pycom12g20990

Belongs to the glycosyl hydrolase 17 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0020148
Physical Location & Seq
Forward (+)
22282366 .. 22282773
408 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 408 bp
ATGGTGGTTGTACTATACGCATCACTGGAGAAAGCAGCGGCGCCTCGTGTCCAGATGGTGGTGTCTGAGACCGGTTGGCCCTCCGACGGGAATGGAAACGTGACAGCACCCGGCAATGCGCAAACATACAACTCGAATTTAATAAAGCATGTGTTGTCGTCCAGTGGAACTCCAAGGCGGCCGGGAAGCTCCATCGACACGTACATTTTTTCCATGTTTAATGAGAACACGAAGCCCGGCGATGCAGTGGAGCAACACTGGGGTCTGTTTTATCCGAACAAGAGTTCCGTTTATCCAATCAACTTCGGGAGCAAAAGATCGAGAGACATAAAAATAGCATACTATTTTGTTGCTGCTTCTGTTATTATACTACTTGTAATAGGTAATGGGAAGATGCAATTTGATTAG

Protein Analysis

136

Amino Acids

14.77

Weight (kDa)

9.22

Isoelectric Point (pI)

33.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_17 PF00332 4 - 102 4.7e-34 Glycosyl hydrolases family 17
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020148)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57260 AT3G57260
malus_domestica MD12G1224300.v1.1 MD14G1078800.v1.1
pyrus_communis pycom12g20990
rosa_rugosa Rorug06G0414000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 40
AccB7I CCANNNNNTGG 1 cut(s) 58
AciI CCGC 2 cut(s) 38, 178
AcoI YGGCCR 1 cut(s) 179
AcsI RAATTY 1 cut(s) 136
AcyI GRCGYC 1 cut(s) 41
AfaI GTAC 2 cut(s) 12, 203
AfiI CCNNNNNNNGG 3 cut(s) 58, 86, 87
AflIII ACRYGT 1 cut(s) 198
AgeI ACCGGT 1 cut(s) 71
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 1 cut(s) 189
AluI AGCT 1 cut(s) 189
Alw26I GTCTC 2 cut(s) 62, 318
AoxI GGCC 2 cut(s) 77, 179
ApeKI GCWGC 2 cut(s) 35, 353
ApoI RAATTY 1 cut(s) 136
AsiGI ACCGGT 1 cut(s) 71
AspLEI GCGC 2 cut(s) 43, 121
AspS9I GGNCC 1 cut(s) 78
AsuC2I CCSGG 3 cut(s) 111, 183, 237
BanI GGYRCC 1 cut(s) 40
BauI CACGAG 1 cut(s) 45
BbvI GCAGC 2 cut(s) 47, 340
BccI CCATC 2 cut(s) 49, 200
BcnI CCSGG 3 cut(s) 111, 183, 237
BcoDI GTCTC 2 cut(s) 62, 318
BfoI RGCGCY 1 cut(s) 44
BisI GCNGC 4 cut(s) 36, 39, 179, 354
BlsI GCNGC 4 cut(s) 37, 40, 180, 355
Bme1390I CCNGG 3 cut(s) 111, 183, 237
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 1 cut(s) 42
BmrFI CCNGG 3 cut(s) 111, 183, 237
BmrI ACTGGG 1 cut(s) 268
BmsI GCATC 3 cut(s) 29, 232, 384
BmuI ACTGGG 1 cut(s) 268
BpmI CTGGAG 1 cut(s) 47
BpuMI CCSGG 3 cut(s) 111, 183, 237
BsaAI YACGTR 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 41
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 173
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 86, 87
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 3 cut(s) 30, 162, 263
Bse3DI GCAATG 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 173
BseLI CCNNNNNNNGG 3 cut(s) 58, 86, 87
BseMI GCAATG 1 cut(s) 121
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 3 cut(s) 30, 162, 263
BseX3I CGGCCG 1 cut(s) 179
BseXI GCAGC 2 cut(s) 47, 340
Bsh1285I CGRYCG 1 cut(s) 182
BshFI GGCC 2 cut(s) 79, 181
BshNI GGYRCC 1 cut(s) 40
BshTI ACCGGT 1 cut(s) 71
BsiEI CGRYCG 1 cut(s) 182
BsiSI CCGG 4 cut(s) 72, 111, 182, 237
BslI CCNNNNNNNGG 3 cut(s) 58, 86, 87
BsmAI GTCTC 2 cut(s) 62, 318
BsnI GGCC 2 cut(s) 79, 181
Bso31I GGTCTC 1 cut(s) 62
Bsp143I GATC 1 cut(s) 317
BspACI CCGC 2 cut(s) 38, 178
BspANI GGCC 2 cut(s) 79, 181
BspCNI CTCAG 1 cut(s) 58
BspLI GGNNCC 1 cut(s) 42
BspT107I GGYRCC 1 cut(s) 40
BspTNI GGTCTC 1 cut(s) 62
BsrDI GCAATG 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 3 cut(s) 30, 162, 263
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 1 cut(s) 317
BssNI GRCGYC 1 cut(s) 41
BssSI CACGAG 1 cut(s) 45
BssT1I CCWWGG 1 cut(s) 173
Bst2BI CACGAG 1 cut(s) 45
BstACI GRCGYC 1 cut(s) 41
BstBAI YACGTR 1 cut(s) 201
BstDEI CTNAG 1 cut(s) 66
BstH2I RGCGCY 1 cut(s) 44
BstHHI GCGC 2 cut(s) 43, 121
BstKTI GATC 1 cut(s) 320
BstMAI GTCTC 2 cut(s) 62, 318
BstMBI GATC 1 cut(s) 317
BstMCI CGRYCG 1 cut(s) 182
BstNSI RCATGY 1 cut(s) 152
BstSCI CCNGG 3 cut(s) 109, 181, 235
BstV1I GCAGC 2 cut(s) 47, 340
BstZI CGGCCG 1 cut(s) 179
BsuRI GGCC 2 cut(s) 79, 181
BtgZI GCGATG 1 cut(s) 255
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 4 cut(s) 23, 169, 252, 256
CfoI GCGC 2 cut(s) 43, 121
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 78
Csp6I GTAC 2 cut(s) 11, 202
CspAI ACCGGT 1 cut(s) 71
CviAII CATG 2 cut(s) 149, 214
CviJI RGCY 4 cut(s) 79, 181, 189, 235
CviKI_1 RGCY 4 cut(s) 79, 181, 189, 235
CviQI GTAC 2 cut(s) 11, 202
DdeI CTNAG 1 cut(s) 66
DinI GGCGCC 1 cut(s) 42
DpnI GATC 1 cut(s) 319
DpnII GATC 1 cut(s) 317
EaeI YGGCCR 1 cut(s) 179
EagI CGGCCG 1 cut(s) 179
EclXI CGGCCG 1 cut(s) 179
Eco130I CCWWGG 1 cut(s) 173
Eco31I GGTCTC 1 cut(s) 62
Eco52I CGGCCG 1 cut(s) 179
EcoT14I CCWWGG 1 cut(s) 173
EgeI GGCGCC 1 cut(s) 42
EheI GGCGCC 1 cut(s) 42
ErhI CCWWGG 1 cut(s) 173
FaeI CATG 2 cut(s) 152, 217
FaiI YATR 7 cut(s) 16, 127, 150, 215, 329, 340, 368
FatI CATG 2 cut(s) 148, 213
Fnu4HI GCNGC 4 cut(s) 36, 39, 179, 354
Fsp4HI GCNGC 4 cut(s) 36, 39, 179, 354
FspI TGCGCA 1 cut(s) 120
GlaI GCGC 2 cut(s) 42, 120
GluI GCNGC 4 cut(s) 36, 39, 179, 354
GsuI CTGGAG 1 cut(s) 47
HaeII RGCGCY 1 cut(s) 44
HaeIII GGCC 2 cut(s) 79, 181
HapII CCGG 4 cut(s) 72, 111, 182, 237
HhaI GCGC 2 cut(s) 43, 121
Hin1I GRCGYC 1 cut(s) 41
Hin1II CATG 2 cut(s) 152, 217
Hin6I GCGC 2 cut(s) 41, 119
HinP1I GCGC 2 cut(s) 41, 119
HpaII CCGG 4 cut(s) 72, 111, 182, 237
Hpy188I TCNGA 3 cut(s) 67, 85, 276
Hpy188III TCNNGA 3 cut(s) 52, 307, 321
Hpy99I CGWCG 1 cut(s) 89
HpyCH4IV ACGT 2 cut(s) 99, 200
HpyCH4V TGCA 2 cut(s) 245, 397
HpyF3I CTNAG 1 cut(s) 66
HpySE526I ACGT 2 cut(s) 99, 200
Hsp92I GRCGYC 1 cut(s) 41
Hsp92II CATG 2 cut(s) 152, 217
HspAI GCGC 2 cut(s) 41, 119
KasI GGCGCC 1 cut(s) 40
Kzo9I GATC 1 cut(s) 317
LmnI GCTCC 3 cut(s) 194, 250, 309
LpnPI CCDG 8 cut(s) 11, 65, 85, 124, 175, 195, 244, 250
Lsp1109I GCAGC 2 cut(s) 47, 340
LweI GCATC 3 cut(s) 29, 232, 384
MaeII ACGT 2 cut(s) 99, 200
MaeIII GTNAC 1 cut(s) 100
MalI GATC 1 cut(s) 319
MboI GATC 1 cut(s) 317
MboII GAAGA 1 cut(s) 403
MluCI AATT 2 cut(s) 136, 398
Mly113I GGCGCC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 108
MnlI CCTC 2 cut(s) 54, 91
MseI TTAA 2 cut(s) 140, 219
MspA1I CMGCKG 1 cut(s) 38
MspI CCGG 4 cut(s) 72, 111, 182, 237
MspR9I CCNGG 3 cut(s) 111, 183, 237
NarI GGCGCC 1 cut(s) 41
NciI CCSGG 3 cut(s) 111, 183, 237
NdeII GATC 1 cut(s) 317
NlaIII CATG 2 cut(s) 152, 217
NlaIV GGNNCC 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 100
NsbI TGCGCA 1 cut(s) 120
NspI RCATGY 1 cut(s) 152
PflMI CCANNNNNTGG 1 cut(s) 58
PinAI ACCGGT 1 cut(s) 71
PkrI GCNGC 4 cut(s) 37, 40, 180, 355
PluTI GGCGCC 1 cut(s) 44
Ppu21I YACGTR 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 42
PspPI GGNCC 1 cut(s) 78
RsaI GTAC 2 cut(s) 12, 203
RsaNI GTAC 2 cut(s) 11, 202
SaqAI TTAA 2 cut(s) 140, 219
SatI GCNGC 4 cut(s) 36, 39, 179, 354
Sau3AI GATC 1 cut(s) 317
Sau96I GGNCC 1 cut(s) 78
ScrFI CCNGG 3 cut(s) 111, 183, 237
SetI ASST 4 cut(s) 102, 191, 203, 385
SfaNI GCATC 3 cut(s) 29, 232, 384
SfoI GGCGCC 1 cut(s) 42
Sse9I AATT 2 cut(s) 136, 398
SsiI CCGC 2 cut(s) 38, 178
SspDI GGCGCC 1 cut(s) 40
StyD4I CCNGG 3 cut(s) 109, 181, 235
StyI CCWWGG 1 cut(s) 173
TaiI ACGT 2 cut(s) 102, 203
TaqI TCGA 3 cut(s) 134, 195, 320
TasI AATT 2 cut(s) 136, 398
TatI WGTACW 1 cut(s) 10
TauI GCSGC 2 cut(s) 41, 181
Tru1I TTAA 2 cut(s) 140, 219
Tru9I TTAA 2 cut(s) 140, 219
TscAI CASTG 4 cut(s) 30, 169, 252, 263
TseFI GTSAC 1 cut(s) 100
TseI GCWGC 2 cut(s) 35, 353
Tsp45I GTSAC 1 cut(s) 100
TspGWI ACGGA 1 cut(s) 277
TspRI CASTG 4 cut(s) 30, 169, 252, 263
Van91I CCANNNNNTGG 1 cut(s) 58
XapI RAATTY 1 cut(s) 136
XceI RCATGY 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.