pycom11g06740

Josephin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0015189
Physical Location & Seq
Reverse (-)
5173555 .. 5174351
797 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 552 bp
ATGGCGACCGACAGCAGCTCCCAAATCTACCACGAGCGGCAGAAGCTACAGTTTTGCCTCTTACATTCGCTCAACAATCTTTTCCAGAAAAAAGATGCATTTACTCGATCAAGCCTGAATGAGGTTGCTGAAAAACTTGTGCTTGAAGAACCCAACAAGGAAAAATGGACACCCTTTTCTGTGCTCTTTAAACCCCATCATAATTCACTCACCGGAAACTATGACATAAATGTGCTAATTGCTGCTTTAGAAGACAAGGGGAAGAATGTGGTTTGGCACGATCGGCGTAATGGGGCGTCTACGATTGATCTAGACGGGCCTGAAGATGCTAATTTGATGGGTGTTCTGCTTAATGTTCCGGTTAGAATGTTTGCCGGGTTATGGAAAAGTAGGCATTGGGTAACGTTGAGGAAGATTGGTGGGGTTTGGTACAATTTGGATAGTGATCTTGTTGCTCCTGAGGCCTTTGAAGATGCTGAAAAGGTTAGAGAATTCTTGGATTACATGATTGGACATGGTGGAGAAGTTTTGCTTGTTATGAATAATAAATAG

Protein Analysis

184

Amino Acids

20.91

Weight (kDa)

5.97

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Josephin PF02099 14 - 179 4.5e-37 Josephin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015189)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G29640
fragaria_vesca FvH4_3g38250 FvH4_3g38250
malus_domestica MD11G1079900.v1.1
prunus_persica Prupe.6G060500_v2.0.a1
pyrus_communis pycom11g06740
rosa_chinensis RchiOBHm_Chr5g0068731
rosa_laevigata RLG00000036006
rosa_multiflora Rmu_sc0000376.1_g000026
rosa_roxburghii Rroxscaffold_1G00012090
rosa_rugosa Rorug05G0392700
rosa_samantha Rh5AG450200 Rh5BG468600 Rh5CG490800 Rh5DG482100
rosa_wichuraiana Rw5G042040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 37
AccI GTMKAC 1 cut(s) 299
AciI CCGC 1 cut(s) 37
AclI AACGTT 1 cut(s) 404
AcsI RAATTY 1 cut(s) 491
AcuI CTGAAG 1 cut(s) 342
AcyI GRCGYC 1 cut(s) 296
AfaI GTAC 1 cut(s) 431
AfiI CCNNNNNNNGG 2 cut(s) 121, 381
AgsI TTSAA 2 cut(s) 146, 470
AluBI AGCT 2 cut(s) 18, 46
AluI AGCT 2 cut(s) 18, 46
Alw21I GWGCWC 1 cut(s) 186
AoxI GGCC 2 cut(s) 317, 462
ApeKI GCWGC 2 cut(s) 15, 242
ApoI RAATTY 1 cut(s) 491
AspS9I GGNCC 1 cut(s) 317
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 202
AxyI CCTNAGG 1 cut(s) 459
BauI CACGAG 1 cut(s) 32
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 27, 229
BccI CCATC 2 cut(s) 204, 331
BcnI CCSGG 1 cut(s) 376
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 1 cut(s) 47
BisI GCNGC 3 cut(s) 16, 38, 243
BlsI GCNGC 3 cut(s) 17, 39, 244
Bme1390I CCNGG 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 317
BmrFI CCNGG 1 cut(s) 376
BmsI GCATC 3 cut(s) 85, 316, 463
BpiI GAAGAC 1 cut(s) 258
BpuMI CCSGG 1 cut(s) 376
BsaBI GATNNNNATC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 296
BsaWI WCCGGW 2 cut(s) 212, 358
BsaXI ACNNNNNCTCC 1 cut(s) 32
Bsc4I CCNNNNNNNGG 2 cut(s) 121, 381
Bse21I CCTNAGG 1 cut(s) 459
Bse8I GATNNNNATC 1 cut(s) 444
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 2 cut(s) 121, 381
BseMII CTCAG 1 cut(s) 450
BseXI GCAGC 2 cut(s) 27, 229
Bsh1285I CGRYCG 2 cut(s) 9, 283
BshFI GGCC 2 cut(s) 319, 464
BsiEI CGRYCG 2 cut(s) 9, 283
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 3 cut(s) 213, 359, 375
BslI CCNNNNNNNGG 2 cut(s) 121, 381
BsnI GGCC 2 cut(s) 319, 464
Bsp1286I GDGCHC 1 cut(s) 186
Bsp143I GATC 4 cut(s) 107, 280, 307, 445
BspACI CCGC 1 cut(s) 37
BspANI GGCC 2 cut(s) 319, 464
BspCNI CTCAG 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 37
BssMI GATC 4 cut(s) 107, 280, 307, 445
BssNI GRCGYC 1 cut(s) 296
BssSI CACGAG 1 cut(s) 32
Bst2BI CACGAG 1 cut(s) 32
Bst4CI ACNGT 1 cut(s) 51
BstACI GRCGYC 1 cut(s) 296
BstDEI CTNAG 1 cut(s) 459
BstENI CCTNNNNNAGG 1 cut(s) 119
BstKTI GATC 4 cut(s) 110, 283, 310, 448
BstMBI GATC 4 cut(s) 107, 280, 307, 445
BstMCI CGRYCG 2 cut(s) 9, 283
BstMWI GCNNNNNNNGC 3 cut(s) 43, 283, 461
BstSCI CCNGG 1 cut(s) 374
BstSFI CTRYAG 1 cut(s) 47
BstV1I GCAGC 2 cut(s) 27, 229
BstV2I GAAGAC 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 459
BsuRI GGCC 2 cut(s) 319, 464
Cfr13I GGNCC 1 cut(s) 317
CseI GACGC 1 cut(s) 285
Csp6I GTAC 1 cut(s) 430
CviAII CATG 2 cut(s) 505, 515
CviJI RGCY 5 cut(s) 18, 46, 114, 319, 464
CviKI_1 RGCY 5 cut(s) 18, 46, 114, 319, 464
CviQI GTAC 1 cut(s) 430
DdeI CTNAG 1 cut(s) 459
DpnI GATC 4 cut(s) 109, 282, 309, 447
DpnII GATC 4 cut(s) 107, 280, 307, 445
DraI TTTAAA 1 cut(s) 190
Eco147I AGGCCT 1 cut(s) 464
Eco57I CTGAAG 1 cut(s) 342
Eco81I CCTNAGG 1 cut(s) 459
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 491
EcoT22I ATGCAT 1 cut(s) 100
FaeI CATG 2 cut(s) 508, 518
FaiI YATR 7 cut(s) 201, 222, 227, 382, 506, 516, 539
FalI AAGNNNNNCTT 2 cut(s) 516, 548
FatI CATG 2 cut(s) 504, 514
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 3 cut(s) 16, 38, 243
Fsp4HI GCNGC 3 cut(s) 16, 38, 243
FspBI CTAG 1 cut(s) 311
GluI GCNGC 3 cut(s) 16, 38, 243
HaeIII GGCC 2 cut(s) 319, 464
HapII CCGG 3 cut(s) 213, 359, 375
HgaI GACGC 1 cut(s) 285
Hin1I GRCGYC 1 cut(s) 296
Hin1II CATG 2 cut(s) 508, 518
HpaII CCGG 3 cut(s) 213, 359, 375
HphI GGTGA 1 cut(s) 202
Hpy166II GTNNAC 1 cut(s) 300
Hpy188III TCNNGA 3 cut(s) 85, 311, 458
Hpy8I GTNNAC 1 cut(s) 300
HpyCH4III ACNGT 1 cut(s) 51
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 1 cut(s) 98
HpyF10VI GCNNNNNNNGC 3 cut(s) 43, 283, 461
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 1 cut(s) 404
Hsp92I GRCGYC 1 cut(s) 296
Hsp92II CATG 2 cut(s) 508, 518
Kzo9I GATC 4 cut(s) 107, 280, 307, 445
LmnI GCTCC 2 cut(s) 23, 460
LpnPI CCDG 7 cut(s) 98, 128, 226, 333, 372, 388, 471
Lsp1109I GCAGC 2 cut(s) 27, 229
LweI GCATC 3 cut(s) 85, 316, 463
MaeI CTAG 1 cut(s) 311
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 1 cut(s) 400
MalI GATC 4 cut(s) 109, 282, 309, 447
MbiI CCGCTC 1 cut(s) 37
MboI GATC 4 cut(s) 107, 280, 307, 445
MboII GAAGA 6 cut(s) 158, 263, 274, 335, 424, 482
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 5 cut(s) 202, 237, 331, 433, 491
MnlI CCTC 4 cut(s) 68, 115, 402, 454
Mph1103I ATGCAT 1 cut(s) 100
MseI TTAA 2 cut(s) 189, 351
MslI CAYNNNNRTG 1 cut(s) 230
MspI CCGG 3 cut(s) 213, 359, 375
MspR9I CCNGG 1 cut(s) 376
MwoI GCNNNNNNNGC 3 cut(s) 43, 283, 461
NciI CCSGG 1 cut(s) 376
NdeII GATC 4 cut(s) 107, 280, 307, 445
NlaIII CATG 2 cut(s) 508, 518
NsiI ATGCAT 1 cut(s) 100
PceI AGGCCT 1 cut(s) 464
PkrI GCNGC 3 cut(s) 17, 39, 244
Ple19I CGATCG 1 cut(s) 283
Psp1406I AACGTT 1 cut(s) 404
PspPI GGNCC 1 cut(s) 317
PvuI CGATCG 1 cut(s) 283
RsaI GTAC 1 cut(s) 431
RsaNI GTAC 1 cut(s) 430
RseI CAYNNNNRTG 1 cut(s) 230
SaqAI TTAA 2 cut(s) 189, 351
SatI GCNGC 3 cut(s) 16, 38, 243
Sau3AI GATC 4 cut(s) 107, 280, 307, 445
Sau96I GGNCC 1 cut(s) 317
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 1 cut(s) 186
SetI ASST 5 cut(s) 20, 48, 126, 407, 486
SfaNI GCATC 3 cut(s) 85, 316, 463
SfcI CTRYAG 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 230
Sse9I AATT 5 cut(s) 202, 237, 331, 433, 491
SseBI AGGCCT 1 cut(s) 464
SsiI CCGC 1 cut(s) 37
SspMI CTAG 1 cut(s) 311
StuI AGGCCT 1 cut(s) 464
StyD4I CCNGG 1 cut(s) 374
TaaI ACNGT 1 cut(s) 51
TaiI ACGT 1 cut(s) 407
TaqI TCGA 1 cut(s) 106
TaqII GACCGA 1 cut(s) 23
TasI AATT 5 cut(s) 202, 237, 331, 433, 491
TauI GCSGC 1 cut(s) 40
Tru1I TTAA 2 cut(s) 189, 351
Tru9I TTAA 2 cut(s) 189, 351
TseI GCWGC 2 cut(s) 15, 242
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 1 cut(s) 491
XbaI TCTAGA 1 cut(s) 310
XmiI GTMKAC 1 cut(s) 299
XspI CTAG 1 cut(s) 311
Zsp2I ATGCAT 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.