pycom11g10030

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0022552
Physical Location & Seq
Forward (+)
8392013 .. 8392665
653 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 354 bp
ATGCCCTACAAGGAGGTTCCAATCATCTTCGGAGAGTGGTGGAATGCAGATCCTGAGGCAGTTATTAGCCAGGCCTTGCAAACAGGTGGAGGACCAAATGTCTCCGATGCTTATACTTTCAATGGATTCCCAGGGCCCATGTACAACTGTTCTGCCAAAGATGCATTCAAGCTAAAGGTTCAATCTGGGAAGACCTACCTTCTCCGGCTAATCAATGCAGCACTCAATGACGAGCTCTTCTTCAGCATAGCGAACCACACCCTCAAAGTGGTAGAAGCCGACGCCATTTATGTCAAACTACAAATGTTCTGCTCAAGACCAAACCCCATTTCCCCAGTGCCACATTTTTTATGA

Protein Analysis

118

Amino Acids

13.0

Weight (kDa)

6.04

Isoelectric Point (pI)

32.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 5 - 99 2.2e-28 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022552)

Species Orthologous Gene IDs
pyrus_communis pycom11g10030
rosa_samantha Rh5BG001200 Rh5DG433300 Rh5DG434200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 44
AcuI CTGAAG 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 1 cut(s) 143
AfiI CCNNNNNNNGG 1 cut(s) 268
AgsI TTSAA 3 cut(s) 121, 169, 182
AhdI GACNNNNNGTC 1 cut(s) 98
AjnI CCWGG 2 cut(s) 69, 130
AjuI GAANNNNNNNTTGG 4 cut(s) 149, 181, 313, 345
AluBI AGCT 2 cut(s) 172, 235
AluI AGCT 2 cut(s) 172, 235
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 1 cut(s) 44
AlwNI CAGNNNCTG 1 cut(s) 53
AoxI GGCC 2 cut(s) 72, 134
ApaI GGGCCC 1 cut(s) 138
ApeKI GCWGC 1 cut(s) 218
AspS9I GGNCC 3 cut(s) 92, 134, 135
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 54
BaeGI GKGCMC 1 cut(s) 138
BanII GRGCYC 2 cut(s) 138, 237
BbsI GAAGAC 1 cut(s) 197
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 1 cut(s) 230
BciT130I CCWGG 2 cut(s) 71, 132
BcoDI GTCTC 1 cut(s) 106
BisI GCNGC 1 cut(s) 219
BlsI GCNGC 1 cut(s) 220
Bme1390I CCNGG 2 cut(s) 71, 132
Bme18I GGWCC 1 cut(s) 92
BmeRI GACNNNNNGTC 1 cut(s) 98
BmgT120I GGNCC 3 cut(s) 92, 134, 135
BmiI GGNNCC 2 cut(s) 18, 136
BmrFI CCNGG 2 cut(s) 71, 132
BmrI ACTGGG 1 cut(s) 329
BmsI GCATC 2 cut(s) 97, 151
BmuI ACTGGG 1 cut(s) 329
BpiI GAAGAC 1 cut(s) 197
BpuEI CTTGAG 1 cut(s) 298
BsaHI GRCGYC 1 cut(s) 282
BsaJI CCNNGG 2 cut(s) 130, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 268
Bse1I ACTGG 1 cut(s) 335
Bse21I CCTNAGG 1 cut(s) 54
BseBI CCWGG 2 cut(s) 71, 132
BseDI CCNNGG 2 cut(s) 130, 131
BseLI CCNNNNNNNGG 1 cut(s) 268
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 335
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 1 cut(s) 230
BshFI GGCC 2 cut(s) 74, 136
BsiHKAI GWGCWC 1 cut(s) 237
BsiSI CCGG 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 268
BsmAI GTCTC 1 cut(s) 106
BsmI GAATGC 2 cut(s) 49, 164
BsnI GGCC 2 cut(s) 74, 136
Bsp120I GGGCCC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 138, 237
Bsp1407I TGTACA 1 cut(s) 141
Bsp143I GATC 1 cut(s) 49
BspANI GGCC 2 cut(s) 74, 136
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 2 cut(s) 18, 136
BspPI GGATC 1 cut(s) 44
BspQI GCTCTTC 1 cut(s) 242
BsrGI TGTACA 1 cut(s) 141
BsrI ACTGG 1 cut(s) 335
BssECI CCNNGG 2 cut(s) 130, 131
BssMI GATC 1 cut(s) 49
BssNI GRCGYC 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 71, 132
Bst4CI ACNGT 1 cut(s) 149
Bst6I CTCTTC 1 cut(s) 242
BstACI GRCGYC 1 cut(s) 282
BstAUI TGTACA 1 cut(s) 141
BstDEI CTNAG 1 cut(s) 54
BstKTI GATC 1 cut(s) 52
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 1 cut(s) 49
BstMWI GCNNNNNNNGC 1 cut(s) 161
BstNI CCWGG 2 cut(s) 71, 132
BstSCI CCNGG 2 cut(s) 69, 130
BstSLI GKGCMC 1 cut(s) 138
BstV1I GCAGC 1 cut(s) 230
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 1 cut(s) 49
BstYI RGATCY 1 cut(s) 49
Bsu36I CCTNAGG 1 cut(s) 54
BsuRI GGCC 2 cut(s) 74, 136
BtsIMutI CAGTG 1 cut(s) 342
CaiI CAGNNNCTG 1 cut(s) 53
Cfr13I GGNCC 3 cut(s) 92, 134, 135
CseI GACGC 1 cut(s) 290
Csp6I GTAC 1 cut(s) 142
CviAII CATG 1 cut(s) 139
CviJI RGCY 7 cut(s) 69, 74, 136, 172, 208, 235, 278
CviKI_1 RGCY 7 cut(s) 69, 74, 136, 172, 208, 235, 278
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 1 cut(s) 54
DpnI GATC 1 cut(s) 51
DpnII GATC 1 cut(s) 49
DriI GACNNNNNGTC 1 cut(s) 98
Eam1104I CTCTTC 1 cut(s) 242
Eam1105I GACNNNNNGTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 242
Ecl136II GAGCTC 1 cut(s) 235
Eco147I AGGCCT 1 cut(s) 74
Eco24I GRGCYC 2 cut(s) 138, 237
Eco47I GGWCC 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 226
Eco81I CCTNAGG 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 134
EcoRII CCWGG 2 cut(s) 69, 130
EcoT22I ATGCAT 1 cut(s) 166
EcoT38I GRGCYC 2 cut(s) 138, 237
FaeI CATG 1 cut(s) 142
FaiI YATR 5 cut(s) 114, 140, 248, 291, 352
FatI CATG 1 cut(s) 138
Fnu4HI GCNGC 1 cut(s) 219
FriOI GRGCYC 2 cut(s) 138, 237
Fsp4HI GCNGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 219
HaeIII GGCC 2 cut(s) 74, 136
HapII CCGG 1 cut(s) 205
HgaI GACGC 1 cut(s) 290
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 1 cut(s) 142
HinfI GANTC 1 cut(s) 126
HpaII CCGG 1 cut(s) 205
Hpy188I TCNGA 2 cut(s) 32, 106
Hpy188III TCNNGA 2 cut(s) 53, 315
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 1 cut(s) 209
HpyCH4III ACNGT 1 cut(s) 149
HpyCH4V TGCA 4 cut(s) 47, 79, 164, 218
HpyF10VI GCNNNNNNNGC 1 cut(s) 161
HpyF3I CTNAG 1 cut(s) 54
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 1 cut(s) 142
Kzo9I GATC 1 cut(s) 49
LguI GCTCTTC 1 cut(s) 242
LpnPI CCDG 9 cut(s) 56, 66, 69, 83, 117, 144, 171, 218, 348
Lsp1109I GCAGC 1 cut(s) 230
LweI GCATC 2 cut(s) 97, 151
MalI GATC 1 cut(s) 51
MboI GATC 1 cut(s) 49
MboII GAAGA 4 cut(s) 19, 202, 229, 232
MflI RGATCY 1 cut(s) 49
MhlI GDGCHC 2 cut(s) 138, 237
MnlI CCTC 4 cut(s) 7, 49, 83, 272
Mph1103I ATGCAT 1 cut(s) 166
MspI CCGG 1 cut(s) 205
MspR9I CCNGG 2 cut(s) 71, 132
Mva1269I GAATGC 2 cut(s) 49, 164
MvaI CCWGG 2 cut(s) 71, 132
MwoI GCNNNNNNNGC 1 cut(s) 161
NdeII GATC 1 cut(s) 49
NlaIII CATG 1 cut(s) 142
NlaIV GGNNCC 2 cut(s) 18, 136
NsiI ATGCAT 1 cut(s) 166
PasI CCCWGGG 1 cut(s) 131
PceI AGGCCT 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 242
PctI GAATGC 2 cut(s) 49, 164
PfeI GAWTC 1 cut(s) 126
PkrI GCNGC 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 2 cut(s) 69, 130
PspGI CCWGG 2 cut(s) 69, 130
PspN4I GGNNCC 2 cut(s) 18, 136
PspOMI GGGCCC 1 cut(s) 134
PspPI GGNCC 3 cut(s) 92, 134, 135
PstNI CAGNNNCTG 1 cut(s) 53
PsuI RGATCY 1 cut(s) 49
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 242
SatI GCNGC 1 cut(s) 219
Sau3AI GATC 1 cut(s) 49
Sau96I GGNCC 3 cut(s) 92, 134, 135
ScrFI CCNGG 2 cut(s) 71, 132
SduI GDGCHC 2 cut(s) 138, 237
SetI ASST 7 cut(s) 18, 88, 174, 180, 197, 201, 237
SfaNI GCATC 2 cut(s) 97, 151
SinI GGWCC 1 cut(s) 92
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
SseBI AGGCCT 1 cut(s) 74
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 74
StyD4I CCNGG 2 cut(s) 69, 130
TaaI ACNGT 1 cut(s) 149
TatI WGTACW 1 cut(s) 141
TfiI GAWTC 1 cut(s) 126
TscAI CASTG 1 cut(s) 342
TseI GCWGC 1 cut(s) 218
TspRI CASTG 1 cut(s) 342
VpaK11BI GGWCC 1 cut(s) 92
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.