361, 385, 613, 841, 882
BstV2I GAAGAC 3 cut(s) 209, 437, 494
BstX2I RGATCY 6 cut(s) 105, 234, 315, 462, 771, 999
BstXI CCANNNNNNTGG 2 cut(s) 727, 955
BstYI RGATCY 6 cut(s) 105, 234, 315, 462, 771, 999
BstZI CGGCCG 1 cut(s) 613
BsuRI GGCC 8 cut(s) 83, 159, 311, 387, 539, 615, 767, 843
BtgI CCRYGG 2 cut(s) 676, 1132
BtsCI GGATG 9 cut(s) 284, 408, 512, 561, 636, 844, 864, 1017, 1072
Cac8I GCNNGC 2 cut(s) 458, 686
CaiI CAGNNNCTG 3 cut(s) 127, 355, 811
Cfr10I RCCGGY 2 cut(s) 26, 254
Cfr13I GGNCC 3 cut(s) 799, 1027, 1074
CseI GACGC 4 cut(s) 113, 341, 659, 887
Csp6I GTAC 1 cut(s) 390
CviAII CATG 2 cut(s) 457, 685
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 1 cut(s) 902
DpnI GATC 9 cut(s) 89, 107, 131, 236, 317, 464, 773, 815, 1001
DpnII GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
DraIII CACNNNGTG 3 cut(s) 208, 436, 892
EaeI YGGCCR 4 cut(s) 157, 385, 613, 841
EagI CGGCCG 1 cut(s) 613
EciI GGCGGA 1 cut(s) 869
Ecl136II GAGCTC 1 cut(s) 969
EclXI CGGCCG 1 cut(s) 613
Eco130I CCWWGG 1 cut(s) 954
Eco24I GRGCYC 1 cut(s) 971
Eco47I GGWCC 3 cut(s) 799, 1027, 1074
Eco52I CGGCCG 1 cut(s) 613
Eco53kI GAGCTC 1 cut(s) 969
Eco88I CYCGRG 2 cut(s) 44, 272
EcoICRI GAGCTC 1 cut(s) 969
EcoO109I RGGNCCY 1 cut(s) 1074
EcoRII CCWGG 4 cut(s) 726, 774, 1002, 1077
EcoT14I CCWWGG 1 cut(s) 954
EcoT38I GRGCYC 1 cut(s) 971
ErhI CCWWGG 1 cut(s) 954
Esp3I CGTCTC 2 cut(s) 677, 1133
FaeI CATG 2 cut(s) 460, 688
FaiI YATR 3 cut(s) 458, 686, 914
FatI CATG 2 cut(s) 456, 684
Fnu4HI GCNGC 9 cut(s) 122, 146, 350, 374, 602, 616, 830, 844, 896
FokI GGATG 9 cut(s) 271, 395, 499, 548, 623, 851, 851, 1004, 1079
FriOI GRGCYC 1 cut(s) 971
Fsp4HI GCNGC 9 cut(s) 122, 146, 350, 374, 602, 616, 830, 844, 896
GluI GCNGC 9 cut(s) 122, 146, 350, 374, 602, 616, 830, 844, 896
GsuI CTGGAG 2 cut(s) 552, 749
HaeIII GGCC 8 cut(s) 83, 159, 311, 387, 539, 615, 767, 843
HapII CCGG 8 cut(s) 27, 138, 255, 366, 483, 797, 822, 1025
HgaI GACGC 4 cut(s) 113, 341, 659, 887
Hin1II CATG 2 cut(s) 460, 688
HincII GTYRAC 3 cut(s) 195, 423, 879
HindII GTYRAC 3 cut(s) 195, 423, 879
HpaII CCGG 8 cut(s) 27, 138, 255, 366, 483, 797, 822, 1025
HphI GGTGA 8 cut(s) 31, 244, 259, 472, 487, 715, 943, 1106
Hpy166II GTNNAC 5 cut(s) 195, 423, 651, 879, 1107
Hpy188I TCNGA 9 cut(s) 61, 129, 289, 357, 517, 745, 903, 973, 1098
Hpy188III TCNNGA 9 cut(s) 13, 184, 412, 569, 640, 700, 736, 868, 1025
Hpy8I GTNNAC 5 cut(s) 195, 423, 651, 879, 1107
HpyCH4IV ACGT 2 cut(s) 75, 303
HpyCH4V TGCA 7 cut(s) 4, 170, 232, 398, 460, 688, 916
HpyF3I CTNAG 1 cut(s) 902
HpySE526I ACGT 2 cut(s) 75, 303
Hsp92II CATG 2 cut(s) 460, 688
Kpn2I TCCGGA 1 cut(s) 1024
Kzo9I GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
LmnI GCTCC 2 cut(s) 216, 444
Lsp1109I GCAGC 7 cut(s) 133, 157, 361, 385, 613, 841, 882
LweI GCATC 2 cut(s) 570, 1026
MaeII ACGT 2 cut(s) 75, 303
MalI GATC 9 cut(s) 89, 107, 131, 236, 317, 464, 773, 815, 1001
MboI GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
MboII GAAGA 7 cut(s) 124, 164, 209, 392, 437, 457, 499
MflI RGATCY 6 cut(s) 105, 234, 315, 462, 771, 999
MhlI GDGCHC 3 cut(s) 213, 441, 971
MlyI GAGTC 7 cut(s) 62, 200, 290, 428, 656, 884, 1112
MmeI TCCRAC 2 cut(s) 312, 1041
MroI TCCGGA 1 cut(s) 1024
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 3 cut(s) 243, 471, 1144
MspI CCGG 8 cut(s) 27, 138, 255, 366, 483, 797, 822, 1025
MspR9I CCNGG 6 cut(s) 484, 728, 776, 823, 1004, 1079
Mva1269I GAATGC 1 cut(s) 234
MvaI CCWGG 4 cut(s) 728, 776, 1004, 1079
MvnI CGCG 1 cut(s) 222
NciI CCSGG 2 cut(s) 484, 823
NdeII GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
NlaIII CATG 2 cut(s) 460, 688
NlaIV GGNNCC 2 cut(s) 107, 1076
NspI RCATGY 2 cut(s) 460, 688
PaeI GCATGC 2 cut(s) 460, 688
PaeR7I CTCGAG 2 cut(s) 44, 272
PctI GAATGC 1 cut(s) 234
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 3 cut(s) 334, 509, 789
PflFI GACNNNGTC 4 cut(s) 74, 302, 530, 758
PfoI TCCNGGA 2 cut(s) 774, 1002
PkrI GCNGC 9 cut(s) 123, 147, 351, 375, 603, 617, 831, 845, 897
PleI GAGTC 7 cut(s) 61, 199, 289, 427, 655, 883, 1111
PpsI GAGTC 7 cut(s) 61, 199, 289, 427, 655, 883, 1111
PpuMI RGGWCCY 1 cut(s) 1074
Psp124BI GAGCTC 1 cut(s) 971
Psp5II RGGWCCY 1 cut(s) 1074
Psp6I CCWGG 4 cut(s) 726, 774, 1002, 1077
PspGI CCWGG 4 cut(s) 726, 774, 1002, 1077
PspN4I GGNNCC 2 cut(s) 107, 1076
PspPI GGNCC 3 cut(s) 799, 1027, 1074
PspPPI RGGWCCY 1 cut(s) 1074
PspXI VCTCGAGB 2 cut(s) 44, 272
PstNI CAGNNNCTG 3 cut(s) 127, 355, 811
PsuI RGATCY 6 cut(s) 105, 234, 315, 462, 771, 999
PsyI GACNNNGTC 4 cut(s) 74, 302, 530, 758
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
SacI GAGCTC 1 cut(s) 971
SaqAI TTAA 3 cut(s) 243, 471, 1144
SatI GCNGC 9 cut(s) 122, 146, 350, 374, 602, 616, 830, 844, 896
Sau3AI GATC 9 cut(s) 87, 105, 129, 234, 315, 462, 771, 813, 999
Sau96I GGNCC 3 cut(s) 799, 1027, 1074
SchI GAGTC 7 cut(s) 62, 200, 290, 428, 656, 884, 1112
ScrFI CCNGG 6 cut(s) 484, 728, 776, 823, 1004, 1079
SduI GDGCHC 3 cut(s) 213, 441, 971
SfaNI GCATC 2 cut(s) 570, 1026
Sfr274I CTCGAG 2 cut(s) 44, 272
SinI GGWCC 3 cut(s) 799, 1027, 1074
SlaI CTCGAG 2 cut(s) 44, 272
SmlI CTYRAG 5 cut(s) 44, 272, 500, 605, 833
SmoI CTYRAG 5 cut(s) 44, 272, 500, 605, 833
SphI GCATGC 2 cut(s) 460, 688
SsiI CCGC 4 cut(s) 222, 616, 844, 854
SstI GAGCTC 1 cut(s) 971
StyD4I CCNGG 6 cut(s) 482, 726, 774, 821, 1002, 1077
StyI CCWWGG 1 cut(s) 954
TaiI ACGT 2 cut(s) 78, 306
TaqI TCGA 8 cut(s) 45, 69, 90, 150, 273, 297, 735, 981
TauI GCSGC 2 cut(s) 618, 846
TfiI GAWTC 3 cut(s) 334, 509, 789
Tru1I TTAA 3 cut(s) 243, 471, 1144
Tru9I TTAA 3 cut(s) 243, 471, 1144
TseI GCWGC 7 cut(s) 121, 145, 349, 373, 601, 829, 895
TspGWI ACGGA 5 cut(s) 203, 431, 665, 1115, 1121
Tth111I GACNNNGTC 4 cut(s) 74, 302, 530, 758
VpaK11BI GGWCC 3 cut(s) 799, 1027, 1074
XceI RCATGY 2 cut(s) 460, 688
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XhoI CTCGAG 2 cut(s) 44, 272
XmnI GAANNNNTTC 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.