FvH4_3g16550

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014229
Physical Location & Seq
Forward (+)
10441198 .. 10444277
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16550.t2

Sequence Viewer

Length: 1062 bp
ATGAATTATTGTACAAAAATGAAGAAACAGATTGCTCTAGGTTTTGAGGGTTCAGCAAACAAGATTGGTGTCGGCGTTGTGACCTTAGATGGCACAATCTTGTCAAACCCACGTCACACATACATTACCCCTCCTGGCCAAGGTTTCCTTCCCCGTGAAACTGCACAACACCATCTCCAACACATTCTGCCGTTAGTTAAATCTGCTTTGGAAACTGCACAAGTAACCCTTCAAGAGATCGATTGCCTTTGTTACACCAAAGGTCCTGGAATGGGAGCGCCGCTACAAGTTGCTGCCATTGTTGTTAGGGTTCTTTCACAGCTGTGGAAGAAGCCCATTGTTGCAGTTAATCATTGTGTTGCGCATATCGAGATGGGAAGGATTGTGACTGGCGCGGTTGATCCTGTTGTGTTGTATGTGAGTGGGGGGAACACACAGGTGATTGCCTATAGTGAAGGGAGGTATCGAATCTTTGGAGAGACCATTGATATTGCTGTTGGAAATTGCTTGGATCGGTTTGCCAGGGTATTAGAACTCTCAAATGATCCAGCCCCGGGATATAACATTGAGCAGCTTGCAAAGAAAGGAGAACAGTTTATAGACCTTCCTTATGCCGTCAAAGGGATGGATGTGTCTTTTAGTGGAATTCTGAGCTACATTGAAGCCACTGCTGTGGAGAAACTAAAAAACGATGAATGCACCCCTGCAGACTTATGCTACTCACTTCAGGAAAATGTGTTTGCAATGCTTGTTGAGATAACAGAACGTGCAATGGCACATTGTGACAAGAGAGATGTACTTATTGTTGGTGGTGTGGGTTGCAATGAGCGCCTGCAAGAGATGATGAAAACCATGTGTGCCGAACGAGGTGGACGATTGTTTGCAACTGATGATAGGTATTGTATTGATAATGGGGCAATGATTGCTTATACTGGTCTTCTTGCTTTTGCTCATGGAACATCAACCCCATTGGAGGAATCGACTTTCACGCAACGGTTCCGGACTGATGAAGTGGAAGCAATCTGGAGAGTAAAAGAGGAGTCAGCAAAAGCGAACGGGTGA

Protein Analysis

354

Amino Acids

38.68

Weight (kDa)

5.69

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TsaD PF00814 38 - 310 1.2e-84 tRNA N6-adenosine threonylcarbamoyltransferase
HypF_C_2 PF22521 184 - 303 2.4e-06 Carbamoyltransferase, Kae1-like Domain, second subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22720 AT4G22720
fragaria_vesca FvH4_3g16550 FvH4_3g16550
malus_domestica MD17G1219400.v1.1
prunus_persica Prupe.1G196300_v2.0.a1
pyrus_communis pycom17g22410
rosa_chinensis RchiOBHm_Chr1g0331181
rosa_laevigata RLG00000029747
rosa_multiflora Rmu_sc0005711.1_g000004
rosa_roxburghii Rroxscaffold_4G00319280
rosa_rugosa Rorug01G0092100
rosa_samantha Rh1AG116100 Rh1BG087800 Rh1CG111600 Rh1DG120500
rosa_wichuraiana Rw1G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 363
AccII CGCG 1 cut(s) 395
AccIII TCCGGA 1 cut(s) 999
AciI CCGC 2 cut(s) 281, 395
AclWI GGATC 3 cut(s) 395, 519, 539
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 1 cut(s) 645
AcuI CTGAAG 1 cut(s) 710
AdeI CACNNNGTG 1 cut(s) 782
AfaI GTAC 2 cut(s) 13, 798
AfiI CCNNNNNNNGG 5 cut(s) 140, 272, 554, 621, 973
AgsI TTSAA 2 cut(s) 233, 662
AjiI CACGTC 1 cut(s) 113
AjnI CCWGG 3 cut(s) 133, 265, 521
AjuI GAANNNNNNNTTGG 2 cut(s) 132, 164
AleI CACNNNNGTG 3 cut(s) 322, 437, 671
AluBI AGCT 3 cut(s) 322, 574, 654
AluI AGCT 3 cut(s) 322, 574, 654
Alw26I GTCTC 1 cut(s) 473
AlwI GGATC 3 cut(s) 395, 519, 539
Ama87I CYCGRG 1 cut(s) 553
Aor13HI TCCGGA 1 cut(s) 999
AoxI GGCC 1 cut(s) 136
ApeKI GCWGC 2 cut(s) 293, 571
ApoI RAATTY 1 cut(s) 645
AspLEI GCGC 4 cut(s) 280, 364, 395, 831
AspS9I GGNCC 1 cut(s) 263
AsuC2I CCSGG 2 cut(s) 554, 555
AsuHPI GGTGA 1 cut(s) 451
AvaI CYCGRG 1 cut(s) 553
AvaII GGWCC 1 cut(s) 263
BalI TGGCCA 1 cut(s) 138
BbsI GAAGAC 1 cut(s) 929
BbvI GCAGC 2 cut(s) 280, 583
BccI CCATC 4 cut(s) 83, 180, 367, 619
BceAI ACGGC 2 cut(s) 175, 599
BciT130I CCWGG 3 cut(s) 135, 267, 523
BcnI CCSGG 2 cut(s) 554, 555
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 2 cut(s) 448, 705
BfoI RGCGCY 2 cut(s) 281, 832
BisI GCNGC 3 cut(s) 281, 294, 572
BlsI GCNGC 3 cut(s) 282, 295, 573
Bme1390I CCNGG 5 cut(s) 135, 267, 523, 554, 555
Bme18I GGWCC 1 cut(s) 263
BmeT110I CYCGRG 1 cut(s) 553
BmgBI CACGTC 1 cut(s) 113
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 1 cut(s) 998
BmrFI CCNGG 5 cut(s) 135, 267, 523, 554, 555
BpiI GAAGAC 1 cut(s) 929
BpmI CTGGAG 1 cut(s) 1045
BpuMI CCSGG 2 cut(s) 554, 555
Bsa29I ATCGAT 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 4 cut(s) 139, 522, 552, 553
BsaWI WCCGGW 1 cut(s) 999
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
Bsc4I CCNNNNNNNGG 5 cut(s) 140, 272, 554, 621, 973
Bse1I ACTGG 2 cut(s) 394, 937
Bse3DI GCAATG 4 cut(s) 750, 777, 829, 924
BseAI TCCGGA 1 cut(s) 999
BseBI CCWGG 3 cut(s) 135, 267, 523
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 4 cut(s) 139, 522, 552, 553
BseGI GGATG 2 cut(s) 630, 634
BseLI CCNNNNNNNGG 5 cut(s) 140, 272, 554, 621, 973
BseMI GCAATG 4 cut(s) 750, 777, 829, 924
BseMII CTCAG 1 cut(s) 641
BseNI ACTGG 2 cut(s) 394, 937
BseRI GAGGAG 1 cut(s) 1052
BseXI GCAGC 2 cut(s) 280, 583
BsgI GTGCAG 2 cut(s) 147, 201
Bsh1236I CGCG 1 cut(s) 395
BshFI GGCC 1 cut(s) 138
BshVI ATCGAT 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 553
BsiSI CCGG 2 cut(s) 554, 1000
BslI CCNNNNNNNGG 5 cut(s) 140, 272, 554, 621, 973
BsmAI GTCTC 1 cut(s) 473
BsmI GAATGC 1 cut(s) 701
BsnI GGCC 1 cut(s) 138
Bso31I GGTCTC 1 cut(s) 473
BsoBI CYCGRG 1 cut(s) 553
Bsp13I TCCGGA 1 cut(s) 999
Bsp1407I TGTACA 1 cut(s) 11
Bsp143I GATC 4 cut(s) 237, 400, 511, 544
BspACI CCGC 2 cut(s) 281, 395
BspANI GGCC 1 cut(s) 138
BspCNI CTCAG 1 cut(s) 642
BspDI ATCGAT 1 cut(s) 240
BspEI TCCGGA 1 cut(s) 999
BspFNI CGCG 1 cut(s) 395
BspLI GGNNCC 1 cut(s) 998
BspMAI CTGCAG 1 cut(s) 709
BspPI GGATC 3 cut(s) 395, 519, 539
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 4 cut(s) 750, 777, 829, 924
BsrGI TGTACA 1 cut(s) 11
BsrI ACTGG 2 cut(s) 394, 937
BssECI CCNNGG 4 cut(s) 139, 522, 552, 553
BssMI GATC 4 cut(s) 237, 400, 511, 544
BssT1I CCWWGG 1 cut(s) 139
Bst2UI CCWGG 3 cut(s) 135, 267, 523
Bst4CI ACNGT 2 cut(s) 594, 996
BstAPI GCANNNNNTGC 1 cut(s) 923
BstAUI TGTACA 1 cut(s) 11
BstC8I GCNNGC 2 cut(s) 576, 833
BstDEI CTNAG 2 cut(s) 85, 650
BstENI CCTNNNNNAGG 1 cut(s) 138
BstF5I GGATG 2 cut(s) 630, 634
BstFNI CGCG 1 cut(s) 395
BstH2I RGCGCY 2 cut(s) 281, 832
BstHHI GCGC 4 cut(s) 280, 364, 395, 831
BstKTI GATC 4 cut(s) 240, 403, 514, 547
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 4 cut(s) 237, 400, 511, 544
BstMWI GCNNNNNNNGC 2 cut(s) 828, 923
BstNI CCWGG 3 cut(s) 135, 267, 523
BstSCI CCNGG 5 cut(s) 133, 265, 521, 552, 553
BstSFI CTRYAG 2 cut(s) 448, 705
BstUI CGCG 1 cut(s) 395
BstV1I GCAGC 2 cut(s) 280, 583
BstV2I GAAGAC 1 cut(s) 929
BstXI CCANNNNNNTGG 1 cut(s) 673
Bsu15I ATCGAT 1 cut(s) 240
BsuRI GGCC 1 cut(s) 138
BsuTUI ATCGAT 1 cut(s) 240
BtrI CACGTC 1 cut(s) 113
BtsCI GGATG 2 cut(s) 630, 634
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 2 cut(s) 576, 833
CfoI GCGC 4 cut(s) 280, 364, 395, 831
Cfr13I GGNCC 1 cut(s) 263
Cfr9I CCCGGG 1 cut(s) 553
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 2 cut(s) 12, 797
CviAII CATG 2 cut(s) 853, 953
CviJI RGCY 7 cut(s) 138, 322, 334, 551, 574, 654, 665
CviKI_1 RGCY 7 cut(s) 138, 322, 334, 551, 574, 654, 665
CviQI GTAC 2 cut(s) 12, 797
DdeI CTNAG 2 cut(s) 85, 650
DpnI GATC 4 cut(s) 239, 402, 513, 546
DpnII GATC 4 cut(s) 237, 400, 511, 544
DraIII CACNNNGTG 1 cut(s) 782
EaeI YGGCCR 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 139
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 710
Eco88I CYCGRG 1 cut(s) 553
EcoNI CCTNNNNNAGG 1 cut(s) 138
EcoO109I RGGNCCY 1 cut(s) 263
EcoRI GAATTC 1 cut(s) 645
EcoRII CCWGG 3 cut(s) 133, 265, 521
EcoT14I CCWWGG 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 2 cut(s) 856, 956
FalI AAGNNNNNCTT 4 cut(s) 132, 164, 213, 245
FatI CATG 2 cut(s) 852, 952
Fnu4HI GCNGC 3 cut(s) 281, 294, 572
FokI GGATG 2 cut(s) 637, 641
Fsp4HI GCNGC 3 cut(s) 281, 294, 572
FspBI CTAG 1 cut(s) 38
FspI TGCGCA 1 cut(s) 363
GlaI GCGC 4 cut(s) 279, 363, 394, 830
GluI GCNGC 3 cut(s) 281, 294, 572
GsuI CTGGAG 1 cut(s) 1045
HaeII RGCGCY 2 cut(s) 281, 832
HaeIII GGCC 1 cut(s) 138
HapII CCGG 2 cut(s) 554, 1000
HhaI GCGC 4 cut(s) 280, 364, 395, 831
Hin1II CATG 2 cut(s) 856, 956
Hin6I GCGC 4 cut(s) 278, 362, 393, 829
HinP1I GCGC 4 cut(s) 278, 362, 393, 829
HinfI GANTC 3 cut(s) 468, 977, 1040
HpaII CCGG 2 cut(s) 554, 1000
HphI GGTGA 1 cut(s) 451
Hpy166II GTNNAC 1 cut(s) 872
Hpy188I TCNGA 1 cut(s) 651
Hpy188III TCNNGA 5 cut(s) 233, 370, 728, 1000, 1024
Hpy8I GTNNAC 1 cut(s) 872
HpyAV CCTTC 5 cut(s) 158, 239, 372, 449, 614
HpyCH4III ACNGT 2 cut(s) 594, 996
HpyCH4IV ACGT 2 cut(s) 112, 766
HpyF10VI GCNNNNNNNGC 2 cut(s) 828, 923
HpyF3I CTNAG 2 cut(s) 85, 650
HpySE526I ACGT 2 cut(s) 112, 766
Hsp92II CATG 2 cut(s) 856, 956
HspAI GCGC 4 cut(s) 278, 362, 393, 829
Kpn2I TCCGGA 1 cut(s) 999
Kzo9I GATC 4 cut(s) 237, 400, 511, 544
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 2 cut(s) 280, 583
MaeI CTAG 1 cut(s) 38
MaeII ACGT 2 cut(s) 112, 766
MaeIII GTNAC 6 cut(s) 79, 113, 223, 251, 385, 782
MalI GATC 4 cut(s) 239, 402, 513, 546
MboI GATC 4 cut(s) 237, 400, 511, 544
MboII GAAGA 3 cut(s) 34, 340, 929
MlsI TGGCCA 1 cut(s) 138
MluCI AATT 3 cut(s) 4, 502, 645
MluNI TGGCCA 1 cut(s) 138
MlyI GAGTC 1 cut(s) 1049
MmeI TCCRAC 2 cut(s) 202, 478
MnlI CCTC 6 cut(s) 40, 141, 453, 860, 967, 1030
Mox20I TGGCCA 1 cut(s) 138
MroI TCCGGA 1 cut(s) 999
MscI TGGCCA 1 cut(s) 138
MseI TTAA 2 cut(s) 198, 348
MslI CAYNNNNRTG 3 cut(s) 322, 437, 671
Msp20I TGGCCA 1 cut(s) 138
MspA1I CMGCKG 1 cut(s) 322
MspI CCGG 2 cut(s) 554, 1000
MspR9I CCNGG 5 cut(s) 135, 267, 523, 554, 555
Mva1269I GAATGC 1 cut(s) 701
MvaI CCWGG 3 cut(s) 135, 267, 523
MvnI CGCG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 828, 923
NciI CCSGG 2 cut(s) 554, 555
NdeII GATC 4 cut(s) 237, 400, 511, 544
NlaIII CATG 2 cut(s) 856, 956
NlaIV GGNNCC 1 cut(s) 998
NmuCI GTSAC 4 cut(s) 79, 113, 385, 782
NsbI TGCGCA 1 cut(s) 363
OliI CACNNNNGTG 3 cut(s) 322, 437, 671
PcsI WCGNNNNNNNCGW 1 cut(s) 871
PctI GAATGC 1 cut(s) 701
PfeI GAWTC 2 cut(s) 468, 977
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 3 cut(s) 282, 295, 573
PleI GAGTC 1 cut(s) 1048
PpsI GAGTC 1 cut(s) 1048
PpuMI RGGWCCY 1 cut(s) 263
Psp5II RGGWCCY 1 cut(s) 263
Psp6I CCWGG 3 cut(s) 133, 265, 521
PspGI CCWGG 3 cut(s) 133, 265, 521
PspN4I GGNNCC 1 cut(s) 998
PspPI GGNCC 1 cut(s) 263
PspPPI RGGWCCY 1 cut(s) 263
PstI CTGCAG 1 cut(s) 709
PvuII CAGCTG 1 cut(s) 322
RsaI GTAC 2 cut(s) 13, 798
RsaNI GTAC 2 cut(s) 12, 797
RseI CAYNNNNRTG 3 cut(s) 322, 437, 671
SaqAI TTAA 2 cut(s) 198, 348
SatI GCNGC 3 cut(s) 281, 294, 572
Sau3AI GATC 4 cut(s) 237, 400, 511, 544
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 1 cut(s) 1049
ScrFI CCNGG 5 cut(s) 135, 267, 523, 554, 555
SfcI CTRYAG 2 cut(s) 448, 705
SinI GGWCC 1 cut(s) 263
SmaI CCCGGG 1 cut(s) 555
SmiMI CAYNNNNRTG 3 cut(s) 322, 437, 671
Sse9I AATT 3 cut(s) 4, 502, 645
SsiI CCGC 2 cut(s) 281, 395
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 5 cut(s) 133, 265, 521, 552, 553
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 2 cut(s) 594, 996
TaiI ACGT 2 cut(s) 115, 769
TaqI TCGA 4 cut(s) 240, 369, 466, 980
TasI AATT 3 cut(s) 4, 502, 645
TatI WGTACW 2 cut(s) 11, 796
TauI GCSGC 1 cut(s) 283
TfiI GAWTC 2 cut(s) 468, 977
Tru1I TTAA 2 cut(s) 198, 348
Tru9I TTAA 2 cut(s) 198, 348
TscAI CASTG 1 cut(s) 673
TseFI GTSAC 4 cut(s) 79, 113, 385, 782
TseI GCWGC 2 cut(s) 293, 571
Tsp45I GTSAC 4 cut(s) 79, 113, 385, 782
TspDTI ATGAA 5 cut(s) 17, 35, 708, 860, 1023
TspMI CCCGGG 1 cut(s) 553
TspRI CASTG 1 cut(s) 673
VpaK11BI GGWCC 1 cut(s) 263
XagI CCTNNNNNAGG 1 cut(s) 138
XapI RAATTY 1 cut(s) 645
XmaI CCCGGG 1 cut(s) 553
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.