Rroxscaffold_5G00360690

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0012374
Physical Location & Seq
Reverse (-)
41297143 .. 41301212
4070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00360690.1

Sequence Viewer

Length: 1509 bp
ATGCCCCTCCCCCTCCCCCTCCTCCTCCTCCTCTCCCTCCTCCCCATCTCTCTTTCTCAACCCAAAGGTACTTTGATCAACTGCGGCGCAGTGGTCGAATCCAAAATTGACGGCCGCGAATGGCTTCCCGACTCCGGCTTCATCTCCGGCGGTGGAACCCCGAAAACGCTGAACATTTCAGGCCTGGTCCCTATCCTGACCACTCTCCGATCGTTCCCGAACATTGCCGGCCGGAAGTACTGCTACAACGTCCAGGTCTACCGCAGCGCCAAGTACATGGTCCGGACCACTTACTACTACGGCGGAATCAACGGCCGGAGCTCGCCGCCGGTGTTCGACCAGATCATCGACGGCACCTTCTGGGCCGTCGTCAACACCACCGAGGACTACGTCAACGGCATGTCGTCTTATTATGAGGGGGTGTTTCTCGCGCAGGGTAAGACCATGAGCGTCTGCGTCGGGGCGAATACGTATACGGACTCCGACCCATTTATATCCGCTTTGGAGTTTGTGATTCTCCAGGACTCGCTGTACAACTCTACGGATTTCAAGTCCAATGGACTCAGCTTGGTCGCTAGGCATAGCTTTGGGTACAATGGACCCATCATTAGATACCCAGATGATCAGTTTGATCGAATCTGGGAGCCATTTGGAGAAACCATTAATGACTCAAGCAATGCAAATGTATCTGTTTCTGGGTTATGGAACTTGCCCCCTTTGAAGGTTTTCGAAAGCGAGGTGACGACTAACCAGTCCCAACCGATCGAGTTGAATTGGCCTCCAAAGCCGGTTCAGGATGCAAACTACTACATTGCACTGTACTTTGCGGATTCAAGGGATAGAGTTTTCAGCATATTCATTAATAGTGTGCCATATTACAAAGATTTGAATGTGACAGAAGCAGGGCTTGTGGTCTATGCCACCAAGTGGCCTCTTGGTGGTACTACAAATATTACTTTGACCCCTTCTTCTAGGTCAGTACTTGGTCCTTCGATTAATGCTGGAGAGGCTTTTCAGGTGTTGCCCCTCGGAGGAAGAACTCTTACTCGAGATGTTATTGCTTTGGAAAGAGTAAAAGAGAGTCTTGAGAACCCTCCACCTAACTGGAATGGTGATCCCTGTCTTCCCCGTCAGTACACGTGGACAGGCATTACATGCTCTGGAGTTCGTGTGGTCTCTTTAAATCTAACAAATATGGGCCTTTCTGGTTCACTGTCTCCGAGTATTGCCAATATGACTGCATTGTCTGACATCTGGCTTGGGAATAACAATTTGTCAGGACCTATTCCTGATCTCAGTTCATTGAAGATACTGGAAACACTGCACTTGGAAGACAATCAATTCAGTGGAGTTATTCCCTCATCGCTTGGGAACATTGATAGCTTGCGTGAACTCTTTTTACAAAACAATAATCTCACTGGTCAAGTTCCTAACAGCCTTACTGGAAAGTCTGGGTTGAATCTAAGGATTTCTCCAGGAAATTCTTTTTCAGCACCCCCACCTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 Malectin_like

Protein Analysis

502

Amino Acids

54.72

Weight (kDa)

5.01

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 26 - 340 5.7e-58 Malectin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012374)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05990
fragaria_vesca FvH4_4g15050 FvH4_4g15050 FvH4_4g15050
malus_domestica MD16G1188700.v1.1
prunus_persica Prupe.1G155400_v2.0.a1 Prupe.1G155400_v2.0.a1
pyrus_communis pycom13g16260 pycom16g15870
rosa_chinensis RchiOBHm_Chr4g0416811
rosa_laevigata RLG00000007948
rosa_multiflora Rmu_sc0015663.1_g000003
rosa_roxburghii Rroxscaffold_5G00360690
rosa_rugosa Rorug04G0145100
rosa_samantha Rh4AG207600 Rh4BG204800 Rh4CG218600 Rh4DG204600
rosa_wichuraiana Rw4G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 751, 1243
AccB1I GGYRCC 1 cut(s) 353
AccB7I CCANNNNNTGG 1 cut(s) 927
AccI GTMKAC 2 cut(s) 258, 473
AccII CGCG 2 cut(s) 117, 431
AccIII TCCGGA 1 cut(s) 282
AciI CCGC 8 cut(s) 84, 115, 150, 262, 303, 326, 498, 827
AclWI GGATC 1 cut(s) 1109
AcoI YGGCCR 3 cut(s) 112, 229, 313
AcsI RAATTY 1 cut(s) 1480
AcvI CACGTG 1 cut(s) 1140
AdeI CACNNNGTG 1 cut(s) 927
AfaI GTAC 9 cut(s) 70, 239, 275, 533, 593, 821, 943, 981, 1136
AfiI CCNNNNNNNGG 5 cut(s) 134, 721, 927, 938, 1031
AflIII ACRYGT 1 cut(s) 1137
AgsI TTSAA 7 cut(s) 550, 721, 772, 834, 889, 1306, 1459
AjnI CCWGG 4 cut(s) 183, 252, 519, 1474
AluBI AGCT 4 cut(s) 321, 567, 585, 1383
AluI AGCT 4 cut(s) 321, 567, 585, 1383
Alw21I GWGCWC 1 cut(s) 323
Alw26I GTCTC 2 cut(s) 1180, 1221
AlwI GGATC 1 cut(s) 1109
Ama87I CYCGRG 1 cut(s) 1047
Aor13HI TCCGGA 1 cut(s) 282
AoxI GGCC 8 cut(s) 112, 181, 229, 313, 363, 776, 929, 1198
ApeKI GCWGC 1 cut(s) 264
ApoI RAATTY 1 cut(s) 1480
AseI ATTAAT 3 cut(s) 663, 861, 996
Asp700I GAANNNNTTC 2 cut(s) 123, 725
AspLEI GCGC 3 cut(s) 89, 269, 433
AspS9I GGNCC 8 cut(s) 187, 280, 285, 363, 599, 986, 1198, 1280
AsuHPI GGTGA 2 cut(s) 751, 1124
AsuII TTCGAA 1 cut(s) 729
AvaI CYCGRG 1 cut(s) 1047
AvaII GGWCC 6 cut(s) 187, 280, 285, 599, 986, 1280
BanI GGYRCC 1 cut(s) 353
BanII GRGCYC 1 cut(s) 323
BarI GAAGNNNNNNTAC 4 cut(s) 227, 259, 1027, 1059
BbrPI CACGTG 1 cut(s) 1140
BbsI GAAGAC 2 cut(s) 1115, 1338
Bbv12I GWGCWC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 276
BccI CCATC 2 cut(s) 53, 611
BceAI ACGGC 6 cut(s) 127, 316, 328, 350, 367, 412
BciT130I CCWGG 4 cut(s) 185, 254, 521, 1476
BclI TGATCA 2 cut(s) 75, 622
BcoDI GTCTC 2 cut(s) 1180, 1221
BfaI CTAG 2 cut(s) 576, 972
BfoI RGCGCY 1 cut(s) 270
BisI GCNGC 4 cut(s) 85, 115, 265, 326
BlsI GCNGC 4 cut(s) 86, 116, 266, 327
BmcAI AGTACT 2 cut(s) 239, 981
Bme1390I CCNGG 4 cut(s) 185, 254, 521, 1476
Bme18I GGWCC 6 cut(s) 187, 280, 285, 599, 986, 1280
BmeT110I CYCGRG 1 cut(s) 1047
BmgT120I GGNCC 8 cut(s) 187, 280, 285, 363, 599, 986, 1198, 1280
BmiI GGNNCC 5 cut(s) 157, 189, 355, 601, 645
BmrFI CCNGG 4 cut(s) 185, 254, 521, 1476
BmsI GCATC 1 cut(s) 787
BpiI GAAGAC 2 cut(s) 1115, 1338
BpmI CTGGAG 4 cut(s) 503, 1023, 1182, 1458
Bpu14I TTCGAA 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 655, 1106
BsaAI YACGTR 2 cut(s) 471, 1140
BsaI GGTCTC 1 cut(s) 1180
BsaJI CCNNGG 2 cut(s) 381, 1027
BsaWI WCCGGW 1 cut(s) 282
BsaXI ACNNNNNCTCC 2 cut(s) 1155, 1185
Bsc4I CCNNNNNNNGG 5 cut(s) 134, 721, 927, 938, 1031
Bse118I RCCGGY 3 cut(s) 227, 328, 787
Bse1I ACTGG 5 cut(s) 751, 1109, 1317, 1423, 1447
Bse3DI GCAATG 3 cut(s) 222, 682, 810
BseAI TCCGGA 1 cut(s) 282
BseBI CCWGG 4 cut(s) 185, 254, 521, 1476
BseDI CCNNGG 2 cut(s) 381, 1027
BseGI GGATG 1 cut(s) 802
BseLI CCNNNNNNNGG 5 cut(s) 134, 721, 927, 938, 1031
BseMI GCAATG 3 cut(s) 222, 682, 810
BseMII CTCAG 2 cut(s) 577, 1309
BseNI ACTGG 5 cut(s) 751, 1109, 1317, 1423, 1447
BseRI GAGGAG 5 cut(s) 11, 14, 17, 20, 29
BseX3I CGGCCG 3 cut(s) 112, 229, 313
BseXI GCAGC 1 cut(s) 276
BsgI GTGCAG 1 cut(s) 1307
Bsh1236I CGCG 2 cut(s) 117, 431
Bsh1285I CGRYCG 5 cut(s) 115, 212, 232, 316, 765
BshFI GGCC 8 cut(s) 114, 183, 231, 315, 365, 778, 931, 1200
BshNI GGYRCC 1 cut(s) 353
BsiEI CGRYCG 5 cut(s) 115, 212, 232, 316, 765
BsiHKAI GWGCWC 1 cut(s) 323
BsiHKCI CYCGRG 1 cut(s) 1047
BsiSI CCGG 8 cut(s) 135, 147, 228, 232, 283, 316, 329, 788
BslFI GGGAC 2 cut(s) 173, 739
BslI CCNNNNNNNGG 5 cut(s) 134, 721, 927, 938, 1031
BsmAI GTCTC 2 cut(s) 1180, 1221
BsmFI GGGAC 2 cut(s) 173, 739
BsnI GGCC 8 cut(s) 114, 183, 231, 315, 365, 778, 931, 1200
Bso31I GGTCTC 1 cut(s) 1180
BsoBI CYCGRG 1 cut(s) 1047
Bsp119I TTCGAA 1 cut(s) 729
Bsp1286I GDGCHC 1 cut(s) 323
Bsp13I TCCGGA 1 cut(s) 282
Bsp1407I TGTACA 1 cut(s) 531
Bsp143I GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
BspACI CCGC 8 cut(s) 84, 115, 150, 262, 303, 326, 498, 827
BspANI GGCC 8 cut(s) 114, 183, 231, 315, 365, 778, 931, 1200
BspCNI CTCAG 2 cut(s) 576, 1308
BspEI TCCGGA 1 cut(s) 282
BspFNI CGCG 2 cut(s) 117, 431
BspLI GGNNCC 5 cut(s) 157, 189, 355, 601, 645
BspPI GGATC 1 cut(s) 1109
BspT104I TTCGAA 1 cut(s) 729
BspT107I GGYRCC 1 cut(s) 353
BspTNI GGTCTC 1 cut(s) 1180
BsrDI GCAATG 3 cut(s) 222, 682, 810
BsrFI RCCGGY 3 cut(s) 227, 328, 787
BsrGI TGTACA 1 cut(s) 531
BsrI ACTGG 5 cut(s) 751, 1109, 1317, 1423, 1447
BssAI RCCGGY 3 cut(s) 227, 328, 787
BssECI CCNNGG 2 cut(s) 381, 1027
BssMI GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
BssNAI GTATAC 1 cut(s) 474
Bst1107I GTATAC 1 cut(s) 474
Bst2UI CCWGG 4 cut(s) 185, 254, 521, 1476
Bst4CI ACNGT 2 cut(s) 819, 1215
BstAPI GCANNNNNTGC 1 cut(s) 1155
BstAUI TGTACA 1 cut(s) 531
BstBAI YACGTR 2 cut(s) 471, 1140
BstBI TTCGAA 1 cut(s) 729
BstC8I GCNNGC 3 cut(s) 229, 323, 1385
BstDEI CTNAG 3 cut(s) 563, 1295, 1463
BstF5I GGATG 1 cut(s) 802
BstFNI CGCG 2 cut(s) 117, 431
BstH2I RGCGCY 1 cut(s) 270
BstHHI GCGC 3 cut(s) 89, 269, 433
BstKTI GATC 8 cut(s) 78, 212, 345, 625, 634, 765, 1117, 1294
BstMAI GTCTC 2 cut(s) 1180, 1221
BstMBI GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
BstMCI CGRYCG 5 cut(s) 115, 212, 232, 316, 765
BstMWI GCNNNNNNNGC 3 cut(s) 784, 1007, 1155
BstNI CCWGG 4 cut(s) 185, 254, 521, 1476
BstNSI RCATGY 2 cut(s) 403, 1158
BstSCI CCNGG 4 cut(s) 183, 252, 519, 1474
BstSNI TACGTA 1 cut(s) 471
BstUI CGCG 2 cut(s) 117, 431
BstV1I GCAGC 1 cut(s) 276
BstV2I GAAGAC 2 cut(s) 1115, 1338
BstXI CCANNNNNNTGG 2 cut(s) 277, 1104
BstZ17I GTATAC 1 cut(s) 474
BstZI CGGCCG 3 cut(s) 112, 229, 313
BsuRI GGCC 8 cut(s) 114, 183, 231, 315, 365, 778, 931, 1200
BtgZI GCGATG 1 cut(s) 1347
BtsCI GGATG 1 cut(s) 802
BtsI GCAGTG 2 cut(s) 96, 1319
BtsIMutI CAGTG 6 cut(s) 96, 815, 1211, 1319, 1351, 1416
Cac8I GCNNGC 3 cut(s) 229, 323, 1385
CfoI GCGC 3 cut(s) 89, 269, 433
Cfr10I RCCGGY 3 cut(s) 227, 328, 787
Cfr13I GGNCC 8 cut(s) 187, 280, 285, 363, 599, 986, 1198, 1280
CseI GACGC 2 cut(s) 439, 445
Csp6I GTAC 9 cut(s) 69, 238, 274, 532, 592, 820, 942, 980, 1135
CviAII CATG 4 cut(s) 277, 400, 445, 1155
CviQI GTAC 9 cut(s) 69, 238, 274, 532, 592, 820, 942, 980, 1135
DdeI CTNAG 3 cut(s) 563, 1295, 1463
DpnI GATC 8 cut(s) 77, 211, 344, 624, 633, 764, 1116, 1293
DpnII GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
DraI TTTAAA 1 cut(s) 1182
DraIII CACNNNGTG 1 cut(s) 927
DrdI GACNNNNNNGTC 2 cut(s) 751, 1243
DseDI GACNNNNNNGTC 2 cut(s) 751, 1243
EaeI YGGCCR 3 cut(s) 112, 229, 313
EagI CGGCCG 3 cut(s) 112, 229, 313
EciI GGCGGA 1 cut(s) 318
Ecl136II GAGCTC 1 cut(s) 321
EclXI CGGCCG 3 cut(s) 112, 229, 313
Eco105I TACGTA 1 cut(s) 471
Eco147I AGGCCT 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 323
Eco31I GGTCTC 1 cut(s) 1180
Eco47I GGWCC 6 cut(s) 187, 280, 285, 599, 986, 1280
Eco52I CGGCCG 3 cut(s) 112, 229, 313
Eco53kI GAGCTC 1 cut(s) 321
Eco72I CACGTG 1 cut(s) 1140
Eco88I CYCGRG 1 cut(s) 1047
EcoICRI GAGCTC 1 cut(s) 321
EcoO109I RGGNCCY 1 cut(s) 1280
EcoRII CCWGG 4 cut(s) 183, 252, 519, 1474
EcoT38I GRGCYC 1 cut(s) 323
FaeI CATG 4 cut(s) 280, 403, 448, 1158
FalI AAGNNNNNCTT 4 cut(s) 891, 923, 1068, 1100
FaqI GGGAC 2 cut(s) 173, 739
FatI CATG 4 cut(s) 276, 399, 444, 1154
FbaI TGATCA 2 cut(s) 75, 622
FblI GTMKAC 2 cut(s) 258, 473
Fnu4HI GCNGC 4 cut(s) 85, 115, 265, 326
FokI GGATG 1 cut(s) 809
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 4 cut(s) 85, 115, 265, 326
FspBI CTAG 2 cut(s) 576, 972
GlaI GCGC 3 cut(s) 88, 268, 432
GluI GCNGC 4 cut(s) 85, 115, 265, 326
GsuI CTGGAG 4 cut(s) 503, 1023, 1182, 1458
HaeII RGCGCY 1 cut(s) 270
HaeIII GGCC 8 cut(s) 114, 183, 231, 315, 365, 778, 931, 1200
HapII CCGG 8 cut(s) 135, 147, 228, 232, 283, 316, 329, 788
HgaI GACGC 2 cut(s) 439, 445
HhaI GCGC 3 cut(s) 89, 269, 433
Hin1II CATG 4 cut(s) 280, 403, 448, 1158
Hin6I GCGC 3 cut(s) 87, 267, 431
HinP1I GCGC 3 cut(s) 87, 267, 431
HincII GTYRAC 2 cut(s) 373, 394
HindII GTYRAC 2 cut(s) 373, 394
HpaII CCGG 8 cut(s) 135, 147, 228, 232, 283, 316, 329, 788
HphI GGTGA 2 cut(s) 751, 1124
Hpy166II GTNNAC 8 cut(s) 259, 373, 394, 474, 1137, 1143, 1211, 1391
Hpy188I TCNGA 5 cut(s) 209, 484, 1031, 1221, 1249
Hpy8I GTNNAC 8 cut(s) 259, 373, 394, 474, 1137, 1143, 1211, 1391
Hpy99I CGWCG 3 cut(s) 353, 371, 461
HpyAV CCTTC 4 cut(s) 367, 715, 975, 999
HpyCH4III ACNGT 2 cut(s) 819, 1215
HpyCH4IV ACGT 4 cut(s) 249, 390, 470, 1139
HpyCH4V TGCA 5 cut(s) 680, 800, 815, 1241, 1324
HpyF10VI GCNNNNNNNGC 3 cut(s) 784, 1007, 1155
HpyF3I CTNAG 3 cut(s) 563, 1295, 1463
HpySE526I ACGT 4 cut(s) 249, 390, 470, 1139
Hsp92II CATG 4 cut(s) 280, 403, 448, 1158
HspAI GCGC 3 cut(s) 87, 267, 431
Kpn2I TCCGGA 1 cut(s) 282
KroI GCCGGC 1 cut(s) 227
KroNI GCCGGC 1 cut(s) 229
Ksp22I TGATCA 2 cut(s) 75, 622
Kzo9I GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
LmnI GCTCC 2 cut(s) 318, 643
Lsp1109I GCAGC 1 cut(s) 276
LweI GCATC 1 cut(s) 787
MaeI CTAG 2 cut(s) 576, 972
MaeII ACGT 4 cut(s) 249, 390, 470, 1139
MaeIII GTNAC 2 cut(s) 739, 892
MalI GATC 8 cut(s) 77, 211, 344, 624, 633, 764, 1116, 1293
MboI GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
MboII GAAGA 5 cut(s) 960, 1047, 1115, 1318, 1343
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 5 cut(s) 105, 772, 1270, 1340, 1480
MlyI GAGTC 6 cut(s) 125, 473, 518, 555, 662, 1090
MmeI TCCRAC 1 cut(s) 507
MroI TCCGGA 1 cut(s) 282
MroNI GCCGGC 1 cut(s) 227
MroXI GAANNNNTTC 2 cut(s) 123, 725
MseI TTAA 4 cut(s) 663, 861, 996, 1181
MspI CCGG 8 cut(s) 135, 147, 228, 232, 283, 316, 329, 788
MspR9I CCNGG 4 cut(s) 185, 254, 521, 1476
MvaI CCWGG 4 cut(s) 185, 254, 521, 1476
MvnI CGCG 2 cut(s) 117, 431
MwoI GCNNNNNNNGC 3 cut(s) 784, 1007, 1155
NaeI GCCGGC 1 cut(s) 229
NdeII GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
NgoMIV GCCGGC 1 cut(s) 227
NlaIII CATG 4 cut(s) 280, 403, 448, 1158
NlaIV GGNNCC 5 cut(s) 157, 189, 355, 601, 645
NmuCI GTSAC 2 cut(s) 739, 892
NspI RCATGY 2 cut(s) 403, 1158
NspV TTCGAA 1 cut(s) 729
PaeR7I CTCGAG 1 cut(s) 1047
PceI AGGCCT 1 cut(s) 183
PdiI GCCGGC 1 cut(s) 229
PdmI GAANNNNTTC 2 cut(s) 123, 725
PfeI GAWTC 6 cut(s) 98, 306, 514, 636, 830, 1459
PflFI GACNNNGTC 1 cut(s) 389
PflMI CCANNNNNTGG 1 cut(s) 927
PfoI TCCNGGA 2 cut(s) 519, 1474
PkrI GCNGC 4 cut(s) 86, 116, 266, 327
Ple19I CGATCG 2 cut(s) 212, 765
PleI GAGTC 6 cut(s) 125, 473, 518, 555, 662, 1089
PmaCI CACGTG 1 cut(s) 1140
PmlI CACGTG 1 cut(s) 1140
PpsI GAGTC 6 cut(s) 125, 473, 518, 555, 662, 1089
Ppu21I YACGTR 2 cut(s) 471, 1140
PpuMI RGGWCCY 1 cut(s) 1280
PshBI ATTAAT 3 cut(s) 663, 861, 996
Psp124BI GAGCTC 1 cut(s) 323
Psp5II RGGWCCY 1 cut(s) 1280
Psp6I CCWGG 4 cut(s) 183, 252, 519, 1474
PspCI CACGTG 1 cut(s) 1140
PspGI CCWGG 4 cut(s) 183, 252, 519, 1474
PspN4I GGNNCC 5 cut(s) 157, 189, 355, 601, 645
PspPI GGNCC 8 cut(s) 187, 280, 285, 363, 599, 986, 1198, 1280
PspPPI RGGWCCY 1 cut(s) 1280
PsrI GAACNNNNNNTAC 2 cut(s) 1383, 1415
PsyI GACNNNGTC 1 cut(s) 389
PvuI CGATCG 2 cut(s) 212, 765
RsaI GTAC 9 cut(s) 70, 239, 275, 533, 593, 821, 943, 981, 1136
RsaNI GTAC 9 cut(s) 69, 238, 274, 532, 592, 820, 942, 980, 1135
SacI GAGCTC 1 cut(s) 323
SaqAI TTAA 4 cut(s) 663, 861, 996, 1181
SatI GCNGC 4 cut(s) 85, 115, 265, 326
Sau3AI GATC 8 cut(s) 75, 209, 342, 622, 631, 762, 1114, 1291
Sau96I GGNCC 8 cut(s) 187, 280, 285, 363, 599, 986, 1198, 1280
ScaI AGTACT 2 cut(s) 239, 981
SchI GAGTC 6 cut(s) 125, 473, 518, 555, 662, 1090
ScrFI CCNGG 4 cut(s) 185, 254, 521, 1476
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 1 cut(s) 787
Sfr274I CTCGAG 1 cut(s) 1047
SfuI TTCGAA 1 cut(s) 729
SgrAI CRCCGGYG 1 cut(s) 328
SinI GGWCC 6 cut(s) 187, 280, 285, 599, 986, 1280
SlaI CTCGAG 1 cut(s) 1047
SmlI CTYRAG 3 cut(s) 670, 1047, 1085
SmoI CTYRAG 3 cut(s) 670, 1047, 1085
SnaBI TACGTA 1 cut(s) 471
Sse9I AATT 5 cut(s) 105, 772, 1270, 1340, 1480
SseBI AGGCCT 1 cut(s) 183
SsiI CCGC 8 cut(s) 84, 115, 150, 262, 303, 326, 498, 827
SspI AATATT 1 cut(s) 952
SspMI CTAG 2 cut(s) 576, 972
SstI GAGCTC 1 cut(s) 323
StuI AGGCCT 1 cut(s) 183
StyD4I CCNGG 4 cut(s) 183, 252, 519, 1474
TaaI ACNGT 2 cut(s) 819, 1215
TaiI ACGT 4 cut(s) 252, 393, 473, 1142
TaqI TCGA 8 cut(s) 96, 336, 348, 634, 729, 765, 992, 1048
TasI AATT 5 cut(s) 105, 772, 1270, 1340, 1480
TatI WGTACW 6 cut(s) 237, 273, 531, 819, 979, 1134
TauI GCSGC 3 cut(s) 87, 117, 328
TfiI GAWTC 6 cut(s) 98, 306, 514, 636, 830, 1459
Tru1I TTAA 4 cut(s) 663, 861, 996, 1181
Tru9I TTAA 4 cut(s) 663, 861, 996, 1181
TscAI CASTG 6 cut(s) 96, 822, 1218, 1326, 1351, 1423
TseFI GTSAC 2 cut(s) 739, 892
TseI GCWGC 1 cut(s) 264
Tsp45I GTSAC 2 cut(s) 739, 892
TspDTI ATGAA 3 cut(s) 130, 847, 1290
TspGWI ACGGA 2 cut(s) 491, 557
TspRI CASTG 6 cut(s) 96, 822, 1218, 1326, 1351, 1423
Tth111I GACNNNGTC 1 cut(s) 389
Van91I CCANNNNNTGG 1 cut(s) 927
VpaK11BI GGWCC 6 cut(s) 187, 280, 285, 599, 986, 1280
VspI ATTAAT 3 cut(s) 663, 861, 996
XapI RAATTY 1 cut(s) 1480
XceI RCATGY 2 cut(s) 403, 1158
XhoI CTCGAG 1 cut(s) 1047
XmiI GTMKAC 2 cut(s) 258, 473
XmnI GAANNNNTTC 2 cut(s) 123, 725
XspI CTAG 2 cut(s) 576, 972
ZrmI AGTACT 2 cut(s) 239, 981
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.