pycom12g09420

Protein of unknown function (DUF668)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010580
Physical Location & Seq
Forward (+)
11437803 .. 11438987
1185 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1185 bp
ATGGGGGGTGAGATAGTGTCTGGGTCCTGGTTTGGTAATTTGAGGTTCTCATGGAGTCGTTTGGCGGAGGCTGACAAGCCGGTGATTGGAATTCTGGCATTTGAAGTTGCGGGGTTGATGTTAAAGATGGTTAATTTATGGAATAGTGTGAGTGAGAATGAGATGCTTAGGTTGAGGGAAGAGATTGTTAACTTGATTGGGGTCAGAAGGCTCGTGTCTGACAACGATGATTACTTGATGGAACTTGCACTGAATGAGATAATTGAGGATTTGGGATATCTGGCAATGTCTGTCGTCAGGCTTGGGAAGAAGTGCACTGACCCTGTGTATCACCGTTTTGAAGAAATTTTCGAGGACCCTGTCGAGAATGGTTTTCAGTGGCTTGGATGGGCTTACAGTTGGAGCAAAATGGAGAGGAAAGTGAAGAAAATGGAGAAATTTGTTGAGGCTACAATGCAATTATCGCAGGAGCTGGAAGTGCTGGCAGAGCTTGAGCAAACTCTGAGGAGAATGCGTGCTAATCCTGAAGTAAATCGGGTAAAATTGCTTGAGTTTCAACAGAAGGTAATGTGGCGGCGTCAAGAAGTGAAAATTTTACAAGATATGTCTCCTTGGAGTAGAACGTATGATTACATTGTCCGACTTTTGGCAAGATCCCTTTTTACGATGTTAGAGAGGATAAAGCTGGTCTTTGGATTTAATCAAATGGCTTTGGGAGAGGGAATGGATAGCTCTGAAGTTATCAATTCTGTTTGTCTTTCCCGCAGTCATTCATTTTCTGCTCTTATGCACTCTTCCGTTCATCCATCTGATGGTAATCCATGTGGGTTCTATTCTGGACCTCTTGGGAGGTCGCTTACAAAGCCGAGGCTTAATGCTGGCAAGAATAGAACAAACAAGCAGCAGCAAGCTCATCACCAGTCATCCGTCCAACATGGAAGTTATTCCCATCTAAAACCCAAACGGTTTGCTCATGTTCGGTCTTTTAAAGAATGTGTGACGGGTGGAAGCAATTCTCCTGTTCTTGAGAGCTGCAAGCCAGATATGGGTGGTTCTATGAGGTTGAGAATTACCGATATCAAACATTTTGATAACTTAAAGTACACACATATGGGATCGATCCATCTTTCAGCCACGGCATCTATTCTAAACTGTCCCTGTTTAGTTCAAAATGCACACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

395

Amino Acids

45.1

Weight (kDa)

8.85

Isoelectric Point (pI)

51.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 31 - 87 1.4e-23 Domain of unknown function (DUF3475)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 812
AciI CCGC 4 cut(s) 65, 110, 574, 763
AclWI GGATC 3 cut(s) 648, 1114, 1123
AcsI RAATTY 4 cut(s) 90, 345, 437, 591
AcuI CTGAAG 2 cut(s) 546, 756
AcyI GRCGYC 1 cut(s) 577
AfaI GTAC 1 cut(s) 1103
AfiI CCNNNNNNNGG 4 cut(s) 86, 646, 812, 1046
AgsI TTSAA 4 cut(s) 104, 341, 557, 1169
AjnI CCWGG 1 cut(s) 26
AluBI AGCT 6 cut(s) 472, 490, 685, 732, 911, 1032
AluI AGCT 6 cut(s) 472, 490, 685, 732, 911, 1032
Alw21I GWGCWC 1 cut(s) 317
Alw26I GTCTC 1 cut(s) 612
Alw44I GTGCAC 1 cut(s) 313
AlwI GGATC 3 cut(s) 648, 1114, 1123
AlwNI CAGNNNCTG 1 cut(s) 472
ApaLI GTGCAC 1 cut(s) 313
ApeKI GCWGC 3 cut(s) 901, 904, 1032
ApoI RAATTY 4 cut(s) 90, 345, 437, 591
Asp700I GAANNNNTTC 2 cut(s) 943, 1012
AspS9I GGNCC 3 cut(s) 24, 355, 839
AsuHPI GGTGA 4 cut(s) 20, 94, 323, 908
AvaII GGWCC 3 cut(s) 24, 355, 839
BaeGI GKGCMC 1 cut(s) 317
BauI CACGAG 1 cut(s) 212
Bbv12I GWGCWC 1 cut(s) 317
BbvI GCAGC 3 cut(s) 913, 916, 1019
BccI CCATC 7 cut(s) 121, 232, 381, 806, 814, 957, 1131
BceAI ACGGC 1 cut(s) 1152
BciT130I CCWGG 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 612
BisI GCNGC 4 cut(s) 575, 902, 905, 1033
BlsI GCNGC 4 cut(s) 576, 903, 906, 1034
Bme1390I CCNGG 1 cut(s) 28
Bme18I GGWCC 3 cut(s) 24, 355, 839
BmgT120I GGNCC 3 cut(s) 24, 355, 839
BmiI GGNNCC 2 cut(s) 25, 357
BmrFI CCNGG 1 cut(s) 28
BmsI GCATC 2 cut(s) 153, 1148
Bpu10I CCTNAGC 1 cut(s) 167
BpuEI CTTGAG 3 cut(s) 512, 569, 1046
Bsa29I ATCGAT 1 cut(s) 1118
BsaBI GATNNNNATC 1 cut(s) 816
BsaHI GRCGYC 1 cut(s) 577
BsaJI CCNNGG 3 cut(s) 611, 866, 1134
BsaXI ACNNNNNCTCC 2 cut(s) 499, 529
Bsc4I CCNNNNNNNGG 4 cut(s) 86, 646, 812, 1046
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 1 cut(s) 919
Bse3DI GCAATG 1 cut(s) 291
Bse8I GATNNNNATC 1 cut(s) 816
BseBI CCWGG 1 cut(s) 28
BseCI ATCGAT 1 cut(s) 1118
BseDI CCNNGG 3 cut(s) 611, 866, 1134
BseGI GGATG 3 cut(s) 392, 802, 923
BseJI GATNNNNATC 1 cut(s) 816
BseLI CCNNNNNNNGG 4 cut(s) 86, 646, 812, 1046
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 494
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 1 cut(s) 520
BseSI GKGCMC 1 cut(s) 317
BseXI GCAGC 3 cut(s) 913, 916, 1019
BshVI ATCGAT 1 cut(s) 1118
BsiHKAI GWGCWC 1 cut(s) 317
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 1140
BslI CCNNNNNNNGG 4 cut(s) 86, 646, 812, 1046
BsmAI GTCTC 1 cut(s) 612
BsmFI GGGAC 1 cut(s) 1140
BsmI GAATGC 1 cut(s) 516
Bsp1286I GDGCHC 1 cut(s) 317
Bsp143I GATC 3 cut(s) 653, 1115, 1119
BspACI CCGC 4 cut(s) 65, 110, 574, 763
BspCNI CTCAG 1 cut(s) 495
BspDI ATCGAT 1 cut(s) 1118
BspLI GGNNCC 2 cut(s) 25, 357
BspPI GGATC 3 cut(s) 648, 1114, 1123
BsrDI GCAATG 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 1 cut(s) 919
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 3 cut(s) 611, 866, 1134
BssMI GATC 3 cut(s) 653, 1115, 1119
BssNI GRCGYC 1 cut(s) 577
BssSI CACGAG 1 cut(s) 212
BssT1I CCWWGG 1 cut(s) 611
Bst2BI CACGAG 1 cut(s) 212
Bst2UI CCWGG 1 cut(s) 28
Bst4CI ACNGT 4 cut(s) 335, 398, 966, 1154
Bst6I CTCTTC 2 cut(s) 174, 799
BstACI GRCGYC 1 cut(s) 577
BstC8I GCNNGC 5 cut(s) 483, 516, 880, 909, 1037
BstDEI CTNAG 2 cut(s) 167, 503
BstDSI CCRYGG 1 cut(s) 1134
BstF5I GGATG 3 cut(s) 392, 802, 923
BstKTI GATC 3 cut(s) 656, 1118, 1122
BstMAI GTCTC 1 cut(s) 612
BstMBI GATC 3 cut(s) 653, 1115, 1119
BstMWI GCNNNNNNNGC 4 cut(s) 463, 478, 487, 862
BstNI CCWGG 1 cut(s) 28
BstSCI CCNGG 1 cut(s) 26
BstSLI GKGCMC 1 cut(s) 317
BstV1I GCAGC 3 cut(s) 913, 916, 1019
BstX2I RGATCY 1 cut(s) 653
BstYI RGATCY 1 cut(s) 653
Bsu15I ATCGAT 1 cut(s) 1118
BsuTUI ATCGAT 1 cut(s) 1118
BtgI CCRYGG 1 cut(s) 1134
BtsCI GGATG 3 cut(s) 392, 802, 923
BtsIMutI CAGTG 3 cut(s) 248, 315, 383
Cac8I GCNNGC 5 cut(s) 483, 516, 880, 909, 1037
CaiI CAGNNNCTG 1 cut(s) 472
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 3 cut(s) 24, 355, 839
ClaI ATCGAT 1 cut(s) 1118
CseI GACGC 1 cut(s) 566
Csp6I GTAC 1 cut(s) 1102
CviAII CATG 4 cut(s) 51, 822, 935, 974
CviQI GTAC 1 cut(s) 1102
DdeI CTNAG 2 cut(s) 167, 503
DpnI GATC 3 cut(s) 655, 1117, 1121
DpnII GATC 3 cut(s) 653, 1115, 1119
DraI TTTAAA 1 cut(s) 988
Eam1104I CTCTTC 2 cut(s) 174, 799
EarI CTCTTC 2 cut(s) 174, 799
EciI GGCGGA 1 cut(s) 80
Eco130I CCWWGG 1 cut(s) 611
Eco32I GATATC 2 cut(s) 278, 1078
Eco47I GGWCC 3 cut(s) 24, 355, 839
Eco57I CTGAAG 2 cut(s) 546, 756
EcoO109I RGGNCCY 2 cut(s) 24, 355
EcoRI GAATTC 1 cut(s) 90
EcoRII CCWGG 1 cut(s) 26
EcoRV GATATC 2 cut(s) 278, 1078
EcoT14I CCWWGG 1 cut(s) 611
ErhI CCWWGG 1 cut(s) 611
FaeI CATG 4 cut(s) 54, 825, 938, 977
FalI AAGNNNNNCTT 2 cut(s) 674, 706
FaqI GGGAC 1 cut(s) 1140
FatI CATG 4 cut(s) 50, 821, 934, 973
FauI CCCGC 2 cut(s) 103, 770
FauNDI CATATG 1 cut(s) 1110
Fnu4HI GCNGC 4 cut(s) 575, 902, 905, 1033
FokI GGATG 3 cut(s) 399, 789, 910
Fsp4HI GCNGC 4 cut(s) 575, 902, 905, 1033
GluI GCNGC 4 cut(s) 575, 902, 905, 1033
HapII CCGG 1 cut(s) 80
HgaI GACGC 1 cut(s) 566
Hin1I GRCGYC 1 cut(s) 577
Hin1II CATG 4 cut(s) 54, 825, 938, 977
HincII GTYRAC 1 cut(s) 190
HindII GTYRAC 1 cut(s) 190
HinfI GANTC 1 cut(s) 55
HpaI GTTAAC 1 cut(s) 190
HpaII CCGG 1 cut(s) 80
HphI GGTGA 4 cut(s) 20, 94, 323, 908
Hpy166II GTNNAC 3 cut(s) 190, 315, 1104
Hpy188I TCNGA 6 cut(s) 206, 220, 504, 641, 736, 811
Hpy188III TCNNGA 5 cut(s) 364, 524, 581, 837, 1025
Hpy8I GTNNAC 3 cut(s) 190, 315, 1104
HpyAV CCTTC 2 cut(s) 201, 556
HpyCH4III ACNGT 4 cut(s) 335, 398, 966, 1154
HpyCH4IV ACGT 1 cut(s) 623
HpyCH4V TGCA 6 cut(s) 248, 315, 457, 790, 1035, 1175
HpyF10VI GCNNNNNNNGC 4 cut(s) 463, 478, 487, 862
HpyF3I CTNAG 2 cut(s) 167, 503
HpySE526I ACGT 1 cut(s) 623
Hsp92I GRCGYC 1 cut(s) 577
Hsp92II CATG 4 cut(s) 54, 825, 938, 977
KspAI GTTAAC 1 cut(s) 190
Kzo9I GATC 3 cut(s) 653, 1115, 1119
LmnI GCTCC 2 cut(s) 402, 469
Lsp1109I GCAGC 3 cut(s) 913, 916, 1019
LweI GCATC 2 cut(s) 153, 1148
MaeII ACGT 1 cut(s) 623
MaeIII GTNAC 1 cut(s) 997
MalI GATC 3 cut(s) 655, 1117, 1121
MboI GATC 3 cut(s) 653, 1115, 1119
MboII GAAGA 5 cut(s) 191, 319, 353, 436, 786
MflI RGATCY 1 cut(s) 653
MhlI GDGCHC 1 cut(s) 317
MlyI GAGTC 1 cut(s) 64
MmeI TCCRAC 3 cut(s) 380, 664, 955
MroXI GAANNNNTTC 2 cut(s) 943, 1012
MseI TTAA 7 cut(s) 122, 132, 189, 699, 873, 987, 1097
MslI CAYNNNNRTG 1 cut(s) 1109
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 1 cut(s) 28
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 1 cut(s) 28
MwoI GCNNNNNNNGC 4 cut(s) 463, 478, 487, 862
NdeI CATATG 1 cut(s) 1110
NdeII GATC 3 cut(s) 653, 1115, 1119
NlaIII CATG 4 cut(s) 54, 825, 938, 977
NlaIV GGNNCC 2 cut(s) 25, 357
NmeAIII GCCGAG 1 cut(s) 891
NmuCI GTSAC 1 cut(s) 997
PctI GAATGC 1 cut(s) 516
PdmI GAANNNNTTC 2 cut(s) 943, 1012
PflFI GACNNNGTC 1 cut(s) 359
PflMI CCANNNNNTGG 1 cut(s) 812
PkrI GCNGC 4 cut(s) 576, 903, 906, 1034
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PpuMI RGGWCCY 2 cut(s) 24, 355
Psp5II RGGWCCY 2 cut(s) 24, 355
Psp6I CCWGG 1 cut(s) 26
PspGI CCWGG 1 cut(s) 26
PspN4I GGNNCC 2 cut(s) 25, 357
PspPI GGNCC 3 cut(s) 24, 355, 839
PspPPI RGGWCCY 2 cut(s) 24, 355
PstNI CAGNNNCTG 1 cut(s) 472
PsuI RGATCY 1 cut(s) 653
PsyI GACNNNGTC 1 cut(s) 359
RsaI GTAC 1 cut(s) 1103
RsaNI GTAC 1 cut(s) 1102
RseI CAYNNNNRTG 1 cut(s) 1109
SaqAI TTAA 7 cut(s) 122, 132, 189, 699, 873, 987, 1097
SatI GCNGC 4 cut(s) 575, 902, 905, 1033
Sau3AI GATC 3 cut(s) 653, 1115, 1119
Sau96I GGNCC 3 cut(s) 24, 355, 839
SchI GAGTC 1 cut(s) 64
ScrFI CCNGG 1 cut(s) 28
SduI GDGCHC 1 cut(s) 317
SfaNI GCATC 2 cut(s) 153, 1148
SinI GGWCC 3 cut(s) 24, 355, 839
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 3 cut(s) 491, 548, 1025
SmoI CTYRAG 3 cut(s) 491, 548, 1025
SsiI CCGC 4 cut(s) 65, 110, 574, 763
StyD4I CCNGG 1 cut(s) 26
StyI CCWWGG 1 cut(s) 611
TaaI ACNGT 4 cut(s) 335, 398, 966, 1154
TaiI ACGT 1 cut(s) 626
TaqI TCGA 3 cut(s) 351, 363, 1118
TaqII GACCGA 1 cut(s) 969
TatI WGTACW 1 cut(s) 1101
TauI GCSGC 1 cut(s) 577
Tru1I TTAA 7 cut(s) 122, 132, 189, 699, 873, 987, 1097
Tru9I TTAA 7 cut(s) 122, 132, 189, 699, 873, 987, 1097
TscAI CASTG 3 cut(s) 255, 322, 383
TseFI GTSAC 1 cut(s) 997
TseI GCWGC 3 cut(s) 901, 904, 1032
Tsp45I GTSAC 1 cut(s) 997
TspDTI ATGAA 2 cut(s) 762, 791
TspGWI ACGGA 2 cut(s) 787, 916
TspRI CASTG 3 cut(s) 255, 322, 383
Tth111I GACNNNGTC 1 cut(s) 359
Van91I CCANNNNNTGG 1 cut(s) 812
VneI GTGCAC 1 cut(s) 313
VpaK11BI GGWCC 3 cut(s) 24, 355, 839
XapI RAATTY 4 cut(s) 90, 345, 437, 591
XmnI GAANNNNTTC 2 cut(s) 943, 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.