pycom10g28650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0021117
Physical Location & Seq
Forward (+)
29169627 .. 29170759
1133 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 894 bp
ATGTTTTCACTTTTCAGAAGGGCCATTCATTCCCTTGTAACCCTAGCTTCGTCACTCACAATATTTGTAAGCAATCACACATACATATGGATCGATCAAAGTAGTGAGGATCAGAAGCGCCAAGCTTTTGACAAATTCATCGACAATAAGTACTACGCCAAGTTGTTTATGACGAGGAATTTGAAGTTTGTAGTCTCAGTGTCTTTGTTGTCCTTGTTTCTTTTTGTAACATCATCAGGTTCTTGTGTACTTCCCGATCCCTTCAATCAGTTCAACGCCTATTTCTCCACATTGCTTTTGTCAATCTTTACCCACACCCTTGAGAGGAAGTACATATTTCTTATTTGTAATGGAATCCTTGCTTTTCTTGCAAAAACTTCGACTAATTCTTGCTGTTGCAGTTGCAATGATGAATCCGATGAGCTTATGTCCGACCTTGGTGGAAAGTCTTCAACATCATCATCAGTTAATTCTAACTTATCTGAGATTGGGTCGTCTCTCGGCCAGGATGGTATCGCTTATGTGGCTGACAAAGGAGAAGAAGAACCAACGTTGACTTCAGACGAAGAAGAAGAAGAAGAAGAAGAAGTTGGACAAGTACAAGATCTAGAAAAACAACAAAATGGTTCTTTATATACAGAGAACCATGAAGAAATGCAGCCTGAAGCTTTCGTTGAAGAAGAAGAAGAAGAAGAAGTGAGTGAGTCTTTGATGAAGCAAGGAGAAGAAGAAACGGAAACACAAGAGCTAGCAACAAATTCTGACGTTGGAGATGATCAGTACGGCTTAACGAGCAGTACAGATGAATTGAACAAGAAAATCGAAGAGTTCATAAGAAAAATGAAGGAGGAAATCAGGATTGAAGCCCAACGCCAACCCATCATTGCCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

298

Amino Acids

33.53

Weight (kDa)

4.31

Isoelectric Point (pI)

61.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021117)

Species Orthologous Gene IDs
malus_domestica MD00G1057200.v1.1 MD10G1337100.v1.1
prunus_persica Prupe.4G006100_v2.0.a1
pyrus_communis pycom05g32650 pycom10g28650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 888
AclI AACGTT 1 cut(s) 551
AclWI GGATC 3 cut(s) 98, 117, 251
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 3 cut(s) 134, 178, 757
AcuI CTGAAG 2 cut(s) 543, 684
AfaI GTAC 6 cut(s) 152, 249, 332, 600, 782, 799
AfiI CCNNNNNNNGG 1 cut(s) 324
AgsI TTSAA 7 cut(s) 184, 265, 274, 453, 677, 811, 863
AjnI CCWGG 1 cut(s) 504
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 573, 605
AluBI AGCT 5 cut(s) 47, 125, 424, 668, 748
AluI AGCT 5 cut(s) 47, 125, 424, 668, 748
Alw26I GTCTC 2 cut(s) 199, 501
AlwI GGATC 3 cut(s) 98, 117, 251
AoxI GGCC 2 cut(s) 21, 502
ApeKI GCWGC 1 cut(s) 658
ApoI RAATTY 3 cut(s) 134, 178, 757
ArsI GACNNNNNNTTYG 2 cut(s) 163, 195
Asp700I GAANNNNTTC 1 cut(s) 448
AspLEI GCGC 1 cut(s) 120
AspS9I GGNCC 1 cut(s) 21
AsuNHI GCTAGC 1 cut(s) 748
BbsI GAAGAC 1 cut(s) 441
BbvI GCAGC 1 cut(s) 670
BccI CCATC 2 cut(s) 503, 887
BceAI ACGGC 1 cut(s) 799
BcgI CGANNNNNNTGC 2 cut(s) 360, 394
BciT130I CCWGG 1 cut(s) 506
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 2 cut(s) 199, 501
BfaI CTAG 3 cut(s) 44, 608, 749
BfoI RGCGCY 1 cut(s) 121
BglII AGATCT 1 cut(s) 604
BisI GCNGC 2 cut(s) 659, 888
BlsI GCNGC 2 cut(s) 660, 889
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 1 cut(s) 506
BmgT120I GGNCC 1 cut(s) 21
BmrFI CCNGG 1 cut(s) 506
BmtI GCTAGC 1 cut(s) 752
BpiI GAAGAC 1 cut(s) 441
BpuEI CTTGAG 1 cut(s) 341
Bsa29I ATCGAT 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 436
Bsc4I CCNNNNNNNGG 1 cut(s) 324
Bse3DI GCAATG 3 cut(s) 290, 412, 882
BseBI CCWGG 1 cut(s) 506
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 1 cut(s) 514
BseLI CCNNNNNNNGG 1 cut(s) 324
BseMI GCAATG 3 cut(s) 290, 412, 882
BseMII CTCAG 2 cut(s) 210, 474
BseXI GCAGC 1 cut(s) 670
BshFI GGCC 2 cut(s) 23, 504
BshVI ATCGAT 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 324
BsmAI GTCTC 2 cut(s) 199, 501
BsmBI CGTCTC 1 cut(s) 501
BsnI GGCC 2 cut(s) 23, 504
Bsp143I GATC 6 cut(s) 90, 94, 109, 256, 604, 775
BspACI CCGC 1 cut(s) 888
BspANI GGCC 2 cut(s) 23, 504
BspCNI CTCAG 2 cut(s) 209, 475
BspDI ATCGAT 1 cut(s) 93
BspOI GCTAGC 1 cut(s) 752
BspPI GGATC 3 cut(s) 98, 117, 251
BsrDI GCAATG 3 cut(s) 290, 412, 882
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 6 cut(s) 90, 94, 109, 256, 604, 775
BssT1I CCWWGG 1 cut(s) 436
Bst2UI CCWGG 1 cut(s) 506
Bst6I CTCTTC 1 cut(s) 819
BstC8I GCNNGC 1 cut(s) 750
BstDEI CTNAG 2 cut(s) 196, 483
BstF5I GGATG 1 cut(s) 514
BstH2I RGCGCY 1 cut(s) 121
BstHHI GCGC 1 cut(s) 120
BstKTI GATC 6 cut(s) 93, 97, 112, 259, 607, 778
BstMAI GTCTC 2 cut(s) 199, 501
BstMBI GATC 6 cut(s) 90, 94, 109, 256, 604, 775
BstMWI GCNNNNNNNGC 3 cut(s) 368, 524, 792
BstNI CCWGG 1 cut(s) 506
BstSCI CCNGG 1 cut(s) 504
BstV1I GCAGC 1 cut(s) 670
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 2 cut(s) 23, 504
BsuTUI ATCGAT 1 cut(s) 93
BtsCI GGATG 1 cut(s) 514
BtsIMutI CAGTG 1 cut(s) 204
Cac8I GCNNGC 1 cut(s) 750
CfoI GCGC 1 cut(s) 120
Cfr13I GGNCC 1 cut(s) 21
ClaI ATCGAT 1 cut(s) 93
Csp6I GTAC 6 cut(s) 151, 248, 331, 599, 781, 798
CviAII CATG 1 cut(s) 647
CviQI GTAC 6 cut(s) 151, 248, 331, 599, 781, 798
DdeI CTNAG 2 cut(s) 196, 483
DpnI GATC 6 cut(s) 92, 96, 111, 258, 606, 777
DpnII GATC 6 cut(s) 90, 94, 109, 256, 604, 775
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 819
EarI CTCTTC 1 cut(s) 819
Eco130I CCWWGG 1 cut(s) 436
Eco57I CTGAAG 2 cut(s) 543, 684
EcoRII CCWGG 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 436
ErhI CCWWGG 1 cut(s) 436
Esp3I CGTCTC 1 cut(s) 501
FaeI CATG 1 cut(s) 650
FatI CATG 1 cut(s) 646
FauNDI CATATG 1 cut(s) 86
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 659, 888
FokI GGATG 1 cut(s) 521
Fsp4HI GCNGC 2 cut(s) 659, 888
FspBI CTAG 3 cut(s) 44, 608, 749
GlaI GCGC 1 cut(s) 119
GluI GCNGC 2 cut(s) 659, 888
HaeII RGCGCY 1 cut(s) 121
HaeIII GGCC 2 cut(s) 23, 504
HhaI GCGC 1 cut(s) 120
Hin1II CATG 1 cut(s) 650
Hin6I GCGC 1 cut(s) 118
HinP1I GCGC 1 cut(s) 118
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HindIII AAGCTT 2 cut(s) 123, 666
HinfI GANTC 3 cut(s) 354, 413, 704
Hpy166II GTNNAC 2 cut(s) 248, 555
Hpy188I TCNGA 7 cut(s) 17, 114, 418, 433, 484, 562, 763
Hpy188III TCNNGA 3 cut(s) 254, 608, 856
Hpy8I GTNNAC 2 cut(s) 248, 555
HpyAV CCTTC 3 cut(s) 12, 271, 838
HpyCH4IV ACGT 2 cut(s) 551, 765
HpyCH4V TGCA 4 cut(s) 371, 399, 405, 658
HpyF10VI GCNNNNNNNGC 3 cut(s) 368, 524, 792
HpyF3I CTNAG 2 cut(s) 196, 483
HpySE526I ACGT 2 cut(s) 551, 765
Hsp92II CATG 1 cut(s) 650
HspAI GCGC 1 cut(s) 118
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 6 cut(s) 90, 94, 109, 256, 604, 775
LpnPI CCDG 5 cut(s) 222, 491, 518, 675, 841
Lsp1109I GCAGC 1 cut(s) 670
MaeI CTAG 3 cut(s) 44, 608, 749
MaeII ACGT 2 cut(s) 551, 765
MaeIII GTNAC 3 cut(s) 37, 51, 226
MalI GATC 6 cut(s) 92, 96, 111, 258, 606, 777
MboI GATC 6 cut(s) 90, 94, 109, 256, 604, 775
MflI RGATCY 1 cut(s) 604
MluCI AATT 6 cut(s) 134, 178, 385, 469, 757, 806
MlyI GAGTC 1 cut(s) 713
MmeI TCCRAC 3 cut(s) 456, 571, 748
MnlI CCTC 4 cut(s) 100, 168, 318, 841
MroXI GAANNNNTTC 1 cut(s) 448
MseI TTAA 3 cut(s) 468, 788, 892
MslI CAYNNNNRTG 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 506
MvaI CCWGG 1 cut(s) 506
MwoI GCNNNNNNNGC 3 cut(s) 368, 524, 792
NdeI CATATG 1 cut(s) 86
NdeII GATC 6 cut(s) 90, 94, 109, 256, 604, 775
NheI GCTAGC 1 cut(s) 748
NlaIII CATG 1 cut(s) 650
NmeAIII GCCGAG 1 cut(s) 480
NmuCI GTSAC 1 cut(s) 51
PdmI GAANNNNTTC 1 cut(s) 448
PfeI GAWTC 2 cut(s) 354, 413
PkrI GCNGC 2 cut(s) 660, 889
PleI GAGTC 1 cut(s) 712
PpsI GAGTC 1 cut(s) 712
Psp1406I AACGTT 1 cut(s) 551
Psp6I CCWGG 1 cut(s) 504
PspGI CCWGG 1 cut(s) 504
PspPI GGNCC 1 cut(s) 21
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 6 cut(s) 152, 249, 332, 600, 782, 799
RsaNI GTAC 6 cut(s) 151, 248, 331, 599, 781, 798
RseI CAYNNNNRTG 1 cut(s) 85
SaqAI TTAA 3 cut(s) 468, 788, 892
SatI GCNGC 2 cut(s) 659, 888
Sau3AI GATC 6 cut(s) 90, 94, 109, 256, 604, 775
Sau96I GGNCC 1 cut(s) 21
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 713
ScrFI CCNGG 1 cut(s) 506
SetI ASST 9 cut(s) 49, 127, 241, 426, 438, 554, 670, 750, 768
SmiMI CAYNNNNRTG 1 cut(s) 85
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
Sse9I AATT 6 cut(s) 134, 178, 385, 469, 757, 806
SsiI CCGC 1 cut(s) 888
SspI AATATT 1 cut(s) 63
SspMI CTAG 3 cut(s) 44, 608, 749
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 1 cut(s) 436
TaiI ACGT 2 cut(s) 554, 768
TaqI TCGA 4 cut(s) 93, 141, 380, 822
TasI AATT 6 cut(s) 134, 178, 385, 469, 757, 806
TatI WGTACW 5 cut(s) 150, 247, 330, 598, 797
TauI GCSGC 1 cut(s) 890
TfiI GAWTC 2 cut(s) 354, 413
Tru1I TTAA 3 cut(s) 468, 788, 892
Tru9I TTAA 3 cut(s) 468, 788, 892
TscAI CASTG 1 cut(s) 204
TseFI GTSAC 1 cut(s) 51
TseI GCWGC 1 cut(s) 658
Tsp45I GTSAC 1 cut(s) 51
TspDTI ATGAA 8 cut(s) 17, 127, 426, 663, 728, 819, 820, 857
TspGWI ACGGA 1 cut(s) 749
TspRI CASTG 1 cut(s) 204
XapI RAATTY 3 cut(s) 134, 178, 757
XbaI TCTAGA 1 cut(s) 607
XmnI GAANNNNTTC 1 cut(s) 448
XspI CTAG 3 cut(s) 44, 608, 749
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.