pycom16g15870

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012374
Physical Location & Seq
Forward (+)
11788749 .. 11792762
4014 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g15870.2

Sequence Viewer

Length: 1761 bp
ATGTTGTGGGGTCAACAATTAAACTACCGACTCTTGACCAAGCCCTTGTTTTCCCGCGTTTACCCAACCGAAATACCTTTCCTCTTCCCCAACTCCACCTTCGCGGCTCCTTCTCTCTCTCTCTCTCTCTCTCTCTGCCTATTCCTTCCTTGTTTATCTCACTCACCCAACCAAAGCCCTTCCATTCTCTCTCTACACCTCCTCTCTCTCTCCTCACCCTCCACCATGTTTATCTCCCTCCTCCATCTCCTCCTCCCCCTCCTCCTCCTCTCCCTCATCCCCCTCTCTCTCTCCCTCCCCAAAGGTACTCTTATTAACTGCGGCGCCGCCGTCAAATCCGAAATCGACGGCCGCGAATGGCTCCCGGACACCAGCTTCGTATCCGCTGGGACCCCGAGGAACTTAACGACCCCGGTCCTCGTCCCCATCCTCTCCACCGCCCGATCGTTCCCGAACAATCCCCACCGCAAGTTCTGCTACACCGTCCAGGTCTACCGCAAAGCTAAGTACATGGTCCGGACCACGTACTACTACTACGGCAACGGCTCGCCGCCGGTGTTCGACCAGATTGTTGACGGCACTTTCTGGGCCGTCGTTAACACCACCGAAGACTACGCCAAGGGCTTGTCGTCTTACTACGAGGGGGTCTTTCTCGCTCAGGGCAAGACCATGAGCGTCTGTCTCGGGTCGAACAATTACACCAAATCCGACCCGTTTATCTCCGCCCTGGAGTTTGTGATACTCGAAGACTCGCTCTACAACTCCACGGACTTCAAGTCGTACGGCCTCAGTTTGGTCTCCCGAAACAGCTTTGGGTACACCGGATCCATCATTCGATACCCAGATGACCAATTCGATCGATTTTGGGCGCCATTTGAAGAACACAATCCAATGATTCCGATCAATGTGAGCAACTCAAATGCGTCTGTTTCTGGCATGTGGAATATGCCCCCTTTGAAAGTTTTTGAAAGCGAATTGACGACAGGAAAAACTGAGGCCATGGAGTTGAATTGGCCTCCTCGGTCAGTTCCTGAATCGAATTACTACATAGCTTTGTACTTTGCCTCAGGGCAGTCCACAGGGTCAAGAGTGTTGAACATTAGCATCAATGGTATTCCGTATTACAAGGATTTGGATGTGAAGCCAGAAGGGCTTGTTGTTTATGCGGCAAGGTGGCCTCTTTCTGGTTCTACTAGGATTACTTTGACCCCATCTGCCGGATCAAGCAGTGGTGCTTTGATTAATGCAGGCGAGGTGTTTGATGTGTTGCCCCTTGGAGGAACAACTCTTACTCGAGATGTTATTGCTTTGCAAAGAGTAAAGCAAAGTCTTCTGAACCCTCCACCTGACTGGAATGGTGATCCTTGTTTTCCCCATCAGTACTCGTGGACAGGCATTACATGCTCTCCAGGCCCTCGAGTTCGTGTGGTCACTTTAAATCTGACAAATATGGGCCTTTCAGGATCGCTATCACCTAGCATTGCCAACATGACCGCATTGTCTAATATCTTGCTTGGGAAGAATAACTTTACAGGGCATATTCCCGATCTAAGTTCGTTGAAGTTACTGGAGAAACTGCACTTGGAAAACAATCATTTCAGTGGAAATATCCCCTCATCGCTCGGGAGCATTGATAGGTTGCATGAACTTTTCTTACAAAACAATAATTTGACTGGTGAAGTTCCAGATAGCCTTATTCGAAAATCGGGACTGGACCTAAGGACGTTTGGAAATTCTTTTTCAGCACAGCCACCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Malectin_like

Protein Analysis

587

Amino Acids

64.46

Weight (kDa)

7.13

Isoelectric Point (pI)

39.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05990
fragaria_vesca FvH4_4g15050 FvH4_4g15050 FvH4_4g15050
malus_domestica MD16G1188700.v1.1
prunus_persica Prupe.1G155400_v2.0.a1 Prupe.1G155400_v2.0.a1
pyrus_communis pycom13g16260 pycom16g15870
rosa_chinensis RchiOBHm_Chr4g0416811
rosa_laevigata RLG00000007948
rosa_multiflora Rmu_sc0015663.1_g000003
rosa_roxburghii Rroxscaffold_5G00360690
rosa_rugosa Rorug04G0145100
rosa_samantha Rh4AG207600 Rh4BG204800 Rh4CG218600 Rh4DG204600
rosa_wichuraiana Rw4G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1498
AccB1I GGYRCC 2 cut(s) 323, 868
AccI GTMKAC 1 cut(s) 492
AccII CGCG 3 cut(s) 57, 104, 354
AccIII TCCGGA 1 cut(s) 516
AclWI GGATC 5 cut(s) 819, 832, 1228, 1355, 1471
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 1 cut(s) 1732
AcyI GRCGYC 2 cut(s) 324, 869
AfaI GTAC 7 cut(s) 307, 509, 527, 782, 818, 1058, 1382
AfiI CCNNNNNNNGG 4 cut(s) 793, 1184, 1217, 1277
AgsI TTSAA 7 cut(s) 775, 878, 958, 968, 1009, 1096, 1561
AhdI GACNNNNNGTC 1 cut(s) 775
AjnI CCWGG 3 cut(s) 486, 726, 1408
AjuI GAANNNNNNNTTGG 4 cut(s) 83, 115, 1565, 1597
AluBI AGCT 4 cut(s) 375, 503, 810, 1052
AluI AGCT 4 cut(s) 375, 503, 810, 1052
Alw26I GTCTC 2 cut(s) 686, 802
AlwI GGATC 5 cut(s) 819, 832, 1228, 1355, 1471
AlwNI CAGNNNCTG 1 cut(s) 1031
Ama87I CYCGRG 5 cut(s) 394, 683, 1293, 1416, 1622
Aor13HI TCCGGA 1 cut(s) 516
AoxI GGCC 8 cut(s) 349, 588, 784, 996, 1013, 1175, 1411, 1453
ApoI RAATTY 1 cut(s) 1732
ArsI GACNNNNNNTTYG 2 cut(s) 359, 391
AseI ATTAAT 1 cut(s) 1242
AspLEI GCGC 2 cut(s) 326, 871
AspS9I GGNCC 8 cut(s) 390, 415, 514, 519, 588, 1412, 1453, 1714
AsuC2I CCSGG 2 cut(s) 365, 413
AsuHPI GGTGA 5 cut(s) 156, 207, 1370, 1464, 1688
AsuII TTCGAA 1 cut(s) 1699
AvaI CYCGRG 5 cut(s) 394, 683, 1293, 1416, 1622
AvaII GGWCC 5 cut(s) 390, 415, 514, 519, 1714
AxyI CCTNAGG 2 cut(s) 1066, 1718
BamHI GGATCC 1 cut(s) 824
BanI GGYRCC 2 cut(s) 323, 868
BauI CACGAG 1 cut(s) 1384
BbsI GAAGAC 3 cut(s) 615, 753, 1322
BccI CCATC 5 cut(s) 252, 434, 836, 1219, 1383
BceAI ACGGC 7 cut(s) 314, 364, 553, 559, 575, 592, 799
BciT130I CCWGG 3 cut(s) 488, 728, 1410
BciVI GTATCC 1 cut(s) 391
BcnI CCSGG 2 cut(s) 365, 413
BcoDI GTCTC 2 cut(s) 686, 802
BfaI CTAG 2 cut(s) 1194, 1476
BfoI RGCGCY 2 cut(s) 327, 872
BfuI GTATCC 1 cut(s) 391
BglI GCCNNNNNGGC 1 cut(s) 1150
BisI GCNGC 6 cut(s) 105, 322, 327, 352, 551, 1167
BlsI GCNGC 6 cut(s) 106, 323, 328, 353, 552, 1168
BmcAI AGTACT 1 cut(s) 1382
Bme1390I CCNGG 5 cut(s) 365, 413, 488, 728, 1410
Bme18I GGWCC 5 cut(s) 390, 415, 514, 519, 1714
BmeRI GACNNNNNGTC 1 cut(s) 775
BmeT110I CYCGRG 5 cut(s) 394, 683, 1293, 1416, 1622
BmgT120I GGNCC 8 cut(s) 390, 415, 514, 519, 588, 1412, 1453, 1714
BmiI GGNNCC 7 cut(s) 108, 325, 362, 391, 392, 826, 870
BmrFI CCNGG 5 cut(s) 365, 413, 488, 728, 1410
BmsI GCATC 1 cut(s) 1113
BoxI GACNNNNGTC 1 cut(s) 413
BpiI GAAGAC 3 cut(s) 615, 753, 1322
BpmI CTGGAG 3 cut(s) 749, 1392, 1589
Bpu10I CCTNAGC 1 cut(s) 657
Bpu14I TTCGAA 1 cut(s) 1699
BpuMI CCSGG 2 cut(s) 365, 413
Bsa29I ATCGAT 1 cut(s) 859
BsaAI YACGTR 1 cut(s) 525
BsaBI GATNNNNATC 2 cut(s) 899, 1468
BsaHI GRCGYC 2 cut(s) 324, 869
BsaI GGTCTC 1 cut(s) 802
BsaJI CCNNGG 8 cut(s) 395, 411, 618, 726, 765, 999, 1019, 1273
BsaWI WCCGGW 2 cut(s) 516, 821
BsaXI ACNNNNNCTCC 2 cut(s) 1390, 1420
Bsc4I CCNNNNNNNGG 4 cut(s) 793, 1184, 1217, 1277
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 4 cut(s) 1355, 1572, 1678, 1716
Bse21I CCTNAGG 2 cut(s) 1066, 1718
Bse3DI GCAATG 1 cut(s) 1479
Bse8I GATNNNNATC 2 cut(s) 899, 1468
BseAI TCCGGA 1 cut(s) 516
BseBI CCWGG 3 cut(s) 488, 728, 1410
BseCI ATCGAT 1 cut(s) 859
BseDI CCNNGG 8 cut(s) 395, 411, 618, 726, 765, 999, 1019, 1273
BseGI GGATG 3 cut(s) 276, 426, 1141
BseJI GATNNNNATC 2 cut(s) 899, 1468
BseLI CCNNNNNNNGG 4 cut(s) 793, 1184, 1217, 1277
BseMI GCAATG 1 cut(s) 1479
BseMII CTCAG 4 cut(s) 671, 802, 984, 1080
BseNI ACTGG 4 cut(s) 1355, 1572, 1678, 1716
BseRI GAGGAG 9 cut(s) 191, 202, 230, 239, 242, 251, 254, 257, 1008
BseX3I CGGCCG 1 cut(s) 349
BseYI CCCAGC 1 cut(s) 386
BsgI GTGCAG 1 cut(s) 1562
Bsh1236I CGCG 3 cut(s) 57, 104, 354
Bsh1285I CGRYCG 3 cut(s) 352, 446, 859
BshFI GGCC 8 cut(s) 351, 590, 786, 998, 1015, 1177, 1413, 1455
BshNI GGYRCC 2 cut(s) 323, 868
BshVI ATCGAT 1 cut(s) 859
BsiEI CGRYCG 3 cut(s) 352, 446, 859
BsiHKCI CYCGRG 5 cut(s) 394, 683, 1293, 1416, 1622
BsiSI CCGG 6 cut(s) 365, 413, 517, 554, 822, 1218
BsiWI CGTACG 1 cut(s) 780
BslFI GGGAC 3 cut(s) 403, 407, 1722
BslI CCNNNNNNNGG 4 cut(s) 793, 1184, 1217, 1277
BsmAI GTCTC 2 cut(s) 686, 802
BsmFI GGGAC 3 cut(s) 403, 407, 1722
BsnI GGCC 8 cut(s) 351, 590, 786, 998, 1015, 1177, 1413, 1455
Bso31I GGTCTC 1 cut(s) 802
BsoBI CYCGRG 5 cut(s) 394, 683, 1293, 1416, 1622
Bsp119I TTCGAA 1 cut(s) 1699
Bsp13I TCCGGA 1 cut(s) 516
Bsp143I GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
Bsp19I CCATGG 1 cut(s) 999
BspANI GGCC 8 cut(s) 351, 590, 786, 998, 1015, 1177, 1413, 1455
BspCNI CTCAG 4 cut(s) 670, 801, 985, 1079
BspDI ATCGAT 1 cut(s) 859
BspEI TCCGGA 1 cut(s) 516
BspFNI CGCG 3 cut(s) 57, 104, 354
BspLI GGNNCC 7 cut(s) 108, 325, 362, 391, 392, 826, 870
BspPI GGATC 5 cut(s) 819, 832, 1228, 1355, 1471
BspT104I TTCGAA 1 cut(s) 1699
BspT107I GGYRCC 2 cut(s) 323, 868
BspTNI GGTCTC 1 cut(s) 802
BsrDI GCAATG 1 cut(s) 1479
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 4 cut(s) 1355, 1572, 1678, 1716
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 8 cut(s) 395, 411, 618, 726, 765, 999, 1019, 1273
BssMI GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
BssNI GRCGYC 2 cut(s) 324, 869
BssSI CACGAG 1 cut(s) 1384
BssT1I CCWWGG 3 cut(s) 618, 999, 1273
Bst2BI CACGAG 1 cut(s) 1384
Bst2UI CCWGG 3 cut(s) 488, 728, 1410
Bst4CI ACNGT 1 cut(s) 484
Bst6I CTCTTC 1 cut(s) 89
BstACI GRCGYC 2 cut(s) 324, 869
BstAPI GCANNNNNTGC 2 cut(s) 474, 1401
BstBAI YACGTR 1 cut(s) 525
BstBI TTCGAA 1 cut(s) 1699
BstC8I GCNNGC 2 cut(s) 548, 1249
BstDEI CTNAG 7 cut(s) 504, 657, 788, 993, 1066, 1550, 1718
BstDSI CCRYGG 2 cut(s) 765, 999
BstF5I GGATG 3 cut(s) 276, 426, 1141
BstFNI CGCG 3 cut(s) 57, 104, 354
BstH2I RGCGCY 2 cut(s) 327, 872
BstHHI GCGC 2 cut(s) 326, 871
BstKTI GATC 8 cut(s) 446, 827, 859, 903, 1223, 1363, 1466, 1549
BstMAI GTCTC 2 cut(s) 686, 802
BstMBI GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
BstMCI CGRYCG 3 cut(s) 352, 446, 859
BstMWI GCNNNNNNNGC 4 cut(s) 474, 1150, 1401, 1410
BstNI CCWGG 3 cut(s) 488, 728, 1410
BstNSI RCATGY 2 cut(s) 940, 1404
BstPAI GACNNNNGTC 1 cut(s) 413
BstSCI CCNGG 5 cut(s) 363, 411, 486, 726, 1408
BstUI CGCG 3 cut(s) 57, 104, 354
BstV2I GAAGAC 3 cut(s) 615, 753, 1322
BstX2I RGATCY 1 cut(s) 824
BstXI CCANNNNNNTGG 1 cut(s) 1350
BstYI RGATCY 1 cut(s) 824
BstZI CGGCCG 1 cut(s) 349
Bsu15I ATCGAT 1 cut(s) 859
Bsu36I CCTNAGG 2 cut(s) 1066, 1718
BsuI GTATCC 1 cut(s) 391
BsuRI GGCC 8 cut(s) 351, 590, 786, 998, 1015, 1177, 1413, 1455
BsuTUI ATCGAT 1 cut(s) 859
BtgI CCRYGG 2 cut(s) 765, 999
BtgZI GCGATG 1 cut(s) 1602
BtsCI GGATG 3 cut(s) 276, 426, 1141
BtsI GCAGTG 1 cut(s) 1234
BtsIMutI CAGTG 2 cut(s) 1234, 1606
Cac8I GCNNGC 2 cut(s) 548, 1249
CaiI CAGNNNCTG 1 cut(s) 1031
CfoI GCGC 2 cut(s) 326, 871
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 8 cut(s) 390, 415, 514, 519, 588, 1412, 1453, 1714
ClaI ATCGAT 1 cut(s) 859
CseI GACGC 2 cut(s) 664, 912
Csp6I GTAC 7 cut(s) 306, 508, 526, 781, 817, 1057, 1381
CviAII CATG 8 cut(s) 226, 511, 670, 937, 1000, 1401, 1489, 1643
CviQI GTAC 7 cut(s) 306, 508, 526, 781, 817, 1057, 1381
DdeI CTNAG 7 cut(s) 504, 657, 788, 993, 1066, 1550, 1718
DinI GGCGCC 2 cut(s) 325, 870
DpnI GATC 8 cut(s) 445, 826, 858, 902, 1222, 1362, 1465, 1548
DpnII GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
DraI TTTAAA 1 cut(s) 1437
DrdI GACNNNNNNGTC 1 cut(s) 1498
DriI GACNNNNNGTC 1 cut(s) 775
DseDI GACNNNNNNGTC 1 cut(s) 1498
EaeI YGGCCR 1 cut(s) 349
EagI CGGCCG 1 cut(s) 349
Eam1104I CTCTTC 1 cut(s) 89
Eam1105I GACNNNNNGTC 1 cut(s) 775
EarI CTCTTC 1 cut(s) 89
EciI GGCGGA 1 cut(s) 712
EclXI CGGCCG 1 cut(s) 349
Eco130I CCWWGG 3 cut(s) 618, 999, 1273
Eco31I GGTCTC 1 cut(s) 802
Eco47I GGWCC 5 cut(s) 390, 415, 514, 519, 1714
Eco52I CGGCCG 1 cut(s) 349
Eco81I CCTNAGG 2 cut(s) 1066, 1718
Eco88I CYCGRG 5 cut(s) 394, 683, 1293, 1416, 1622
EcoO109I RGGNCCY 2 cut(s) 390, 1412
EcoRII CCWGG 3 cut(s) 486, 726, 1408
EcoT14I CCWWGG 3 cut(s) 618, 999, 1273
EgeI GGCGCC 2 cut(s) 325, 870
EheI GGCGCC 2 cut(s) 325, 870
ErhI CCWWGG 3 cut(s) 618, 999, 1273
FaeI CATG 8 cut(s) 229, 514, 673, 940, 1003, 1404, 1492, 1646
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 1511, 1543
FaqI GGGAC 3 cut(s) 403, 407, 1722
FatI CATG 8 cut(s) 225, 510, 669, 936, 999, 1400, 1488, 1642
FauI CCCGC 1 cut(s) 62
FblI GTMKAC 1 cut(s) 492
Fnu4HI GCNGC 6 cut(s) 105, 322, 327, 352, 551, 1167
FokI GGATG 3 cut(s) 263, 413, 1148
Fsp4HI GCNGC 6 cut(s) 105, 322, 327, 352, 551, 1167
FspBI CTAG 2 cut(s) 1194, 1476
GlaI GCGC 2 cut(s) 325, 870
GluI GCNGC 6 cut(s) 105, 322, 327, 352, 551, 1167
GsaI CCCAGC 1 cut(s) 390
GsuI CTGGAG 3 cut(s) 749, 1392, 1589
HaeII RGCGCY 2 cut(s) 327, 872
HaeIII GGCC 8 cut(s) 351, 590, 786, 998, 1015, 1177, 1413, 1455
HapII CCGG 6 cut(s) 365, 413, 517, 554, 822, 1218
HgaI GACGC 2 cut(s) 664, 912
HhaI GCGC 2 cut(s) 326, 871
Hin1I GRCGYC 2 cut(s) 324, 869
Hin1II CATG 8 cut(s) 229, 514, 673, 940, 1003, 1404, 1492, 1646
Hin6I GCGC 2 cut(s) 324, 869
HinP1I GCGC 2 cut(s) 324, 869
HincII GTYRAC 3 cut(s) 14, 574, 598
HindII GTYRAC 3 cut(s) 14, 574, 598
HinfI GANTC 4 cut(s) 30, 749, 895, 1034
HpaI GTTAAC 1 cut(s) 598
HpaII CCGG 6 cut(s) 365, 413, 517, 554, 822, 1218
HphI GGTGA 5 cut(s) 156, 207, 1370, 1464, 1688
Hpy166II GTNNAC 8 cut(s) 14, 61, 493, 574, 598, 819, 1077, 1389
Hpy188I TCNGA 5 cut(s) 340, 709, 900, 1335, 1443
Hpy8I GTNNAC 8 cut(s) 14, 61, 493, 574, 598, 819, 1077, 1389
Hpy99I CGWCG 2 cut(s) 350, 596
HpyAV CCTTC 5 cut(s) 109, 120, 155, 189, 1142
HpyCH4III ACNGT 1 cut(s) 484
HpyCH4IV ACGT 2 cut(s) 524, 1724
HpyCH4V TGCA 4 cut(s) 1247, 1312, 1579, 1642
HpyF10VI GCNNNNNNNGC 4 cut(s) 474, 1150, 1401, 1410
HpyF3I CTNAG 7 cut(s) 504, 657, 788, 993, 1066, 1550, 1718
HpySE526I ACGT 2 cut(s) 524, 1724
Hsp92I GRCGYC 2 cut(s) 324, 869
Hsp92II CATG 8 cut(s) 229, 514, 673, 940, 1003, 1404, 1492, 1646
HspAI GCGC 2 cut(s) 324, 869
KasI GGCGCC 2 cut(s) 323, 868
KflI GGGWCCC 1 cut(s) 390
Kpn2I TCCGGA 1 cut(s) 516
KspAI GTTAAC 1 cut(s) 598
Kzo9I GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
LmnI GCTCC 3 cut(s) 112, 366, 1626
LweI GCATC 1 cut(s) 1113
MaeI CTAG 2 cut(s) 1194, 1476
MaeII ACGT 2 cut(s) 524, 1724
MaeIII GTNAC 2 cut(s) 1429, 1563
MalI GATC 8 cut(s) 445, 826, 858, 902, 1222, 1362, 1465, 1548
MboI GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
MboII GAAGA 6 cut(s) 76, 620, 758, 890, 1322, 1531
MflI RGATCY 1 cut(s) 824
MluCI AATT 8 cut(s) 17, 694, 851, 974, 1009, 1039, 1666, 1732
Mly113I GGCGCC 2 cut(s) 324, 869
MlyI GAGTC 2 cut(s) 24, 743
MmeI TCCRAC 1 cut(s) 732
MroI TCCGGA 1 cut(s) 516
MseI TTAA 6 cut(s) 20, 315, 404, 597, 1242, 1436
MslI CAYNNNNRTG 1 cut(s) 1599
MspA1I CMGCKG 1 cut(s) 386
MspI CCGG 6 cut(s) 365, 413, 517, 554, 822, 1218
MspR9I CCNGG 5 cut(s) 365, 413, 488, 728, 1410
MvaI CCWGG 3 cut(s) 488, 728, 1410
MvnI CGCG 3 cut(s) 57, 104, 354
MwoI GCNNNNNNNGC 4 cut(s) 474, 1150, 1401, 1410
NarI GGCGCC 2 cut(s) 324, 869
NciI CCSGG 2 cut(s) 365, 413
NcoI CCATGG 1 cut(s) 999
NdeII GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
NlaIII CATG 8 cut(s) 229, 514, 673, 940, 1003, 1404, 1492, 1646
NlaIV GGNNCC 7 cut(s) 108, 325, 362, 391, 392, 826, 870
NmuCI GTSAC 1 cut(s) 1429
NspI RCATGY 2 cut(s) 940, 1404
NspV TTCGAA 1 cut(s) 1699
PaeR7I CTCGAG 2 cut(s) 1293, 1416
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PfeI GAWTC 2 cut(s) 895, 1034
Pfl23II CGTACG 1 cut(s) 780
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 6 cut(s) 106, 323, 328, 353, 552, 1168
Ple19I CGATCG 2 cut(s) 446, 859
PleI GAGTC 2 cut(s) 24, 743
PluTI GGCGCC 2 cut(s) 327, 872
PpsI GAGTC 2 cut(s) 24, 743
Ppu21I YACGTR 1 cut(s) 525
PpuMI RGGWCCY 1 cut(s) 390
PshAI GACNNNNGTC 1 cut(s) 413
PshBI ATTAAT 1 cut(s) 1242
Psp5II RGGWCCY 1 cut(s) 390
Psp6I CCWGG 3 cut(s) 486, 726, 1408
PspFI CCCAGC 1 cut(s) 386
PspGI CCWGG 3 cut(s) 486, 726, 1408
PspLI CGTACG 1 cut(s) 780
PspN4I GGNNCC 7 cut(s) 108, 325, 362, 391, 392, 826, 870
PspPI GGNCC 8 cut(s) 390, 415, 514, 519, 588, 1412, 1453, 1714
PspPPI RGGWCCY 1 cut(s) 390
PspXI VCTCGAGB 1 cut(s) 1416
PsrI GAACNNNNNNTAC 4 cut(s) 1273, 1305, 1638, 1670
PstNI CAGNNNCTG 1 cut(s) 1031
PsuI RGATCY 1 cut(s) 824
PvuI CGATCG 2 cut(s) 446, 859
RsaI GTAC 7 cut(s) 307, 509, 527, 782, 818, 1058, 1382
RsaNI GTAC 7 cut(s) 306, 508, 526, 781, 817, 1057, 1381
RseI CAYNNNNRTG 1 cut(s) 1599
SaqAI TTAA 6 cut(s) 20, 315, 404, 597, 1242, 1436
SatI GCNGC 6 cut(s) 105, 322, 327, 352, 551, 1167
Sau3AI GATC 8 cut(s) 443, 824, 856, 900, 1220, 1360, 1463, 1546
Sau96I GGNCC 8 cut(s) 390, 415, 514, 519, 588, 1412, 1453, 1714
ScaI AGTACT 1 cut(s) 1382
SchI GAGTC 2 cut(s) 24, 743
ScrFI CCNGG 5 cut(s) 365, 413, 488, 728, 1410
SfaNI GCATC 1 cut(s) 1113
SfoI GGCGCC 2 cut(s) 325, 870
Sfr274I CTCGAG 2 cut(s) 1293, 1416
SfuI TTCGAA 1 cut(s) 1699
SgrAI CRCCGGYG 1 cut(s) 553
SinI GGWCC 5 cut(s) 390, 415, 514, 519, 1714
SlaI CTCGAG 2 cut(s) 1293, 1416
SmiMI CAYNNNNRTG 1 cut(s) 1599
SmlI CTYRAG 2 cut(s) 1293, 1416
SmoI CTYRAG 2 cut(s) 1293, 1416
Sse9I AATT 8 cut(s) 17, 694, 851, 974, 1009, 1039, 1666, 1732
SspDI GGCGCC 2 cut(s) 323, 868
SspMI CTAG 2 cut(s) 1194, 1476
StyD4I CCNGG 5 cut(s) 363, 411, 486, 726, 1408
StyI CCWWGG 3 cut(s) 618, 999, 1273
TaaI ACNGT 1 cut(s) 484
TaiI ACGT 2 cut(s) 527, 1727
TaqII GACCGA 1 cut(s) 1011
TasI AATT 8 cut(s) 17, 694, 851, 974, 1009, 1039, 1666, 1732
TatI WGTACW 3 cut(s) 507, 1056, 1380
TauI GCSGC 6 cut(s) 107, 324, 329, 354, 553, 1169
TfiI GAWTC 2 cut(s) 895, 1034
Tru1I TTAA 6 cut(s) 20, 315, 404, 597, 1242, 1436
Tru9I TTAA 6 cut(s) 20, 315, 404, 597, 1242, 1436
TscAI CASTG 2 cut(s) 1234, 1606
TseFI GTSAC 1 cut(s) 1429
Tsp45I GTSAC 1 cut(s) 1429
TspDTI ATGAA 1 cut(s) 1659
TspGWI ACGGA 2 cut(s) 782, 1107
TspRI CASTG 2 cut(s) 1234, 1606
VpaK11BI GGWCC 5 cut(s) 390, 415, 514, 519, 1714
VspI ATTAAT 1 cut(s) 1242
XapI RAATTY 1 cut(s) 1732
XceI RCATGY 2 cut(s) 940, 1404
XhoI CTCGAG 2 cut(s) 1293, 1416
XmiI GTMKAC 1 cut(s) 492
XspI CTAG 2 cut(s) 1194, 1476
ZrmI AGTACT 1 cut(s) 1382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.