pycom111g02260

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0024435
Physical Location & Seq
Reverse (-)
1355573 .. 1356845
1273 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 732 bp
ATGGGTACCAGCAACTCCGCCCATCGAGCCGGCGTTCAAAGGGCTGCAATGGGTATTAACAACTTTGCCCATCGAGCCGCTGCCTCCTCTTCGTCTCCCTCGAGACGCTCAAACAGCTGGAAGTACGAGGTGTTCCTGAGCTTTAGAGGCGACGACACACGCAATACCTTCACAGACCACCTCTCCATTGCGTTACGCAATGCCGGAATCAACACGTTTATAGACTACCAGCTCAGAATGGGAAAAAATATACAGAGTGAACTTTACCGAGAAATCGAAGAGTCGAGGATCGACGTCGTCATCTTCTCGAAGAGGTACGCGGAGTCCCGATGGTGCCTGAGGGAGCTGTCGAAGATCATGAGGTGCCGAGAAGTTCAAAAGGGGAAAATAGTCTATCCAATATTCTACAACGTTGACCCTTCTCAAGTCAGGAAACAGAATGGTAGTTTTTCGAAAGCATTTCAGAAGCACAAAAGGAATGAAGATCCAAATGAAGTCAAGCAGTGGAGAAAGGATCTTAAGGCTTCTGCAGATTTGAGTGGCCGGAATCTCAAAACCACTGCAGACGGACGTGAAGGATTGTTTATCGAGAAAATTATTGGAGACATCAAGGGACTACTGAAAATTACAAACTCAAAAGAAGCCAAGCGCTCAATTGGAATAGCTTTTCAAGTGCAAGACTTCAATACTCAACACTTAGATGTTGGAGTTAGATGTTGGAGGTTCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

244

Amino Acids

27.93

Weight (kDa)

10.03

Isoelectric Point (pI)

42.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 41 - 212 4.5e-50 TIR domain
TIR_2 PF13676 44 - 141 4.1e-17 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0024435)

Species Orthologous Gene IDs
malus_domestica MD17G1023900.v1.1 MD17G1024100.v1.1
pyrus_communis pycom111g02260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
Acc65I GGTACC 1 cut(s) 5
AccB1I GGYRCC 3 cut(s) 5, 333, 363
AccII CGCG 1 cut(s) 320
AciI CCGC 3 cut(s) 18, 78, 320
AclI AACGTT 1 cut(s) 411
AclWI GGATC 3 cut(s) 296, 479, 522
AcoI YGGCCR 1 cut(s) 541
AcyI GRCGYC 1 cut(s) 294
AfaI GTAC 3 cut(s) 7, 125, 317
AfeI AGCGCT 1 cut(s) 650
AflII CTTAAG 1 cut(s) 518
AflIII ACRYGT 1 cut(s) 213
AgsI TTSAA 4 cut(s) 38, 377, 671, 685
AjiI CACGTC 1 cut(s) 572
AluBI AGCT 5 cut(s) 117, 141, 232, 346, 665
AluI AGCT 5 cut(s) 117, 141, 232, 346, 665
Alw26I GTCTC 3 cut(s) 97, 99, 597
AlwI GGATC 3 cut(s) 296, 479, 522
Ama87I CYCGRG 1 cut(s) 100
Aor51HI AGCGCT 1 cut(s) 650
AoxI GGCC 1 cut(s) 541
ApeKI GCWGC 2 cut(s) 44, 80
Asp718I GGTACC 1 cut(s) 5
AspLEI GCGC 1 cut(s) 651
AsuII TTCGAA 1 cut(s) 452
AvaI CYCGRG 1 cut(s) 100
AxyI CCTNAGG 1 cut(s) 338
BanI GGYRCC 3 cut(s) 5, 333, 363
BbvI GCAGC 2 cut(s) 31, 67
BccI CCATC 3 cut(s) 30, 78, 324
BcoDI GTCTC 3 cut(s) 97, 99, 597
BfmI CTRYAG 2 cut(s) 528, 561
BfoI RGCGCY 1 cut(s) 652
BfrI CTTAAG 1 cut(s) 518
BisI GCNGC 3 cut(s) 45, 78, 81
BlsI GCNGC 3 cut(s) 46, 79, 82
BmeT110I CYCGRG 1 cut(s) 100
BmgBI CACGTC 1 cut(s) 572
BmiI GGNNCC 3 cut(s) 7, 335, 365
Bpu10I CCTNAGC 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 452
BpuEI CTTGAG 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 294
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bse118I RCCGGY 1 cut(s) 29
Bse21I CCTNAGG 1 cut(s) 338
Bse3DI GCAATG 3 cut(s) 54, 186, 205
BseMI GCAATG 3 cut(s) 54, 186, 205
BseMII CTCAG 3 cut(s) 128, 247, 329
BseRI GAGGAG 1 cut(s) 76
BseXI GCAGC 2 cut(s) 31, 67
Bsh1236I CGCG 1 cut(s) 320
BshFI GGCC 1 cut(s) 543
BshNI GGYRCC 3 cut(s) 5, 333, 363
BsiHKCI CYCGRG 1 cut(s) 100
BsiSI CCGG 3 cut(s) 30, 204, 544
BslFI GGGAC 2 cut(s) 310, 627
BsmAI GTCTC 3 cut(s) 97, 99, 597
BsmBI CGTCTC 2 cut(s) 97, 99
BsmFI GGGAC 2 cut(s) 310, 627
BsnI GGCC 1 cut(s) 543
BsoBI CYCGRG 1 cut(s) 100
Bsp119I TTCGAA 1 cut(s) 452
Bsp143I GATC 4 cut(s) 288, 354, 484, 514
BspACI CCGC 3 cut(s) 18, 78, 320
BspANI GGCC 1 cut(s) 543
BspCNI CTCAG 3 cut(s) 129, 246, 330
BspFNI CGCG 1 cut(s) 320
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 3 cut(s) 7, 335, 365
BspMAI CTGCAG 2 cut(s) 532, 565
BspPI GGATC 3 cut(s) 296, 479, 522
BspT104I TTCGAA 1 cut(s) 452
BspT107I GGYRCC 3 cut(s) 5, 333, 363
BspTI CTTAAG 1 cut(s) 518
BsrDI GCAATG 3 cut(s) 54, 186, 205
BsrFI RCCGGY 1 cut(s) 29
BssAI RCCGGY 1 cut(s) 29
BssMI GATC 4 cut(s) 288, 354, 484, 514
BssNI GRCGYC 1 cut(s) 294
Bst6I CTCTTC 3 cut(s) 94, 273, 305
BstACI GRCGYC 1 cut(s) 294
BstAFI CTTAAG 1 cut(s) 518
BstBI TTCGAA 1 cut(s) 452
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 4 cut(s) 137, 233, 338, 697
BstFNI CGCG 1 cut(s) 320
BstH2I RGCGCY 1 cut(s) 652
BstHHI GCGC 1 cut(s) 651
BstKTI GATC 4 cut(s) 291, 357, 487, 517
BstMAI GTCTC 3 cut(s) 97, 99, 597
BstMBI GATC 4 cut(s) 288, 354, 484, 514
BstMWI GCNNNNNNNGC 4 cut(s) 26, 74, 114, 147
BstSFI CTRYAG 2 cut(s) 528, 561
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 31, 67
BstX2I RGATCY 2 cut(s) 484, 514
BstYI RGATCY 2 cut(s) 484, 514
Bsu36I CCTNAGG 1 cut(s) 338
BsuRI GGCC 1 cut(s) 543
BtrI CACGTC 1 cut(s) 572
BtsI GCAGTG 2 cut(s) 509, 558
BtsIMutI CAGTG 2 cut(s) 509, 558
Cac8I GCNNGC 1 cut(s) 31
CciI TCATGA 1 cut(s) 357
CfoI GCGC 1 cut(s) 651
Cfr10I RCCGGY 1 cut(s) 29
CseI GACGC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 6, 124, 316
CviAII CATG 1 cut(s) 358
CviQI GTAC 3 cut(s) 6, 124, 316
DdeI CTNAG 4 cut(s) 137, 233, 338, 697
DpnI GATC 4 cut(s) 290, 356, 486, 516
DpnII GATC 4 cut(s) 288, 354, 484, 514
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 3 cut(s) 94, 273, 305
EarI CTCTTC 3 cut(s) 94, 273, 305
EciI GGCGGA 1 cut(s) 7
Eco47III AGCGCT 1 cut(s) 650
Eco81I CCTNAGG 1 cut(s) 338
Eco88I CYCGRG 1 cut(s) 100
Esp3I CGTCTC 2 cut(s) 97, 99
FaeI CATG 1 cut(s) 361
FaiI YATR 3 cut(s) 221, 251, 359
FaqI GGGAC 2 cut(s) 310, 627
FatI CATG 1 cut(s) 357
Fnu4HI GCNGC 3 cut(s) 45, 78, 81
Fsp4HI GCNGC 3 cut(s) 45, 78, 81
GlaI GCGC 1 cut(s) 650
GluI GCNGC 3 cut(s) 45, 78, 81
HaeII RGCGCY 1 cut(s) 652
HaeIII GGCC 1 cut(s) 543
HapII CCGG 3 cut(s) 30, 204, 544
HgaI GACGC 1 cut(s) 114
HhaI GCGC 1 cut(s) 651
Hin1I GRCGYC 1 cut(s) 294
Hin1II CATG 1 cut(s) 361
Hin6I GCGC 1 cut(s) 649
HinP1I GCGC 1 cut(s) 649
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HinfI GANTC 4 cut(s) 207, 281, 323, 547
HpaII CCGG 3 cut(s) 30, 204, 544
Hpy166II GTNNAC 3 cut(s) 260, 415, 726
Hpy188I TCNGA 2 cut(s) 236, 465
Hpy188III TCNNGA 7 cut(s) 102, 136, 307, 327, 358, 430, 589
Hpy8I GTNNAC 3 cut(s) 260, 415, 726
Hpy99I CGWCG 3 cut(s) 155, 296, 299
HpyAV CCTTC 3 cut(s) 178, 429, 569
HpyCH4IV ACGT 4 cut(s) 215, 294, 411, 571
HpyCH4V TGCA 4 cut(s) 47, 530, 563, 676
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 74, 114, 147
HpyF3I CTNAG 4 cut(s) 137, 233, 338, 697
HpySE526I ACGT 4 cut(s) 215, 294, 411, 571
Hsp92I GRCGYC 1 cut(s) 294
Hsp92II CATG 1 cut(s) 361
HspAI GCGC 1 cut(s) 649
KpnI GGTACC 1 cut(s) 9
KroI GCCGGC 1 cut(s) 29
KroNI GCCGGC 1 cut(s) 31
Kzo9I GATC 4 cut(s) 288, 354, 484, 514
LmnI GCTCC 1 cut(s) 343
LpnPI CCDG 9 cut(s) 22, 43, 103, 149, 217, 242, 350, 415, 557
Lsp1109I GCAGC 2 cut(s) 31, 67
MaeII ACGT 4 cut(s) 215, 294, 411, 571
MaeIII GTNAC 1 cut(s) 192
MalI GATC 4 cut(s) 290, 356, 486, 516
MboI GATC 4 cut(s) 288, 354, 484, 514
MboII GAAGA 6 cut(s) 81, 290, 295, 322, 364, 494
MfeI CAATTG 1 cut(s) 654
MflI RGATCY 2 cut(s) 484, 514
MluCI AATT 3 cut(s) 594, 624, 654
MlyI GAGTC 2 cut(s) 290, 332
MmeI TCCRAC 2 cut(s) 685, 698
MroNI GCCGGC 1 cut(s) 29
MseI TTAA 3 cut(s) 57, 519, 730
MslI CAYNNNNRTG 1 cut(s) 699
MspA1I CMGCKG 2 cut(s) 80, 117
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 3 cut(s) 30, 204, 544
MunI CAATTG 1 cut(s) 654
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 4 cut(s) 26, 74, 114, 147
NaeI GCCGGC 1 cut(s) 31
NdeII GATC 4 cut(s) 288, 354, 484, 514
NgoMIV GCCGGC 1 cut(s) 29
NlaIII CATG 1 cut(s) 361
NlaIV GGNNCC 3 cut(s) 7, 335, 365
NmeAIII GCCGAG 1 cut(s) 392
NspV TTCGAA 1 cut(s) 452
PaeR7I CTCGAG 1 cut(s) 100
PagI TCATGA 1 cut(s) 357
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PdiI GCCGGC 1 cut(s) 31
PfeI GAWTC 2 cut(s) 207, 547
PflFI GACNNNGTC 1 cut(s) 296
PkrI GCNGC 3 cut(s) 46, 79, 82
PleI GAGTC 2 cut(s) 289, 331
PpsI GAGTC 2 cut(s) 289, 331
Psp1406I AACGTT 1 cut(s) 411
PspN4I GGNNCC 3 cut(s) 7, 335, 365
PsrI GAACNNNNNNTAC 2 cut(s) 116, 148
PstI CTGCAG 2 cut(s) 532, 565
PsuI RGATCY 2 cut(s) 484, 514
PsyI GACNNNGTC 1 cut(s) 296
PvuII CAGCTG 1 cut(s) 117
RsaI GTAC 3 cut(s) 7, 125, 317
RsaNI GTAC 3 cut(s) 6, 124, 316
RseI CAYNNNNRTG 1 cut(s) 699
SaqAI TTAA 3 cut(s) 57, 519, 730
SatI GCNGC 3 cut(s) 45, 78, 81
Sau3AI GATC 4 cut(s) 288, 354, 484, 514
SchI GAGTC 2 cut(s) 290, 332
SfcI CTRYAG 2 cut(s) 528, 561
Sfr274I CTCGAG 1 cut(s) 100
SfuI TTCGAA 1 cut(s) 452
SlaI CTCGAG 1 cut(s) 100
SmiMI CAYNNNNRTG 1 cut(s) 699
SmlI CTYRAG 3 cut(s) 100, 423, 518
SmoI CTYRAG 3 cut(s) 100, 423, 518
Sse9I AATT 3 cut(s) 594, 624, 654
SsiI CCGC 3 cut(s) 18, 78, 320
SspI AATATT 1 cut(s) 402
TaiI ACGT 4 cut(s) 218, 297, 414, 574
TasI AATT 3 cut(s) 594, 624, 654
TauI GCSGC 1 cut(s) 80
TfiI GAWTC 2 cut(s) 207, 547
Tru1I TTAA 3 cut(s) 57, 519, 730
Tru9I TTAA 3 cut(s) 57, 519, 730
TscAI CASTG 2 cut(s) 509, 565
TseI GCWGC 2 cut(s) 44, 80
TspDTI ATGAA 2 cut(s) 495, 507
TspGWI ACGGA 1 cut(s) 582
TspRI CASTG 2 cut(s) 509, 565
Tth111I GACNNNGTC 1 cut(s) 296
Vha464I CTTAAG 1 cut(s) 518
XhoI CTCGAG 1 cut(s) 100
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.