Rh6CG335100
HSP70 Family

Heat shock 70 kDa protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0024688
Physical Location & Seq
Forward (+)
52359409 .. 52359888
480 bp
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UTR
Exon/CDS
Intron
Rh6CG335100.1

Sequence Viewer

Length: 480 bp
ATGCCAGAACCAACTGCAGTGGCATTGTTATATACCTTATATACAAAACAAAGGGGATTTAAGTCAACAGATAGGAATGTTCTAATCTTCAATATGGGTGCAGGATACTGTGATGTTGCCATAAGTGTGACAGCTGAAGAGGCACCACGAGGAATTTCTGAAATAATTGCCTTAACAGGAAGTGCCATTGGAGGAGAAGACTTGCTTCAGAATATGATGCGTCATCTGTTGCCAAATGCAGAGAATCTTTTGACAAGTCATGGAGTTGACGAAATCAAATCAAAGGGGTTACTTCGAGTTGCAACACAGAATGCAATCCAGAGTCTCTCCTCCCAAAATTCTGTTCAAATTGATGTTGACTTGGGAAATGGGATGAAAATCTGCAAGGTAGTGGACCGAGAAGAATTTGAGGAAGTTAATCGTGCAGTATTTAATAAGTGTGTTAGCCTCATATCCAGTGTTTGCATGATGCAAAGGTAA

Protein Analysis

159

Amino Acids

17.36

Weight (kDa)

5.39

Isoelectric Point (pI)

30.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP70 PF00012 3 - 153 1.7e-12 Hsp70 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024688)

Species Orthologous Gene IDs
pyrus_communis pycom13g16460
rosa_chinensis RchiOBHm_Chr6g0290301
rosa_samantha Rh6CG335100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 142
AcsI RAATTY 3 cut(s) 153, 337, 404
AcuI CTGAAG 2 cut(s) 156, 191
AgsI TTSAA 2 cut(s) 91, 347
AjuI GAANNNNNNNTTGG 2 cut(s) 327, 359
AluBI AGCT 1 cut(s) 134
AluI AGCT 1 cut(s) 134
Alw26I GTCTC 1 cut(s) 329
ApoI RAATTY 3 cut(s) 153, 337, 404
AspS9I GGNCC 1 cut(s) 394
AvaII GGWCC 1 cut(s) 394
BanI GGYRCC 1 cut(s) 142
BauI CACGAG 1 cut(s) 147
BbsI GAAGAC 1 cut(s) 204
BciVI GTATCC 1 cut(s) 98
BcoDI GTCTC 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 15
BfuI GTATCC 1 cut(s) 98
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 1 cut(s) 394
BmiI GGNNCC 1 cut(s) 144
BmsI GCATC 2 cut(s) 207, 459
BpiI GAAGAC 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 377
Bse1I ACTGG 1 cut(s) 456
Bse8I GATNNNNATC 1 cut(s) 377
BseGI GGATG 1 cut(s) 378
BseJI GATNNNNATC 1 cut(s) 377
BseNI ACTGG 1 cut(s) 456
BseRI GAGGAG 2 cut(s) 207, 319
BsgI GTGCAG 2 cut(s) 120, 444
BshNI GGYRCC 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 316
BspLI GGNNCC 1 cut(s) 144
BspMAI CTGCAG 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 142
BsrI ACTGG 1 cut(s) 456
BssSI CACGAG 1 cut(s) 147
Bst2BI CACGAG 1 cut(s) 147
Bst4CI ACNGT 1 cut(s) 110
Bst6I CTCTTC 1 cut(s) 132
BstF5I GGATG 1 cut(s) 378
BstMAI GTCTC 1 cut(s) 329
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstSFI CTRYAG 1 cut(s) 15
BstV2I GAAGAC 1 cut(s) 204
BsuI GTATCC 1 cut(s) 98
BtsCI GGATG 1 cut(s) 378
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 2 cut(s) 24, 463
Cfr13I GGNCC 1 cut(s) 394
CseI GACGC 1 cut(s) 209
CspCI CAANNNNNGTGG 1 cut(s) 35
CviAII CATG 2 cut(s) 260, 466
CviJI RGCY 2 cut(s) 134, 447
CviKI_1 RGCY 2 cut(s) 134, 447
Eam1104I CTCTTC 1 cut(s) 132
EarI CTCTTC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 394
Eco57I CTGAAG 2 cut(s) 156, 191
FaeI CATG 2 cut(s) 263, 469
FalI AAGNNNNNCTT 2 cut(s) 189, 221
FatI CATG 2 cut(s) 259, 465
FokI GGATG 1 cut(s) 385
HgaI GACGC 1 cut(s) 209
Hin1II CATG 2 cut(s) 263, 469
HincII GTYRAC 3 cut(s) 66, 268, 358
HindII GTYRAC 3 cut(s) 66, 268, 358
HinfI GANTC 2 cut(s) 244, 322
Hpy166II GTNNAC 4 cut(s) 66, 268, 358, 394
Hpy188I TCNGA 2 cut(s) 160, 210
Hpy188III TCNNGA 1 cut(s) 319
Hpy8I GTNNAC 4 cut(s) 66, 268, 358, 394
HpyCH4III ACNGT 1 cut(s) 110
HpyCH4V TGCA 9 cut(s) 17, 101, 239, 302, 314, 384, 425, 465, 472
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
Hsp92II CATG 2 cut(s) 263, 469
LpnPI CCDG 5 cut(s) 18, 87, 162, 332, 469
LweI GCATC 2 cut(s) 207, 459
MaeIII GTNAC 2 cut(s) 127, 288
MboII GAAGA 4 cut(s) 79, 149, 209, 413
MluCI AATT 5 cut(s) 153, 165, 337, 348, 404
MlyI GAGTC 1 cut(s) 331
MnlI CCTC 6 cut(s) 133, 143, 185, 340, 403, 458
MseI TTAA 4 cut(s) 60, 173, 417, 432
MslI CAYNNNNRTG 1 cut(s) 125
MspA1I CMGCKG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 316
MwoI GCNNNNNNNGC 1 cut(s) 140
NlaIII CATG 2 cut(s) 263, 469
NlaIV GGNNCC 1 cut(s) 144
NmuCI GTSAC 1 cut(s) 127
PctI GAATGC 1 cut(s) 316
PfeI GAWTC 1 cut(s) 244
PleI GAGTC 1 cut(s) 330
PpsI GAGTC 1 cut(s) 330
PspN4I GGNNCC 1 cut(s) 144
PspPI GGNCC 1 cut(s) 394
PstI CTGCAG 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 134
RseI CAYNNNNRTG 1 cut(s) 125
SaqAI TTAA 4 cut(s) 60, 173, 417, 432
Sau96I GGNCC 1 cut(s) 394
SchI GAGTC 1 cut(s) 331
SetI ASST 4 cut(s) 38, 136, 390, 479
SfaNI GCATC 2 cut(s) 207, 459
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 1 cut(s) 394
SmiMI CAYNNNNRTG 1 cut(s) 125
Sse9I AATT 5 cut(s) 153, 165, 337, 348, 404
TaaI ACNGT 1 cut(s) 110
TaqI TCGA 1 cut(s) 295
TaqII GACCGA 1 cut(s) 411
TasI AATT 5 cut(s) 153, 165, 337, 348, 404
TfiI GAWTC 1 cut(s) 244
Tru1I TTAA 4 cut(s) 60, 173, 417, 432
Tru9I TTAA 4 cut(s) 60, 173, 417, 432
TscAI CASTG 2 cut(s) 24, 463
TseFI GTSAC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 389
TspRI CASTG 2 cut(s) 24, 463
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 3 cut(s) 153, 337, 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.