Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0005294

Physical Location & Seq

Genomic Coordinates Reverse (-)

15348737 .. 15351006

Gene Structure 2270 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g12890.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGAGTCGGTCAAGTACGTGCCTTGGTATTATATATGATGACTCCCACCAGTTTCAAAAGTCAAATCCCACGCTCACCATCTCCCTCTTATATATACCCCTCCCACTCTCCAGTCCCAACCATAACCCCAACACAATCACAATATCCCTCTCCCCTCCAAATCCCACACACAACACCCACCAAATTTTCTCGAGAAAATCTAATCCAGTCCAATCCAAGCCAATAGGATTTTCAGAATTGAAAATGCCAATAAAAGGAATAAGGAGCTTGTGCTTACACTCCAAAACGCCGTCGTTGGCGGCCTACTCTTCTTCCCCTTCCAGACTATCGGCCTACTCTTCTTCCCCTTCTAGGCCTTCCGTTTCATTTCCAACCCCGCGGCGCTTTCCCGTGGAGAACTTCACGGACGCGATTAACCAGCAGATCATCGACAACGCCGCCGTATTGGTCATGAAGTGGAACCCGGACACCTCCACGTACGCCAAAGTCACTTCCTTATTCTACGAGAGCAAAAACGAAGCCCGGCAGTTTATCCGCTGCGTGACGGACCTCCAGATTGCCATGCACTACCTCGTCCAGGAGGACCCCACCTCAGAAAAACTCGTTCAGGCGCAGAGCCTCATGCAGGTGGCAATGAAGCGCCTCCAGAAAGAGTTCTACCAGATCCTCTCCATGAACCGGGAGCACCTCGACCCAGAATCAGTCTCCACCCGGTCCAGGTCCTCTATCGCCTCGACCCGGTCCAGCATATCTGATGACGACGAAGACCGCGGCGCCACCACGGACGACGACGTAAAATTCGCCACAGACGCCATCCTCGAAGTCGAACAGGTCTCGGCCATCGCTATGAACGACTTGAAATTAATCGCCGAATGCATGATCACATCGGGATATTCGAAGGAATGCGTCCACATCTACAAAATCATCAGAAAATCGATTATCGACGAGGGAATCTACAAGCTCGGCGTCGAGCGGTTCAGCTCGTCGCAGATCGGGAAGATGGACCGGGAGGTTCTGGATCTGAGATACAAGAGCTGGTTGAACACGGTGAAGATCTCGATCTCCACGCTCTTCAATGGCGAGCGAATCCTCTGCGACCACGTGTTCTCATCCTCTGCCTCGATCCGCGAGTCCTGCTTCACGGACATTTCTCGCGAGGCGGCGACTTTGCTCTTCGGCTTCCCGCAAGTTCTGGTGGCGGTGAAGAGCAAGAAGAACTCTCTCGACATTTTCCGCCTCCTCGACATGTACACTGCGATCTCCGAGAACTGGCCGGAGATCGAATCCATCTTCGGCTTTGAATCCACGGCTGCCGTCCGTTCTCAGGCCCTCAACTCGCTCATCAAACTCAGCGAATCGGTGCTATCTCTGCTCTCCGATTTCGAGTCCATGGTCCAGAAAGACTCGTCGAAATCCGTCCCCACCGGCGGCGGGATCCACGATCTGACTCTCCGCGCGATGAATTACCTCTCGCTCCTCACCGATTACAGCAACGTCCTCGCTGACATTATTACCGACTGGCCTCCGCCGGCGAAATCATCCCATCCAAAATCATACTTCGACAGCCCCGTCTCCGACGAAGCTCCTGCACCGGCCATCTCCGCCCGCATGGCGTGGCTCGTCCTCGTCCTCCTCTGCAAGCTCAACGACAAGGCCAAGCACTACAAGGATGTCGCACTCTCGTACCTCTTCCTGGCCAACAATCTCCAGCACGTAATCTCGAAAGTCCGTACGTCGAATCTCCAGTACCTCCTTGGCGAGGACTGGATTTCGAAGCACGAGGCGAAAGTGCGGCAGTTCGCGGCGAACTACGAGAAATTGGCTTGGGAGGAAGTGTTATCTTCGCTGCCGGAGAACCCGACGGCTCAGATTTGTCCCGAGGAAGCGAGATTGATCTTCCGCAACTTCAATTTCGCGTTTGACGAAGCCTATCGGAAACAAAGGACGAGTGTCGTACCCGACCCAAAACTCCGAGACGATATTAAAGTTTCGGTAGGGCAAAAGCTCGTTCCGGTTTACCGGGAGTTTTACGAAGCGCACAGGATGACGGTCGGGGGCGGGAGAAGCGGGGCGCTTTATGCCAGAATTGCCCCTGAAGATGTTGGAAATCACTTATCCGACCTGTTTCTTCCACCGTCGTCGTTGTCTCATCGGCGGAGCCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04910 map04910

Pfam Domains

Protein Families

Exo70

Protein Analysis

722

Amino Acids

80.9

Weight (kDa)

7.28

Isoelectric Point (pI)

51.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Exo70_N	PF20669	143 - 224	3.2e-18	Exocyst complex component Exo70 N-terminal
Exo70_C	PF03081	344 - 707	3e-107	Exo70 exocyst complex subunit C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09520
fragaria_vesca	FvH4_6g14650 FvH4_6g14670
malus_domestica	MD04G1117800.v1.1 MD12G1133300.v1.1
prunus_persica	Prupe.6G247200_v2.0.a1
pyrus_communis	pycom04g10770 pycom12g12890
rosa_chinensis	RchiOBHm_Chr3g0467831 RchiOBHm_Chr3g0467851
rosa_laevigata	RLG00000024472 RLG00000024473
rosa_multiflora	Rmu_sc0001793.1_g000029 Rmu_sc0001793.1_g000033 Rmu_sc0028415.1_g000003
rosa_roxburghii	Rroxscaffold_6G00413420 Rroxscaffold_6G00413430
rosa_rugosa	Rorug03G0089800 Rorug03G0089900
rosa_samantha	Rh3AG138200 Rh3AG138300 Rh3BG159600 Rh3BG159700 Rh3CG159600 Rh3CG159700 Rh3DG160100 Rh3DG160300
rosa_wichuraiana	Rw3G013090 Rw3G013110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	616
AasI	GACNNNNNNGTC	1 cut(s)	1568
Acc36I	ACCTGC	1 cut(s)	616
AccB1I	GGYRCC	1 cut(s)	773
AccBSI	CCGCTC	1 cut(s)	973
AccII	CGCG	9 cut(s)	379, 410, 771, 1128, 1155, 1455, 1457, 1802, 1916
AclWI	GGATC	5 cut(s)	658, 1026, 1117, 1429, 1442
AcoI	YGGCCR	4 cut(s)	837, 1271, 1593, 1695
AcsI	RAATTY	2 cut(s)	183, 797
AcuI	CTGAAG	1 cut(s)	2115
AcvI	CACGTG	1 cut(s)	1102
AcyI	GRCGYC	3 cut(s)	774, 810, 966
AfaI	GTAC	7 cut(s)	16, 479, 1250, 1685, 1732, 1748, 1956
AflIII	ACRYGT	2 cut(s)	1101, 1245
AgsI	TTSAA	7 cut(s)	56, 241, 859, 1042, 1075, 1301, 1909
AjnI	CCWGG	3 cut(s)	576, 716, 1692
AjuI	GAANNNNNNNTTGG	2 cut(s)	214, 246
AluBI	AGCT	7 cut(s)	267, 961, 981, 1035, 1583, 1642, 2005
AluI	AGCT	7 cut(s)	267, 961, 981, 1035, 1583, 1642, 2005
Alw21I	GWGCWC	1 cut(s)	687
Alw26I	GTCTC	5 cut(s)	709, 838, 1576, 1968, 2151
AlwI	GGATC	5 cut(s)	658, 1026, 1117, 1429, 1442
Ama87I	CYCGRG	2 cut(s)	190, 1877
ApeKI	GCWGC	3 cut(s)	537, 1310, 1846
ApoI	RAATTY	2 cut(s)	183, 797
ArsI	GACNNNNNNTTYG	2 cut(s)	782, 814
AseI	ATTAAT	1 cut(s)	863
Asp700I	GAANNNNTTC	1 cut(s)	653
AspLEI	GCGC	7 cut(s)	384, 613, 642, 776, 1457, 2038, 2074
AspS9I	GGNCC	8 cut(s)	547, 583, 714, 720, 741, 1003, 1327, 1393
AsuC2I	CCSGG	7 cut(s)	464, 523, 680, 712, 739, 1007, 2021
AsuHPI	GGTGA	4 cut(s)	67, 1060, 1213, 1471
AsuII	TTCGAA	2 cut(s)	896, 1772
AvaI	CYCGRG	2 cut(s)	190, 1877
AvaII	GGWCC	7 cut(s)	547, 583, 714, 720, 741, 1003, 1393
BaeI	ACNNNNGTAYC	2 cut(s)	1667, 1700
BalI	TGGCCA	1 cut(s)	1697
BamHI	GGATCC	1 cut(s)	1434
BanI	GGYRCC	1 cut(s)	773
BauI	CACGAG	1 cut(s)	1778
BbrPI	CACGTG	1 cut(s)	1102
BbsI	GAAGAC	1 cut(s)	771
Bbv12I	GWGCWC	1 cut(s)	687
BbvI	GCAGC	3 cut(s)	524, 1297, 1833
BccI	CCATC	7 cut(s)	86, 821, 848, 994, 1295, 1551, 1604
BceAI	ACGGC	5 cut(s)	274, 425, 1298, 1323, 1878
BcgI	CGANNNNNNTGC	6 cut(s)	1074, 1108, 1480, 1514, 1568, 1602
BciT130I	CCWGG	3 cut(s)	578, 718, 1694
BclI	TGATCA	1 cut(s)	879
BcnI	CCSGG	7 cut(s)	464, 523, 680, 712, 739, 1007, 2021
BcoDI	GTCTC	5 cut(s)	709, 838, 1576, 1968, 2151
BfaI	CTAG	1 cut(s)	351
BfoI	RGCGCY	4 cut(s)	385, 643, 777, 2075
BfuAI	ACCTGC	1 cut(s)	616
BglI	GCCNNNNNGGC	1 cut(s)	1610
BglII	AGATCT	1 cut(s)	1053
Bme18I	GGWCC	7 cut(s)	547, 583, 714, 720, 741, 1003, 1393
BmeT110I	CYCGRG	2 cut(s)	190, 1877
BmgT120I	GGNCC	8 cut(s)	547, 583, 714, 720, 741, 1003, 1327, 1393
BmiI	GGNNCC	5 cut(s)	461, 585, 775, 1436, 2159
BoxI	GACNNNNGTC	1 cut(s)	1949
BpiI	GAAGAC	1 cut(s)	771
BpmI	CTGGAG	5 cut(s)	94, 536, 629, 1691, 1727
Bpu14I	TTCGAA	2 cut(s)	896, 1772
BpuMI	CCSGG	7 cut(s)	464, 523, 680, 712, 739, 1007, 2021
Bsa29I	ATCGAT	1 cut(s)	935
BsaAI	YACGTR	4 cut(s)	18, 477, 1102, 1714
BsaBI	GATNNNNATC	1 cut(s)	1058
BsaHI	GRCGYC	3 cut(s)	774, 810, 966
BsaI	GGTCTC	1 cut(s)	838
BsaJI	CCNNGG	9 cut(s)	22, 377, 390, 769, 780, 1305, 1389, 1753, 1878
BsaWI	WCCGGW	1 cut(s)	2011
BsaXI	ACNNNNNCTCC	2 cut(s)	1820, 1850
Bse118I	RCCGGY	3 cut(s)	1424, 1528, 1591
Bse1I	ACTGG	7 cut(s)	49, 111, 206, 1274, 1523, 1744, 1769
Bse3DI	GCAATG	1 cut(s)	639
Bse8I	GATNNNNATC	1 cut(s)	1058
BseBI	CCWGG	3 cut(s)	578, 718, 1694
BseCI	ATCGAT	1 cut(s)	935
BseDI	CCNNGG	9 cut(s)	22, 377, 390, 769, 780, 1305, 1389, 1753, 1878
BseGI	GGATG	6 cut(s)	813, 1109, 1538, 1543, 1675, 2049
BseJI	GATNNNNATC	1 cut(s)	1058
BseMI	GCAATG	1 cut(s)	639
BseMII	CTCAG	5 cut(s)	606, 1013, 1337, 1363, 1880
BseNI	ACTGG	7 cut(s)	49, 111, 206, 1274, 1523, 1744, 1769
BseRI	GAGGAG	3 cut(s)	1229, 1466, 1622
BseXI	GCAGC	3 cut(s)	524, 1297, 1833
BsgI	GTGCAG	1 cut(s)	1572
Bsh1236I	CGCG	9 cut(s)	379, 410, 771, 1128, 1155, 1455, 1457, 1802, 1916
Bsh1285I	CGRYCG	1 cut(s)	2052
BshNI	GGYRCC	1 cut(s)	773
BshVI	ATCGAT	1 cut(s)	935
BsiEI	CGRYCG	1 cut(s)	2052
BsiHKAI	GWGCWC	1 cut(s)	687
BsiHKCI	CYCGRG	2 cut(s)	190, 1877
BsiWI	CGTACG	2 cut(s)	477, 1730
BslFI	GGGAC	3 cut(s)	99, 1403, 1860
BsmAI	GTCTC	5 cut(s)	709, 838, 1576, 1968, 2151
BsmBI	CGTCTC	2 cut(s)	1576, 1968
BsmFI	GGGAC	3 cut(s)	99, 1403, 1860
BsmI	GAATGC	2 cut(s)	878, 908
Bso31I	GGTCTC	1 cut(s)	838
BsoBI	CYCGRG	2 cut(s)	190, 1877
Bsp119I	TTCGAA	2 cut(s)	896, 1772
Bsp1286I	GDGCHC	1 cut(s)	687
Bsp1407I	TGTACA	1 cut(s)	1248
Bsp19I	CCATGG	1 cut(s)	1389
Bsp68I	TCGCGA	1 cut(s)	1155
BspCNI	CTCAG	5 cut(s)	605, 1014, 1336, 1362, 1879
BspDI	ATCGAT	1 cut(s)	935
BspFNI	CGCG	9 cut(s)	379, 410, 771, 1128, 1155, 1455, 1457, 1802, 1916
BspHI	TCATGA	1 cut(s)	450
BspLI	GGNNCC	5 cut(s)	461, 585, 775, 1436, 2159
BspMI	ACCTGC	1 cut(s)	616
BspPI	GGATC	5 cut(s)	658, 1026, 1117, 1429, 1442
BspQI	GCTCTTC	3 cut(s)	1076, 1178, 1199
BspT104I	TTCGAA	2 cut(s)	896, 1772
BspT107I	GGYRCC	1 cut(s)	773
BspTNI	GGTCTC	1 cut(s)	838
BsrBI	CCGCTC	1 cut(s)	973
BsrDI	GCAATG	1 cut(s)	639
BsrFI	RCCGGY	3 cut(s)	1424, 1528, 1591
BsrGI	TGTACA	1 cut(s)	1248
BsrI	ACTGG	7 cut(s)	49, 111, 206, 1274, 1523, 1744, 1769
BssAI	RCCGGY	3 cut(s)	1424, 1528, 1591
BssECI	CCNNGG	9 cut(s)	22, 377, 390, 769, 780, 1305, 1389, 1753, 1878
BssNI	GRCGYC	3 cut(s)	774, 810, 966
BssSI	CACGAG	1 cut(s)	1778
BssT1I	CCWWGG	3 cut(s)	22, 1389, 1753
Bst2BI	CACGAG	1 cut(s)	1778
Bst2UI	CCWGG	3 cut(s)	578, 718, 1694
Bst4CI	ACNGT	3 cut(s)	1048, 2050, 2136
Bst6I	CTCTTC	6 cut(s)	313, 343, 1076, 1178, 1199, 1694
BstACI	GRCGYC	3 cut(s)	774, 810, 966
BstAUI	TGTACA	1 cut(s)	1248
BstBAI	YACGTR	4 cut(s)	18, 477, 1102, 1714
BstBI	TTCGAA	2 cut(s)	896, 1772
BstC8I	GCNNGC	4 cut(s)	1082, 1530, 1606, 1640
BstDEI	CTNAG	5 cut(s)	592, 1022, 1323, 1349, 1866
BstDSI	CCRYGG	6 cut(s)	377, 390, 769, 780, 1305, 1389
BstENI	CCTNNNNNAGG	3 cut(s)	575, 623, 1757
BstF5I	GGATG	6 cut(s)	813, 1109, 1538, 1543, 1675, 2049
BstFNI	CGCG	9 cut(s)	379, 410, 771, 1128, 1155, 1455, 1457, 1802, 1916
BstH2I	RGCGCY	4 cut(s)	385, 643, 777, 2075
BstHHI	GCGC	7 cut(s)	384, 613, 642, 776, 1457, 2038, 2074
BstMAI	GTCTC	5 cut(s)	709, 838, 1576, 1968, 2151
BstMCI	CGRYCG	1 cut(s)	2052
BstMWI	GCNNNNNNNGC	8 cut(s)	809, 1134, 1369, 1601, 1610, 2064, 2078, 2087
BstNI	CCWGG	3 cut(s)	578, 718, 1694
BstNSI	RCATGY	1 cut(s)	1249
BstPAI	GACNNNNGTC	1 cut(s)	1949
BstUI	CGCG	9 cut(s)	379, 410, 771, 1128, 1155, 1455, 1457, 1802, 1916
BstV1I	GCAGC	3 cut(s)	524, 1297, 1833
BstV2I	GAAGAC	1 cut(s)	771
BstX2I	RGATCY	4 cut(s)	663, 1018, 1053, 1434
BstYI	RGATCY	4 cut(s)	663, 1018, 1053, 1434
Bsu15I	ATCGAT	1 cut(s)	935
BsuTUI	ATCGAT	1 cut(s)	935
BtgI	CCRYGG	6 cut(s)	377, 390, 769, 780, 1305, 1389
BtgZI	GCGATG	2 cut(s)	826, 1472
BtsCI	GGATG	6 cut(s)	813, 1109, 1538, 1543, 1675, 2049
BtsI	GCAGTG	1 cut(s)	1251
BtsIMutI	CAGTG	1 cut(s)	1251
BtuMI	TCGCGA	1 cut(s)	1155
BveI	ACCTGC	1 cut(s)	616
Cac8I	GCNNGC	4 cut(s)	1082, 1530, 1606, 1640
CciI	TCATGA	1 cut(s)	450
CfoI	GCGC	7 cut(s)	384, 613, 642, 776, 1457, 2038, 2074
Cfr10I	RCCGGY	3 cut(s)	1424, 1528, 1591
Cfr13I	GGNCC	8 cut(s)	547, 583, 714, 720, 741, 1003, 1327, 1393
Cfr42I	CCGCGG	2 cut(s)	380, 772
ClaI	ATCGAT	1 cut(s)	935
CseI	GACGC	4 cut(s)	416, 818, 895, 955
Csp6I	GTAC	7 cut(s)	15, 478, 1249, 1684, 1731, 1747, 1955
CviAII	CATG	8 cut(s)	451, 562, 622, 673, 877, 1246, 1390, 1609
CviQI	GTAC	7 cut(s)	15, 478, 1249, 1684, 1731, 1747, 1955
DdeI	CTNAG	5 cut(s)	592, 1022, 1323, 1349, 1866
DinI	GGCGCC	1 cut(s)	775
DrdI	GACNNNNNNGTC	1 cut(s)	1568
DseDI	GACNNNNNNGTC	1 cut(s)	1568
EaeI	YGGCCR	4 cut(s)	837, 1271, 1593, 1695
Eam1104I	CTCTTC	6 cut(s)	313, 343, 1076, 1178, 1199, 1694
EarI	CTCTTC	6 cut(s)	313, 343, 1076, 1178, 1199, 1694
EciI	GGCGGA	3 cut(s)	1223, 1515, 1591
Eco130I	CCWWGG	3 cut(s)	22, 1389, 1753
Eco147I	AGGCCT	1 cut(s)	355
Eco31I	GGTCTC	1 cut(s)	838
Eco47I	GGWCC	7 cut(s)	547, 583, 714, 720, 741, 1003, 1393
Eco57I	CTGAAG	1 cut(s)	2115
Eco72I	CACGTG	1 cut(s)	1102
Eco88I	CYCGRG	2 cut(s)	190, 1877
EcoNI	CCTNNNNNAGG	3 cut(s)	575, 623, 1757
EcoO109I	RGGNCCY	3 cut(s)	583, 720, 1327
EcoRII	CCWGG	3 cut(s)	576, 716, 1692
EcoT14I	CCWWGG	3 cut(s)	22, 1389, 1753
EcoT22I	ATGCAT	1 cut(s)	878
EgeI	GGCGCC	1 cut(s)	775
EheI	GGCGCC	1 cut(s)	775
ErhI	CCWWGG	3 cut(s)	22, 1389, 1753
Esp3I	CGTCTC	2 cut(s)	1576, 1968
FaeI	CATG	8 cut(s)	454, 565, 625, 676, 880, 1249, 1393, 1612
FaqI	GGGAC	3 cut(s)	99, 1403, 1860
FatI	CATG	8 cut(s)	450, 561, 621, 672, 876, 1245, 1389, 1608
FauI	CCCGC	6 cut(s)	384, 1191, 1424, 1613, 2051, 2060
FbaI	TGATCA	1 cut(s)	879
FokI	GGATG	6 cut(s)	800, 1096, 1525, 1530, 1682, 2056
FspBI	CTAG	1 cut(s)	351
GlaI	GCGC	7 cut(s)	383, 612, 641, 775, 1456, 2037, 2073
GsuI	CTGGAG	5 cut(s)	94, 536, 629, 1691, 1727
HaeII	RGCGCY	4 cut(s)	385, 643, 777, 2075
HgaI	GACGC	4 cut(s)	416, 818, 895, 955
HhaI	GCGC	7 cut(s)	384, 613, 642, 776, 1457, 2038, 2074
Hin1I	GRCGYC	3 cut(s)	774, 810, 966
Hin1II	CATG	8 cut(s)	454, 565, 625, 676, 880, 1249, 1393, 1612
Hin6I	GCGC	7 cut(s)	382, 611, 640, 774, 1455, 2036, 2072
HinP1I	GCGC	7 cut(s)	382, 611, 640, 774, 1455, 2036, 2072
HphI	GGTGA	4 cut(s)	67, 1060, 1213, 1471
Hpy166II	GTNNAC	3 cut(s)	910, 1251, 2017
Hpy8I	GTNNAC	3 cut(s)	910, 1251, 2017
HpyAV	CCTTC	4 cut(s)	327, 357, 366, 892
HpyCH4III	ACNGT	3 cut(s)	1048, 2050, 2136
HpyCH4IV	ACGT	7 cut(s)	17, 476, 792, 1101, 1494, 1713, 1733
HpyCH4V	TGCA	5 cut(s)	565, 625, 876, 1589, 1638
HpyF10VI	GCNNNNNNNGC	8 cut(s)	809, 1134, 1369, 1601, 1610, 2064, 2078, 2087
HpyF3I	CTNAG	5 cut(s)	592, 1022, 1323, 1349, 1866
HpySE526I	ACGT	7 cut(s)	17, 476, 792, 1101, 1494, 1713, 1733
Hsp92I	GRCGYC	3 cut(s)	774, 810, 966
Hsp92II	CATG	8 cut(s)	454, 565, 625, 676, 880, 1249, 1393, 1612
HspAI	GCGC	7 cut(s)	382, 611, 640, 774, 1455, 2036, 2072
KasI	GGCGCC	1 cut(s)	773
KroI	GCCGGC	1 cut(s)	1528
KroNI	GCCGGC	1 cut(s)	1530
Ksp22I	TGATCA	1 cut(s)	879
KspI	CCGCGG	2 cut(s)	380, 772
LguI	GCTCTTC	3 cut(s)	1076, 1178, 1199
LmnI	GCTCC	5 cut(s)	264, 682, 1479, 1588, 2157
Lsp1109I	GCAGC	3 cut(s)	524, 1297, 1833
MaeI	CTAG	1 cut(s)	351
MaeII	ACGT	7 cut(s)	17, 476, 792, 1101, 1494, 1713, 1733
MaeIII	GTNAC	2 cut(s)	487, 541
MbiI	CCGCTC	1 cut(s)	973
MflI	RGATCY	4 cut(s)	663, 1018, 1053, 1434
MhlI	GDGCHC	1 cut(s)	687
MlsI	TGGCCA	1 cut(s)	1697
MluCI	AATT	8 cut(s)	183, 236, 797, 860, 1462, 1817, 1909, 2085
MluNI	TGGCCA	1 cut(s)	1697
Mly113I	GGCGCC	1 cut(s)	774
MlyI	GAGTC	6 cut(s)	13, 35, 1139, 1394, 1397, 1441
MmeI	TCCRAC	4 cut(s)	395, 1599, 2083, 2142
Mox20I	TGGCCA	1 cut(s)	1697
Mph1103I	ATGCAT	1 cut(s)	878
MreI	CGCCGGCG	1 cut(s)	1528
MroNI	GCCGGC	1 cut(s)	1528
MroXI	GAANNNNTTC	1 cut(s)	653
MscI	TGGCCA	1 cut(s)	1697
MseI	TTAA	3 cut(s)	414, 863, 1983
MslI	CAYNNNNRTG	2 cut(s)	626, 845
Msp20I	TGGCCA	1 cut(s)	1697
MspA1I	CMGCKG	3 cut(s)	379, 537, 771
Mva1269I	GAATGC	2 cut(s)	878, 908
MvaI	CCWGG	3 cut(s)	578, 718, 1694
MvnI	CGCG	9 cut(s)	379, 410, 771, 1128, 1155, 1455, 1457, 1802, 1916
MwoI	GCNNNNNNNGC	8 cut(s)	809, 1134, 1369, 1601, 1610, 2064, 2078, 2087
NaeI	GCCGGC	1 cut(s)	1530
NarI	GGCGCC	1 cut(s)	774
NciI	CCSGG	7 cut(s)	464, 523, 680, 712, 739, 1007, 2021
NcoI	CCATGG	1 cut(s)	1389
NgoMIV	GCCGGC	1 cut(s)	1528
NlaIII	CATG	8 cut(s)	454, 565, 625, 676, 880, 1249, 1393, 1612
NlaIV	GGNNCC	5 cut(s)	461, 585, 775, 1436, 2159
NmeAIII	GCCGAG	2 cut(s)	815, 942
NmuCI	GTSAC	2 cut(s)	487, 541
NruI	TCGCGA	1 cut(s)	1155
NsiI	ATGCAT	1 cut(s)	878
NspI	RCATGY	1 cut(s)	1249
NspV	TTCGAA	2 cut(s)	896, 1772
PaeR7I	CTCGAG	1 cut(s)	190
PagI	TCATGA	1 cut(s)	450
PaqCI	CACCTGC	1 cut(s)	616
PceI	AGGCCT	1 cut(s)	355
PciI	ACATGT	1 cut(s)	1245
PciSI	GCTCTTC	3 cut(s)	1076, 1178, 1199
PcsI	WCGNNNNNNNCGW	5 cut(s)	816, 849, 893, 1566, 1920
PctI	GAATGC	2 cut(s)	878, 908
PdiI	GCCGGC	1 cut(s)	1530
PdmI	GAANNNNTTC	1 cut(s)	653
PfeI	GAWTC	7 cut(s)	698, 951, 1086, 1283, 1301, 1355, 1738
Pfl23II	CGTACG	2 cut(s)	477, 1730
PflFI	GACNNNGTC	1 cut(s)	739
PfoI	TCCNGGA	1 cut(s)	576
PleI	GAGTC	6 cut(s)	12, 35, 1138, 1393, 1397, 1441
PluTI	GGCGCC	1 cut(s)	777
PmaCI	CACGTG	1 cut(s)	1102
PmlI	CACGTG	1 cut(s)	1102
PpsI	GAGTC	6 cut(s)	12, 35, 1138, 1393, 1397, 1441
Ppu21I	YACGTR	4 cut(s)	18, 477, 1102, 1714
PpuMI	RGGWCCY	2 cut(s)	583, 720
PscI	ACATGT	1 cut(s)	1245
PshAI	GACNNNNGTC	1 cut(s)	1949
PshBI	ATTAAT	1 cut(s)	863
Psp5II	RGGWCCY	2 cut(s)	583, 720
Psp6I	CCWGG	3 cut(s)	576, 716, 1692
PspCI	CACGTG	1 cut(s)	1102
PspGI	CCWGG	3 cut(s)	576, 716, 1692
PspLI	CGTACG	2 cut(s)	477, 1730
PspN4I	GGNNCC	5 cut(s)	461, 585, 775, 1436, 2159
PspPI	GGNCC	8 cut(s)	547, 583, 714, 720, 741, 1003, 1327, 1393
PspPPI	RGGWCCY	2 cut(s)	583, 720
PsuI	RGATCY	4 cut(s)	663, 1018, 1053, 1434
PsyI	GACNNNGTC	1 cut(s)	739
RruI	TCGCGA	1 cut(s)	1155
RsaI	GTAC	7 cut(s)	16, 479, 1250, 1685, 1732, 1748, 1956
RsaNI	GTAC	7 cut(s)	15, 478, 1249, 1684, 1731, 1747, 1955
RseI	CAYNNNNRTG	2 cut(s)	626, 845
SacII	CCGCGG	2 cut(s)	380, 772
SapI	GCTCTTC	3 cut(s)	1076, 1178, 1199
SaqAI	TTAA	3 cut(s)	414, 863, 1983
Sau96I	GGNCC	8 cut(s)	547, 583, 714, 720, 741, 1003, 1327, 1393
SchI	GAGTC	6 cut(s)	13, 35, 1139, 1394, 1397, 1441
SduI	GDGCHC	1 cut(s)	687
SfoI	GGCGCC	1 cut(s)	775
Sfr274I	CTCGAG	1 cut(s)	190
Sfr303I	CCGCGG	2 cut(s)	380, 772
SfuI	TTCGAA	2 cut(s)	896, 1772
SgrAI	CRCCGGYG	2 cut(s)	1424, 1528
SgrBI	CCGCGG	2 cut(s)	380, 772
SinI	GGWCC	7 cut(s)	547, 583, 714, 720, 741, 1003, 1393
SlaI	CTCGAG	1 cut(s)	190
SmiMI	CAYNNNNRTG	2 cut(s)	626, 845
SmlI	CTYRAG	1 cut(s)	190
SmoI	CTYRAG	1 cut(s)	190
Sse9I	AATT	8 cut(s)	183, 236, 797, 860, 1462, 1817, 1909, 2085
SseBI	AGGCCT	1 cut(s)	355
SspDI	GGCGCC	1 cut(s)	773
SspMI	CTAG	1 cut(s)	351
StuI	AGGCCT	1 cut(s)	355
StyI	CCWWGG	3 cut(s)	22, 1389, 1753
TaaI	ACNGT	3 cut(s)	1048, 2050, 2136
TaiI	ACGT	7 cut(s)	20, 479, 795, 1104, 1497, 1716, 1736
TasI	AATT	8 cut(s)	183, 236, 797, 860, 1462, 1817, 1909, 2085
TatI	WGTACW	1 cut(s)	1248
TauI	GCSGC	8 cut(s)	302, 382, 440, 774, 1163, 1431, 1795, 1805
TfiI	GAWTC	7 cut(s)	698, 951, 1086, 1283, 1301, 1355, 1738
Tru1I	TTAA	3 cut(s)	414, 863, 1983
Tru9I	TTAA	3 cut(s)	414, 863, 1983
TscAI	CASTG	1 cut(s)	1258
TseFI	GTSAC	2 cut(s)	487, 541
TseI	GCWGC	3 cut(s)	537, 1310, 1846
Tsp45I	GTSAC	2 cut(s)	487, 541
TspDTI	ATGAA	6 cut(s)	354, 467, 650, 689, 863, 1475
TspGWI	ACGGA	8 cut(s)	349, 419, 560, 797, 1157, 1307, 1405, 1718
TspRI	CASTG	1 cut(s)	1258
Tth111I	GACNNNGTC	1 cut(s)	739
VpaK11BI	GGWCC	7 cut(s)	547, 583, 714, 720, 741, 1003, 1393
VspI	ATTAAT	1 cut(s)	863
XagI	CCTNNNNNAGG	3 cut(s)	575, 623, 1757
XapI	RAATTY	2 cut(s)	183, 797
XceI	RCATGY	1 cut(s)	1249
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1751
XhoI	CTCGAG	1 cut(s)	190
XmnI	GAANNNNTTC	1 cut(s)	653
XspI	CTAG	1 cut(s)	351
Zsp2I	ATGCAT	1 cut(s)	878

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.