pycom10g28540

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0019170
Physical Location & Seq
Forward (+)
29108188 .. 29110060
1873 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1635 bp
ATGGTAGGTATTTCAGGATCAAGAATGAAGGCCCTCCTCATCTCCTCCGTCCCTCACATCTCAAAGACCATACCTTTATCTTTAGCCTCCTCCTCCTCCTCCTCCTCCAAGTCCTTCTCCTTCAGCCCTCCTACTCTTCTCCAGAACCCCAATATTCTCCTCCCAAAACCCAATATTTTTCTCCCCAAAAGCTCCATTTCTTCAGCTCAGAACATCCGATGTGTGCAAATGGAGGCAGAATCTCACCAGCGCTCCGGTGAGATTCACGTCATCGTCGGCCCCATGTTCGCCGGAAAGACCACCACGCTTCTTCGTAAAGTTCAGTCCGAGAGAGGCGATGGCAGCTTTCAGGTCAAGCCTCAAAAGCACATCCAACTGGATAAAACACTCAAGGGACTCTGCTATTCGGGAAGATTGGCAGAAGCTGTTGCGCTATTGTGCCGCACAGGGTTAGAGTTCAACCCCGCCACCTATGCTCTTCTATTGCAAGAATGCATATTTAGGAAAGAGTATAAGAAGGGGAAAAGAATCCATGCACATATGATTGTTGTGGGATTTTTTCTCAATGAATATTTGAAGATCAAGTTGTTGATTTTGTATGCAAAATCAGGGGATTTAAGGACTGCCCACATTCTGCTTGATATGTTGCTTGAGAAAAGCTTGGTTTCATGGAACGCAATAATTGCAGGGTATGTGCAAAAGGGGCTTGAAGATGTGGGGTTGAGTCTTTATAACAAGATGAGACAGAGGGGTTTGATCCCAGACCAGTATACCTTTGCATCGGTCTTCAGAGCGTGTGCATCTTTAGCAACACTGGAGCATGGGAAGCAAGCTCATGGTGTCATGACAAAGTGCCAAATTGGACAAAATGTTGTAGTCAGTAGTGCCCTCATGGATATGTACTTTAAATGTAGTGATATATCTGACGGGCATAGGGTATTTGATACATCTCAAAACAGGAATGCCATTACCTGGACTGCTTTGATATCTGGGTATGGTCAGCATGGAAGAGTTGTAGAGGTCTTGCATCTTTTTCATAGGATGAAGAGTGAAGGTTTCAGGCCAAACTATGTTACTTTTATCTCAGTTCTTTCTGCTTGCAGTCATGGAGCTTTAGTTGACGAGGCTTGGGAATACTTTTCTTCCATGACAAGAGATTATGGAATTCGGCCAAGAGCACAACATTATGCAGCCTTGGTAGACGTTTTAGGACGTGTTGGGCGGTTAGAAGAAGCCTATGAGTTTGTTTCAAACTCACCTTATAAGGAGCACTCAGTGATATGGGGTGCTTTTCTTTGGGCTTGTAGAATTCATGGAGATAGGGACTTGTTAAAGCTTGCAGCAAAGAAGTACTTCACATTGGAACCAGAGAATGCTGGGAAGTATGTGGTCTTGTCAAATGCTTATGCCACTTTTGGGTTGTGGGATAATGTTGCTAAGGTTAGGGATATGATGAAGGAATCAGGGATAGTAAAGGAGCCCGCTTATAGCAAAATAGAGGTTCAAAAGGAGGTCCATTTCTTTCTTATGGGTGATACATATCACAAACAATCCAAACAGATTTATGAAATGGCTAAATTGGTTGATTTTTCTCTAAAGGGTTCAGCCTTATGCTTCTCAGTAAGGGGAAGCTAA

Protein Analysis

545

Amino Acids

60.88

Weight (kDa)

9.38

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 220 - 267 2.4e-11 PPR repeat family
PPR PF01535 222 - 252 7.2e-06 PPR repeat
PPR_2 PF13041 321 - 368 3.1e-11 PPR repeat family
PPR PF01535 323 - 353 7e-09 PPR repeat
E_motif PF20431 438 - 500 9.9e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019170)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G16470
fragaria_vesca FvH4_3g00700
prunus_persica Prupe.4G008000_v2.0.a1
pyrus_communis pycom10g28540
rosa_laevigata RLG00000030908
rosa_multiflora Rmu_sc0019885.1_g000004
rosa_roxburghii Rroxscaffold_1G00075320
rosa_rugosa Rorug04G0388000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 732, 1263
AccB7I CCANNNNNTGG 1 cut(s) 972
AccI GTMKAC 2 cut(s) 770, 1200
AciI CCGC 4 cut(s) 442, 465, 1222, 1482
AclWI GGATC 2 cut(s) 25, 751
AcoI YGGCCR 1 cut(s) 1169
AcsI RAATTY 2 cut(s) 1164, 1308
AcuI CTGAAG 3 cut(s) 106, 186, 772
AdeI CACNNNGTG 1 cut(s) 1276
AfaI GTAC 2 cut(s) 902, 1352
AfeI AGCGCT 1 cut(s) 251
AfiI CCNNNNNNNGG 2 cut(s) 972, 1416
AflIII ACRYGT 1 cut(s) 1213
AgsI TTSAA 5 cut(s) 460, 577, 710, 1251, 1505
AjiI CACGTC 2 cut(s) 268, 1214
AjnI CCWGG 1 cut(s) 971
AluBI AGCT 9 cut(s) 192, 206, 345, 425, 660, 833, 1112, 1336, 1632
AluI AGCT 9 cut(s) 192, 206, 345, 425, 660, 833, 1112, 1336, 1632
Alw21I GWGCWC 2 cut(s) 1180, 1272
Alw26I GTCTC 1 cut(s) 736
AlwI GGATC 2 cut(s) 25, 751
AlwNI CAGNNNCTG 1 cut(s) 425
Aor51HI AGCGCT 1 cut(s) 251
AoxI GGCC 4 cut(s) 30, 277, 1061, 1169
ApeKI GCWGC 3 cut(s) 342, 1190, 1340
ApoI RAATTY 2 cut(s) 1164, 1308
Asp700I GAANNNNTTC 1 cut(s) 1352
AspLEI GCGC 2 cut(s) 252, 433
AspS9I GGNCC 3 cut(s) 31, 278, 1513
AsuHPI GGTGA 4 cut(s) 236, 269, 1248, 1544
AvaII GGWCC 1 cut(s) 1513
BaeGI GKGCMC 1 cut(s) 889
BanII GRGCYC 1 cut(s) 1482
BbsI GAAGAC 1 cut(s) 778
Bbv12I GWGCWC 2 cut(s) 1180, 1272
BbvI GCAGC 3 cut(s) 354, 1202, 1352
BccI CCATC 1 cut(s) 332
BciT130I CCWGG 1 cut(s) 973
BcoDI GTCTC 1 cut(s) 736
BfoI RGCGCY 1 cut(s) 253
BisI GCNGC 4 cut(s) 343, 442, 1191, 1341
BlsI GCNGC 4 cut(s) 344, 443, 1192, 1342
BmcAI AGTACT 1 cut(s) 1352
Bme1390I CCNGG 1 cut(s) 973
Bme18I GGWCC 1 cut(s) 1513
BmgBI CACGTC 2 cut(s) 268, 1214
BmgT120I GGNCC 3 cut(s) 31, 278, 1513
BmiI GGNNCC 3 cut(s) 280, 1365, 1479
BmrFI CCNGG 1 cut(s) 973
BmsI GCATC 3 cut(s) 788, 809, 1036
BpiI GAAGAC 1 cut(s) 778
BpmI CTGGAG 2 cut(s) 125, 836
Bpu10I CCTNAGC 1 cut(s) 1437
BpuEI CTTGAG 2 cut(s) 374, 671
BsaBI GATNNNNATC 1 cut(s) 1539
BsaJI CCNNGG 1 cut(s) 1194
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 1101, 1131
Bsc4I CCNNNNNNNGG 2 cut(s) 972, 1416
Bse1I ACTGG 3 cut(s) 381, 766, 819
Bse8I GATNNNNATC 1 cut(s) 1539
BseBI CCWGG 1 cut(s) 973
BseDI CCNNGG 1 cut(s) 1194
BseGI GGATG 3 cut(s) 213, 369, 1047
BseJI GATNNNNATC 1 cut(s) 1539
BseLI CCNNNNNNNGG 2 cut(s) 972, 1416
BseMII CTCAG 4 cut(s) 221, 1098, 1287, 1632
BseNI ACTGG 3 cut(s) 381, 766, 819
BseRI GAGGAG 9 cut(s) 26, 34, 79, 82, 85, 88, 91, 94, 149
BseSI GKGCMC 1 cut(s) 889
BseXI GCAGC 3 cut(s) 354, 1202, 1352
BseYI CCCAGC 1 cut(s) 1376
BshFI GGCC 4 cut(s) 32, 279, 1063, 1171
BsiHKAI GWGCWC 2 cut(s) 1180, 1272
BsiSI CCGG 2 cut(s) 255, 291
BslFI GGGAC 3 cut(s) 35, 408, 1337
BslI CCNNNNNNNGG 2 cut(s) 972, 1416
BsmAI GTCTC 1 cut(s) 736
BsmFI GGGAC 3 cut(s) 35, 408, 1337
BsmI GAATGC 3 cut(s) 497, 967, 1378
BsnI GGCC 4 cut(s) 32, 279, 1063, 1171
Bsp1286I GDGCHC 4 cut(s) 889, 1180, 1272, 1482
Bsp143I GATC 3 cut(s) 17, 579, 756
BspACI CCGC 4 cut(s) 442, 465, 1222, 1482
BspANI GGCC 4 cut(s) 32, 279, 1063, 1171
BspCNI CTCAG 4 cut(s) 220, 1097, 1286, 1631
BspHI TCATGA 1 cut(s) 843
BspLI GGNNCC 3 cut(s) 280, 1365, 1479
BspPI GGATC 2 cut(s) 25, 751
BspQI GCTCTTC 1 cut(s) 483
BsrI ACTGG 3 cut(s) 381, 766, 819
BssECI CCNNGG 1 cut(s) 1194
BssMI GATC 3 cut(s) 17, 579, 756
BssNAI GTATAC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 1194
Bst1107I GTATAC 1 cut(s) 771
Bst2UI CCWGG 1 cut(s) 973
Bst6I CTCTTC 4 cut(s) 141, 483, 1003, 1040
BstAPI GCANNNNNTGC 1 cut(s) 683
BstC8I GCNNGC 4 cut(s) 831, 1099, 1338, 1482
BstDEI CTNAG 5 cut(s) 207, 1084, 1273, 1437, 1618
BstF5I GGATG 3 cut(s) 213, 369, 1047
BstH2I RGCGCY 1 cut(s) 253
BstHHI GCGC 2 cut(s) 252, 433
BstKTI GATC 3 cut(s) 20, 582, 759
BstMAI GTCTC 1 cut(s) 736
BstMBI GATC 3 cut(s) 17, 579, 756
BstMWI GCNNNNNNNGC 7 cut(s) 342, 364, 473, 683, 703, 806, 826
BstNI CCWGG 1 cut(s) 973
BstSCI CCNGG 1 cut(s) 971
BstSLI GKGCMC 1 cut(s) 889
BstV1I GCAGC 3 cut(s) 354, 1202, 1352
BstV2I GAAGAC 1 cut(s) 778
BstZ17I GTATAC 1 cut(s) 771
BsuRI GGCC 4 cut(s) 32, 279, 1063, 1171
BtgZI GCGATG 1 cut(s) 351
BtrI CACGTC 2 cut(s) 268, 1214
BtsCI GGATG 3 cut(s) 213, 369, 1047
BtsIMutI CAGTG 2 cut(s) 812, 1281
Cac8I GCNNGC 4 cut(s) 831, 1099, 1338, 1482
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 843
CfoI GCGC 2 cut(s) 252, 433
Cfr13I GGNCC 3 cut(s) 31, 278, 1513
Csp6I GTAC 2 cut(s) 901, 1351
CviQI GTAC 2 cut(s) 901, 1351
DdeI CTNAG 5 cut(s) 207, 1084, 1273, 1437, 1618
DpnI GATC 3 cut(s) 19, 581, 758
DpnII GATC 3 cut(s) 17, 579, 756
DraI TTTAAA 1 cut(s) 907
DraIII CACNNNGTG 1 cut(s) 1276
EaeI YGGCCR 1 cut(s) 1169
Eam1104I CTCTTC 4 cut(s) 141, 483, 1003, 1040
EarI CTCTTC 4 cut(s) 141, 483, 1003, 1040
Eco130I CCWWGG 1 cut(s) 1194
Eco24I GRGCYC 1 cut(s) 1482
Eco32I GATATC 1 cut(s) 987
Eco47I GGWCC 1 cut(s) 1513
Eco47III AGCGCT 1 cut(s) 251
Eco57I CTGAAG 3 cut(s) 106, 186, 772
EcoO109I RGGNCCY 1 cut(s) 31
EcoRI GAATTC 2 cut(s) 1164, 1308
EcoRII CCWGG 1 cut(s) 971
EcoRV GATATC 1 cut(s) 987
EcoT14I CCWWGG 1 cut(s) 1194
EcoT22I ATGCAT 1 cut(s) 497
EcoT38I GRGCYC 1 cut(s) 1482
ErhI CCWWGG 1 cut(s) 1194
FalI AAGNNNNNCTT 2 cut(s) 1337, 1369
FaqI GGGAC 3 cut(s) 35, 408, 1337
FauI CCCGC 2 cut(s) 472, 1489
FauNDI CATATG 1 cut(s) 540
FblI GTMKAC 2 cut(s) 770, 1200
Fnu4HI GCNGC 4 cut(s) 343, 442, 1191, 1341
FokI GGATG 3 cut(s) 200, 356, 1054
FriOI GRGCYC 1 cut(s) 1482
Fsp4HI GCNGC 4 cut(s) 343, 442, 1191, 1341
GlaI GCGC 2 cut(s) 251, 432
GluI GCNGC 4 cut(s) 343, 442, 1191, 1341
GsaI CCCAGC 1 cut(s) 1380
GsuI CTGGAG 2 cut(s) 125, 836
HaeII RGCGCY 1 cut(s) 253
HaeIII GGCC 4 cut(s) 32, 279, 1063, 1171
HapII CCGG 2 cut(s) 255, 291
HhaI GCGC 2 cut(s) 252, 433
Hin6I GCGC 2 cut(s) 250, 431
HinP1I GCGC 2 cut(s) 250, 431
HincII GTYRAC 1 cut(s) 1120
HindII GTYRAC 1 cut(s) 1120
HindIII AAGCTT 2 cut(s) 658, 1334
HinfI GANTC 6 cut(s) 239, 262, 396, 528, 724, 1460
HpaII CCGG 2 cut(s) 255, 291
HphI GGTGA 4 cut(s) 236, 269, 1248, 1544
Hpy166II GTNNAC 3 cut(s) 771, 1120, 1201
Hpy188I TCNGA 5 cut(s) 210, 218, 328, 791, 925
Hpy188III TCNNGA 5 cut(s) 15, 21, 142, 408, 844
Hpy8I GTNNAC 3 cut(s) 771, 1120, 1201
Hpy99I CGWCG 1 cut(s) 278
HpyAV CCTTC 6 cut(s) 22, 124, 130, 511, 1046, 1450
HpyCH4IV ACGT 3 cut(s) 267, 1203, 1213
HpyF10VI GCNNNNNNNGC 7 cut(s) 342, 364, 473, 683, 703, 806, 826
HpyF3I CTNAG 5 cut(s) 207, 1084, 1273, 1437, 1618
HpySE526I ACGT 3 cut(s) 267, 1203, 1213
HspAI GCGC 2 cut(s) 250, 431
Kzo9I GATC 3 cut(s) 17, 579, 756
LguI GCTCTTC 1 cut(s) 483
LmnI GCTCC 6 cut(s) 197, 257, 817, 1109, 1267, 1477
Lsp1109I GCAGC 3 cut(s) 354, 1202, 1352
LweI GCATC 3 cut(s) 788, 809, 1036
MaeII ACGT 3 cut(s) 267, 1203, 1213
MaeIII GTNAC 1 cut(s) 1072
MalI GATC 3 cut(s) 19, 581, 758
MboI GATC 3 cut(s) 17, 579, 756
MhlI GDGCHC 4 cut(s) 889, 1180, 1272, 1482
MluCI AATT 5 cut(s) 681, 858, 1164, 1308, 1577
MlyI GAGTC 2 cut(s) 390, 733
MmeI TCCRAC 1 cut(s) 397
Mph1103I ATGCAT 1 cut(s) 497
MroXI GAANNNNTTC 1 cut(s) 1352
MseI TTAA 3 cut(s) 617, 906, 1331
MslI CAYNNNNRTG 1 cut(s) 896
MspI CCGG 2 cut(s) 255, 291
MspR9I CCNGG 1 cut(s) 973
Mva1269I GAATGC 3 cut(s) 497, 967, 1378
MvaI CCWGG 1 cut(s) 973
MwoI GCNNNNNNNGC 7 cut(s) 342, 364, 473, 683, 703, 806, 826
NdeI CATATG 1 cut(s) 540
NdeII GATC 3 cut(s) 17, 579, 756
NlaIV GGNNCC 3 cut(s) 280, 1365, 1479
NsiI ATGCAT 1 cut(s) 497
PagI TCATGA 1 cut(s) 843
PciSI GCTCTTC 1 cut(s) 483
PctI GAATGC 3 cut(s) 497, 967, 1378
PdmI GAANNNNTTC 1 cut(s) 1352
PfeI GAWTC 4 cut(s) 239, 262, 528, 1460
PflMI CCANNNNNTGG 1 cut(s) 972
PkrI GCNGC 4 cut(s) 344, 443, 1192, 1342
PleI GAGTC 2 cut(s) 390, 732
PpsI GAGTC 2 cut(s) 390, 732
PsiI TTATAA 2 cut(s) 732, 1263
Psp6I CCWGG 1 cut(s) 971
PspFI CCCAGC 1 cut(s) 1376
PspGI CCWGG 1 cut(s) 971
PspN4I GGNNCC 3 cut(s) 280, 1365, 1479
PspPI GGNCC 3 cut(s) 31, 278, 1513
PstNI CAGNNNCTG 1 cut(s) 425
RsaI GTAC 2 cut(s) 902, 1352
RsaNI GTAC 2 cut(s) 901, 1351
RseI CAYNNNNRTG 1 cut(s) 896
SapI GCTCTTC 1 cut(s) 483
SaqAI TTAA 3 cut(s) 617, 906, 1331
SatI GCNGC 4 cut(s) 343, 442, 1191, 1341
Sau3AI GATC 3 cut(s) 17, 579, 756
Sau96I GGNCC 3 cut(s) 31, 278, 1513
ScaI AGTACT 1 cut(s) 1352
SchI GAGTC 2 cut(s) 390, 733
ScrFI CCNGG 1 cut(s) 973
SduI GDGCHC 4 cut(s) 889, 1180, 1272, 1482
SfaNI GCATC 3 cut(s) 788, 809, 1036
SinI GGWCC 1 cut(s) 1513
SmiMI CAYNNNNRTG 1 cut(s) 896
SmlI CTYRAG 2 cut(s) 389, 650
SmoI CTYRAG 2 cut(s) 389, 650
Sse9I AATT 5 cut(s) 681, 858, 1164, 1308, 1577
SsiI CCGC 4 cut(s) 442, 465, 1222, 1482
SspI AATATT 3 cut(s) 154, 175, 572
StyD4I CCNGG 1 cut(s) 971
StyI CCWWGG 1 cut(s) 1194
TaiI ACGT 3 cut(s) 270, 1206, 1216
TaqII GACCGA 1 cut(s) 772
TasI AATT 5 cut(s) 681, 858, 1164, 1308, 1577
TatI WGTACW 2 cut(s) 900, 1350
TauI GCSGC 1 cut(s) 444
TfiI GAWTC 4 cut(s) 239, 262, 528, 1460
Tru1I TTAA 3 cut(s) 617, 906, 1331
Tru9I TTAA 3 cut(s) 617, 906, 1331
TscAI CASTG 2 cut(s) 819, 1281
TseI GCWGC 3 cut(s) 342, 1190, 1340
TspDTI ATGAA 8 cut(s) 41, 582, 657, 1025, 1058, 1301, 1469, 1581
TspGWI ACGGA 1 cut(s) 37
TspRI CASTG 2 cut(s) 819, 1281
Van91I CCANNNNNTGG 1 cut(s) 972
VpaK11BI GGWCC 1 cut(s) 1513
XapI RAATTY 2 cut(s) 1164, 1308
XmiI GTMKAC 2 cut(s) 770, 1200
XmnI GAANNNNTTC 1 cut(s) 1352
ZrmI AGTACT 1 cut(s) 1352
Zsp2I ATGCAT 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.