pycom11g16500

Belongs to the hexokinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0018755
Physical Location & Seq
Reverse (-)
18087138 .. 18092551
5414 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1476 bp
ATGGGGAAGGTGGCGGTCGGATTGGCGGTTGGGGTGGCGGTGGCAGCGTGTGCGGTGGCGACGGTGGTGGTGGGTCGGAGGATAAGGAGCAGAAGGAAGTGGAGCAGAGTGGTTGGTGTTTTGAAGGACCTCGAGCAGAGCTGTGAAACCACAGTGGGGAGGTTGAGGCAGGTGGTGGACGCCATGGCGGTGGAGATGCACGCCGGTTTGGCCTCTGACGGTGGCTCGAAACTCAAAATGCTGCTCACTTTTGTGGATAAACTACCCAGTGGGGTCTTGCGGGTTAAGCTTGGAGGTACGAGGTCCTCTACTTTGGAACATGATGTGTGTCGACAAGCCATTCCTCAGGATTTGATGACTGGGACAAGCGAGGATCTATTTGATTTTATTGCTTCATCATTAAAGGAGTTTGTTGAAAGAGAGAGAGATGTTTCTAAGCCTTCACTGGACAGAAGAAGGGAGCTTGGATTTACGTTCTCTTTTCCTGTGAAACAAATTTCTGTTTCATCGGGCACTTTATTAAAATGGACAAAAGGGTTTGCGATTGAAGACATGGTTGGAAGAGAGATTGCGGGATGCTTGGAGAAAGCAATGACCAGGAAGGGTCTGAATATGAGGGTGGCAGCACTTGTCAATGACAGTGTGGGAACATTATCACTTGGACATTATCACGATACAGACACTGTTGCTGCAGTAATAATTGGAACCGGTACTAATGCCTGTTATTTGGAAAGGATAGATGCTATAATTAAGTCTCAAGGGCTCCTTACAGCATCTGGAGGCATGGTTGTCAACATGGAATGGGGAAATTTTTGGTCTTCTCATTTACCAAGAACTGTGTATGATATTGAATTAGATGCTGATAGCCCTAATCCAAATGATCAGGGTTTTGAAAAATTGATATCGGGAATGTACCTGGGCGATGTTGTGAGAAGAGTGATTGTCAGAATGTCAAAGGAGTCGGATATTTTTGAGCCTATATCTTCCAAGTTATCTATGCCTTTCATTTTGAGGACACCATTGATTGCTGCAATGCATGAGGATGACACTCCTGACTTGGAGGAAGTAGGAAGCATTTTAAGAGATAACCTTGAGATTCCTGATGCCCCTCTAAAAGTCCGAAAGCTCATAGTCAAGGTGTGTGACGTTGTGACTCGCAGGGCTGCTCGATTGGCAGCTGCTGGTATTGTAGGGATCTTGAAGAAAATTGGTCGCGATGGGAGTGGTGGAATCACCGGTGGAAGAAGTAGAAGCGATCACAGAATGAGAAGAACGGTTGTGGCGATGGAGGGGAGTTTGTATACAGGCTACACAATGTTTAGAGAGTACTTACATGAAGCATTGACGGAAATTTTAGGCGAAGATATTGCCCAGCATGTCGTACTCAAGGTTACTGAAGACGGATCAGCCATGGGAGCAGCCCTCCAGGCTGCTGCATATTCATCCTACCCTGTGGATAGCATACAGTTGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001678 GO:0003674 GO:0003824 GO:0004340 GO:0004396 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0007275 GO:0008150 GO:0008152 GO:0008865 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009266 GO:0009409 GO:0009536 GO:0009628 GO:0009651 GO:0009653 GO:0009888 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0016020 GO:0016021 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019158 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019725 GO:0019752 GO:0019867 GO:0021700 GO:0022622 GO:0030154 GO:0031090 GO:0031224 GO:0031300 GO:0031301 GO:0031306 GO:0031307 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032501 GO:0032502 GO:0032592 GO:0032787 GO:0033500 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042592 GO:0042593 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044425 GO:0044429 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0048364 GO:0048468 GO:0048469 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0048878 GO:0050896 GO:0051156 GO:0051186 GO:0051188 GO:0055082 GO:0055086 GO:0065007 GO:0065008 GO:0071695 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0080147 GO:0090407 GO:0090558 GO:0090627 GO:0098573 GO:0098588 GO:0098805 GO:0099402 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko00010 ko00051 ko00052 ko00500 ko00520 ko00521 ko00524 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04910 ko04930 ko04973 ko05230 map00010 map00051 map00052 map00500 map00520 map00521 map00524 map01100 map01110 map01120 map01130 map01200 map04066 map04910 map04930 map04973 map05230
Pfam Domains
Protein Families
Hexokinase_1 Hexokinase_2

Protein Analysis

492

Amino Acids

53.4

Weight (kDa)

7.65

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexokinase_1 PF00349 41 - 223 4.9e-50 Hexokinase
Hexokinase_2 PF03727 230 - 478 2.7e-77 Hexokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018755)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50460 AT1G50460 AT1G50460
malus_domestica MD03G1170700.v1.1 MD11G1188100.v1.1
prunus_persica Prupe.4G256200_v2.0.a1 Prupe.4G256200_v2.0.a1
pyrus_communis pycom03g12760 pycom11g16500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 160
Acc36I ACCTGC 1 cut(s) 160
AccI GTMKAC 2 cut(s) 331, 1301
AccII CGCG 1 cut(s) 1215
AciI CCGC 7 cut(s) 14, 26, 38, 53, 188, 280, 572
AclWI GGATC 3 cut(s) 381, 1202, 1411
AcsI RAATTY 3 cut(s) 495, 808, 1350
AcuI CTGAAG 1 cut(s) 1416
AcyI GRCGYC 1 cut(s) 180
AfaI GTAC 5 cut(s) 298, 712, 914, 1328, 1383
AfiI CCNNNNNNNGG 1 cut(s) 156
AgeI ACCGGT 2 cut(s) 707, 1235
AgsI TTSAA 6 cut(s) 124, 416, 548, 851, 893, 1201
AjnI CCWGG 3 cut(s) 596, 915, 1425
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AleI CACNNNNGTG 1 cut(s) 251
AluBI AGCT 5 cut(s) 141, 289, 463, 1126, 1178
AluI AGCT 5 cut(s) 141, 289, 463, 1126, 1178
Alw26I GTCTC 1 cut(s) 759
AlwI GGATC 3 cut(s) 381, 1202, 1411
AlwNI CAGNNNCTG 3 cut(s) 683, 776, 1181
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 1 cut(s) 210
ApoI RAATTY 3 cut(s) 495, 808, 1350
AsiGI ACCGGT 2 cut(s) 707, 1235
AspS9I GGNCC 2 cut(s) 127, 303
AsuHPI GGTGA 1 cut(s) 1225
AvaI CYCGRG 1 cut(s) 131
AvaII GGWCC 2 cut(s) 127, 303
AxyI CCTNAGG 1 cut(s) 345
BaeGI GKGCMC 1 cut(s) 515
BanII GRGCYC 1 cut(s) 765
BbsI GAAGAC 3 cut(s) 555, 810, 1404
BccI CCATC 2 cut(s) 1211, 1279
BcgI CGANNNNNNTGC 2 cut(s) 1349, 1383
BciT130I CCWGG 3 cut(s) 598, 917, 1427
BclI TGATCA 1 cut(s) 880
BcoDI GTCTC 1 cut(s) 759
BfmI CTRYAG 1 cut(s) 690
BfuAI ACCTGC 1 cut(s) 160
BglI GCCNNNNNGGC 2 cut(s) 209, 1427
BmcAI AGTACT 1 cut(s) 1328
Bme1390I CCNGG 3 cut(s) 598, 917, 1427
Bme18I GGWCC 2 cut(s) 127, 303
BmeT110I CYCGRG 1 cut(s) 131
BmgT120I GGNCC 2 cut(s) 127, 303
BmiI GGNNCC 2 cut(s) 706, 764
BmrFI CCNGG 3 cut(s) 598, 917, 1427
BmrI ACTGGG 2 cut(s) 261, 369
BmsI GCATC 6 cut(s) 186, 566, 730, 782, 847, 1093
BmuI ACTGGG 2 cut(s) 261, 369
BpiI GAAGAC 3 cut(s) 555, 810, 1404
BplI GAGNNNNNCTC 2 cut(s) 1407, 1439
BpmI CTGGAG 2 cut(s) 798, 1409
BpuEI CTTGAG 3 cut(s) 741, 1112, 1370
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 3 cut(s) 183, 916, 1410
BsaWI WCCGGW 2 cut(s) 707, 1235
BsaXI ACNNNNNCTCC 2 cut(s) 771, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 156
Bse118I RCCGGY 3 cut(s) 203, 707, 1235
Bse1I ACTGG 3 cut(s) 267, 364, 450
Bse21I CCTNAGG 1 cut(s) 345
Bse3DI GCAATG 2 cut(s) 597, 1038
BseBI CCWGG 3 cut(s) 598, 917, 1427
BseDI CCNNGG 3 cut(s) 183, 916, 1410
BseGI GGATG 3 cut(s) 581, 1048, 1442
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMI GCAATG 2 cut(s) 597, 1038
BseMII CTCAG 1 cut(s) 359
BseNI ACTGG 3 cut(s) 267, 364, 450
BseSI GKGCMC 1 cut(s) 515
BseYI CCCAGC 1 cut(s) 1371
Bsh1236I CGCG 1 cut(s) 1215
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 1 cut(s) 212
BshTI ACCGGT 2 cut(s) 707, 1235
BsiEI CGRYCG 1 cut(s) 18
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 204, 708, 1236
BslFI GGGAC 1 cut(s) 376
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 759
BsmFI GGGAC 1 cut(s) 376
BsnI GGCC 1 cut(s) 212
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 515, 765
Bsp143I GATC 5 cut(s) 373, 880, 1194, 1255, 1403
Bsp19I CCATGG 2 cut(s) 183, 1410
Bsp68I TCGCGA 1 cut(s) 1215
BspACI CCGC 7 cut(s) 14, 26, 38, 53, 188, 280, 572
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 1 cut(s) 358
BspFNI CGCG 1 cut(s) 1215
BspLI GGNNCC 2 cut(s) 706, 764
BspMAI CTGCAG 1 cut(s) 694
BspMI ACCTGC 1 cut(s) 160
BspPI GGATC 3 cut(s) 381, 1202, 1411
BsrDI GCAATG 2 cut(s) 597, 1038
BsrFI RCCGGY 3 cut(s) 203, 707, 1235
BsrI ACTGG 3 cut(s) 267, 364, 450
BssAI RCCGGY 3 cut(s) 203, 707, 1235
BssECI CCNNGG 3 cut(s) 183, 916, 1410
BssMI GATC 5 cut(s) 373, 880, 1194, 1255, 1403
BssNAI GTATAC 1 cut(s) 1302
BssNI GRCGYC 1 cut(s) 180
BssT1I CCWWGG 2 cut(s) 183, 1410
Bst1107I GTATAC 1 cut(s) 1302
Bst2UI CCWGG 3 cut(s) 598, 917, 1427
Bst4CI ACNGT 8 cut(s) 64, 154, 221, 641, 685, 838, 1276, 1467
Bst6I CTCTTC 2 cut(s) 556, 928
BstACI GRCGYC 1 cut(s) 180
BstAPI GCANNNNNTGC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 201
BstDEI CTNAG 2 cut(s) 345, 435
BstDSI CCRYGG 2 cut(s) 183, 1410
BstF5I GGATG 3 cut(s) 581, 1048, 1442
BstFNI CGCG 1 cut(s) 1215
BstKTI GATC 5 cut(s) 376, 883, 1197, 1258, 1406
BstMAI GTCTC 1 cut(s) 759
BstMBI GATC 5 cut(s) 373, 880, 1194, 1255, 1403
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 7 cut(s) 44, 50, 209, 286, 1172, 1415, 1427
BstNI CCWGG 3 cut(s) 598, 917, 1427
BstNSI RCATGY 1 cut(s) 1379
BstSCI CCNGG 3 cut(s) 596, 915, 1425
BstSFI CTRYAG 1 cut(s) 690
BstSLI GKGCMC 1 cut(s) 515
BstUI CGCG 1 cut(s) 1215
BstV2I GAAGAC 3 cut(s) 555, 810, 1404
BstX2I RGATCY 2 cut(s) 373, 1194
BstXI CCANNNNNNTGG 1 cut(s) 190
BstYI RGATCY 2 cut(s) 373, 1194
BstZ17I GTATAC 1 cut(s) 1302
Bsu36I CCTNAGG 1 cut(s) 345
BsuRI GGCC 1 cut(s) 212
BtgI CCRYGG 2 cut(s) 183, 1410
BtgZI GCGATG 3 cut(s) 936, 1230, 1298
BtsCI GGATG 3 cut(s) 581, 1048, 1442
BtsIMutI CAGTG 5 cut(s) 159, 274, 443, 646, 681
BtuMI TCGCGA 1 cut(s) 1215
BveI ACCTGC 1 cut(s) 160
Cac8I GCNNGC 1 cut(s) 201
CaiI CAGNNNCTG 3 cut(s) 683, 776, 1181
Cfr10I RCCGGY 3 cut(s) 203, 707, 1235
Cfr13I GGNCC 2 cut(s) 127, 303
CseI GACGC 1 cut(s) 188
Csp6I GTAC 5 cut(s) 297, 711, 913, 1327, 1382
CspAI ACCGGT 2 cut(s) 707, 1235
CviAII CATG 9 cut(s) 184, 320, 553, 784, 796, 1037, 1334, 1376, 1411
CviQI GTAC 5 cut(s) 297, 711, 913, 1327, 1382
DdeI CTNAG 2 cut(s) 345, 435
DpnI GATC 5 cut(s) 375, 882, 1196, 1257, 1405
DpnII GATC 5 cut(s) 373, 880, 1194, 1255, 1403
Eam1104I CTCTTC 2 cut(s) 556, 928
EarI CTCTTC 2 cut(s) 556, 928
Eco130I CCWWGG 2 cut(s) 183, 1410
Eco24I GRGCYC 1 cut(s) 765
Eco32I GATATC 1 cut(s) 903
Eco47I GGWCC 2 cut(s) 127, 303
Eco57I CTGAAG 1 cut(s) 1416
Eco81I CCTNAGG 1 cut(s) 345
Eco88I CYCGRG 1 cut(s) 131
EcoO109I RGGNCCY 2 cut(s) 127, 303
EcoRII CCWGG 3 cut(s) 596, 915, 1425
EcoRV GATATC 1 cut(s) 903
EcoT14I CCWWGG 2 cut(s) 183, 1410
EcoT22I ATGCAT 1 cut(s) 1038
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 2 cut(s) 183, 1410
FaeI CATG 9 cut(s) 187, 323, 556, 787, 799, 1040, 1337, 1379, 1414
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FaqI GGGAC 1 cut(s) 376
FatI CATG 9 cut(s) 183, 319, 552, 783, 795, 1036, 1333, 1375, 1410
FauI CCCGC 2 cut(s) 273, 565
FbaI TGATCA 1 cut(s) 880
FblI GTMKAC 2 cut(s) 331, 1301
FokI GGATG 3 cut(s) 588, 1055, 1429
FriOI GRGCYC 1 cut(s) 765
GsaI CCCAGC 1 cut(s) 1375
GsuI CTGGAG 2 cut(s) 798, 1409
HaeIII GGCC 1 cut(s) 212
HapII CCGG 3 cut(s) 204, 708, 1236
HgaI GACGC 1 cut(s) 188
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 9 cut(s) 187, 323, 556, 787, 799, 1040, 1337, 1379, 1414
HincII GTYRAC 2 cut(s) 332, 793
HindII GTYRAC 2 cut(s) 332, 793
HindIII AAGCTT 1 cut(s) 287
HinfI GANTC 4 cut(s) 959, 1096, 1153, 1230
HpaII CCGG 3 cut(s) 204, 708, 1236
HphI GGTGA 1 cut(s) 1225
Hpy166II GTNNAC 4 cut(s) 178, 332, 793, 1302
Hpy188I TCNGA 7 cut(s) 20, 78, 217, 609, 947, 964, 1121
Hpy188III TCNNGA 8 cut(s) 347, 671, 777, 906, 1052, 1100, 1198, 1214
Hpy8I GTNNAC 4 cut(s) 178, 332, 793, 1302
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 5 cut(s) 87, 118, 450, 450, 595
HpyCH4III ACNGT 8 cut(s) 64, 154, 221, 641, 685, 838, 1276, 1467
HpyCH4IV ACGT 2 cut(s) 473, 1146
HpyCH4V TGCA 6 cut(s) 199, 692, 1031, 1036, 1436, 1471
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 50, 209, 286, 1172, 1415, 1427
HpyF3I CTNAG 2 cut(s) 345, 435
HpySE526I ACGT 2 cut(s) 473, 1146
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 9 cut(s) 187, 323, 556, 787, 799, 1040, 1337, 1379, 1414
Ksp22I TGATCA 1 cut(s) 880
Kzo9I GATC 5 cut(s) 373, 880, 1194, 1255, 1403
LmnI GCTCC 5 cut(s) 87, 102, 460, 768, 1415
LweI GCATC 6 cut(s) 186, 566, 730, 782, 847, 1093
MaeII ACGT 2 cut(s) 473, 1146
MaeIII GTNAC 3 cut(s) 1142, 1150, 1390
MalI GATC 5 cut(s) 375, 882, 1196, 1257, 1405
MboI GATC 5 cut(s) 373, 880, 1194, 1255, 1403
MflI RGATCY 2 cut(s) 373, 1194
MhlI GDGCHC 2 cut(s) 515, 765
MluCI AATT 8 cut(s) 495, 699, 747, 808, 851, 896, 1206, 1350
MlyI GAGTC 2 cut(s) 968, 1147
MmeI TCCRAC 3 cut(s) 56, 538, 942
Mph1103I ATGCAT 1 cut(s) 1038
MseI TTAA 5 cut(s) 285, 401, 521, 750, 1079
MslI CAYNNNNRTG 3 cut(s) 188, 251, 1041
MspA1I CMGCKG 1 cut(s) 1178
MspI CCGG 3 cut(s) 204, 708, 1236
MspR9I CCNGG 3 cut(s) 598, 917, 1427
MvaI CCWGG 3 cut(s) 598, 917, 1427
MvnI CGCG 1 cut(s) 1215
MwoI GCNNNNNNNGC 7 cut(s) 44, 50, 209, 286, 1172, 1415, 1427
NcoI CCATGG 2 cut(s) 183, 1410
NdeII GATC 5 cut(s) 373, 880, 1194, 1255, 1403
NlaIII CATG 9 cut(s) 187, 323, 556, 787, 799, 1040, 1337, 1379, 1414
NlaIV GGNNCC 2 cut(s) 706, 764
NmuCI GTSAC 2 cut(s) 1142, 1150
NruI TCGCGA 1 cut(s) 1215
NsiI ATGCAT 1 cut(s) 1038
NspI RCATGY 1 cut(s) 1379
OliI CACNNNNGTG 1 cut(s) 251
PaeR7I CTCGAG 1 cut(s) 131
PaqCI CACCTGC 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 1280
PfeI GAWTC 2 cut(s) 1096, 1230
PinAI ACCGGT 2 cut(s) 707, 1235
PleI GAGTC 2 cut(s) 967, 1147
PpsI GAGTC 2 cut(s) 967, 1147
PpuMI RGGWCCY 2 cut(s) 127, 303
Psp5II RGGWCCY 2 cut(s) 127, 303
Psp6I CCWGG 3 cut(s) 596, 915, 1425
PspFI CCCAGC 1 cut(s) 1371
PspGI CCWGG 3 cut(s) 596, 915, 1425
PspN4I GGNNCC 2 cut(s) 706, 764
PspPI GGNCC 2 cut(s) 127, 303
PspPPI RGGWCCY 2 cut(s) 127, 303
PspXI VCTCGAGB 1 cut(s) 131
PstI CTGCAG 1 cut(s) 694
PstNI CAGNNNCTG 3 cut(s) 683, 776, 1181
PsuI RGATCY 2 cut(s) 373, 1194
PvuII CAGCTG 1 cut(s) 1178
RruI TCGCGA 1 cut(s) 1215
RsaI GTAC 5 cut(s) 298, 712, 914, 1328, 1383
RsaNI GTAC 5 cut(s) 297, 711, 913, 1327, 1382
RseI CAYNNNNRTG 3 cut(s) 188, 251, 1041
SalI GTCGAC 1 cut(s) 330
SaqAI TTAA 5 cut(s) 285, 401, 521, 750, 1079
Sau3AI GATC 5 cut(s) 373, 880, 1194, 1255, 1403
Sau96I GGNCC 2 cut(s) 127, 303
ScaI AGTACT 1 cut(s) 1328
SchI GAGTC 2 cut(s) 968, 1147
ScrFI CCNGG 3 cut(s) 598, 917, 1427
SduI GDGCHC 2 cut(s) 515, 765
SfaNI GCATC 6 cut(s) 186, 566, 730, 782, 847, 1093
SfcI CTRYAG 1 cut(s) 690
Sfr274I CTCGAG 1 cut(s) 131
SgrAI CRCCGGYG 1 cut(s) 1235
SinI GGWCC 2 cut(s) 127, 303
SlaI CTCGAG 1 cut(s) 131
SmiMI CAYNNNNRTG 3 cut(s) 188, 251, 1041
SmlI CTYRAG 4 cut(s) 131, 756, 1091, 1385
SmoI CTYRAG 4 cut(s) 131, 756, 1091, 1385
Sse9I AATT 8 cut(s) 495, 699, 747, 808, 851, 896, 1206, 1350
SsiI CCGC 7 cut(s) 14, 26, 38, 53, 188, 280, 572
StyD4I CCNGG 3 cut(s) 596, 915, 1425
StyI CCWWGG 2 cut(s) 183, 1410
TaaI ACNGT 8 cut(s) 64, 154, 221, 641, 685, 838, 1276, 1467
TaiI ACGT 2 cut(s) 476, 1149
TaqI TCGA 4 cut(s) 132, 227, 331, 1168
TasI AATT 8 cut(s) 495, 699, 747, 808, 851, 896, 1206, 1350
TatI WGTACW 1 cut(s) 1326
TfiI GAWTC 2 cut(s) 1096, 1230
Tru1I TTAA 5 cut(s) 285, 401, 521, 750, 1079
Tru9I TTAA 5 cut(s) 285, 401, 521, 750, 1079
TscAI CASTG 5 cut(s) 159, 274, 450, 646, 688
TseFI GTSAC 2 cut(s) 1142, 1150
Tsp45I GTSAC 2 cut(s) 1142, 1150
TspDTI ATGAA 5 cut(s) 384, 495, 994, 1350, 1431
TspGWI ACGGA 2 cut(s) 1361, 1416
TspRI CASTG 5 cut(s) 159, 274, 450, 646, 688
VpaK11BI GGWCC 2 cut(s) 127, 303
XapI RAATTY 3 cut(s) 495, 808, 1350
XceI RCATGY 1 cut(s) 1379
XhoI CTCGAG 1 cut(s) 131
XmiI GTMKAC 2 cut(s) 331, 1301
ZrmI AGTACT 1 cut(s) 1328
Zsp2I ATGCAT 1 cut(s) 1038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.