pycom12g12730

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0021183
Physical Location & Seq
Reverse (-)
15173762 .. 15174364
603 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 603 bp
ATGTCGATTCTACTCCTCTTAGCCTTCTTGGTAGCAATCACTCCCGCCATCGAAGGCGTCTGCTCTGACGCCATGCTCACTTCCCTCTCGTCTTCCGGCTTCACAATCTTCGCCCACGCTCTACATTCCCAAAACATCACCGCCACCGCCACCACCGCCGCCAGAGTGACTTGCTTCGTGCCACCCGACGACTCCCTCTTAGGCAAGGTGCTCGGCCCCACCACCCTCCGCGCTCACGTCTACACCTCCGGCGCCCTGCCTTACCAAGCCCTCCTCAAGCTCCCGCTCAACACCACCCTCCCTGCTCACAACACACGGCTTGTTTTCGGCACCGACGGCAGAAGAGTCTCGTTCAACGACGTTCTTGTCACCGCCCCGAATCTCCATGTCGACGATTCTTGCGTTGTTCACGGCGTTGAAGGGCCGCTGGTGCCAATGCCTCCATTGACAGATGATGCTGCGAGGCCAAGAACCAAGAATGTGGCGTCTCCGGCATTGACAAACCACCGTCGCCGGCGGTTTGCTGAGATTGTCAGGTTTATCCGTCGTGCACACCGCCCGATTTTCATTGACGGGGATCAAGACACAACCAGCCTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

21.55

Weight (kDa)

9.02

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0021183)

Species Orthologous Gene IDs
malus_domestica MD04G1109900.v1.1
pyrus_communis pycom04g10610 pycom12g12730
rosa_multiflora Rmu_sc0006706.1_g000007
rosa_roxburghii Rroxscaffold_6G00413190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 365
AccB1I GGYRCC 3 cut(s) 251, 329, 430
AccBSI CCGCTC 1 cut(s) 286
AccI GTMKAC 2 cut(s) 240, 390
AccII CGCG 1 cut(s) 231
AclWI GGATC 1 cut(s) 585
AcyI GRCGYC 4 cut(s) 57, 69, 252, 485
AgsI TTSAA 2 cut(s) 355, 419
AjiI CACGTC 1 cut(s) 238
AluBI AGCT 1 cut(s) 280
AluI AGCT 1 cut(s) 280
Alw21I GWGCWC 2 cut(s) 213, 553
Alw26I GTCTC 2 cut(s) 352, 492
Alw44I GTGCAC 1 cut(s) 549
AlwI GGATC 1 cut(s) 585
AoxI GGCC 3 cut(s) 214, 422, 464
ApaLI GTGCAC 1 cut(s) 549
ApeKI GCWGC 1 cut(s) 458
AspLEI GCGC 2 cut(s) 233, 254
AspS9I GGNCC 2 cut(s) 215, 422
AsuHPI GGTGA 2 cut(s) 130, 361
BaeGI GKGCMC 1 cut(s) 553
BanI GGYRCC 3 cut(s) 251, 329, 430
BbsI GAAGAC 1 cut(s) 84
Bbv12I GWGCWC 2 cut(s) 213, 553
BbvI GCAGC 1 cut(s) 445
BccI CCATC 1 cut(s) 56
BceAI ACGGC 3 cut(s) 332, 352, 427
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BcoDI GTCTC 2 cut(s) 352, 492
BfoI RGCGCY 1 cut(s) 255
BisI GCNGC 3 cut(s) 159, 425, 459
BlsI GCNGC 3 cut(s) 160, 426, 460
BmgBI CACGTC 1 cut(s) 238
BmgT120I GGNCC 2 cut(s) 215, 422
BmiI GGNNCC 4 cut(s) 217, 253, 331, 432
BmsI GCATC 1 cut(s) 445
BpiI GAAGAC 1 cut(s) 84
BpuEI CTTGAG 1 cut(s) 260
BsaHI GRCGYC 4 cut(s) 57, 69, 252, 485
Bse118I RCCGGY 1 cut(s) 513
BseMII CTCAG 1 cut(s) 516
BseRI GAGGAG 2 cut(s) 5, 263
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 1 cut(s) 445
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 3 cut(s) 216, 424, 466
BshNI GGYRCC 3 cut(s) 251, 329, 430
BsiHKAI GWGCWC 2 cut(s) 213, 553
BsiSI CCGG 4 cut(s) 96, 249, 491, 514
BsmAI GTCTC 2 cut(s) 352, 492
BsmBI CGTCTC 1 cut(s) 492
BsnI GGCC 3 cut(s) 216, 424, 466
Bsp1286I GDGCHC 2 cut(s) 213, 553
Bsp143I GATC 1 cut(s) 577
BspANI GGCC 3 cut(s) 216, 424, 466
BspCNI CTCAG 1 cut(s) 517
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 4 cut(s) 217, 253, 331, 432
BspPI GGATC 1 cut(s) 585
BspT107I GGYRCC 3 cut(s) 251, 329, 430
BsrBI CCGCTC 1 cut(s) 286
BsrFI RCCGGY 1 cut(s) 513
BssAI RCCGGY 1 cut(s) 513
BssMI GATC 1 cut(s) 577
BssNI GRCGYC 4 cut(s) 57, 69, 252, 485
Bst4CI ACNGT 1 cut(s) 509
Bst6I CTCTTC 1 cut(s) 337
BstACI GRCGYC 4 cut(s) 57, 69, 252, 485
BstC8I GCNNGC 1 cut(s) 515
BstDEI CTNAG 3 cut(s) 19, 199, 525
BstFNI CGCG 1 cut(s) 231
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 2 cut(s) 233, 254
BstKTI GATC 1 cut(s) 580
BstMAI GTCTC 2 cut(s) 352, 492
BstMBI GATC 1 cut(s) 577
BstMWI GCNNNNNNNGC 4 cut(s) 155, 336, 430, 491
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 231
BstV1I GCAGC 1 cut(s) 445
BstV2I GAAGAC 1 cut(s) 84
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 3 cut(s) 216, 424, 466
BtrI CACGTC 1 cut(s) 238
Cac8I GCNNGC 1 cut(s) 515
CfoI GCGC 2 cut(s) 233, 254
Cfr10I RCCGGY 1 cut(s) 513
Cfr13I GGNCC 2 cut(s) 215, 422
CseI GACGC 3 cut(s) 46, 77, 474
CviAII CATG 2 cut(s) 73, 386
CviJI RGCY 9 cut(s) 23, 99, 216, 269, 280, 319, 424, 466, 594
CviKI_1 RGCY 9 cut(s) 23, 99, 216, 269, 280, 319, 424, 466, 594
DdeI CTNAG 3 cut(s) 19, 199, 525
DinI GGCGCC 1 cut(s) 253
DpnI GATC 1 cut(s) 579
DpnII GATC 1 cut(s) 577
DrdI GACNNNNNNGTC 1 cut(s) 365
DseDI GACNNNNNNGTC 1 cut(s) 365
Eam1104I CTCTTC 1 cut(s) 337
EarI CTCTTC 1 cut(s) 337
EgeI GGCGCC 1 cut(s) 253
EheI GGCGCC 1 cut(s) 253
Esp3I CGTCTC 1 cut(s) 492
FaeI CATG 2 cut(s) 76, 389
FaiI YATR 2 cut(s) 74, 387
FatI CATG 2 cut(s) 72, 385
FauI CCCGC 2 cut(s) 52, 291
FblI GTMKAC 2 cut(s) 240, 390
Fnu4HI GCNGC 3 cut(s) 159, 425, 459
Fsp4HI GCNGC 3 cut(s) 159, 425, 459
GlaI GCGC 2 cut(s) 232, 253
GluI GCNGC 3 cut(s) 159, 425, 459
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 3 cut(s) 216, 424, 466
HapII CCGG 4 cut(s) 96, 249, 491, 514
HgaI GACGC 3 cut(s) 46, 77, 474
HhaI GCGC 2 cut(s) 233, 254
Hin1I GRCGYC 4 cut(s) 57, 69, 252, 485
Hin1II CATG 2 cut(s) 76, 389
Hin6I GCGC 2 cut(s) 231, 252
HinP1I GCGC 2 cut(s) 231, 252
HincII GTYRAC 1 cut(s) 391
HindII GTYRAC 1 cut(s) 391
HinfI GANTC 5 cut(s) 7, 191, 345, 379, 395
HpaII CCGG 4 cut(s) 96, 249, 491, 514
HphI GGTGA 2 cut(s) 130, 361
Hpy166II GTNNAC 4 cut(s) 241, 391, 409, 551
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 1 cut(s) 581
Hpy8I GTNNAC 4 cut(s) 241, 391, 409, 551
Hpy99I CGWCG 6 cut(s) 191, 338, 362, 395, 513, 549
HpyAV CCTTC 3 cut(s) 34, 47, 413
HpyCH4III ACNGT 1 cut(s) 509
HpyCH4IV ACGT 2 cut(s) 237, 360
HpyCH4V TGCA 1 cut(s) 551
HpyF10VI GCNNNNNNNGC 4 cut(s) 155, 336, 430, 491
HpyF3I CTNAG 3 cut(s) 19, 199, 525
HpySE526I ACGT 2 cut(s) 237, 360
Hsp92I GRCGYC 4 cut(s) 57, 69, 252, 485
Hsp92II CATG 2 cut(s) 76, 389
HspAI GCGC 2 cut(s) 231, 252
KasI GGCGCC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 513
KroNI GCCGGC 1 cut(s) 515
Kzo9I GATC 1 cut(s) 577
LmnI GCTCC 1 cut(s) 285
LpnPI CCDG 9 cut(s) 109, 175, 262, 269, 315, 413, 504, 520, 527
Lsp1109I GCAGC 1 cut(s) 445
LweI GCATC 1 cut(s) 445
MaeII ACGT 2 cut(s) 237, 360
MaeIII GTNAC 2 cut(s) 166, 367
MalI GATC 1 cut(s) 579
MbiI CCGCTC 1 cut(s) 286
MboI GATC 1 cut(s) 577
MboII GAAGA 3 cut(s) 84, 100, 354
MhlI GDGCHC 2 cut(s) 213, 553
Mly113I GGCGCC 1 cut(s) 252
MlyI GAGTC 2 cut(s) 185, 354
MreI CGCCGGCG 1 cut(s) 513
MroNI GCCGGC 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 427
MspI CCGG 4 cut(s) 96, 249, 491, 514
MvnI CGCG 1 cut(s) 231
MwoI GCNNNNNNNGC 4 cut(s) 155, 336, 430, 491
NaeI GCCGGC 1 cut(s) 515
NarI GGCGCC 1 cut(s) 252
NdeII GATC 1 cut(s) 577
NgoMIV GCCGGC 1 cut(s) 513
NlaIII CATG 2 cut(s) 76, 389
NlaIV GGNNCC 4 cut(s) 217, 253, 331, 432
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 166, 367
PcsI WCGNNNNNNNCGW 2 cut(s) 183, 399
PdiI GCCGGC 1 cut(s) 515
PfeI GAWTC 3 cut(s) 7, 379, 395
PkrI GCNGC 3 cut(s) 160, 426, 460
PleI GAGTC 2 cut(s) 185, 353
PluTI GGCGCC 1 cut(s) 255
PpsI GAGTC 2 cut(s) 185, 353
PspN4I GGNNCC 4 cut(s) 217, 253, 331, 432
PspPI GGNCC 2 cut(s) 215, 422
SalI GTCGAC 1 cut(s) 389
SatI GCNGC 3 cut(s) 159, 425, 459
Sau3AI GATC 1 cut(s) 577
Sau96I GGNCC 2 cut(s) 215, 422
SchI GAGTC 2 cut(s) 185, 354
SduI GDGCHC 2 cut(s) 213, 553
SetI ASST 6 cut(s) 210, 240, 248, 282, 363, 539
SfaNI GCATC 1 cut(s) 445
SfoI GGCGCC 1 cut(s) 253
SgrAI CRCCGGYG 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
SspDI GGCGCC 1 cut(s) 251
TaaI ACNGT 1 cut(s) 509
TaiI ACGT 2 cut(s) 240, 363
TaqI TCGA 3 cut(s) 5, 51, 390
TauI GCSGC 2 cut(s) 161, 427
TfiI GAWTC 3 cut(s) 7, 379, 395
TseFI GTSAC 2 cut(s) 166, 367
TseI GCWGC 1 cut(s) 458
Tsp45I GTSAC 2 cut(s) 166, 367
TspDTI ATGAA 1 cut(s) 556
TspGWI ACGGA 1 cut(s) 533
VneI GTGCAC 1 cut(s) 549
XmiI GTMKAC 2 cut(s) 240, 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.