pycom13g16260

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0012374
Physical Location & Seq
Forward (+)
11976951 .. 11980584
3634 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16260.2

Sequence Viewer

Length: 1533 bp
ATGTCTCTCTCCCTCCTCCCCCTCCTCCTCCTGCTCTCCCTCCTCCCCCTCTCCCTCTCCCTCTCCCTCCCCAGAGGTACTCTAATCAACTGCGGCGCCGCCGTCAAATCCGAAATCGACGGCCGCGAATGGTTACCGGACACCGGCTTTGTATCGGGTGGGACCTCGCGGAACTTAACGACCCCGGGCCTCGTCCCCATCCTCTCCACCGCGCGATCGTTCCCGAACTATCCCCACCGCAAGTTCTGCTACACCGTCCACGTCTACCGCAATGCAAAGTACATGGTCCGGACCACCTACTACTACTACGGCAGCGACTCGCCGCCGGTGTTCGACCAGATTGTGGACGGCACTTTCTGGGCCGTCGTTAACACCACCGAGGACTACGCTAACGGCATGTCGTCATACTACGAGGGGGTCTTTCTCGCCCAGGGCAAGACCATGAGCGTGTGCCTCGGGTCGAACAATTACACCGAATCCGACCCGTTTATATCCGCCCTGGAGTTTGTGATACTCGAAGACTCGCTTTACAACTCCACCGACTTTAAGTCGCACGGCCTCAGCTTGGTCGCCCGAAACGGATTTGGGTACACCGGACCCATCATTCGATACCCTGATGACCAATTTGATCGTTTTTGGGTGCCATTTGAAGAGCTCAATCCGCTGATTCCGATCAATGTGAGTAACTCAAATGCGTCAGTTTCTGGCATGTGGAATATGCCCCCTTCGAAAGTTTTTGAAAGCGAATTGACGACAGGGCAAGCTGAGGCCATGGAATTGGATTGGCCTCCTGGGTCGGTTCCTGAATCGAATTACTACATAGCTCTGTACTTTGCATCAGGGTACTCCTTGGGGTCAAGGGTGATCAACATTAGCATCAATGGTGTACAGTATTACAAGGATTTGGATGTGAAGCCAGGAGGGCTTGTTGTATATGCGACGCAGTGGCCACTTTCTGGTTCTACTAGGATTACTATGACCCCTTCTGGGAGGTCAATCGGTGGTGCTCTGATTAATGCTGGCGAGGTGTTTGATGTTCTGCCCCTTGGAGGAACAACTCTTACTCGAGATGTTATTGCTTTGCAAGGAGTAAAACAAAGTCTTCAGAACCCTCCACCTGGCTGGAGTGGTGATCCTTGTTTGCCCCGTCAGTACTCATGGACGGGCATCACATGCTCTTCAGGTCCTCGGGTTCGTGTGGTCTCTCTAAATTTGACAAGTATGGGCCTTTCAGGATCGCTATCACCTAGAATTGCCAATATGACCGCATTGTCTAATATCTTGCTTGGGAAGAATAAATTTACAGGGCCTATTCCCGATCTAAGTTCATTAATGTTACTGGAGAAATTGCACTTGGAAGACAATCATTTCAGTGGAAATATCCCCTCATCGCTCGGGAGCATTGATAGCTTGCATGAACTTTTCTTACAAAACAATTGTTTGACTGGTGAAGTTCCTGATAGCCTTGTTGGAAAATCTGGTCTGGACCTAAGGACGTCTGGAAATTCTTTTTCAGCGCCGCCACCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Malectin_like

Protein Analysis

511

Amino Acids

55.35

Weight (kDa)

5.16

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05990
fragaria_vesca FvH4_4g15050 FvH4_4g15050 FvH4_4g15050
malus_domestica MD16G1188700.v1.1
prunus_persica Prupe.1G155400_v2.0.a1 Prupe.1G155400_v2.0.a1
pyrus_communis pycom13g16260 pycom16g15870
rosa_chinensis RchiOBHm_Chr4g0416811
rosa_laevigata RLG00000007948
rosa_multiflora Rmu_sc0015663.1_g000003
rosa_roxburghii Rroxscaffold_5G00360690
rosa_rugosa Rorug04G0145100
rosa_samantha Rh4AG207600 Rh4BG204800 Rh4CG218600 Rh4DG204600
rosa_wichuraiana Rw4G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1270
AatII GACGTC 1 cut(s) 1499
AccB1I GGYRCC 2 cut(s) 95, 640
AccB7I CCANNNNNTGG 2 cut(s) 343, 956
AccI GTMKAC 1 cut(s) 264
AccII CGCG 4 cut(s) 126, 169, 212, 214
AccIII TCCGGA 1 cut(s) 288
AclWI GGATC 2 cut(s) 1127, 1243
AcoI YGGCCR 2 cut(s) 121, 947
AcsI RAATTY 3 cut(s) 1210, 1298, 1504
AcuI CTGAAG 2 cut(s) 1088, 1164
AcyI GRCGYC 2 cut(s) 96, 1496
AfaI GTAC 7 cut(s) 79, 281, 590, 830, 845, 888, 1154
AfiI CCNNNNNNNGG 7 cut(s) 143, 343, 565, 956, 987, 1049, 1118
AgsI TTSAA 2 cut(s) 650, 740
AhdI GACNNNNNGTC 1 cut(s) 547
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 5 cut(s) 429, 498, 790, 916, 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 1337, 1369
AluBI AGCT 5 cut(s) 564, 655, 764, 824, 1410
AluI AGCT 5 cut(s) 564, 655, 764, 824, 1410
Alw21I GWGCWC 2 cut(s) 657, 1009
Alw26I GTCTC 2 cut(s) 9, 1207
AlwI GGATC 2 cut(s) 1127, 1243
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 5 cut(s) 184, 455, 1065, 1188, 1394
Aor13HI TCCGGA 1 cut(s) 288
AoxI GGCC 9 cut(s) 121, 187, 360, 556, 768, 785, 947, 1225, 1307
ApeKI GCWGC 1 cut(s) 312
ApoI RAATTY 3 cut(s) 1210, 1298, 1504
ArsI GACNNNNNNTTYG 4 cut(s) 131, 163, 588, 620
AseI ATTAAT 2 cut(s) 1014, 1331
AspLEI GCGC 3 cut(s) 98, 214, 1519
AsuC2I CCSGG 2 cut(s) 185, 186
AsuHPI GGTGA 4 cut(s) 874, 1142, 1236, 1460
AsuII TTCGAA 1 cut(s) 728
AvaI CYCGRG 5 cut(s) 184, 455, 1065, 1188, 1394
AvaII GGWCC 6 cut(s) 162, 286, 291, 596, 1184, 1486
AxyI CCTNAGG 1 cut(s) 1490
BalI TGGCCA 1 cut(s) 949
BanI GGYRCC 2 cut(s) 95, 640
BanII GRGCYC 1 cut(s) 657
BbsI GAAGAC 3 cut(s) 525, 1094, 1365
Bbv12I GWGCWC 2 cut(s) 657, 1009
BbvCI CCTCAGC 2 cut(s) 560, 765
BbvI GCAGC 1 cut(s) 324
BccI CCATC 2 cut(s) 206, 608
BceAI ACGGC 7 cut(s) 86, 136, 325, 347, 364, 409, 571
BciT130I CCWGG 5 cut(s) 431, 500, 792, 918, 1119
BclI TGATCA 1 cut(s) 864
BcnI CCSGG 2 cut(s) 185, 186
BcoDI GTCTC 2 cut(s) 9, 1207
BfaI CTAG 2 cut(s) 966, 1248
BfoI RGCGCY 2 cut(s) 99, 1520
BglI GCCNNNNNGGC 1 cut(s) 922
BisI GCNGC 6 cut(s) 94, 99, 124, 313, 323, 1520
BlsI GCNGC 6 cut(s) 95, 100, 125, 314, 324, 1521
BmcAI AGTACT 1 cut(s) 1154
Bme1390I CCNGG 7 cut(s) 185, 186, 431, 500, 792, 918, 1119
Bme18I GGWCC 6 cut(s) 162, 286, 291, 596, 1184, 1486
BmeRI GACNNNNNGTC 1 cut(s) 547
BmeT110I CYCGRG 5 cut(s) 184, 455, 1065, 1188, 1394
BmgBI CACGTC 1 cut(s) 262
BmiI GGNNCC 5 cut(s) 97, 163, 598, 642, 801
BmrFI CCNGG 7 cut(s) 185, 186, 431, 500, 792, 918, 1119
BmsI GCATC 3 cut(s) 845, 885, 1176
BpiI GAAGAC 3 cut(s) 525, 1094, 1365
BpmI CTGGAG 3 cut(s) 521, 1144, 1361
Bpu10I CCTNAGC 2 cut(s) 560, 765
Bpu14I TTCGAA 1 cut(s) 728
BpuMI CCSGG 2 cut(s) 185, 186
BsaBI GATNNNNATC 2 cut(s) 671, 1240
BsaHI GRCGYC 2 cut(s) 96, 1496
BsaI GGTCTC 1 cut(s) 1207
BsaWI WCCGGW 3 cut(s) 136, 288, 593
BsaXI ACNNNNNCTCC 2 cut(s) 912, 942
Bsc4I CCNNNNNNNGG 7 cut(s) 143, 343, 565, 956, 987, 1049, 1118
Bse118I RCCGGY 2 cut(s) 143, 325
Bse1I ACTGG 2 cut(s) 1344, 1450
Bse21I CCTNAGG 1 cut(s) 1490
Bse3DI GCAATG 1 cut(s) 277
Bse8I GATNNNNATC 2 cut(s) 671, 1240
BseAI TCCGGA 1 cut(s) 288
BseBI CCWGG 5 cut(s) 431, 500, 792, 918, 1119
BseGI GGATG 2 cut(s) 198, 913
BseJI GATNNNNATC 2 cut(s) 671, 1240
BseLI CCNNNNNNNGG 7 cut(s) 143, 343, 565, 956, 987, 1049, 1118
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 574, 756
BseNI ACTGG 2 cut(s) 1344, 1450
BseRI GAGGAG 4 cut(s) 5, 14, 17, 32
BseX3I CGGCCG 1 cut(s) 121
BseXI GCAGC 1 cut(s) 324
Bsh1236I CGCG 4 cut(s) 126, 169, 212, 214
Bsh1285I CGRYCG 2 cut(s) 124, 218
BshFI GGCC 9 cut(s) 123, 189, 362, 558, 770, 787, 949, 1227, 1309
BshNI GGYRCC 2 cut(s) 95, 640
BsiEI CGRYCG 2 cut(s) 124, 218
BsiHKAI GWGCWC 2 cut(s) 657, 1009
BsiHKCI CYCGRG 5 cut(s) 184, 455, 1065, 1188, 1394
BsiSI CCGG 6 cut(s) 137, 144, 185, 289, 326, 594
BslFI GGGAC 2 cut(s) 175, 179
BslI CCNNNNNNNGG 7 cut(s) 143, 343, 565, 956, 987, 1049, 1118
BsmAI GTCTC 2 cut(s) 9, 1207
BsmFI GGGAC 2 cut(s) 175, 179
BsnI GGCC 9 cut(s) 123, 189, 362, 558, 770, 787, 949, 1227, 1309
Bso31I GGTCTC 1 cut(s) 1207
BsoBI CYCGRG 5 cut(s) 184, 455, 1065, 1188, 1394
Bsp119I TTCGAA 1 cut(s) 728
Bsp1286I GDGCHC 2 cut(s) 657, 1009
Bsp13I TCCGGA 1 cut(s) 288
Bsp1407I TGTACA 1 cut(s) 886
Bsp143I GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
Bsp19I CCATGG 1 cut(s) 771
BspANI GGCC 9 cut(s) 123, 189, 362, 558, 770, 787, 949, 1227, 1309
BspCNI CTCAG 2 cut(s) 573, 757
BspEI TCCGGA 1 cut(s) 288
BspFNI CGCG 4 cut(s) 126, 169, 212, 214
BspLI GGNNCC 5 cut(s) 97, 163, 598, 642, 801
BspPI GGATC 2 cut(s) 1127, 1243
BspQI GCTCTTC 2 cut(s) 645, 1183
BspT104I TTCGAA 1 cut(s) 728
BspT107I GGYRCC 2 cut(s) 95, 640
BspTNI GGTCTC 1 cut(s) 1207
BsrDI GCAATG 1 cut(s) 277
BsrFI RCCGGY 2 cut(s) 143, 325
BsrGI TGTACA 1 cut(s) 886
BsrI ACTGG 2 cut(s) 1344, 1450
BssAI RCCGGY 2 cut(s) 143, 325
BssMI GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
BssNI GRCGYC 2 cut(s) 96, 1496
BssT1I CCWWGG 3 cut(s) 771, 849, 1045
Bst2UI CCWGG 5 cut(s) 431, 500, 792, 918, 1119
Bst4CI ACNGT 2 cut(s) 256, 891
Bst6I CTCTTC 2 cut(s) 645, 1183
BstACI GRCGYC 2 cut(s) 96, 1496
BstAPI GCANNNNNTGC 2 cut(s) 246, 1173
BstAUI TGTACA 1 cut(s) 886
BstBI TTCGAA 1 cut(s) 728
BstC8I GCNNGC 3 cut(s) 762, 1021, 1412
BstDEI CTNAG 4 cut(s) 560, 765, 1322, 1490
BstDSI CCRYGG 1 cut(s) 771
BstEII GGTNACC 1 cut(s) 132
BstF5I GGATG 2 cut(s) 198, 913
BstFNI CGCG 4 cut(s) 126, 169, 212, 214
BstH2I RGCGCY 2 cut(s) 99, 1520
BstHHI GCGC 3 cut(s) 98, 214, 1519
BstKTI GATC 7 cut(s) 218, 631, 675, 867, 1135, 1238, 1321
BstMAI GTCTC 2 cut(s) 9, 1207
BstMBI GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
BstMCI CGRYCG 2 cut(s) 124, 218
BstMWI GCNNNNNNNGC 5 cut(s) 246, 661, 922, 1173, 1407
BstNI CCWGG 5 cut(s) 431, 500, 792, 918, 1119
BstNSI RCATGY 3 cut(s) 400, 712, 1176
BstPI GGTNACC 1 cut(s) 132
BstSCI CCNGG 7 cut(s) 183, 184, 429, 498, 790, 916, 1117
BstUI CGCG 4 cut(s) 126, 169, 212, 214
BstV1I GCAGC 1 cut(s) 324
BstV2I GAAGAC 3 cut(s) 525, 1094, 1365
BstXI CCANNNNNNTGG 2 cut(s) 778, 1122
BstZI CGGCCG 1 cut(s) 121
Bsu36I CCTNAGG 1 cut(s) 1490
BsuRI GGCC 9 cut(s) 123, 189, 362, 558, 770, 787, 949, 1227, 1309
BtgI CCRYGG 1 cut(s) 771
BtgZI GCGATG 1 cut(s) 1374
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 2 cut(s) 198, 913
BtsI GCAGTG 1 cut(s) 950
BtsIMutI CAGTG 2 cut(s) 950, 1378
Cac8I GCNNGC 3 cut(s) 762, 1021, 1412
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 3 cut(s) 98, 214, 1519
Cfr10I RCCGGY 2 cut(s) 143, 325
Cfr9I CCCGGG 1 cut(s) 184
CseI GACGC 2 cut(s) 684, 949
Csp6I GTAC 7 cut(s) 78, 280, 589, 829, 844, 887, 1153
CviAII CATG 8 cut(s) 283, 397, 442, 709, 772, 1158, 1173, 1415
CviQI GTAC 7 cut(s) 78, 280, 589, 829, 844, 887, 1153
DdeI CTNAG 4 cut(s) 560, 765, 1322, 1490
DinI GGCGCC 1 cut(s) 97
DpnI GATC 7 cut(s) 217, 630, 674, 866, 1134, 1237, 1320
DpnII GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
DrdI GACNNNNNNGTC 1 cut(s) 1270
DriI GACNNNNNGTC 1 cut(s) 547
DseDI GACNNNNNNGTC 1 cut(s) 1270
EaeI YGGCCR 2 cut(s) 121, 947
EagI CGGCCG 1 cut(s) 121
Eam1104I CTCTTC 2 cut(s) 645, 1183
Eam1105I GACNNNNNGTC 1 cut(s) 547
EarI CTCTTC 2 cut(s) 645, 1183
EciI GGCGGA 1 cut(s) 484
Ecl136II GAGCTC 1 cut(s) 655
EclXI CGGCCG 1 cut(s) 121
Eco130I CCWWGG 3 cut(s) 771, 849, 1045
Eco24I GRGCYC 1 cut(s) 657
Eco31I GGTCTC 1 cut(s) 1207
Eco47I GGWCC 6 cut(s) 162, 286, 291, 596, 1184, 1486
Eco52I CGGCCG 1 cut(s) 121
Eco53kI GAGCTC 1 cut(s) 655
Eco57I CTGAAG 2 cut(s) 1088, 1164
Eco81I CCTNAGG 1 cut(s) 1490
Eco88I CYCGRG 5 cut(s) 184, 455, 1065, 1188, 1394
Eco91I GGTNACC 1 cut(s) 132
EcoICRI GAGCTC 1 cut(s) 655
EcoO109I RGGNCCY 3 cut(s) 162, 1184, 1307
EcoO65I GGTNACC 1 cut(s) 132
EcoRII CCWGG 5 cut(s) 429, 498, 790, 916, 1117
EcoT14I CCWWGG 3 cut(s) 771, 849, 1045
EcoT38I GRGCYC 1 cut(s) 657
EgeI GGCGCC 1 cut(s) 97
EheI GGCGCC 1 cut(s) 97
ErhI CCWWGG 3 cut(s) 771, 849, 1045
FaeI CATG 8 cut(s) 286, 400, 445, 712, 775, 1161, 1176, 1418
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 2 cut(s) 175, 179
FatI CATG 8 cut(s) 282, 396, 441, 708, 771, 1157, 1172, 1414
FbaI TGATCA 1 cut(s) 864
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 6 cut(s) 94, 99, 124, 313, 323, 1520
FokI GGATG 2 cut(s) 185, 920
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 6 cut(s) 94, 99, 124, 313, 323, 1520
FspBI CTAG 2 cut(s) 966, 1248
GlaI GCGC 3 cut(s) 97, 213, 1518
GluI GCNGC 6 cut(s) 94, 99, 124, 313, 323, 1520
GsuI CTGGAG 3 cut(s) 521, 1144, 1361
HaeII RGCGCY 2 cut(s) 99, 1520
HaeIII GGCC 9 cut(s) 123, 189, 362, 558, 770, 787, 949, 1227, 1309
HapII CCGG 6 cut(s) 137, 144, 185, 289, 326, 594
HgaI GACGC 2 cut(s) 684, 949
HhaI GCGC 3 cut(s) 98, 214, 1519
Hin1I GRCGYC 2 cut(s) 96, 1496
Hin1II CATG 8 cut(s) 286, 400, 445, 712, 775, 1161, 1176, 1418
Hin6I GCGC 3 cut(s) 96, 212, 1517
HinP1I GCGC 3 cut(s) 96, 212, 1517
HincII GTYRAC 1 cut(s) 370
HindII GTYRAC 1 cut(s) 370
HinfI GANTC 5 cut(s) 317, 476, 521, 667, 806
HpaI GTTAAC 1 cut(s) 370
HpaII CCGG 6 cut(s) 137, 144, 185, 289, 326, 594
HphI GGTGA 4 cut(s) 874, 1142, 1236, 1460
Hpy166II GTNNAC 6 cut(s) 259, 265, 346, 370, 591, 887
Hpy188I TCNGA 5 cut(s) 112, 481, 672, 1011, 1107
Hpy8I GTNNAC 6 cut(s) 259, 265, 346, 370, 591, 887
Hpy99I CGWCG 3 cut(s) 122, 368, 943
HpyAV CCTTC 2 cut(s) 735, 993
HpyCH4III ACNGT 2 cut(s) 256, 891
HpyCH4IV ACGT 2 cut(s) 261, 1496
HpyCH4V TGCA 5 cut(s) 275, 836, 1084, 1351, 1414
HpyF10VI GCNNNNNNNGC 5 cut(s) 246, 661, 922, 1173, 1407
HpyF3I CTNAG 4 cut(s) 560, 765, 1322, 1490
HpySE526I ACGT 2 cut(s) 261, 1496
Hsp92I GRCGYC 2 cut(s) 96, 1496
Hsp92II CATG 8 cut(s) 286, 400, 445, 712, 775, 1161, 1176, 1418
HspAI GCGC 3 cut(s) 96, 212, 1517
KasI GGCGCC 1 cut(s) 95
Kpn2I TCCGGA 1 cut(s) 288
Ksp22I TGATCA 1 cut(s) 864
KspAI GTTAAC 1 cut(s) 370
Kzo9I GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
LguI GCTCTTC 2 cut(s) 645, 1183
LmnI GCTCC 1 cut(s) 1398
Lsp1109I GCAGC 1 cut(s) 324
LweI GCATC 3 cut(s) 845, 885, 1176
MaeI CTAG 2 cut(s) 966, 1248
MaeII ACGT 2 cut(s) 261, 1496
MaeIII GTNAC 3 cut(s) 132, 683, 1335
MalI GATC 7 cut(s) 217, 630, 674, 866, 1134, 1237, 1320
MboI GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
MboII GAAGA 6 cut(s) 530, 662, 1094, 1170, 1303, 1370
MfeI CAATTG 1 cut(s) 1435
MhlI GDGCHC 2 cut(s) 657, 1009
MlsI TGGCCA 1 cut(s) 949
MluNI TGGCCA 1 cut(s) 949
Mly113I GGCGCC 1 cut(s) 96
MlyI GAGTC 2 cut(s) 311, 515
MmeI TCCRAC 2 cut(s) 504, 1450
Mox20I TGGCCA 1 cut(s) 949
MroI TCCGGA 1 cut(s) 288
MscI TGGCCA 1 cut(s) 949
MseI TTAA 5 cut(s) 176, 369, 546, 1014, 1331
MslI CAYNNNNRTG 2 cut(s) 446, 1371
Msp20I TGGCCA 1 cut(s) 949
MspA1I CMGCKG 1 cut(s) 664
MspI CCGG 6 cut(s) 137, 144, 185, 289, 326, 594
MspR9I CCNGG 7 cut(s) 185, 186, 431, 500, 792, 918, 1119
MunI CAATTG 1 cut(s) 1435
MvaI CCWGG 5 cut(s) 431, 500, 792, 918, 1119
MvnI CGCG 4 cut(s) 126, 169, 212, 214
MwoI GCNNNNNNNGC 5 cut(s) 246, 661, 922, 1173, 1407
NarI GGCGCC 1 cut(s) 96
NciI CCSGG 2 cut(s) 185, 186
NcoI CCATGG 1 cut(s) 771
NdeII GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
NlaIII CATG 8 cut(s) 286, 400, 445, 712, 775, 1161, 1176, 1418
NlaIV GGNNCC 5 cut(s) 97, 163, 598, 642, 801
NspI RCATGY 3 cut(s) 400, 712, 1176
NspV TTCGAA 1 cut(s) 728
PaeR7I CTCGAG 1 cut(s) 1065
PasI CCCWGGG 1 cut(s) 430
PciSI GCTCTTC 2 cut(s) 645, 1183
PcsI WCGNNNNNNNCGW 1 cut(s) 123
PfeI GAWTC 3 cut(s) 476, 667, 806
PflMI CCANNNNNTGG 2 cut(s) 343, 956
PkrI GCNGC 6 cut(s) 95, 100, 125, 314, 324, 1521
Ple19I CGATCG 1 cut(s) 218
PleI GAGTC 2 cut(s) 311, 515
PluTI GGCGCC 1 cut(s) 99
PpsI GAGTC 2 cut(s) 311, 515
PpuMI RGGWCCY 2 cut(s) 162, 1184
PshBI ATTAAT 2 cut(s) 1014, 1331
Psp124BI GAGCTC 1 cut(s) 657
Psp5II RGGWCCY 2 cut(s) 162, 1184
Psp6I CCWGG 5 cut(s) 429, 498, 790, 916, 1117
PspEI GGTNACC 1 cut(s) 132
PspGI CCWGG 5 cut(s) 429, 498, 790, 916, 1117
PspN4I GGNNCC 5 cut(s) 97, 163, 598, 642, 801
PspPPI RGGWCCY 2 cut(s) 162, 1184
PsrI GAACNNNNNNTAC 4 cut(s) 1045, 1077, 1410, 1442
PstNI CAGNNNCTG 1 cut(s) 704
PvuI CGATCG 1 cut(s) 218
RsaI GTAC 7 cut(s) 79, 281, 590, 830, 845, 888, 1154
RsaNI GTAC 7 cut(s) 78, 280, 589, 829, 844, 887, 1153
RseI CAYNNNNRTG 2 cut(s) 446, 1371
SacI GAGCTC 1 cut(s) 657
SapI GCTCTTC 2 cut(s) 645, 1183
SaqAI TTAA 5 cut(s) 176, 369, 546, 1014, 1331
SatI GCNGC 6 cut(s) 94, 99, 124, 313, 323, 1520
Sau3AI GATC 7 cut(s) 215, 628, 672, 864, 1132, 1235, 1318
ScaI AGTACT 1 cut(s) 1154
SchI GAGTC 2 cut(s) 311, 515
ScrFI CCNGG 7 cut(s) 185, 186, 431, 500, 792, 918, 1119
SduI GDGCHC 2 cut(s) 657, 1009
SfaNI GCATC 3 cut(s) 845, 885, 1176
SfoI GGCGCC 1 cut(s) 97
Sfr274I CTCGAG 1 cut(s) 1065
SfuI TTCGAA 1 cut(s) 728
SgrAI CRCCGGYG 1 cut(s) 325
SinI GGWCC 6 cut(s) 162, 286, 291, 596, 1184, 1486
SlaI CTCGAG 1 cut(s) 1065
SmaI CCCGGG 1 cut(s) 186
SmiMI CAYNNNNRTG 2 cut(s) 446, 1371
SmlI CTYRAG 1 cut(s) 1065
SmoI CTYRAG 1 cut(s) 1065
SspDI GGCGCC 1 cut(s) 95
SspMI CTAG 2 cut(s) 966, 1248
SstI GAGCTC 1 cut(s) 657
StyD4I CCNGG 7 cut(s) 183, 184, 429, 498, 790, 916, 1117
StyI CCWWGG 3 cut(s) 771, 849, 1045
TaaI ACNGT 2 cut(s) 256, 891
TaiI ACGT 2 cut(s) 264, 1499
TaqI TCGA 8 cut(s) 117, 333, 461, 516, 607, 728, 809, 1066
TatI WGTACW 4 cut(s) 279, 828, 886, 1152
TauI GCSGC 5 cut(s) 96, 101, 126, 325, 1522
TfiI GAWTC 3 cut(s) 476, 667, 806
Tru1I TTAA 5 cut(s) 176, 369, 546, 1014, 1331
Tru9I TTAA 5 cut(s) 176, 369, 546, 1014, 1331
TscAI CASTG 2 cut(s) 950, 1378
TseI GCWGC 1 cut(s) 312
TspDTI ATGAA 2 cut(s) 1317, 1431
TspGWI ACGGA 1 cut(s) 594
TspMI CCCGGG 1 cut(s) 184
TspRI CASTG 2 cut(s) 950, 1378
Van91I CCANNNNNTGG 2 cut(s) 343, 956
VpaK11BI GGWCC 6 cut(s) 162, 286, 291, 596, 1184, 1486
VspI ATTAAT 2 cut(s) 1014, 1331
XapI RAATTY 3 cut(s) 1210, 1298, 1504
XceI RCATGY 3 cut(s) 400, 712, 1176
XhoI CTCGAG 1 cut(s) 1065
XmaI CCCGGG 1 cut(s) 184
XmiI GTMKAC 1 cut(s) 264
XspI CTAG 2 cut(s) 966, 1248
ZraI GACGTC 1 cut(s) 1497
ZrmI AGTACT 1 cut(s) 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.