pycom12g09430

Protein of unknown function (DUF668)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010580
Physical Location & Seq
Forward (+)
11439116 .. 11439511
396 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 396 bp
ATGTTGACCACAACCATAAGAGCTGCACTGAGAGCTAGACTAAAGTCATATGCCAAAACTATGGGCTCATCTGTGTATAATCCTGCCCTTGCAGGAGAGTGGAATCGAGCAATGGAGCACATCTTGGGATGGCTTGCTCCACTCGCCCATAACATGATACGGTGGCATTCTGACCACAATATTGTGAAGCAGCAGGAAGTTTCCAAGACAAATGTGCTTCTGGTCCAGACCCTTTACTTTGCAAGTCAGGCTAAAACTGAAGATGCAATTACAGAGCTTCTCATTGGTCTGAACTATATGTGCATGGTTGACGAACTTAATAGGAAGGCTATGCGAGACGCTGGTGGATGCTGGCCATACGATGATTATATCCTCAAGCGGGATGAAATTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

132

Amino Acids

14.96

Weight (kDa)

7.8

Isoelectric Point (pI)

56.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF668 PF05003 1 - 54 7.8e-14 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 379
AcoI YGGCCR 1 cut(s) 353
AcuI CTGAAG 1 cut(s) 279
AfiI CCNNNNNNNGG 1 cut(s) 379
AluBI AGCT 3 cut(s) 23, 35, 277
AluI AGCT 3 cut(s) 23, 35, 277
Alw21I GWGCWC 1 cut(s) 120
Alw26I GTCTC 1 cut(s) 330
AoxI GGCC 1 cut(s) 353
ApeKI GCWGC 2 cut(s) 23, 190
AspS9I GGNCC 1 cut(s) 223
AvaII GGWCC 1 cut(s) 223
BalI TGGCCA 1 cut(s) 355
BanII GRGCYC 1 cut(s) 68
Bbv12I GWGCWC 1 cut(s) 120
BbvI GCAGC 2 cut(s) 10, 202
BccI CCATC 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 1 cut(s) 36
BisI GCNGC 2 cut(s) 24, 191
BlsI GCNGC 2 cut(s) 25, 192
Bme18I GGWCC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 223
BmsI GCATC 2 cut(s) 253, 338
BoxI GACNNNNGTC 1 cut(s) 43
BpuEI CTTGAG 1 cut(s) 359
Bsc4I CCNNNNNNNGG 1 cut(s) 379
Bse3DI GCAATG 1 cut(s) 117
BseGI GGATG 3 cut(s) 134, 353, 388
BseLI CCNNNNNNNGG 1 cut(s) 379
BseMI GCAATG 1 cut(s) 117
BseMII CTCAG 1 cut(s) 20
BseXI GCAGC 2 cut(s) 10, 202
BsgI GTGCAG 1 cut(s) 9
BshFI GGCC 1 cut(s) 355
BsiHKAI GWGCWC 1 cut(s) 120
BslI CCNNNNNNNGG 1 cut(s) 379
BsmAI GTCTC 1 cut(s) 330
BsmBI CGTCTC 1 cut(s) 330
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 355
Bsp1286I GDGCHC 2 cut(s) 68, 120
BspACI CCGC 1 cut(s) 379
BspANI GGCC 1 cut(s) 355
BspCNI CTCAG 1 cut(s) 21
BsrDI GCAATG 1 cut(s) 117
Bst4CI ACNGT 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 135, 353
BstDEI CTNAG 1 cut(s) 29
BstF5I GGATG 3 cut(s) 134, 353, 388
BstMAI GTCTC 1 cut(s) 330
BstMWI GCNNNNNNNGC 3 cut(s) 32, 143, 248
BstPAI GACNNNNGTC 1 cut(s) 43
BstV1I GCAGC 2 cut(s) 10, 202
BstXI CCANNNNNNTGG 1 cut(s) 61
BsuRI GGCC 1 cut(s) 355
BtsCI GGATG 3 cut(s) 134, 353, 388
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 135, 353
Cfr13I GGNCC 1 cut(s) 223
CseI GACGC 1 cut(s) 347
CviAII CATG 2 cut(s) 154, 304
CviJI RGCY 8 cut(s) 23, 35, 66, 133, 251, 277, 329, 355
CviKI_1 RGCY 8 cut(s) 23, 35, 66, 133, 251, 277, 329, 355
DdeI CTNAG 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 353
Eco24I GRGCYC 1 cut(s) 68
Eco47I GGWCC 1 cut(s) 223
Eco57I CTGAAG 1 cut(s) 279
EcoT38I GRGCYC 1 cut(s) 68
Esp3I CGTCTC 1 cut(s) 330
FaeI CATG 2 cut(s) 157, 307
FatI CATG 2 cut(s) 153, 303
FauI CCCGC 1 cut(s) 372
FauNDI CATATG 1 cut(s) 49
Fnu4HI GCNGC 2 cut(s) 24, 191
FokI GGATG 2 cut(s) 141, 360
FriOI GRGCYC 1 cut(s) 68
Fsp4HI GCNGC 2 cut(s) 24, 191
FspBI CTAG 1 cut(s) 36
GluI GCNGC 2 cut(s) 24, 191
HaeIII GGCC 1 cut(s) 355
HgaI GACGC 1 cut(s) 347
Hin1II CATG 2 cut(s) 157, 307
HincII GTYRAC 2 cut(s) 6, 310
HindII GTYRAC 2 cut(s) 6, 310
HinfI GANTC 1 cut(s) 103
Hpy166II GTNNAC 2 cut(s) 6, 310
Hpy188I TCNGA 2 cut(s) 172, 291
Hpy188III TCNNGA 1 cut(s) 226
Hpy8I GTNNAC 2 cut(s) 6, 310
HpyAV CCTTC 1 cut(s) 319
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4V TGCA 5 cut(s) 26, 92, 242, 266, 303
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 143, 248
HpyF3I CTNAG 1 cut(s) 29
Hsp92II CATG 2 cut(s) 157, 307
LmnI GCTCC 2 cut(s) 115, 142
LpnPI CCDG 8 cut(s) 78, 96, 179, 206, 233, 239, 327, 337
Lsp1109I GCAGC 2 cut(s) 10, 202
LweI GCATC 2 cut(s) 253, 338
MaeI CTAG 1 cut(s) 36
MboII GAAGA 1 cut(s) 272
MhlI GDGCHC 2 cut(s) 68, 120
MlsI TGGCCA 1 cut(s) 355
MluCI AATT 2 cut(s) 267, 387
MluNI TGGCCA 1 cut(s) 355
MnlI CCTC 1 cut(s) 383
Mox20I TGGCCA 1 cut(s) 355
MscI TGGCCA 1 cut(s) 355
MseI TTAA 2 cut(s) 318, 394
Msp20I TGGCCA 1 cut(s) 355
Mva1269I GAATGC 1 cut(s) 166
MwoI GCNNNNNNNGC 3 cut(s) 32, 143, 248
NdeI CATATG 1 cut(s) 49
NlaIII CATG 2 cut(s) 157, 307
PctI GAATGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 103
PkrI GCNGC 2 cut(s) 25, 192
PshAI GACNNNNGTC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 223
SaqAI TTAA 2 cut(s) 318, 394
SatI GCNGC 2 cut(s) 24, 191
Sau96I GGNCC 1 cut(s) 223
SduI GDGCHC 2 cut(s) 68, 120
SetI ASST 3 cut(s) 25, 37, 279
SfaNI GCATC 2 cut(s) 253, 338
SinI GGWCC 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 2 cut(s) 267, 387
SsiI CCGC 1 cut(s) 379
SspI AATATT 1 cut(s) 181
SspMI CTAG 1 cut(s) 36
TaaI ACNGT 1 cut(s) 162
TaqI TCGA 1 cut(s) 106
TasI AATT 2 cut(s) 267, 387
TfiI GAWTC 1 cut(s) 103
Tru1I TTAA 2 cut(s) 318, 394
Tru9I TTAA 2 cut(s) 318, 394
TscAI CASTG 1 cut(s) 33
TseI GCWGC 2 cut(s) 23, 190
TspRI CASTG 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 223
XspI CTAG 1 cut(s) 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.