Rh5DG433300

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0022552
Physical Location & Seq
Forward (+)
69221049 .. 69229060
8012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG433300.1

Sequence Viewer

Length: 708 bp
ATGGAAGGCATGGATCGCCTCCGAAACGAGACGATGATCGTAAAGGGCATTATGTTTACAATCTCGGTTAGAATATCACTCCAAGATATAAAATACTTAACCAGATGGGTGAAGATGGTGCTAAGCATGGAAGTAGCTATAGCTTGGTCAACATCAGGAGATTGGTGGAATGTAGATCCTGAAGCAGTCATAAACAAGGCACTAAAAATTGGTGGAGGCCCAAATGTCTCAGACGCCTATACCATTAATGGACTGCCAGGACCATTGTATAACTGTTCGGCCAAAGATACTTTCAAGCTGAAGGTTCAAGCTGGGAAAACATACCTTCTTCGCCTAATCAATGCAACACTAAATGACGAGCTCTTCTTCAGCATTGCAAATCATACCCTCAAAGTGGTCGAAACAGACGCCGTTTATGTCAAGCCCTTCGACACCAACACCATTCTCATTGCCCTGGGACAAACCACAAACGTTCTTCTCAAAACCAAACCCAACTTCCCAAATGCTACATTTCTCATGACCGCAAGGCCATATGTGACTGGTCTAGGCACCTTCGACAACTCCACTCTTGCTGGCATTCTAGAGTTCGAAACGACCGCATTTCACTCAAACCTCCCACTGAACAAACTTCCACTCCACAAACCAACTCTGCCAGCTCTAAATGACACACGTTTGCAACAAAGTTCGCCAACAAACTCCGCAGCTTAA

Protein Analysis

235

Amino Acids

25.95

Weight (kDa)

8.91

Isoelectric Point (pI)

24.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase PF00394 51 - 195 7.9e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0022552)

Species Orthologous Gene IDs
pyrus_communis pycom11g10030
rosa_samantha Rh5BG001200 Rh5DG433300 Rh5DG434200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 548
AciI CCGC 3 cut(s) 522, 597, 699
AclI AACGTT 1 cut(s) 471
AclWI GGATC 2 cut(s) 21, 170
AcoI YGGCCR 1 cut(s) 279
AcuI CTGAAG 3 cut(s) 201, 320, 352
AcyI GRCGYC 2 cut(s) 234, 408
AfiI CCNNNNNNNGG 1 cut(s) 394
AflIII ACRYGT 1 cut(s) 668
AgsI TTSAA 2 cut(s) 295, 308
AjnI CCWGG 2 cut(s) 256, 453
AjuI GAANNNNNNNTTGG 4 cut(s) 275, 307, 479, 511
AluBI AGCT 7 cut(s) 137, 143, 298, 311, 361, 656, 704
AluI AGCT 7 cut(s) 137, 143, 298, 311, 361, 656, 704
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 23, 232
AlwI GGATC 2 cut(s) 21, 170
AoxI GGCC 3 cut(s) 217, 279, 527
ApeKI GCWGC 1 cut(s) 701
AseI ATTAAT 1 cut(s) 246
AspS9I GGNCC 2 cut(s) 218, 260
AsuHPI GGTGA 1 cut(s) 121
AsuII TTCGAA 1 cut(s) 588
AvaII GGWCC 1 cut(s) 260
BanI GGYRCC 1 cut(s) 548
BanII GRGCYC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 363
BccI CCATC 2 cut(s) 99, 109
BceAI ACGGC 1 cut(s) 395
BciT130I CCWGG 2 cut(s) 258, 455
BcoDI GTCTC 2 cut(s) 23, 232
BfaI CTAG 2 cut(s) 545, 581
BfmI CTRYAG 1 cut(s) 138
BisI GCNGC 1 cut(s) 702
BlpI GCTNAGC 1 cut(s) 122
BlsI GCNGC 1 cut(s) 703
Bme1390I CCNGG 2 cut(s) 258, 455
Bme18I GGWCC 1 cut(s) 260
BmgT120I GGNCC 2 cut(s) 218, 260
BmiI GGNNCC 1 cut(s) 550
BmrFI CCNGG 2 cut(s) 258, 455
Bpu1102I GCTNAGC 1 cut(s) 122
Bpu14I TTCGAA 1 cut(s) 588
BsaHI GRCGYC 2 cut(s) 234, 408
BsaJI CCNNGG 2 cut(s) 453, 454
BsaXI ACNNNNNCTCC 2 cut(s) 618, 648
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 2 cut(s) 372, 447
BseBI CCWGG 2 cut(s) 258, 455
BseDI CCNNGG 2 cut(s) 453, 454
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 2 cut(s) 372, 447
BseMII CTCAG 1 cut(s) 243
BseNI ACTGG 1 cut(s) 544
BseYI CCCAGC 1 cut(s) 311
Bsh1285I CGRYCG 1 cut(s) 597
BshFI GGCC 3 cut(s) 219, 281, 529
BshNI GGYRCC 1 cut(s) 548
BsiEI CGRYCG 1 cut(s) 597
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 1 cut(s) 471
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 2 cut(s) 23, 232
BsmBI CGTCTC 1 cut(s) 23
BsmFI GGGAC 1 cut(s) 471
BsmI GAATGC 1 cut(s) 576
BsnI GGCC 3 cut(s) 219, 281, 529
Bsp119I TTCGAA 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 3 cut(s) 13, 36, 175
Bsp1720I GCTNAGC 1 cut(s) 122
BspACI CCGC 3 cut(s) 522, 597, 699
BspANI GGCC 3 cut(s) 219, 281, 529
BspCNI CTCAG 1 cut(s) 242
BspHI TCATGA 1 cut(s) 516
BspLI GGNNCC 1 cut(s) 550
BspPI GGATC 2 cut(s) 21, 170
BspQI GCTCTTC 1 cut(s) 368
BspT104I TTCGAA 1 cut(s) 588
BspT107I GGYRCC 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 372, 447
BsrI ACTGG 1 cut(s) 544
BssECI CCNNGG 2 cut(s) 453, 454
BssMI GATC 3 cut(s) 13, 36, 175
BssNI GRCGYC 2 cut(s) 234, 408
Bst2UI CCWGG 2 cut(s) 258, 455
Bst4CI ACNGT 1 cut(s) 275
Bst6I CTCTTC 1 cut(s) 368
BstACI GRCGYC 2 cut(s) 234, 408
BstBI TTCGAA 1 cut(s) 588
BstC8I GCNNGC 2 cut(s) 574, 654
BstDEI CTNAG 2 cut(s) 122, 229
BstKTI GATC 3 cut(s) 16, 39, 178
BstMAI GTCTC 2 cut(s) 23, 232
BstMBI GATC 3 cut(s) 13, 36, 175
BstMCI CGRYCG 1 cut(s) 597
BstMWI GCNNNNNNNGC 1 cut(s) 15
BstNI CCWGG 2 cut(s) 258, 455
BstSCI CCNGG 2 cut(s) 256, 453
BstSFI CTRYAG 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 175
BstYI RGATCY 1 cut(s) 175
BsuRI GGCC 3 cut(s) 219, 281, 529
BtsIMutI CAGTG 1 cut(s) 617
Cac8I GCNNGC 2 cut(s) 574, 654
CciI TCATGA 1 cut(s) 516
Cfr13I GGNCC 2 cut(s) 218, 260
CseI GACGC 2 cut(s) 242, 416
CviAII CATG 3 cut(s) 10, 127, 517
DdeI CTNAG 2 cut(s) 122, 229
DpnI GATC 3 cut(s) 15, 38, 177
DpnII GATC 3 cut(s) 13, 36, 175
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Ecl136II GAGCTC 1 cut(s) 361
Eco24I GRGCYC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 361
Eco57I CTGAAG 3 cut(s) 201, 320, 352
EcoICRI GAGCTC 1 cut(s) 361
EcoRII CCWGG 2 cut(s) 256, 453
EcoT38I GRGCYC 1 cut(s) 363
Esp3I CGTCTC 1 cut(s) 23
FaeI CATG 3 cut(s) 13, 130, 520
FaqI GGGAC 1 cut(s) 471
FatI CATG 3 cut(s) 9, 126, 516
FauNDI CATATG 1 cut(s) 532
Fnu4HI GCNGC 1 cut(s) 702
FriOI GRGCYC 1 cut(s) 363
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 2 cut(s) 545, 581
GluI GCNGC 1 cut(s) 702
GsaI CCCAGC 1 cut(s) 315
HaeIII GGCC 3 cut(s) 219, 281, 529
HgaI GACGC 2 cut(s) 242, 416
Hin1I GRCGYC 2 cut(s) 234, 408
Hin1II CATG 3 cut(s) 13, 130, 520
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HphI GGTGA 1 cut(s) 121
Hpy166II GTNNAC 2 cut(s) 57, 150
Hpy188I TCNGA 2 cut(s) 23, 232
Hpy188III TCNNGA 4 cut(s) 156, 179, 517, 581
Hpy8I GTNNAC 2 cut(s) 57, 150
HpyAV CCTTC 4 cut(s) 295, 335, 436, 562
HpyCH4III ACNGT 1 cut(s) 275
HpyCH4IV ACGT 2 cut(s) 471, 670
HpyCH4V TGCA 3 cut(s) 344, 377, 676
HpyF10VI GCNNNNNNNGC 1 cut(s) 15
HpyF3I CTNAG 2 cut(s) 122, 229
HpySE526I ACGT 2 cut(s) 471, 670
Hsp92I GRCGYC 2 cut(s) 234, 408
Hsp92II CATG 3 cut(s) 13, 130, 520
Kzo9I GATC 3 cut(s) 13, 36, 175
LguI GCTCTTC 1 cut(s) 368
MaeI CTAG 2 cut(s) 545, 581
MaeII ACGT 2 cut(s) 471, 670
MaeIII GTNAC 1 cut(s) 535
MalI GATC 3 cut(s) 15, 38, 177
MboI GATC 3 cut(s) 13, 36, 175
MboII GAAGA 5 cut(s) 124, 320, 355, 358, 467
MflI RGATCY 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 1 cut(s) 207
MnlI CCTC 4 cut(s) 29, 209, 398, 623
MseI TTAA 3 cut(s) 98, 246, 706
MspR9I CCNGG 2 cut(s) 258, 455
Mva1269I GAATGC 1 cut(s) 576
MvaI CCWGG 2 cut(s) 258, 455
MwoI GCNNNNNNNGC 1 cut(s) 15
NdeI CATATG 1 cut(s) 532
NdeII GATC 3 cut(s) 13, 36, 175
NlaIII CATG 3 cut(s) 13, 130, 520
NlaIV GGNNCC 1 cut(s) 550
NmuCI GTSAC 1 cut(s) 535
NspV TTCGAA 1 cut(s) 588
PagI TCATGA 1 cut(s) 516
PasI CCCWGGG 1 cut(s) 454
PciSI GCTCTTC 1 cut(s) 368
PctI GAATGC 1 cut(s) 576
PkrI GCNGC 1 cut(s) 703
PshBI ATTAAT 1 cut(s) 246
Psp124BI GAGCTC 1 cut(s) 363
Psp1406I AACGTT 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 256, 453
PspFI CCCAGC 1 cut(s) 311
PspGI CCWGG 2 cut(s) 256, 453
PspN4I GGNNCC 1 cut(s) 550
PspPI GGNCC 2 cut(s) 218, 260
PsuI RGATCY 1 cut(s) 175
SacI GAGCTC 1 cut(s) 363
SapI GCTCTTC 1 cut(s) 368
SaqAI TTAA 3 cut(s) 98, 246, 706
SatI GCNGC 1 cut(s) 702
Sau3AI GATC 3 cut(s) 13, 36, 175
Sau96I GGNCC 2 cut(s) 218, 260
ScrFI CCNGG 2 cut(s) 258, 455
SduI GDGCHC 1 cut(s) 363
SfcI CTRYAG 1 cut(s) 138
SfuI TTCGAA 1 cut(s) 588
SinI GGWCC 1 cut(s) 260
Sse9I AATT 1 cut(s) 207
SsiI CCGC 3 cut(s) 522, 597, 699
SspMI CTAG 2 cut(s) 545, 581
SstI GAGCTC 1 cut(s) 363
StyD4I CCNGG 2 cut(s) 256, 453
TaaI ACNGT 1 cut(s) 275
TaiI ACGT 2 cut(s) 474, 673
TaqI TCGA 4 cut(s) 399, 429, 555, 588
TasI AATT 1 cut(s) 207
Tru1I TTAA 3 cut(s) 98, 246, 706
Tru9I TTAA 3 cut(s) 98, 246, 706
TscAI CASTG 1 cut(s) 624
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 535
TspRI CASTG 1 cut(s) 624
VpaK11BI GGWCC 1 cut(s) 260
VspI ATTAAT 1 cut(s) 246
XbaI TCTAGA 1 cut(s) 580
XspI CTAG 2 cut(s) 545, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.