pycom11g14450

Ubiquitin-conjugating enzyme E2 variant

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0021258
Physical Location & Seq
Forward (+)
14400692 .. 14402068
1377 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGTTATTTCTTAAATTATGGGCTTTAACTTTTACTTCACTTTTGCTGCCAGCCCGAGTCGTCAGTCGTCGTCCCCCTTTATACCCGCGTTCTCGTCCATCATTGTCTCTTCTGCTTTCTCTCAACAAATATTCCCTGCCCTCCCAAAACCCCTACCCATCTCTCAATTCCTTCCCCTCTTCCATGACGATTGGGTCTGCCGGATCCAGCATCATTGTGCCGCGAAATTTTAGGTTGCTGGAAGAGCTCGAGCGTGGAGAAAAAGGTATAGGAGATGGAACTGTAAGCTATGGAATGGATGATGGAGATGACATTTACATGCGCTCTTGGACGGGTACCATAATTGGTCCACTCAATGTAAGCAAGTGCCTCAATAATCCCCCCGCCTCCCTGTTTCTTCTCCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04624 map04624
Pfam Domains
Protein Families
UQ_con

Protein Analysis

136

Amino Acids

14.89

Weight (kDa)

9.51

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0021258)

Species Orthologous Gene IDs
pyrus_communis pycom03g10570 pycom11g14450
rosa_multiflora Rmu_co8203264.1_g000001
rosa_samantha Rh3BG227700 Rh3DG222900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 193
Acc65I GGTACC 1 cut(s) 335
AccB1I GGYRCC 1 cut(s) 335
AccII CGCG 2 cut(s) 88, 223
AciI CCGC 3 cut(s) 86, 221, 384
AclWI GGATC 2 cut(s) 198, 211
AcsI RAATTY 1 cut(s) 226
AfaI GTAC 1 cut(s) 337
AluBI AGCT 2 cut(s) 247, 288
AluI AGCT 2 cut(s) 247, 288
Alw21I GWGCWC 1 cut(s) 249
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 2 cut(s) 198, 211
Ama87I CYCGRG 2 cut(s) 54, 248
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 226
Asp718I GGTACC 1 cut(s) 335
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 1 cut(s) 347
AvaI CYCGRG 2 cut(s) 54, 248
AvaII GGWCC 1 cut(s) 347
BamHI GGATCC 1 cut(s) 203
BanI GGYRCC 1 cut(s) 335
BanII GRGCYC 1 cut(s) 249
Bbv12I GWGCWC 1 cut(s) 249
BbvI GCAGC 1 cut(s) 33
BccI CCATC 4 cut(s) 106, 166, 269, 296
BcoDI GTCTC 1 cut(s) 111
BisI GCNGC 2 cut(s) 47, 221
BlsI GCNGC 2 cut(s) 48, 222
Bme18I GGWCC 1 cut(s) 347
BmeT110I CYCGRG 2 cut(s) 54, 248
BmgT120I GGNCC 1 cut(s) 347
BmiI GGNNCC 2 cut(s) 205, 337
BmsI GCATC 1 cut(s) 219
BseGI GGATG 1 cut(s) 304
BseXI GCAGC 1 cut(s) 33
Bsh1236I CGCG 2 cut(s) 88, 223
BshNI GGYRCC 1 cut(s) 335
BsiHKAI GWGCWC 1 cut(s) 249
BsiHKCI CYCGRG 2 cut(s) 54, 248
BsiSI CCGG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 111
BsmFI GGGAC 1 cut(s) 57
BsoBI CYCGRG 2 cut(s) 54, 248
Bsp1286I GDGCHC 1 cut(s) 249
Bsp143I GATC 1 cut(s) 203
BspACI CCGC 3 cut(s) 86, 221, 384
BspFNI CGCG 2 cut(s) 88, 223
BspLI GGNNCC 2 cut(s) 205, 337
BspPI GGATC 2 cut(s) 198, 211
BspQI GCTCTTC 1 cut(s) 237
BspT107I GGYRCC 1 cut(s) 335
BssMI GATC 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 3 cut(s) 114, 184, 237
BstC8I GCNNGC 1 cut(s) 51
BstF5I GGATG 1 cut(s) 304
BstFNI CGCG 2 cut(s) 88, 223
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 1 cut(s) 206
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 1 cut(s) 203
BstMWI GCNNNNNNNGC 1 cut(s) 244
BstNSI RCATGY 1 cut(s) 322
BstUI CGCG 2 cut(s) 88, 223
BstV1I GCAGC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 203
BstYI RGATCY 1 cut(s) 203
BtsCI GGATG 1 cut(s) 304
Cac8I GCNNGC 1 cut(s) 51
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 1 cut(s) 347
Csp6I GTAC 1 cut(s) 336
CviAII CATG 2 cut(s) 184, 319
CviJI RGCY 4 cut(s) 23, 53, 247, 288
CviKI_1 RGCY 4 cut(s) 23, 53, 247, 288
CviQI GTAC 1 cut(s) 336
DpnI GATC 1 cut(s) 205
DpnII GATC 1 cut(s) 203
DrdI GACNNNNNNGTC 1 cut(s) 193
DseDI GACNNNNNNGTC 1 cut(s) 193
Eam1104I CTCTTC 3 cut(s) 114, 184, 237
EarI CTCTTC 3 cut(s) 114, 184, 237
Ecl136II GAGCTC 1 cut(s) 247
Eco24I GRGCYC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 347
Eco53kI GAGCTC 1 cut(s) 247
Eco88I CYCGRG 2 cut(s) 54, 248
EcoICRI GAGCTC 1 cut(s) 247
EcoT38I GRGCYC 1 cut(s) 249
FaeI CATG 2 cut(s) 187, 322
FaiI YATR 8 cut(s) 19, 82, 185, 269, 291, 320, 341, 406
FaqI GGGAC 1 cut(s) 57
FatI CATG 2 cut(s) 183, 318
FauI CCCGC 2 cut(s) 93, 391
Fnu4HI GCNGC 2 cut(s) 47, 221
FokI GGATG 1 cut(s) 311
FriOI GRGCYC 1 cut(s) 249
Fsp4HI GCNGC 2 cut(s) 47, 221
GlaI GCGC 1 cut(s) 323
GluI GCNGC 2 cut(s) 47, 221
HapII CCGG 1 cut(s) 201
HhaI GCGC 1 cut(s) 324
Hin1II CATG 2 cut(s) 187, 322
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HinfI GANTC 1 cut(s) 57
HpaII CCGG 1 cut(s) 201
Hpy166II GTNNAC 1 cut(s) 350
Hpy8I GTNNAC 1 cut(s) 350
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 1 cut(s) 181
HpyCH4III ACNGT 1 cut(s) 283
HpyF10VI GCNNNNNNNGC 1 cut(s) 244
Hsp92II CATG 2 cut(s) 187, 322
HspAI GCGC 1 cut(s) 322
KpnI GGTACC 1 cut(s) 339
Kzo9I GATC 1 cut(s) 203
LguI GCTCTTC 1 cut(s) 237
LpnPI CCDG 6 cut(s) 63, 149, 214, 220, 224, 404
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 1 cut(s) 219
MalI GATC 1 cut(s) 205
MboI GATC 1 cut(s) 203
MboII GAAGA 4 cut(s) 101, 171, 254, 389
MflI RGATCY 1 cut(s) 203
MhlI GDGCHC 1 cut(s) 249
MluCI AATT 4 cut(s) 14, 166, 226, 342
MlyI GAGTC 1 cut(s) 66
MnlI CCTC 4 cut(s) 151, 187, 380, 397
MseI TTAA 2 cut(s) 12, 26
MslI CAYNNNNRTG 2 cut(s) 215, 317
MspI CCGG 1 cut(s) 201
MvnI CGCG 2 cut(s) 88, 223
MwoI GCNNNNNNNGC 1 cut(s) 244
NdeII GATC 1 cut(s) 203
NlaIII CATG 2 cut(s) 187, 322
NlaIV GGNNCC 2 cut(s) 205, 337
NspI RCATGY 1 cut(s) 322
PaeR7I CTCGAG 1 cut(s) 248
PciSI GCTCTTC 1 cut(s) 237
PkrI GCNGC 2 cut(s) 48, 222
PleI GAGTC 1 cut(s) 65
PpsI GAGTC 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 249
PspN4I GGNNCC 2 cut(s) 205, 337
PspPI GGNCC 1 cut(s) 347
PspXI VCTCGAGB 1 cut(s) 248
PsuI RGATCY 1 cut(s) 203
RsaI GTAC 1 cut(s) 337
RsaNI GTAC 1 cut(s) 336
RseI CAYNNNNRTG 2 cut(s) 215, 317
SacI GAGCTC 1 cut(s) 249
SapI GCTCTTC 1 cut(s) 237
SaqAI TTAA 2 cut(s) 12, 26
SatI GCNGC 2 cut(s) 47, 221
Sau3AI GATC 1 cut(s) 203
Sau96I GGNCC 1 cut(s) 347
SchI GAGTC 1 cut(s) 66
SduI GDGCHC 1 cut(s) 249
SetI ASST 4 cut(s) 236, 249, 268, 290
SfaNI GCATC 1 cut(s) 219
Sfr274I CTCGAG 1 cut(s) 248
SinI GGWCC 1 cut(s) 347
SlaI CTCGAG 1 cut(s) 248
SmiMI CAYNNNNRTG 2 cut(s) 215, 317
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 4 cut(s) 14, 166, 226, 342
SsiI CCGC 3 cut(s) 86, 221, 384
SspI AATATT 1 cut(s) 131
SstI GAGCTC 1 cut(s) 249
TaaI ACNGT 1 cut(s) 283
TaqI TCGA 1 cut(s) 249
TasI AATT 4 cut(s) 14, 166, 226, 342
TauI GCSGC 1 cut(s) 223
Tru1I TTAA 2 cut(s) 12, 26
Tru9I TTAA 2 cut(s) 12, 26
TseI GCWGC 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 347
XapI RAATTY 1 cut(s) 226
XceI RCATGY 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.