pycom13g16460
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0024688
Physical Location & Seq
Forward (+)
12259414 .. 12259842
429 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g16460.1

Sequence Viewer

Length: 429 bp
ATGGCTAGCCTTCATGTACTCAGACTGATGCCTGAACCAACTGCCATCGCACTGTTATATGCACAAAAAATTTCAAATCAGAATGTAAACACCGGATCTGAGAAGATTGCTCTAATCTTCAACATGGGTGCTGGATACTGCGATGTAGCTGTTACTGCTACAGCTGGAGGAGTTTCCCAAATAAAAGCCAGCCAACATTTAGCAGGATCTGCCATTGGAGGGGAAGACTTGCTTCAGAATATGATGCGTTATCTGTTGCCAGATTCTGAGACTCTTTTAACTAGCCACGGACTTGATGACATAAAATCAGCGGGCATTCTTCGAGTTGCAACACAGGAAGCAATCCACAATCTCTCCTCCCAAACAAGTGTCCAGATTGATGTTGACTTGGGAAATGGAACAAATATATGTAAGGTTGTGGATCGATAA

Protein Analysis

143

Amino Acids

14.99

Weight (kDa)

5.4

Isoelectric Point (pI)

30.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 2 - 129 4.3e-12 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0024688)

Species Orthologous Gene IDs
pyrus_communis pycom13g16460
rosa_chinensis RchiOBHm_Chr6g0290301
rosa_samantha Rh6CG335100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 311
AclWI GGATC 2 cut(s) 103, 214
AcsI RAATTY 1 cut(s) 69
AcuI CTGAAG 1 cut(s) 218
AfaI GTAC 1 cut(s) 18
AfiI CCNNNNNNNGG 1 cut(s) 219
AgsI TTSAA 2 cut(s) 75, 121
AluBI AGCT 2 cut(s) 149, 164
AluI AGCT 2 cut(s) 149, 164
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 2 cut(s) 103, 214
AlwNI CAGNNNCTG 2 cut(s) 209, 266
ApoI RAATTY 1 cut(s) 69
AsuNHI GCTAGC 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 231
BccI CCATC 1 cut(s) 53
BciVI GTATCC 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 263
BfaI CTAG 2 cut(s) 6, 282
BfmI CTRYAG 1 cut(s) 159
BfuI GTATCC 1 cut(s) 128
BmsI GCATC 2 cut(s) 18, 234
BmtI GCTAGC 1 cut(s) 9
BpiI GAAGAC 1 cut(s) 231
BpmI CTGGAG 1 cut(s) 186
Bsa29I ATCGAT 1 cut(s) 424
BsaJI CCNNGG 1 cut(s) 286
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 2 cut(s) 338, 368
Bsc4I CCNNNNNNNGG 1 cut(s) 219
BseCI ATCGAT 1 cut(s) 424
BseDI CCNNGG 1 cut(s) 286
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 3 cut(s) 34, 90, 258
BseRI GAGGAG 2 cut(s) 183, 346
BshVI ATCGAT 1 cut(s) 424
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 1 cut(s) 219
BsmAI GTCTC 1 cut(s) 263
BsmI GAATGC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 95, 206, 421
BspACI CCGC 1 cut(s) 311
BspCNI CTCAG 3 cut(s) 33, 91, 259
BspDI ATCGAT 1 cut(s) 424
BspOI GCTAGC 1 cut(s) 9
BspPI GGATC 2 cut(s) 103, 214
BssECI CCNNGG 1 cut(s) 286
BssMI GATC 3 cut(s) 95, 206, 421
Bst4CI ACNGT 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 3 cut(s) 7, 190, 313
BstDEI CTNAG 3 cut(s) 20, 99, 267
BstDSI CCRYGG 1 cut(s) 286
BstKTI GATC 3 cut(s) 98, 209, 424
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 3 cut(s) 95, 206, 421
BstMWI GCNNNNNNNGC 2 cut(s) 155, 209
BstSFI CTRYAG 1 cut(s) 159
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 2 cut(s) 95, 206
BstYI RGATCY 2 cut(s) 95, 206
Bsu15I ATCGAT 1 cut(s) 424
BsuI GTATCC 1 cut(s) 128
BsuTUI ATCGAT 1 cut(s) 424
BtgI CCRYGG 1 cut(s) 286
BtgZI GCGATG 2 cut(s) 31, 156
BtsIMutI CAGTG 1 cut(s) 50
Cac8I GCNNGC 3 cut(s) 7, 190, 313
CaiI CAGNNNCTG 2 cut(s) 209, 266
ClaI ATCGAT 1 cut(s) 424
Csp6I GTAC 1 cut(s) 17
CviAII CATG 2 cut(s) 14, 124
CviJI RGCY 7 cut(s) 5, 9, 149, 164, 188, 192, 285
CviKI_1 RGCY 7 cut(s) 5, 9, 149, 164, 188, 192, 285
CviQI GTAC 1 cut(s) 17
DdeI CTNAG 3 cut(s) 20, 99, 267
DpnI GATC 3 cut(s) 97, 208, 423
DpnII GATC 3 cut(s) 95, 206, 421
Eco57I CTGAAG 1 cut(s) 218
FaeI CATG 2 cut(s) 17, 127
FaiI YATR 8 cut(s) 15, 58, 60, 125, 242, 302, 407, 409
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FatI CATG 2 cut(s) 13, 123
FauI CCCGC 1 cut(s) 304
FspBI CTAG 2 cut(s) 6, 282
GsuI CTGGAG 1 cut(s) 186
HapII CCGG 1 cut(s) 93
Hin1II CATG 2 cut(s) 17, 127
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 2 cut(s) 263, 271
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 2 cut(s) 88, 385
Hpy188I TCNGA 5 cut(s) 23, 81, 100, 237, 268
Hpy188III TCNNGA 1 cut(s) 373
Hpy8I GTNNAC 2 cut(s) 88, 385
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4V TGCA 2 cut(s) 62, 329
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 209
HpyF3I CTNAG 3 cut(s) 20, 99, 267
Hsp92II CATG 2 cut(s) 17, 127
Kzo9I GATC 3 cut(s) 95, 206, 421
LpnPI CCDG 9 cut(s) 45, 106, 117, 150, 189, 202, 273, 320, 386
LweI GCATC 2 cut(s) 18, 234
MaeI CTAG 2 cut(s) 6, 282
MaeIII GTNAC 1 cut(s) 151
MalI GATC 3 cut(s) 97, 208, 423
MboI GATC 3 cut(s) 95, 206, 421
MboII GAAGA 4 cut(s) 109, 115, 236, 311
MflI RGATCY 2 cut(s) 95, 206
MluCI AATT 1 cut(s) 69
MlyI GAGTC 1 cut(s) 265
MnlI CCTC 3 cut(s) 161, 212, 367
MseI TTAA 1 cut(s) 278
MspA1I CMGCKG 2 cut(s) 164, 311
MspI CCGG 1 cut(s) 93
Mva1269I GAATGC 1 cut(s) 315
MwoI GCNNNNNNNGC 2 cut(s) 155, 209
NdeII GATC 3 cut(s) 95, 206, 421
NheI GCTAGC 1 cut(s) 5
NlaIII CATG 2 cut(s) 17, 127
PctI GAATGC 1 cut(s) 315
PfeI GAWTC 1 cut(s) 263
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PstNI CAGNNNCTG 2 cut(s) 209, 266
PsuI RGATCY 2 cut(s) 95, 206
PvuII CAGCTG 1 cut(s) 164
RsaI GTAC 1 cut(s) 18
RsaNI GTAC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 278
Sau3AI GATC 3 cut(s) 95, 206, 421
SchI GAGTC 1 cut(s) 265
SetI ASST 3 cut(s) 151, 166, 417
SfaNI GCATC 2 cut(s) 18, 234
SfcI CTRYAG 1 cut(s) 159
Sse9I AATT 1 cut(s) 69
SsiI CCGC 1 cut(s) 311
SspMI CTAG 2 cut(s) 6, 282
TaaI ACNGT 1 cut(s) 54
TaqI TCGA 2 cut(s) 322, 424
TasI AATT 1 cut(s) 69
TatI WGTACW 1 cut(s) 16
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 1 cut(s) 278
Tru9I TTAA 1 cut(s) 278
TscAI CASTG 1 cut(s) 57
TspGWI ACGGA 1 cut(s) 303
TspRI CASTG 1 cut(s) 57
XapI RAATTY 1 cut(s) 69
XspI CTAG 2 cut(s) 6, 282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.