pycom13g28200

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0024071
Physical Location & Seq
Forward (+)
24163196 .. 24163504
309 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 309 bp
ATGGCACAATGTGACAAGAAAGATGTCCTTATTGTTGGTGGTGTTGGTTGCAATGAACGGTTGCAAGAGGTGATGAGAACCATGTGCTCTGAACGGGGTGGAAGGTTGTTTGCAACCGATGATAGGTATTGTATTGATAATGGGGCCATGATTGCTTATACTGGTCTTCTTGCTTTTGCCAATGGCACATCAACACCGCTGGACAAGTCGACTTTTACCCAACGGTTTCGCACTGATGATGTTGAAGCAGTCTGGAGAATAAAAGAGGAATCAGAAAAAGTAAATGGATTGATGGATGAGAGTACCTGA

Protein Analysis

103

Amino Acids

11.38

Weight (kDa)

4.78

Isoelectric Point (pI)

14.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TsaD PF00814 2 - 53 4.2e-11 tRNA N6-adenosine threonylcarbamoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0024071)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g16550
pyrus_communis pycom13g28200
rosa_rugosa Rorug01G0083400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 209
AciI CCGC 1 cut(s) 197
AdeI CACNNNGTG 1 cut(s) 11
AfaI GTAC 1 cut(s) 304
AfiI CCNNNNNNNGG 1 cut(s) 123
AgsI TTSAA 1 cut(s) 245
Alw21I GWGCWC 1 cut(s) 89
AoxI GGCC 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 144
AsuHPI GGTGA 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 89
BccI CCATC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 145
BpiI GAAGAC 1 cut(s) 158
BpmI CTGGAG 1 cut(s) 274
Bsc4I CCNNNNNNNGG 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 166
Bse3DI GCAATG 1 cut(s) 58
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 123
BseMI GCAATG 1 cut(s) 58
BseNI ACTGG 1 cut(s) 166
BshFI GGCC 1 cut(s) 146
BsiHKAI GWGCWC 1 cut(s) 89
BslI CCNNNNNNNGG 1 cut(s) 123
BsnI GGCC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 89
BspACI CCGC 1 cut(s) 197
BspANI GGCC 1 cut(s) 146
BspLI GGNNCC 1 cut(s) 145
BsrDI GCAATG 1 cut(s) 58
BsrI ACTGG 1 cut(s) 166
Bst4CI ACNGT 2 cut(s) 60, 225
BstF5I GGATG 1 cut(s) 301
BstMWI GCNNNNNNNGC 1 cut(s) 152
BstV2I GAAGAC 1 cut(s) 158
BsuRI GGCC 1 cut(s) 146
BtsCI GGATG 1 cut(s) 301
BtsIMutI CAGTG 1 cut(s) 231
Cfr13I GGNCC 1 cut(s) 144
Csp6I GTAC 1 cut(s) 303
CviAII CATG 2 cut(s) 82, 148
CviJI RGCY 1 cut(s) 146
CviKI_1 RGCY 1 cut(s) 146
CviQI GTAC 1 cut(s) 303
DraIII CACNNNGTG 1 cut(s) 11
FaeI CATG 2 cut(s) 85, 151
FaiI YATR 3 cut(s) 83, 149, 159
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FatI CATG 2 cut(s) 81, 147
FblI GTMKAC 1 cut(s) 209
GsuI CTGGAG 1 cut(s) 274
HaeIII GGCC 1 cut(s) 146
Hin1II CATG 2 cut(s) 85, 151
HincII GTYRAC 1 cut(s) 210
HindII GTYRAC 1 cut(s) 210
HinfI GANTC 1 cut(s) 269
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 2 cut(s) 91, 274
Hpy188III TCNNGA 1 cut(s) 253
Hpy8I GTNNAC 1 cut(s) 210
HpyAV CCTTC 1 cut(s) 96
HpyCH4III ACNGT 2 cut(s) 60, 225
HpyCH4V TGCA 3 cut(s) 51, 64, 113
HpyF10VI GCNNNNNNNGC 1 cut(s) 152
Hsp92II CATG 2 cut(s) 85, 151
LpnPI CCDG 3 cut(s) 147, 185, 238
MaeIII GTNAC 1 cut(s) 11
MboII GAAGA 1 cut(s) 158
MhlI GDGCHC 1 cut(s) 89
MnlI CCTC 2 cut(s) 61, 259
MspA1I CMGCKG 1 cut(s) 199
MwoI GCNNNNNNNGC 1 cut(s) 152
NlaIII CATG 2 cut(s) 85, 151
NlaIV GGNNCC 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 269
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 1 cut(s) 144
RsaI GTAC 1 cut(s) 304
RsaNI GTAC 1 cut(s) 303
SalI GTCGAC 1 cut(s) 208
Sau96I GGNCC 1 cut(s) 144
SduI GDGCHC 1 cut(s) 89
SetI ASST 4 cut(s) 72, 107, 128, 308
SsiI CCGC 1 cut(s) 197
TaaI ACNGT 2 cut(s) 60, 225
TaqI TCGA 1 cut(s) 209
TfiI GAWTC 1 cut(s) 269
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 69
TspRI CASTG 1 cut(s) 238
XmiI GTMKAC 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.