pycom12g20060

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0022421
Physical Location & Seq
Reverse (-)
21711878 .. 21712621
744 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 744 bp
ATGGCTCAAGAGCTGAAAAAGTTTTGGAATCAATCCATATTGCAGCAATTACAAATAATGTTCCTGGGTACGTGTTTGTCGTGGTCCCTGTTGGTGTCCTGTCCATTGAGTCACCCTTCTCATTTGAGACTATCGTCGGGGTGGAAGGATTGGAGATTGACAAAGGATATATCTTCCCTCAATTTGCTACAAACCCTGAGAAATTGTTTGTTGAATTCGAGAATGCTGGAGTTCCGATCACTGCAATACCAGAAGATTTCAAGGATGTTTAAAGTAGTGGAAGACTGCCGAGCAGCTGGAATTCTTGTTATGGTAATCACGGGAGATAACCAGAACATTGCAGAAGCAATATGCCGTGAAATAGGCGTGTTTAGTACTGATGAAGACGTACATTCAAGAAGCCTTACAAGAAAAGAGTTTATGAGTACTATACGCGATCCAAAAGCCCATCTGAGACAAGATGATGGCCTTCTGTTTTCAAGTGTCGAACCAAAGCACATTGTGAGGTTGCTTAAAGAGGATGGTGAGGTTGCTGCTATAACTGTTGATGGAATGAATGGTGCATCTACTTTGAAGCTTGCTGATATCGGGATTGTAATGGCCATTTCTGGAACTGAGGTGCTTCTAGAGCTGAAGGAGAGTGCTGCTGCGTTCAAACAAGTCCCCACAATTTCTTTTTTAAGCCTTGAGGACAAGGCTAATTTTCAAGGGGGAAGTATTGTTATGTACCCAATTTGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

248

Amino Acids

27.68

Weight (kDa)

6.95

Isoelectric Point (pI)

43.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022421)

Species Orthologous Gene IDs
malus_domestica MD17G1211600.v1.1
pyrus_communis pycom12g20060
rosa_multiflora Rmu_sc0034329.1_g000001 Rmu_sc0034875.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 435
AclWI GGATC 1 cut(s) 431
AcoI YGGCCR 1 cut(s) 600
AcsI RAATTY 2 cut(s) 214, 300
AcuI CTGAAG 1 cut(s) 653
AdeI CACNNNGTG 1 cut(s) 502
AfaI GTAC 5 cut(s) 70, 376, 390, 427, 728
AflIII ACRYGT 1 cut(s) 71
AgsI TTSAA 7 cut(s) 214, 261, 396, 480, 574, 655, 707
AjnI CCWGG 1 cut(s) 63
AluBI AGCT 4 cut(s) 13, 296, 577, 631
AluI AGCT 4 cut(s) 13, 296, 577, 631
Alw26I GTCTC 2 cut(s) 121, 448
AlwI GGATC 1 cut(s) 431
AoxI GGCC 2 cut(s) 466, 600
ApeKI GCWGC 5 cut(s) 43, 293, 533, 644, 647
ApoI RAATTY 2 cut(s) 214, 300
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 2 cut(s) 104, 536
AvaII GGWCC 1 cut(s) 84
BalI TGGCCA 1 cut(s) 602
BbsI GAAGAC 2 cut(s) 288, 390
BbvI GCAGC 5 cut(s) 55, 305, 520, 631, 634
BccI CCATC 4 cut(s) 456, 458, 515, 542
BceAI ACGGC 1 cut(s) 339
BciT130I CCWGG 1 cut(s) 65
BcoDI GTCTC 2 cut(s) 121, 448
BfaI CTAG 1 cut(s) 626
BisI GCNGC 5 cut(s) 44, 294, 534, 645, 648
BlsI GCNGC 5 cut(s) 45, 295, 535, 646, 649
BmcAI AGTACT 2 cut(s) 376, 427
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 84
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 86
BmrFI CCNGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 572
BoxI GACNNNNGTC 1 cut(s) 133
BpiI GAAGAC 2 cut(s) 288, 390
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 707
BsaAI YACGTR 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 64
Bse3DI GCAATG 1 cut(s) 336
BseBI CCWGG 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 64
BseGI GGATG 2 cut(s) 270, 526
BseMI GCAATG 1 cut(s) 336
BseMII CTCAG 3 cut(s) 188, 443, 606
BseXI GCAGC 5 cut(s) 55, 305, 520, 631, 634
Bsh1236I CGCG 1 cut(s) 435
BshFI GGCC 2 cut(s) 468, 602
BslFI GGGAC 2 cut(s) 70, 647
BsmAI GTCTC 2 cut(s) 121, 448
BsmFI GGGAC 2 cut(s) 70, 647
BsmI GAATGC 1 cut(s) 228
BsnI GGCC 2 cut(s) 468, 602
Bsp143I GATC 2 cut(s) 236, 436
BspANI GGCC 2 cut(s) 468, 602
BspCNI CTCAG 3 cut(s) 189, 444, 607
BspFNI CGCG 1 cut(s) 435
BspLI GGNNCC 1 cut(s) 86
BspPI GGATC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 336
BssECI CCNNGG 1 cut(s) 64
BssMI GATC 2 cut(s) 236, 436
Bst2UI CCWGG 1 cut(s) 65
Bst4CI ACNGT 1 cut(s) 544
BstBAI YACGTR 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 579
BstDEI CTNAG 3 cut(s) 197, 452, 615
BstF5I GGATG 2 cut(s) 270, 526
BstFNI CGCG 1 cut(s) 435
BstKTI GATC 2 cut(s) 239, 439
BstMAI GTCTC 2 cut(s) 121, 448
BstMBI GATC 2 cut(s) 236, 436
BstMWI GCNNNNNNNGC 1 cut(s) 628
BstNI CCWGG 1 cut(s) 65
BstPAI GACNNNNGTC 1 cut(s) 133
BstSCI CCNGG 1 cut(s) 63
BstUI CGCG 1 cut(s) 435
BstV1I GCAGC 5 cut(s) 55, 305, 520, 631, 634
BstV2I GAAGAC 2 cut(s) 288, 390
BsuRI GGCC 2 cut(s) 468, 602
BtsCI GGATG 2 cut(s) 270, 526
BtsI GCAGTG 1 cut(s) 239
BtsIMutI CAGTG 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 579
Cfr13I GGNCC 1 cut(s) 84
Csp6I GTAC 5 cut(s) 69, 375, 389, 426, 727
CviQI GTAC 5 cut(s) 69, 375, 389, 426, 727
DdeI CTNAG 3 cut(s) 197, 452, 615
DpnI GATC 2 cut(s) 238, 438
DpnII GATC 2 cut(s) 236, 436
DraI TTTAAA 1 cut(s) 271
DraIII CACNNNGTG 1 cut(s) 502
EaeI YGGCCR 1 cut(s) 600
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 1 cut(s) 84
Eco57I CTGAAG 1 cut(s) 653
EcoRI GAATTC 2 cut(s) 214, 300
EcoRII CCWGG 1 cut(s) 63
EcoRV GATATC 1 cut(s) 586
FaiI YATR 8 cut(s) 38, 170, 311, 352, 422, 431, 539, 725
FaqI GGGAC 2 cut(s) 70, 647
Fnu4HI GCNGC 5 cut(s) 44, 294, 534, 645, 648
FokI GGATG 2 cut(s) 277, 533
Fsp4HI GCNGC 5 cut(s) 44, 294, 534, 645, 648
FspBI CTAG 1 cut(s) 626
GluI GCNGC 5 cut(s) 44, 294, 534, 645, 648
GsuI CTGGAG 1 cut(s) 248
HaeIII GGCC 2 cut(s) 468, 602
HindIII AAGCTT 1 cut(s) 575
HinfI GANTC 2 cut(s) 28, 109
HphI GGTGA 2 cut(s) 104, 536
Hpy188I TCNGA 2 cut(s) 236, 453
Hpy188III TCNNGA 6 cut(s) 8, 219, 396, 589, 609, 626
Hpy99I CGWCG 1 cut(s) 139
HpyAV CCTTC 4 cut(s) 126, 139, 479, 628
HpyCH4III ACNGT 1 cut(s) 544
HpyCH4IV ACGT 2 cut(s) 71, 387
HpyCH4V TGCA 4 cut(s) 43, 244, 341, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 628
HpyF3I CTNAG 3 cut(s) 197, 452, 615
HpySE526I ACGT 2 cut(s) 71, 387
Kzo9I GATC 2 cut(s) 236, 436
Lsp1109I GCAGC 5 cut(s) 55, 305, 520, 631, 634
LweI GCATC 1 cut(s) 572
MaeI CTAG 1 cut(s) 626
MaeII ACGT 2 cut(s) 71, 387
MaeIII GTNAC 1 cut(s) 110
MalI GATC 2 cut(s) 238, 438
MboI GATC 2 cut(s) 236, 436
MboII GAAGA 4 cut(s) 165, 265, 293, 395
MlsI TGGCCA 1 cut(s) 602
MluCI AATT 9 cut(s) 47, 181, 202, 214, 300, 669, 700, 732, 739
MluNI TGGCCA 1 cut(s) 602
MlyI GAGTC 1 cut(s) 118
MnlI CCTC 6 cut(s) 188, 498, 511, 520, 610, 682
Mox20I TGGCCA 1 cut(s) 602
MscI TGGCCA 1 cut(s) 602
MseI TTAA 3 cut(s) 270, 513, 680
Msp20I TGGCCA 1 cut(s) 602
MspA1I CMGCKG 1 cut(s) 296
MspR9I CCNGG 1 cut(s) 65
Mva1269I GAATGC 1 cut(s) 228
MvaI CCWGG 1 cut(s) 65
MvnI CGCG 1 cut(s) 435
MwoI GCNNNNNNNGC 1 cut(s) 628
NdeII GATC 2 cut(s) 236, 436
NlaIV GGNNCC 1 cut(s) 86
NmeAIII GCCGAG 1 cut(s) 314
NmuCI GTSAC 1 cut(s) 110
PcsI WCGNNNNNNNCGW 1 cut(s) 77
PctI GAATGC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 28
PkrI GCNGC 5 cut(s) 45, 295, 535, 646, 649
PleI GAGTC 1 cut(s) 117
PpsI GAGTC 1 cut(s) 117
Ppu21I YACGTR 1 cut(s) 72
PshAI GACNNNNGTC 1 cut(s) 133
Psp6I CCWGG 1 cut(s) 63
PspGI CCWGG 1 cut(s) 63
PspN4I GGNNCC 1 cut(s) 86
PspPI GGNCC 1 cut(s) 84
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 5 cut(s) 70, 376, 390, 427, 728
RsaNI GTAC 5 cut(s) 69, 375, 389, 426, 727
SaqAI TTAA 3 cut(s) 270, 513, 680
SatI GCNGC 5 cut(s) 44, 294, 534, 645, 648
Sau3AI GATC 2 cut(s) 236, 436
Sau96I GGNCC 1 cut(s) 84
ScaI AGTACT 2 cut(s) 376, 427
SchI GAGTC 1 cut(s) 118
ScrFI CCNGG 1 cut(s) 65
SetI ASST 9 cut(s) 15, 74, 298, 390, 509, 531, 579, 621, 633
SfaNI GCATC 1 cut(s) 572
SinI GGWCC 1 cut(s) 84
SmlI CTYRAG 2 cut(s) 6, 686
SmoI CTYRAG 2 cut(s) 6, 686
Sse9I AATT 9 cut(s) 47, 181, 202, 214, 300, 669, 700, 732, 739
SspMI CTAG 1 cut(s) 626
StyD4I CCNGG 1 cut(s) 63
TaaI ACNGT 1 cut(s) 544
TaiI ACGT 2 cut(s) 74, 390
TaqI TCGA 2 cut(s) 218, 486
TasI AATT 9 cut(s) 47, 181, 202, 214, 300, 669, 700, 732, 739
TatI WGTACW 2 cut(s) 374, 425
TfiI GAWTC 1 cut(s) 28
Tru1I TTAA 3 cut(s) 270, 513, 680
Tru9I TTAA 3 cut(s) 270, 513, 680
TscAI CASTG 1 cut(s) 246
TseFI GTSAC 1 cut(s) 110
TseI GCWGC 5 cut(s) 43, 293, 533, 644, 647
Tsp45I GTSAC 1 cut(s) 110
TspDTI ATGAA 2 cut(s) 396, 569
TspRI CASTG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 84
XapI RAATTY 2 cut(s) 214, 300
XbaI TCTAGA 1 cut(s) 625
XspI CTAG 1 cut(s) 626
ZrmI AGTACT 2 cut(s) 376, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.