pycom17g22410

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0014229
Physical Location & Seq
Forward (+)
20806816 .. 20808964
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22410.2

Sequence Viewer

Length: 1062 bp
ATGAAGAAAATGATAGCTCTAGGGTTCGAGGGTTCAGCCAACAAGATTGGTGTCGGGGTCGTCACTTTAGATGGTACAATTTTGTCGAACCCACGCCACACATACATTACTCCTCCCGGTCAAGGGTTCCTTCCCCGGGAAACTGCACAACATCACTTCCAACACATCCTCCCGCTGGTCAAATCTGCTTTGGAAACTGCCCAAATAACGCCTAAGGAAATTGATTGCCTCTGTTACACCAAGGGTCCTGGCATGGGAGCACCACTACAAGTTGCTGCCATTGTTGTGAGGGTTCTTTCACAGCTGTGGAAGAAGCCCATTGTTGCAGTTAATCACTGTATTGCACATATTGAGATGGGAAGGGTTGTGACCGGTGCAGATGATCCTTTTGTGTTGTATGTTAGTGGTGGGAACACACAGGTAATTGCATATAGTGAAGGGAGGTATCGAATCTTTGGCGAGACCATTGATATCGCTGTTGGAAACTGCTTGGATCGGTTCGCCAGGGTATTAACACTCTCCAATGATCCAGCCCCGGGATATAACATTGAGCAGCTTGCAAAGAAAGGAGAACAGTTTATAGATCTTCCTTACGTTGTCAAAGGAATGGATGTGTCTTTTAGTGGAATTTTGAGCTATATTGAAGCTACTGCTGTGGAAAAGCTCAAGAATAACGAGTGCACACCTGCAGACCTGTGCTACTCGCTTCAGGAAAATGTGTTTGCAATGCTTGTGGAGATAACAGAACGTGCAATGGCGCATTGTGACAAGAAAGATGTTCTTATTGTTGGTGGTGTGGGTTGCAATGAACGGTTGCAAGAGATGATGAGGACCATGTGCACCGAACGGGGTGGAAGGTTGTTTGCAACTGATGATAGGTATTGTATTGACAATGGGGCCATGATTGCTTATACTGGTCTTCTTGCTTTTGCCAATGGCACATCAACCCCGCTGGAGGAATCAACTTTTACCCAACGGTTTCGTACTGATGACGTTGAAGCCGTCTGGAGAATAAAAGAAGAGTCAGAAAAAGTAAATGGGTTGAAGAATGAGAGTACCTGA

Protein Analysis

354

Amino Acids

38.79

Weight (kDa)

5.6

Isoelectric Point (pI)

30.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22720 AT4G22720
fragaria_vesca FvH4_3g16550 FvH4_3g16550
malus_domestica MD17G1219400.v1.1
prunus_persica Prupe.1G196300_v2.0.a1
pyrus_communis pycom17g22410
rosa_chinensis RchiOBHm_Chr1g0331181
rosa_laevigata RLG00000029747
rosa_multiflora Rmu_sc0005711.1_g000004
rosa_roxburghii Rroxscaffold_4G00319280
rosa_rugosa Rorug01G0092100
rosa_samantha Rh1AG116100 Rh1BG087800 Rh1CG111600 Rh1DG120500
rosa_wichuraiana Rw1G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 694
Acc36I ACCTGC 1 cut(s) 694
AciI CCGC 2 cut(s) 173, 950
AclWI GGATC 3 cut(s) 377, 501, 521
AcsI RAATTY 1 cut(s) 627
AcuI CTGAAG 1 cut(s) 692
AfaI GTAC 3 cut(s) 76, 985, 1057
AfiI CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 955
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 3 cut(s) 644, 998, 1045
AjnI CCWGG 2 cut(s) 247, 503
AleI CACNNNNGTG 1 cut(s) 304
AluBI AGCT 6 cut(s) 17, 304, 556, 636, 647, 664
AluI AGCT 6 cut(s) 17, 304, 556, 636, 647, 664
Alw21I GWGCWC 3 cut(s) 262, 683, 842
Alw26I GTCTC 1 cut(s) 455
Alw44I GTGCAC 2 cut(s) 679, 838
AlwI GGATC 3 cut(s) 377, 501, 521
Ama87I CYCGRG 2 cut(s) 135, 535
AoxI GGCC 1 cut(s) 897
ApaLI GTGCAC 2 cut(s) 679, 838
ApeKI GCWGC 2 cut(s) 275, 553
ApoI RAATTY 1 cut(s) 627
AsiGI ACCGGT 1 cut(s) 371
AspLEI GCGC 1 cut(s) 760
AspS9I GGNCC 3 cut(s) 245, 831, 897
AsuC2I CCSGG 5 cut(s) 117, 136, 137, 536, 537
AvaI CYCGRG 2 cut(s) 135, 535
AvaII GGWCC 2 cut(s) 245, 831
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 2 cut(s) 683, 842
BbsI GAAGAC 1 cut(s) 911
Bbv12I GWGCWC 3 cut(s) 262, 683, 842
BbvI GCAGC 2 cut(s) 262, 565
BccI CCATC 2 cut(s) 65, 349
BceAI ACGGC 1 cut(s) 986
BciT130I CCWGG 2 cut(s) 249, 505
BcnI CCSGG 5 cut(s) 117, 136, 137, 536, 537
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 694
BglII AGATCT 1 cut(s) 583
BisI GCNGC 2 cut(s) 276, 554
BlsI GCNGC 2 cut(s) 277, 555
Bme1390I CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
Bme18I GGWCC 2 cut(s) 245, 831
BmeT110I CYCGRG 2 cut(s) 135, 535
BmgT120I GGNCC 3 cut(s) 245, 831, 897
BmiI GGNNCC 3 cut(s) 128, 246, 898
BmrFI CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
BpiI GAAGAC 1 cut(s) 911
BpmI CTGGAG 2 cut(s) 974, 1027
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 5 cut(s) 117, 136, 137, 536, 537
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 6 cut(s) 134, 135, 240, 504, 534, 535
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 955
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 919
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 3 cut(s) 732, 759, 811
BseBI CCWGG 2 cut(s) 249, 505
BseDI CCNNGG 6 cut(s) 134, 135, 240, 504, 534, 535
BseGI GGATG 2 cut(s) 165, 616
BseLI CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 955
BseMI GCAATG 3 cut(s) 732, 759, 811
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 1 cut(s) 102
BseSI GKGCMC 2 cut(s) 683, 842
BseXI GCAGC 2 cut(s) 262, 565
BsgI GTGCAG 2 cut(s) 129, 396
BshFI GGCC 1 cut(s) 899
BshTI ACCGGT 1 cut(s) 371
BsiHKAI GWGCWC 3 cut(s) 262, 683, 842
BsiHKCI CYCGRG 2 cut(s) 135, 535
BsiSI CCGG 4 cut(s) 117, 136, 372, 536
BslI CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 955
BsmAI GTCTC 1 cut(s) 455
BsnI GGCC 1 cut(s) 899
Bso31I GGTCTC 1 cut(s) 455
BsoBI CYCGRG 2 cut(s) 135, 535
Bsp1286I GDGCHC 3 cut(s) 262, 683, 842
Bsp143I GATC 4 cut(s) 382, 493, 526, 583
BspACI CCGC 2 cut(s) 173, 950
BspANI GGCC 1 cut(s) 899
BspLI GGNNCC 3 cut(s) 128, 246, 898
BspMAI CTGCAG 1 cut(s) 691
BspMI ACCTGC 1 cut(s) 694
BspPI GGATC 3 cut(s) 377, 501, 521
BspTNI GGTCTC 1 cut(s) 455
BsrDI GCAATG 3 cut(s) 732, 759, 811
BsrFI RCCGGY 1 cut(s) 371
BsrI ACTGG 1 cut(s) 919
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 6 cut(s) 134, 135, 240, 504, 534, 535
BssMI GATC 4 cut(s) 382, 493, 526, 583
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 2 cut(s) 249, 505
Bst4CI ACNGT 4 cut(s) 338, 576, 813, 978
Bst6I CTCTTC 1 cut(s) 1014
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 1 cut(s) 213
BstF5I GGATG 2 cut(s) 165, 616
BstHHI GCGC 1 cut(s) 760
BstKTI GATC 4 cut(s) 385, 496, 529, 586
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 4 cut(s) 382, 493, 526, 583
BstMWI GCNNNNNNNGC 1 cut(s) 905
BstNI CCWGG 2 cut(s) 249, 505
BstSCI CCNGG 7 cut(s) 115, 134, 135, 247, 503, 534, 535
BstSFI CTRYAG 1 cut(s) 687
BstSLI GKGCMC 2 cut(s) 683, 842
BstV1I GCAGC 2 cut(s) 262, 565
BstV2I GAAGAC 1 cut(s) 911
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
Bsu36I CCTNAGG 1 cut(s) 213
BsuRI GGCC 1 cut(s) 899
BtsCI GGATG 2 cut(s) 165, 616
BtsIMutI CAGTG 1 cut(s) 334
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 1 cut(s) 558
CfoI GCGC 1 cut(s) 760
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 245, 831, 897
Cfr9I CCCGGG 2 cut(s) 135, 535
Csp6I GTAC 3 cut(s) 75, 984, 1056
CspAI ACCGGT 1 cut(s) 371
CspCI CAANNNNNGTGG 2 cut(s) 714, 749
CviAII CATG 3 cut(s) 253, 835, 901
CviQI GTAC 3 cut(s) 75, 984, 1056
DdeI CTNAG 1 cut(s) 213
DpnI GATC 4 cut(s) 384, 495, 528, 585
DpnII GATC 4 cut(s) 382, 493, 526, 583
Eam1104I CTCTTC 1 cut(s) 1014
EarI CTCTTC 1 cut(s) 1014
Eco130I CCWWGG 1 cut(s) 240
Eco31I GGTCTC 1 cut(s) 455
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 2 cut(s) 245, 831
Eco57I CTGAAG 1 cut(s) 692
Eco81I CCTNAGG 1 cut(s) 213
Eco88I CYCGRG 2 cut(s) 135, 535
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 2 cut(s) 247, 503
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 3 cut(s) 256, 838, 904
FalI AAGNNNNNCTT 4 cut(s) 114, 146, 765, 797
FatI CATG 3 cut(s) 252, 834, 900
FauI CCCGC 2 cut(s) 180, 957
Fnu4HI GCNGC 2 cut(s) 276, 554
FokI GGATG 2 cut(s) 152, 623
Fsp4HI GCNGC 2 cut(s) 276, 554
FspBI CTAG 1 cut(s) 20
GlaI GCGC 1 cut(s) 759
GluI GCNGC 2 cut(s) 276, 554
GsuI CTGGAG 2 cut(s) 974, 1027
HaeIII GGCC 1 cut(s) 899
HapII CCGG 4 cut(s) 117, 136, 372, 536
HhaI GCGC 1 cut(s) 760
Hin1II CATG 3 cut(s) 256, 838, 904
Hin6I GCGC 1 cut(s) 758
HinP1I GCGC 1 cut(s) 758
HinfI GANTC 3 cut(s) 450, 959, 1022
HpaII CCGG 4 cut(s) 117, 136, 372, 536
Hpy166II GTNNAC 2 cut(s) 681, 840
Hpy188I TCNGA 1 cut(s) 1027
Hpy188III TCNNGA 3 cut(s) 667, 710, 1006
Hpy8I GTNNAC 2 cut(s) 681, 840
HpyAV CCTTC 4 cut(s) 140, 354, 431, 849
HpyCH4III ACNGT 4 cut(s) 338, 576, 813, 978
HpyCH4IV ACGT 3 cut(s) 594, 748, 993
HpyF10VI GCNNNNNNNGC 1 cut(s) 905
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 3 cut(s) 594, 748, 993
Hsp92II CATG 3 cut(s) 256, 838, 904
HspAI GCGC 1 cut(s) 758
Kzo9I GATC 4 cut(s) 382, 493, 526, 583
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 2 cut(s) 262, 565
MaeI CTAG 1 cut(s) 20
MaeII ACGT 3 cut(s) 594, 748, 993
MaeIII GTNAC 4 cut(s) 61, 233, 367, 764
MalI GATC 4 cut(s) 384, 495, 528, 585
MboI GATC 4 cut(s) 382, 493, 526, 583
MboII GAAGA 6 cut(s) 16, 322, 578, 911, 1031, 1057
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 3 cut(s) 262, 683, 842
MluCI AATT 4 cut(s) 78, 219, 423, 627
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 2 cut(s) 184, 460
MnlI CCTC 8 cut(s) 22, 123, 179, 239, 282, 435, 822, 949
MseI TTAA 2 cut(s) 330, 512
MslI CAYNNNNRTG 2 cut(s) 284, 304
MspA1I CMGCKG 3 cut(s) 175, 304, 952
MspI CCGG 4 cut(s) 117, 136, 372, 536
MspR9I CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
MvaI CCWGG 2 cut(s) 249, 505
MwoI GCNNNNNNNGC 1 cut(s) 905
NciI CCSGG 5 cut(s) 117, 136, 137, 536, 537
NdeII GATC 4 cut(s) 382, 493, 526, 583
NlaIII CATG 3 cut(s) 256, 838, 904
NlaIV GGNNCC 3 cut(s) 128, 246, 898
NmuCI GTSAC 3 cut(s) 61, 367, 764
OliI CACNNNNGTG 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 694
PcsI WCGNNNNNNNCGW 1 cut(s) 999
PfeI GAWTC 2 cut(s) 450, 959
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 2 cut(s) 277, 555
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
PpuMI RGGWCCY 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 247, 503
PspGI CCWGG 2 cut(s) 247, 503
PspN4I GGNNCC 3 cut(s) 128, 246, 898
PspPI GGNCC 3 cut(s) 245, 831, 897
PspPPI RGGWCCY 1 cut(s) 245
PstI CTGCAG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 3 cut(s) 76, 985, 1057
RsaNI GTAC 3 cut(s) 75, 984, 1056
RseI CAYNNNNRTG 2 cut(s) 284, 304
SaqAI TTAA 2 cut(s) 330, 512
SatI GCNGC 2 cut(s) 276, 554
Sau3AI GATC 4 cut(s) 382, 493, 526, 583
Sau96I GGNCC 3 cut(s) 245, 831, 897
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
SduI GDGCHC 3 cut(s) 262, 683, 842
SfcI CTRYAG 1 cut(s) 687
SinI GGWCC 2 cut(s) 245, 831
SmaI CCCGGG 2 cut(s) 137, 537
SmiMI CAYNNNNRTG 2 cut(s) 284, 304
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 4 cut(s) 78, 219, 423, 627
SsiI CCGC 2 cut(s) 173, 950
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 7 cut(s) 115, 134, 135, 247, 503, 534, 535
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 4 cut(s) 338, 576, 813, 978
TaiI ACGT 3 cut(s) 597, 751, 996
TaqI TCGA 3 cut(s) 27, 86, 448
TasI AATT 4 cut(s) 78, 219, 423, 627
TfiI GAWTC 2 cut(s) 450, 959
Tru1I TTAA 2 cut(s) 330, 512
Tru9I TTAA 2 cut(s) 330, 512
TscAI CASTG 1 cut(s) 341
TseFI GTSAC 3 cut(s) 61, 367, 764
TseI GCWGC 2 cut(s) 275, 553
Tsp45I GTSAC 3 cut(s) 61, 367, 764
TspDTI ATGAA 2 cut(s) 17, 822
TspMI CCCGGG 2 cut(s) 135, 535
TspRI CASTG 1 cut(s) 341
VneI GTGCAC 2 cut(s) 679, 838
VpaK11BI GGWCC 2 cut(s) 245, 831
XapI RAATTY 1 cut(s) 627
XmaI CCCGGG 2 cut(s) 135, 535
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.