pycom12g02830

Belongs to the isocitrate and isopropylmalate dehydrogenases family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0020386
Physical Location & Seq
Reverse (-)
2569920 .. 2571772
1853 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1626 bp
ATGATTCTGCGACAATTCGTTCCAACCCCAAATTTCAAGAATGGAGAAAAAGTTGCTGCTTCAAAATTGTTGGCCAAGAAAGGCAAATACTCTTTGCTCTGTGATGTGGGTCTTTGTTCTTTTCCTAAAGTGGGTTCTTTCCTTGAAGTGGTTTTAATTTCGGTTGCAAAATCTGGTGAAAATGATGCCCACCATGTGTTTGTTGGATGGCAGCAAACTACTGATATTGCTTCTTTGAAGCATGGCTTCCATGAATTCATTCGTGACAAAGGTGGAGGTTTACCAAAAAACAAGATTTGGAAGGACCACTCAAGAATATCCAGGAATTTTTCAGAAGAAATCGACCTCTTAATTGTACCTGCTGACCAAACTCGTATTGCTCTCGAAGTGTTTGATGAAAGGTCACACTTAGCAGGTTATGATTTTCAGTTGGGCTATCACATGCCGATTTCTGACACCGTAGGTACACTTCGTAGGTTCTTTACTTCAAATGTTCAAATCAAAGATGCTCATAGTTCTAAATTTCTTGTTTGTACTGGTGAAGTCATGAAAAATGGTACTATTGTGCATTTGAAGCTTGGTTTCCATGAGTTAATTCGTGACAAGGGTGGGATAATTAGTCAGAGGCTCTTTCATTCGAAGTCCAAGAATACAGATTGTGCGCTGGGTTTTGATTGTTCCAAAGTGGTCAAATGTAAGGTGAGTTTTGATTATTCCATGATCATTAATGGGGAGTATGCTGGTAAATTGAGAGTGCATCATTTAGAAGCTTGTATGGACAAGAATAGAAAAAATGAAAGCAAGTTTACTCTCTCGAGATTTGAAACTGGTTGTTGTGTTATTTGCTATGTTGTTCTCGATCGTGACTCCTGCAAGGGCATTCGGGATAAGGGTGGTGGCTCAAAATTCAGGGTAAGGACGATGATAGTCCTAATTGTTAGGGAGGAATATCTGGTTCTTAGCTTTCACAGGCAAAAGAAGTTTCCATTTGGAAGTTCTGTTCATGGCTTTTTTGGCTTGCCAGATACTAATCATATACTTAAGGATGGTATATTAACTGGTCGAAGAATGACGACAACAAGATACCATAGTTACGAATATGATGAGCACATGAAAGTGGATGTTGCCTTCACTGGTGCAAAACACATGCCAAAACAAATGATTTACAACAGTAATTCAAAGTTCTCAATCATATCAGAGTATACGGAGGTAGTAGATGGGGCTTGGATAAGTCATGATATTGATCTCAGATTGGAGTCGAGGAAAATAAGCAGCATAAGGAAGCCTATATGGAAACTAACGAAAATGCATGTGAGTTTCCTCCATCAGAACTTGGTTAATGATCAGACAATGCAAGTCGCGGCCTGTCCTCAGGTACAATATGTATCCATCGAAGCCATTCCAAAGAGCATTGTACACAAGGAAAAAGAGCTTGAGAGGCAGAGGGAGGACCTTCTGTCCAAATCCAAGAACATTAGGGAGAGAATTGTGGAGGGGAGGATCTCAAAGAGGCTTGGGGAGCTGGCTCTTTTCGAGCAACCTTTCATTAAAGATGATAGTCTTTTGGTGAAAGACCTAGTGAAGCAGACCGTTACTGCTCTCGGTGAGAACATAAAAATTCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00020 ko00480 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 ko04146 map00020 map00480 map00720 map01100 map01110 map01120 map01130 map01200 map01210 map01230 map04146
Pfam Domains
Protein Families
Iso_dh

Protein Analysis

542

Amino Acids

61.63

Weight (kDa)

9.31

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF_TS PF00889 444 - 531 2.4e-16 Elongation factor TS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020386)

Species Orthologous Gene IDs
malus_domestica MD01G1097100.v1.1
pyrus_communis pycom09g14330 pycom12g02830 pycom13g19400 pycom13g28690 pycom15g18780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 367, 404
AccI GTMKAC 1 cut(s) 1202
AccII CGCG 1 cut(s) 1361
AciI CCGC 1 cut(s) 1361
AclWI GGATC 1 cut(s) 1508
AcoI YGGCCR 1 cut(s) 72
AcsI RAATTY 6 cut(s) 31, 254, 325, 521, 905, 1617
AdeI CACNNNGTG 1 cut(s) 196
AfaI GTAC 6 cut(s) 357, 466, 535, 559, 1377, 1416
AfiI CCNNNNNNNGG 2 cut(s) 131, 148
AflII CTTAAG 1 cut(s) 1040
AgsI TTSAA 9 cut(s) 37, 63, 146, 238, 489, 497, 574, 824, 1179
AhdI GACNNNNNGTC 1 cut(s) 1456
AjnI CCWGG 1 cut(s) 320
AloI GAACNNNNNNTCC 2 cut(s) 984, 1016
AluBI AGCT 5 cut(s) 577, 770, 963, 1432, 1522
AluI AGCT 5 cut(s) 577, 770, 963, 1432, 1522
Alw21I GWGCWC 1 cut(s) 1110
AlwI GGATC 1 cut(s) 1508
Ama87I CYCGRG 1 cut(s) 814
AoxI GGCC 2 cut(s) 72, 1362
ApeKI GCWGC 3 cut(s) 56, 211, 1272
ApoI RAATTY 6 cut(s) 31, 254, 325, 521, 905, 1617
AseI ATTAAT 1 cut(s) 726
Asp700I GAANNNNTTC 2 cut(s) 258, 1398
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 2 cut(s) 304, 1450
AsuHPI GGTGA 5 cut(s) 188, 551, 712, 1579, 1616
AsuII TTCGAA 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 814
AvaII GGWCC 2 cut(s) 304, 1450
AxyI CCTNAGG 1 cut(s) 1371
BaeI ACNNNNGTAYC 4 cut(s) 549, 582, 1075, 1108
BalI TGGCCA 1 cut(s) 74
BarI GAAGNNNNNNTAC 2 cut(s) 453, 485
Bbv12I GWGCWC 1 cut(s) 1110
BbvI GCAGC 3 cut(s) 43, 223, 1284
BccI CCATC 5 cut(s) 201, 1040, 1211, 1332, 1397
BciT130I CCWGG 1 cut(s) 322
BciVI GTATCC 1 cut(s) 1396
BclI TGATCA 2 cut(s) 720, 1342
BfaI CTAG 1 cut(s) 1577
BfmI CTRYAG 1 cut(s) 1622
BfrI CTTAAG 1 cut(s) 1040
BfuAI ACCTGC 2 cut(s) 367, 404
BfuI GTATCC 1 cut(s) 1396
BisI GCNGC 4 cut(s) 57, 212, 1273, 1362
BlsI GCNGC 4 cut(s) 58, 213, 1274, 1363
Bme1390I CCNGG 1 cut(s) 322
Bme18I GGWCC 2 cut(s) 304, 1450
BmeRI GACNNNNNGTC 1 cut(s) 1456
BmeT110I CYCGRG 1 cut(s) 814
BmgT120I GGNCC 2 cut(s) 304, 1450
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 3 cut(s) 175, 496, 766
Bpu14I TTCGAA 1 cut(s) 638
BpuEI CTTGAG 2 cut(s) 295, 1454
BsaBI GATNNNNATC 2 cut(s) 1029, 1242
BsaXI ACNNNNNCTCC 2 cut(s) 36, 66
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 148
Bse1I ACTGG 4 cut(s) 541, 832, 1063, 1138
Bse21I CCTNAGG 1 cut(s) 1371
Bse8I GATNNNNATC 2 cut(s) 1029, 1242
BseBI CCWGG 1 cut(s) 322
BseGI GGATG 3 cut(s) 212, 1051, 1126
BseJI GATNNNNATC 2 cut(s) 1029, 1242
BseLI CCNNNNNNNGG 2 cut(s) 131, 148
BseMII CTCAG 2 cut(s) 1261, 1385
BseNI ACTGG 4 cut(s) 541, 832, 1063, 1138
BseXI GCAGC 3 cut(s) 43, 223, 1284
BseYI CCCAGC 1 cut(s) 664
Bsh1236I CGCG 1 cut(s) 1361
Bsh1285I CGRYCG 1 cut(s) 862
BshFI GGCC 2 cut(s) 74, 1364
BsiEI CGRYCG 1 cut(s) 862
BsiHKAI GWGCWC 1 cut(s) 1110
BsiHKCI CYCGRG 1 cut(s) 814
BslI CCNNNNNNNGG 2 cut(s) 131, 148
BsmI GAATGC 1 cut(s) 879
BsnI GGCC 2 cut(s) 74, 1364
BsoBI CYCGRG 1 cut(s) 814
Bsp119I TTCGAA 1 cut(s) 638
Bsp1286I GDGCHC 1 cut(s) 1110
Bsp1407I TGTACA 1 cut(s) 1414
Bsp143I GATC 5 cut(s) 720, 859, 1243, 1342, 1500
BspACI CCGC 1 cut(s) 1361
BspANI GGCC 2 cut(s) 74, 1364
BspCNI CTCAG 2 cut(s) 1260, 1384
BspFNI CGCG 1 cut(s) 1361
BspHI TCATGA 2 cut(s) 546, 1234
BspMI ACCTGC 2 cut(s) 367, 404
BspPI GGATC 1 cut(s) 1508
BspT104I TTCGAA 1 cut(s) 638
BspTI CTTAAG 1 cut(s) 1040
BsrGI TGTACA 1 cut(s) 1414
BsrI ACTGG 4 cut(s) 541, 832, 1063, 1138
BssMI GATC 5 cut(s) 720, 859, 1243, 1342, 1500
BssNAI GTATAC 1 cut(s) 1203
Bst1107I GTATAC 1 cut(s) 1203
Bst2UI CCWGG 1 cut(s) 322
Bst4CI ACNGT 3 cut(s) 460, 1172, 1591
BstAFI CTTAAG 1 cut(s) 1040
BstAUI TGTACA 1 cut(s) 1414
BstBI TTCGAA 1 cut(s) 638
BstC8I GCNNGC 2 cut(s) 1019, 1524
BstDEI CTNAG 4 cut(s) 409, 959, 1247, 1371
BstF5I GGATG 3 cut(s) 212, 1051, 1126
BstFNI CGCG 1 cut(s) 1361
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 5 cut(s) 723, 862, 1246, 1345, 1503
BstMBI GATC 5 cut(s) 720, 859, 1243, 1342, 1500
BstMCI CGRYCG 1 cut(s) 862
BstMWI GCNNNNNNNGC 4 cut(s) 574, 1014, 1438, 1519
BstNI CCWGG 1 cut(s) 322
BstNSI RCATGY 3 cut(s) 445, 1150, 1313
BstSCI CCNGG 1 cut(s) 320
BstSFI CTRYAG 1 cut(s) 1622
BstUI CGCG 1 cut(s) 1361
BstV1I GCAGC 3 cut(s) 43, 223, 1284
BstX2I RGATCY 1 cut(s) 1500
BstYI RGATCY 1 cut(s) 1500
BstZ17I GTATAC 1 cut(s) 1203
Bsu36I CCTNAGG 1 cut(s) 1371
BsuI GTATCC 1 cut(s) 1396
BsuRI GGCC 2 cut(s) 74, 1364
BtsCI GGATG 3 cut(s) 212, 1051, 1126
BtsIMutI CAGTG 1 cut(s) 1131
BveI ACCTGC 2 cut(s) 367, 404
Cac8I GCNNGC 2 cut(s) 1019, 1524
CciI TCATGA 2 cut(s) 546, 1234
CfoI GCGC 1 cut(s) 664
Cfr13I GGNCC 2 cut(s) 304, 1450
Csp6I GTAC 6 cut(s) 356, 465, 534, 558, 1376, 1415
CviQI GTAC 6 cut(s) 356, 465, 534, 558, 1376, 1415
DdeI CTNAG 4 cut(s) 409, 959, 1247, 1371
DpnI GATC 5 cut(s) 722, 861, 1245, 1344, 1502
DpnII GATC 5 cut(s) 720, 859, 1243, 1342, 1500
DraIII CACNNNGTG 1 cut(s) 196
DriI GACNNNNNGTC 1 cut(s) 1456
EaeI YGGCCR 1 cut(s) 72
Eam1105I GACNNNNNGTC 1 cut(s) 1456
Eco47I GGWCC 2 cut(s) 304, 1450
Eco81I CCTNAGG 1 cut(s) 1371
Eco88I CYCGRG 1 cut(s) 814
EcoO109I RGGNCCY 1 cut(s) 1450
EcoRI GAATTC 1 cut(s) 254
EcoRII CCWGG 1 cut(s) 320
EcoT22I ATGCAT 1 cut(s) 1311
FalI AAGNNNNNCTT 2 cut(s) 230, 262
FbaI TGATCA 2 cut(s) 720, 1342
FblI GTMKAC 1 cut(s) 1202
Fnu4HI GCNGC 4 cut(s) 57, 212, 1273, 1362
FokI GGATG 3 cut(s) 219, 1058, 1133
Fsp4HI GCNGC 4 cut(s) 57, 212, 1273, 1362
FspBI CTAG 1 cut(s) 1577
GlaI GCGC 1 cut(s) 663
GluI GCNGC 4 cut(s) 57, 212, 1273, 1362
GsaI CCCAGC 1 cut(s) 668
HaeIII GGCC 2 cut(s) 74, 1364
HhaI GCGC 1 cut(s) 664
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HindIII AAGCTT 2 cut(s) 575, 768
HinfI GANTC 3 cut(s) 4, 866, 1256
HphI GGTGA 5 cut(s) 188, 551, 712, 1579, 1616
Hpy166II GTNNAC 5 cut(s) 281, 467, 807, 1203, 1417
Hpy188I TCNGA 7 cut(s) 334, 454, 624, 1198, 1250, 1329, 1347
Hpy8I GTNNAC 5 cut(s) 281, 467, 807, 1203, 1417
HpyAV CCTTC 3 cut(s) 295, 1138, 1463
HpyCH4III ACNGT 3 cut(s) 460, 1172, 1591
HpyCH4V TGCA 7 cut(s) 167, 568, 757, 873, 1139, 1309, 1354
HpyF10VI GCNNNNNNNGC 4 cut(s) 574, 1014, 1438, 1519
HpyF3I CTNAG 4 cut(s) 409, 959, 1247, 1371
HspAI GCGC 1 cut(s) 662
Ksp22I TGATCA 2 cut(s) 720, 1342
Kzo9I GATC 5 cut(s) 720, 859, 1243, 1342, 1500
LmnI GCTCC 1 cut(s) 1519
Lsp1109I GCAGC 3 cut(s) 43, 223, 1284
LweI GCATC 3 cut(s) 175, 496, 766
MaeI CTAG 1 cut(s) 1577
MaeIII GTNAC 6 cut(s) 263, 402, 599, 863, 1091, 1591
MalI GATC 5 cut(s) 722, 861, 1245, 1344, 1502
MboI GATC 5 cut(s) 720, 859, 1243, 1342, 1500
MboII GAAGA 2 cut(s) 347, 1077
MflI RGATCY 1 cut(s) 1500
MhlI GDGCHC 1 cut(s) 1110
MlsI TGGCCA 1 cut(s) 74
MluNI TGGCCA 1 cut(s) 74
MlyI GAGTC 2 cut(s) 860, 1265
MmeI TCCRAC 2 cut(s) 47, 184
Mox20I TGGCCA 1 cut(s) 74
Mph1103I ATGCAT 1 cut(s) 1311
MroXI GAANNNNTTC 2 cut(s) 258, 1398
MscI TGGCCA 1 cut(s) 74
MseI TTAA 8 cut(s) 155, 350, 593, 726, 1041, 1055, 1338, 1548
MslI CAYNNNNRTG 1 cut(s) 1115
Msp20I TGGCCA 1 cut(s) 74
MspCI CTTAAG 1 cut(s) 1040
MspR9I CCNGG 1 cut(s) 322
Mva1269I GAATGC 1 cut(s) 879
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 1 cut(s) 1361
MwoI GCNNNNNNNGC 4 cut(s) 574, 1014, 1438, 1519
NdeII GATC 5 cut(s) 720, 859, 1243, 1342, 1500
NmuCI GTSAC 4 cut(s) 263, 402, 599, 863
NsiI ATGCAT 1 cut(s) 1311
NspI RCATGY 3 cut(s) 445, 1150, 1313
NspV TTCGAA 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 814
PagI TCATGA 2 cut(s) 546, 1234
PctI GAATGC 1 cut(s) 879
PdmI GAANNNNTTC 2 cut(s) 258, 1398
PfeI GAWTC 1 cut(s) 4
PfoI TCCNGGA 1 cut(s) 320
PkrI GCNGC 4 cut(s) 58, 213, 1274, 1363
Ple19I CGATCG 1 cut(s) 862
PleI GAGTC 2 cut(s) 860, 1264
PpsI GAGTC 2 cut(s) 860, 1264
PpuMI RGGWCCY 1 cut(s) 1450
PshBI ATTAAT 1 cut(s) 726
Psp5II RGGWCCY 1 cut(s) 1450
Psp6I CCWGG 1 cut(s) 320
PspFI CCCAGC 1 cut(s) 664
PspGI CCWGG 1 cut(s) 320
PspPI GGNCC 2 cut(s) 304, 1450
PspPPI RGGWCCY 1 cut(s) 1450
PsuI RGATCY 1 cut(s) 1500
PvuI CGATCG 1 cut(s) 862
RsaI GTAC 6 cut(s) 357, 466, 535, 559, 1377, 1416
RsaNI GTAC 6 cut(s) 356, 465, 534, 558, 1376, 1415
RseI CAYNNNNRTG 1 cut(s) 1115
SaqAI TTAA 8 cut(s) 155, 350, 593, 726, 1041, 1055, 1338, 1548
SatI GCNGC 4 cut(s) 57, 212, 1273, 1362
Sau3AI GATC 5 cut(s) 720, 859, 1243, 1342, 1500
Sau96I GGNCC 2 cut(s) 304, 1450
SchI GAGTC 2 cut(s) 860, 1265
ScrFI CCNGG 1 cut(s) 322
SduI GDGCHC 1 cut(s) 1110
SfaNI GCATC 3 cut(s) 175, 496, 766
SfcI CTRYAG 1 cut(s) 1622
Sfr274I CTCGAG 1 cut(s) 814
SfuI TTCGAA 1 cut(s) 638
SinI GGWCC 2 cut(s) 304, 1450
SlaI CTCGAG 1 cut(s) 814
SmiMI CAYNNNNRTG 1 cut(s) 1115
SmlI CTYRAG 4 cut(s) 310, 814, 1040, 1433
SmoI CTYRAG 4 cut(s) 310, 814, 1040, 1433
SsiI CCGC 1 cut(s) 1361
SspMI CTAG 1 cut(s) 1577
StyD4I CCNGG 1 cut(s) 320
TaaI ACNGT 3 cut(s) 460, 1172, 1591
TaqI TCGA 9 cut(s) 342, 384, 638, 815, 858, 1063, 1259, 1392, 1533
TatI WGTACW 2 cut(s) 533, 1414
TauI GCSGC 1 cut(s) 1364
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 8 cut(s) 155, 350, 593, 726, 1041, 1055, 1338, 1548
Tru9I TTAA 8 cut(s) 155, 350, 593, 726, 1041, 1055, 1338, 1548
TscAI CASTG 1 cut(s) 1138
TseFI GTSAC 4 cut(s) 263, 402, 599, 863
TseI GCWGC 3 cut(s) 56, 211, 1272
Tsp45I GTSAC 4 cut(s) 263, 402, 599, 863
TspDTI ATGAA 9 cut(s) 247, 267, 411, 563, 623, 810, 992, 1127, 1534
TspGWI ACGGA 1 cut(s) 1220
TspRI CASTG 1 cut(s) 1138
Vha464I CTTAAG 1 cut(s) 1040
VpaK11BI GGWCC 2 cut(s) 304, 1450
VspI ATTAAT 1 cut(s) 726
XapI RAATTY 6 cut(s) 31, 254, 325, 521, 905, 1617
XceI RCATGY 3 cut(s) 445, 1150, 1313
XcmI CCANNNNNNNNNTGG 1 cut(s) 200
XhoI CTCGAG 1 cut(s) 814
XmiI GTMKAC 1 cut(s) 1202
XmnI GAANNNNTTC 2 cut(s) 258, 1398
XspI CTAG 1 cut(s) 1577
Zsp2I ATGCAT 1 cut(s) 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.