pycom12g11710

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016548
Physical Location & Seq
Forward (+)
14094412 .. 14097220
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGTACGTGAGCCGCCCGTTGGTCCATCCGGTGGAGGCCCCGCCCACGACTGACGTGGCGGCCCAGAACGCCCTCAGAGTGAGGATGAAGGACGTCCAAGGCATGCCTGGCACCCCCGGTGGGCTCGCCCTGCGTCTCTGCCAGCTCTGCTTCGCCGCAATTTCTCTCTCCGTCATGGCCACCACCTCCGATTTTCCCACCGTCACCGCCTTCTGCTACCTTGTTGCTGCTGTTAGCTTGCAATGTGTGTGGAGTTTGTCAATGGCGATTGTTGATTTGTATGCTCTTTTGGTGAGGCGGAGTTTGCGCAATTGCAAGGCTGTGTGTTTCTTCACCATTGGAGATGGGATCACATCCACTCTTACATATGCTGCCGCATGTGCTTCTGCTGGAATCACTGTCCTCATTGCCAATGATCTTAACAAATGTGGTTTTAACCATTGCAAAAGGTTTGAGACTGCCACAGCTATGGCTTTTATCAGTTGGTTTGCTGTGTCACCAACTTTTCTTTTAAACTTCTGGTCACTGGCTTCACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

180

Amino Acids

19.29

Weight (kDa)

8.81

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 164 2.8e-26 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G37200 AT2G37200
fragaria_vesca FvH4_6g15940
malus_domestica MD12G1118500.v1.1
prunus_persica Prupe.6G236400_v2.0.a1
pyrus_communis pycom12g11710
rosa_chinensis RchiOBHm_Chr3g0469641
rosa_multiflora Rmu_sc0006483.1_g000020
rosa_roxburghii Rroxscaffold_6G00411610
rosa_rugosa Rorug03G0102800
rosa_samantha Rh3AG152500 Rh3CG167300 Rh3DG199300
rosa_wichuraiana Rw3G014380

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 96
Acc16I TGCGCA 1 cut(s) 308
AccB1I GGYRCC 1 cut(s) 110
AccB7I CCANNNNNTGG 1 cut(s) 31
AciI CCGC 7 cut(s) 13, 41, 59, 156, 207, 298, 375
AclWI GGATC 1 cut(s) 356
AcoI YGGCCR 1 cut(s) 177
AcyI GRCGYC 1 cut(s) 93
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 3 cut(s) 19, 31, 120
AjiI CACGTC 1 cut(s) 55
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 3 cut(s) 145, 237, 467
AluI AGCT 3 cut(s) 145, 237, 467
Alw26I GTCTC 2 cut(s) 140, 449
AlwI GGATC 1 cut(s) 356
AoxI GGCC 3 cut(s) 36, 60, 177
ApeKI GCWGC 2 cut(s) 227, 371
AspLEI GCGC 1 cut(s) 309
AspS9I GGNCC 3 cut(s) 22, 37, 61
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 4 cut(s) 196, 304, 325, 489
AvaII GGWCC 1 cut(s) 22
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 214, 358
BccI CCATC 2 cut(s) 33, 338
BciT130I CCWGG 1 cut(s) 108
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 2 cut(s) 140, 449
BisI GCNGC 6 cut(s) 13, 60, 156, 228, 372, 375
BlsI GCNGC 6 cut(s) 14, 61, 157, 229, 373, 376
Bme1390I CCNGG 2 cut(s) 108, 117
Bme18I GGWCC 1 cut(s) 22
BmgBI CACGTC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 22, 37, 61
BmiI GGNNCC 2 cut(s) 39, 112
BmrFI CCNGG 2 cut(s) 108, 117
BpuMI CCSGG 1 cut(s) 117
BsaAI YACGTR 1 cut(s) 7
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 97, 115
BsaWI WCCGGW 1 cut(s) 28
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 31, 120
Bse1I ACTGG 1 cut(s) 531
Bse3DI GCAATG 3 cut(s) 248, 405, 439
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 97, 115
BseGI GGATG 3 cut(s) 25, 90, 353
BseLI CCNNNNNNNGG 3 cut(s) 19, 31, 120
BseMI GCAATG 3 cut(s) 248, 405, 439
BseMII CTCAG 1 cut(s) 88
BseNI ACTGG 1 cut(s) 531
BseXI GCAGC 2 cut(s) 214, 358
BshFI GGCC 3 cut(s) 38, 62, 179
BshNI GGYRCC 1 cut(s) 110
BsiSI CCGG 2 cut(s) 29, 117
BslI CCNNNNNNNGG 3 cut(s) 19, 31, 120
BsmAI GTCTC 2 cut(s) 140, 449
BsmBI CGTCTC 1 cut(s) 140
BsnI GGCC 3 cut(s) 38, 62, 179
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 348, 415
BspACI CCGC 7 cut(s) 13, 41, 59, 156, 207, 298, 375
BspANI GGCC 3 cut(s) 38, 62, 179
BspCNI CTCAG 1 cut(s) 87
BspLI GGNNCC 2 cut(s) 39, 112
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 110
BsrDI GCAATG 3 cut(s) 248, 405, 439
BsrI ACTGG 1 cut(s) 531
BssECI CCNNGG 2 cut(s) 97, 115
BssMI GATC 2 cut(s) 348, 415
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 97
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 202, 400, 537
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 7
BstC8I GCNNGC 4 cut(s) 104, 126, 143, 239
BstDEI CTNAG 1 cut(s) 74
BstF5I GGATG 3 cut(s) 25, 90, 353
BstHHI GCGC 1 cut(s) 309
BstKTI GATC 2 cut(s) 351, 418
BstMAI GTCTC 2 cut(s) 140, 449
BstMBI GATC 2 cut(s) 348, 415
BstMWI GCNNNNNNNGC 6 cut(s) 68, 108, 130, 147, 304, 380
BstNI CCWGG 1 cut(s) 108
BstNSI RCATGY 2 cut(s) 106, 381
BstSCI CCNGG 2 cut(s) 106, 115
BstV1I GCAGC 2 cut(s) 214, 358
BstXI CCANNNNNNTGG 1 cut(s) 469
BsuRI GGCC 3 cut(s) 38, 62, 179
BtrI CACGTC 1 cut(s) 55
BtsCI GGATG 3 cut(s) 25, 90, 353
BtsIMutI CAGTG 2 cut(s) 396, 524
Cac8I GCNNGC 4 cut(s) 104, 126, 143, 239
CfoI GCGC 1 cut(s) 309
Cfr13I GGNCC 3 cut(s) 22, 37, 61
CseI GACGC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 230, 265
CviAII CATG 3 cut(s) 103, 175, 378
CviQI GTAC 1 cut(s) 4
DdeI CTNAG 1 cut(s) 74
DpnI GATC 2 cut(s) 350, 417
DpnII GATC 2 cut(s) 348, 415
DraI TTTAAA 1 cut(s) 513
EaeI YGGCCR 1 cut(s) 177
EciI GGCGGA 1 cut(s) 313
Eco130I CCWWGG 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 126
Eco47I GGWCC 1 cut(s) 22
EcoO109I RGGNCCY 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 97
EcoT38I GRGCYC 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 97
Esp3I CGTCTC 1 cut(s) 140
FaeI CATG 3 cut(s) 106, 178, 381
FaiI YATR 7 cut(s) 104, 176, 282, 367, 369, 379, 470
FatI CATG 3 cut(s) 102, 174, 377
FauI CCCGC 1 cut(s) 48
FauNDI CATATG 1 cut(s) 367
Fnu4HI GCNGC 6 cut(s) 13, 60, 156, 228, 372, 375
FokI GGATG 3 cut(s) 12, 97, 340
FriOI GRGCYC 1 cut(s) 126
Fsp4HI GCNGC 6 cut(s) 13, 60, 156, 228, 372, 375
FspI TGCGCA 1 cut(s) 308
GlaI GCGC 1 cut(s) 308
GluI GCNGC 6 cut(s) 13, 60, 156, 228, 372, 375
HaeIII GGCC 3 cut(s) 38, 62, 179
HapII CCGG 2 cut(s) 29, 117
HgaI GACGC 1 cut(s) 122
HhaI GCGC 1 cut(s) 309
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 3 cut(s) 106, 178, 381
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HinfI GANTC 1 cut(s) 393
HpaII CCGG 2 cut(s) 29, 117
HphI GGTGA 4 cut(s) 196, 304, 325, 489
Hpy188I TCNGA 2 cut(s) 77, 190
HpyAV CCTTC 2 cut(s) 82, 220
HpyCH4III ACNGT 3 cut(s) 202, 400, 537
HpyCH4IV ACGT 3 cut(s) 6, 54, 93
HpyCH4V TGCA 3 cut(s) 241, 315, 444
HpyF10VI GCNNNNNNNGC 6 cut(s) 68, 108, 130, 147, 304, 380
HpyF3I CTNAG 1 cut(s) 74
HpySE526I ACGT 3 cut(s) 6, 54, 93
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 3 cut(s) 106, 178, 381
HspAI GCGC 1 cut(s) 307
Kzo9I GATC 2 cut(s) 348, 415
Lsp1109I GCAGC 2 cut(s) 214, 358
MaeII ACGT 3 cut(s) 6, 54, 93
MaeIII GTNAC 3 cut(s) 202, 495, 522
MalI GATC 2 cut(s) 350, 417
MboI GATC 2 cut(s) 348, 415
MboII GAAGA 1 cut(s) 322
MfeI CAATTG 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 126
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 2 cut(s) 159, 310
MluNI TGGCCA 1 cut(s) 179
MnlI CCTC 6 cut(s) 28, 75, 83, 196, 288, 413
Mox20I TGGCCA 1 cut(s) 179
MscI TGGCCA 1 cut(s) 179
MseI TTAA 3 cut(s) 420, 435, 512
MslI CAYNNNNRTG 1 cut(s) 467
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 2 cut(s) 29, 117
MspR9I CCNGG 2 cut(s) 108, 117
MunI CAATTG 1 cut(s) 310
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 6 cut(s) 68, 108, 130, 147, 304, 380
NciI CCSGG 1 cut(s) 117
NdeI CATATG 1 cut(s) 367
NdeII GATC 2 cut(s) 348, 415
NlaIII CATG 3 cut(s) 106, 178, 381
NlaIV GGNNCC 2 cut(s) 39, 112
NmuCI GTSAC 3 cut(s) 202, 495, 522
NsbI TGCGCA 1 cut(s) 308
NspI RCATGY 2 cut(s) 106, 381
PaeI GCATGC 1 cut(s) 106
PfeI GAWTC 1 cut(s) 393
PflMI CCANNNNNTGG 1 cut(s) 31
PkrI GCNGC 6 cut(s) 14, 61, 157, 229, 373, 376
Ppu21I YACGTR 1 cut(s) 7
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PspN4I GGNNCC 2 cut(s) 39, 112
PspPI GGNCC 3 cut(s) 22, 37, 61
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
RseI CAYNNNNRTG 1 cut(s) 467
SaqAI TTAA 3 cut(s) 420, 435, 512
SatI GCNGC 6 cut(s) 13, 60, 156, 228, 372, 375
Sau3AI GATC 2 cut(s) 348, 415
Sau96I GGNCC 3 cut(s) 22, 37, 61
ScrFI CCNGG 2 cut(s) 108, 117
SduI GDGCHC 1 cut(s) 126
SetI ASST 9 cut(s) 9, 57, 96, 147, 188, 222, 239, 452, 469
SinI GGWCC 1 cut(s) 22
SmiMI CAYNNNNRTG 1 cut(s) 467
SphI GCATGC 1 cut(s) 106
Sse9I AATT 2 cut(s) 159, 310
SsiI CCGC 7 cut(s) 13, 41, 59, 156, 207, 298, 375
StyD4I CCNGG 2 cut(s) 106, 115
StyI CCWWGG 1 cut(s) 97
TaaI ACNGT 3 cut(s) 202, 400, 537
TaiI ACGT 3 cut(s) 9, 57, 96
TasI AATT 2 cut(s) 159, 310
TauI GCSGC 4 cut(s) 15, 62, 158, 377
TfiI GAWTC 1 cut(s) 393
Tru1I TTAA 3 cut(s) 420, 435, 512
Tru9I TTAA 3 cut(s) 420, 435, 512
TscAI CASTG 2 cut(s) 403, 531
TseFI GTSAC 3 cut(s) 202, 495, 522
TseI GCWGC 2 cut(s) 227, 371
Tsp45I GTSAC 3 cut(s) 202, 495, 522
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 2 cut(s) 403, 531
Van91I CCANNNNNTGG 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 22
XceI RCATGY 2 cut(s) 106, 381
XcmI CCANNNNNNNNNTGG 2 cut(s) 52, 104
ZraI GACGTC 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.