AT4G22720

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0014229
Physical Location & Seq
Forward (+)
11936942 .. 11938969
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G22720.2

Sequence Viewer

Length: 1062 bp
ATGAAGAAGAAGATGATAGCCATTGGTTTCGAAGGATCAGCTAACAAGATTGGAGTTGGAATTGTGACTTTAGACGGTACTATCTTAGCAAATCCTCGGCATACGTATATAACACCACCTGGTCATGGTTTTCTTCCTAGAGAGACTGCACATCACCATCTTGACCATGTTCTTCCTTTAGTCAAATCAGCTTTAGAGACTTCTCAAGTTACTCCTGAAGAAATCGACTGTATTTGTTATACGAAAGGTCCCGGGATGGGAGCGCCGTTGCAGGTTTCAGCCATTGTTGTTAGAGTATTGTCACAGCTTTGGAAAAAGCCAATTGTTGCTGTGAATCATTGTGTGGCTCACATTGAGATGGGGAGAGTAGTTACTGGAGCTGATGATCCTGTTGTGTTGTATGTGAGTGGTGGTAACACTCAGGTGATTGCTTATAGTGAAGGTAGGTACCGGATTTTTGGTGAGACTATTGATATTGCTGTTGGTAATTGTTTGGATCGGTTTGCTAGAGTCTTGAAGCTTTCTAATGATCCAAGCCCTGGTTATAACATTGAACAGCTTGCTAAGAAAGGAGAAAACTTTATTGATCTTCCGTATGCTGTTAAAGGTATGGATGTATCGTTCAGCGGAATCTTGAGTTATATCGAAACTACTGCAGAGGAGAAGCTCAAGAATAACGAGTGTACACCAGCAGACTTGTGTTATTCCCTTCAAGAGACGGTGTTTGCGATGTTGGTTGAGATCACAGAAAGAGCGATGGCGCATTGTGACAAGAAAGACGTTTTGATAGTTGGCGGTGTTGGGTGTAACGAGCGTTTGCAGGAAATGATGAGGACTATGTGCTCAGAACGTGATGGTAAGCTGTTTGCAACAGATGACCGTTACTGCATCGATAATGGAGCTATGATCGCTTACACAGGTCTACTCGCGTTTGTTAATGGTATTGAAACACCGATCGAAGACTCTACCTTTACACAACGATTTCGCACAGACGAGGTTCATGCGGTGTGGCGAGAGAAAGAAGCTGTACTACTTGGGGATAAGAAAGTTGCAGCGAATTGA

Protein Analysis

353

Amino Acids

38.77

Weight (kDa)

5.62

Isoelectric Point (pI)

28.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TsaD PF00814 33 - 305 4.8e-85 tRNA N6-adenosine threonylcarbamoyltransferase
HypF_C_2 PF22521 178 - 298 1.2e-06 Carbamoyltransferase, Kae1-like Domain, second subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22720 AT4G22720
fragaria_vesca FvH4_3g16550 FvH4_3g16550
malus_domestica MD17G1219400.v1.1
prunus_persica Prupe.1G196300_v2.0.a1
pyrus_communis pycom17g22410
rosa_chinensis RchiOBHm_Chr1g0331181
rosa_laevigata RLG00000029747
rosa_multiflora Rmu_sc0005711.1_g000004
rosa_roxburghii Rroxscaffold_4G00319280
rosa_rugosa Rorug01G0092100
rosa_samantha Rh1AG116100 Rh1BG087800 Rh1CG111600 Rh1DG120500
rosa_wichuraiana Rw1G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 546
Acc36I ACCTGC 1 cut(s) 262
Acc65I GGTACC 1 cut(s) 447
AccB1I GGYRCC 1 cut(s) 447
AccB7I CCANNNNNTGG 1 cut(s) 539
AccI GTMKAC 1 cut(s) 922
AccII CGCG 1 cut(s) 929
AciI CCGC 3 cut(s) 627, 795, 1004
AclWI GGATC 4 cut(s) 43, 380, 504, 524
AcuI CTGAAG 1 cut(s) 237
AfaI GTAC 4 cut(s) 79, 449, 685, 1029
AfiI CCNNNNNNNGG 3 cut(s) 125, 257, 539
AgsI TTSAA 4 cut(s) 517, 554, 713, 947
AjnI CCWGG 2 cut(s) 118, 538
AleI CACNNNNGTG 1 cut(s) 422
AloI GAACNNNNNNTCC 2 cut(s) 605, 637
Alw21I GWGCWC 1 cut(s) 845
Alw26I GTCTC 4 cut(s) 137, 191, 458, 710
AlwI GGATC 4 cut(s) 43, 380, 504, 524
Ama87I CYCGRG 1 cut(s) 251
ApeKI GCWGC 1 cut(s) 1052
Asp718I GGTACC 1 cut(s) 447
AspLEI GCGC 2 cut(s) 265, 763
AspS9I GGNCC 1 cut(s) 248
AsuC2I CCSGG 2 cut(s) 252, 253
AsuHPI GGTGA 3 cut(s) 146, 436, 473
AsuII TTCGAA 1 cut(s) 30
AvaI CYCGRG 1 cut(s) 251
AvaII GGWCC 1 cut(s) 248
BanI GGYRCC 1 cut(s) 447
BarI GAAGNNNNNNTAC 2 cut(s) 1014, 1046
BbsI GAAGAC 1 cut(s) 966
Bbv12I GWGCWC 1 cut(s) 845
BccI CCATC 5 cut(s) 165, 250, 352, 751, 848
BceAI ACGGC 1 cut(s) 250
BcgI CGANNNNNNTGC 4 cut(s) 635, 669, 983, 1017
BciT130I CCWGG 2 cut(s) 120, 540
BcnI CCSGG 2 cut(s) 252, 253
BcoDI GTCTC 4 cut(s) 137, 191, 458, 710
BfaI CTAG 2 cut(s) 138, 507
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 266
BfuAI ACCTGC 1 cut(s) 262
BisI GCNGC 1 cut(s) 1053
BlsI GCNGC 1 cut(s) 1054
Bme1390I CCNGG 4 cut(s) 120, 252, 253, 540
Bme18I GGWCC 1 cut(s) 248
BmeT110I CYCGRG 1 cut(s) 251
BmgT120I GGNCC 1 cut(s) 248
BmiI GGNNCC 2 cut(s) 250, 449
BmrFI CCNGG 4 cut(s) 120, 252, 253, 540
BmsI GCATC 1 cut(s) 897
BpiI GAAGAC 1 cut(s) 966
BpmI CTGGAG 1 cut(s) 396
Bpu14I TTCGAA 1 cut(s) 30
BpuEI CTTGAG 3 cut(s) 189, 653, 655
BpuMI CCSGG 2 cut(s) 252, 253
Bsa29I ATCGAT 1 cut(s) 891
BsaAI YACGTR 1 cut(s) 105
BsaJI CCNNGG 3 cut(s) 95, 251, 538
BsaWI WCCGGW 1 cut(s) 450
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 257, 539
Bse1I ACTGG 1 cut(s) 379
BseBI CCWGG 2 cut(s) 120, 540
BseCI ATCGAT 1 cut(s) 891
BseDI CCNNGG 3 cut(s) 95, 251, 538
BseGI GGATG 2 cut(s) 261, 619
BseLI CCNNNNNNNGG 3 cut(s) 125, 257, 539
BseMII CTCAG 2 cut(s) 434, 858
BseNI ACTGG 1 cut(s) 379
BseRI GAGGAG 1 cut(s) 674
BsgI GTGCAG 1 cut(s) 132
Bsh1236I CGCG 1 cut(s) 929
Bsh1285I CGRYCG 1 cut(s) 957
BshNI GGYRCC 1 cut(s) 447
BshVI ATCGAT 1 cut(s) 891
BsiEI CGRYCG 1 cut(s) 957
BsiHKAI GWGCWC 1 cut(s) 845
BsiHKCI CYCGRG 1 cut(s) 251
BsiSI CCGG 2 cut(s) 252, 451
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 3 cut(s) 125, 257, 539
BsmAI GTCTC 4 cut(s) 137, 191, 458, 710
BsmBI CGTCTC 1 cut(s) 710
BsmFI GGGAC 1 cut(s) 234
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 845
Bsp1407I TGTACA 1 cut(s) 683
Bsp143I GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
BspACI CCGC 3 cut(s) 627, 795, 1004
BspCNI CTCAG 2 cut(s) 433, 857
BspDI ATCGAT 1 cut(s) 891
BspFNI CGCG 1 cut(s) 929
BspLI GGNNCC 2 cut(s) 250, 449
BspMAI CTGCAG 1 cut(s) 658
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 4 cut(s) 43, 380, 504, 524
BspT104I TTCGAA 1 cut(s) 30
BspT107I GGYRCC 1 cut(s) 447
BsrGI TGTACA 1 cut(s) 683
BsrI ACTGG 1 cut(s) 379
BssECI CCNNGG 3 cut(s) 95, 251, 538
BssMI GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
Bst2UI CCWGG 2 cut(s) 120, 540
Bst4CI ACNGT 4 cut(s) 77, 230, 721, 881
BstAUI TGTACA 1 cut(s) 683
BstBAI YACGTR 1 cut(s) 105
BstBI TTCGAA 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 561
BstDEI CTNAG 4 cut(s) 85, 420, 564, 844
BstF5I GGATG 2 cut(s) 261, 619
BstFNI CGCG 1 cut(s) 929
BstH2I RGCGCY 1 cut(s) 266
BstHHI GCGC 2 cut(s) 265, 763
BstKTI GATC 8 cut(s) 38, 388, 499, 532, 589, 744, 909, 957
BstMAI GTCTC 4 cut(s) 137, 191, 458, 710
BstMBI GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
BstMCI CGRYCG 1 cut(s) 957
BstMWI GCNNNNNNNGC 1 cut(s) 908
BstNI CCWGG 2 cut(s) 120, 540
BstSCI CCNGG 4 cut(s) 118, 250, 251, 538
BstSFI CTRYAG 1 cut(s) 654
BstSNI TACGTA 1 cut(s) 105
BstUI CGCG 1 cut(s) 929
BstV2I GAAGAC 1 cut(s) 966
Bsu15I ATCGAT 1 cut(s) 891
BsuTUI ATCGAT 1 cut(s) 891
BtgZI GCGATG 2 cut(s) 743, 770
BtsCI GGATG 2 cut(s) 261, 619
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 1 cut(s) 561
CfoI GCGC 2 cut(s) 265, 763
Cfr13I GGNCC 1 cut(s) 248
Cfr9I CCCGGG 1 cut(s) 251
ClaI ATCGAT 1 cut(s) 891
CsiI ACCWGGT 1 cut(s) 118
Csp6I GTAC 4 cut(s) 78, 448, 684, 1028
CviAII CATG 3 cut(s) 125, 167, 1001
CviQI GTAC 4 cut(s) 78, 448, 684, 1028
DdeI CTNAG 4 cut(s) 85, 420, 564, 844
DpnI GATC 8 cut(s) 37, 387, 498, 531, 588, 743, 908, 956
DpnII GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
Eco105I TACGTA 1 cut(s) 105
Eco47I GGWCC 1 cut(s) 248
Eco57I CTGAAG 1 cut(s) 237
Eco88I CYCGRG 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 118, 538
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 3 cut(s) 128, 170, 1004
FaqI GGGAC 1 cut(s) 234
FatI CATG 3 cut(s) 124, 166, 1000
FblI GTMKAC 1 cut(s) 922
Fnu4HI GCNGC 1 cut(s) 1053
FokI GGATG 2 cut(s) 268, 626
Fsp4HI GCNGC 1 cut(s) 1053
FspBI CTAG 2 cut(s) 138, 507
GlaI GCGC 2 cut(s) 264, 762
GluI GCNGC 1 cut(s) 1053
GsuI CTGGAG 1 cut(s) 396
HaeII RGCGCY 1 cut(s) 266
HapII CCGG 2 cut(s) 252, 451
HhaI GCGC 2 cut(s) 265, 763
Hin1II CATG 3 cut(s) 128, 170, 1004
Hin6I GCGC 2 cut(s) 263, 761
HinP1I GCGC 2 cut(s) 263, 761
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 4 cut(s) 334, 510, 630, 962
HpaII CCGG 2 cut(s) 252, 451
HphI GGTGA 3 cut(s) 146, 436, 473
Hpy166II GTNNAC 3 cut(s) 684, 686, 923
Hpy188I TCNGA 1 cut(s) 847
Hpy188III TCNNGA 6 cut(s) 161, 215, 514, 634, 670, 713
Hpy8I GTNNAC 3 cut(s) 684, 686, 923
HpyAV CCTTC 3 cut(s) 26, 434, 719
HpyCH4III ACNGT 4 cut(s) 77, 230, 721, 881
HpyCH4IV ACGT 3 cut(s) 104, 780, 850
HpyCH4V TGCA 7 cut(s) 149, 271, 656, 820, 869, 888, 1052
HpyF10VI GCNNNNNNNGC 1 cut(s) 908
HpyF3I CTNAG 4 cut(s) 85, 420, 564, 844
HpySE526I ACGT 3 cut(s) 104, 780, 850
Hsp92II CATG 3 cut(s) 128, 170, 1004
HspAI GCGC 2 cut(s) 263, 761
KpnI GGTACC 1 cut(s) 451
Kzo9I GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
LmnI GCTCC 3 cut(s) 260, 377, 899
LweI GCATC 1 cut(s) 897
MabI ACCWGGT 1 cut(s) 118
MaeI CTAG 2 cut(s) 138, 507
MaeII ACGT 3 cut(s) 104, 780, 850
MaeIII GTNAC 8 cut(s) 64, 208, 300, 370, 413, 767, 806, 881
MalI GATC 8 cut(s) 37, 387, 498, 531, 588, 743, 908, 956
MboI GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
MboII GAAGA 8 cut(s) 16, 19, 22, 125, 164, 230, 581, 971
MfeI CAATTG 1 cut(s) 321
MhlI GDGCHC 1 cut(s) 845
MluCI AATT 4 cut(s) 60, 321, 487, 1057
MlyI GAGTC 2 cut(s) 519, 956
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 4 cut(s) 105, 652, 825, 988
MseI TTAA 2 cut(s) 603, 936
MslI CAYNNNNRTG 2 cut(s) 356, 422
MspA1I CMGCKG 1 cut(s) 627
MspI CCGG 2 cut(s) 252, 451
MspR9I CCNGG 4 cut(s) 120, 252, 253, 540
MunI CAATTG 1 cut(s) 321
MvaI CCWGG 2 cut(s) 120, 540
MvnI CGCG 1 cut(s) 929
MwoI GCNNNNNNNGC 1 cut(s) 908
NciI CCSGG 2 cut(s) 252, 253
NdeII GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
NlaIII CATG 3 cut(s) 128, 170, 1004
NlaIV GGNNCC 2 cut(s) 250, 449
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 3 cut(s) 64, 300, 767
NspV TTCGAA 1 cut(s) 30
OliI CACNNNNGTG 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 725
PfeI GAWTC 2 cut(s) 334, 630
PflMI CCANNNNNTGG 1 cut(s) 539
PkrI GCNGC 1 cut(s) 1054
Ple19I CGATCG 1 cut(s) 957
PleI GAGTC 2 cut(s) 518, 956
PpsI GAGTC 2 cut(s) 518, 956
Ppu21I YACGTR 1 cut(s) 105
PpuMI RGGWCCY 1 cut(s) 248
PsiI TTATAA 1 cut(s) 546
Psp5II RGGWCCY 1 cut(s) 248
Psp6I CCWGG 2 cut(s) 118, 538
PspGI CCWGG 2 cut(s) 118, 538
PspN4I GGNNCC 2 cut(s) 250, 449
PspPI GGNCC 1 cut(s) 248
PspPPI RGGWCCY 1 cut(s) 248
PstI CTGCAG 1 cut(s) 658
PvuI CGATCG 1 cut(s) 957
RsaI GTAC 4 cut(s) 79, 449, 685, 1029
RsaNI GTAC 4 cut(s) 78, 448, 684, 1028
RseI CAYNNNNRTG 2 cut(s) 356, 422
SaqAI TTAA 2 cut(s) 603, 936
SatI GCNGC 1 cut(s) 1053
Sau3AI GATC 8 cut(s) 35, 385, 496, 529, 586, 741, 906, 954
Sau96I GGNCC 1 cut(s) 248
SchI GAGTC 2 cut(s) 519, 956
ScrFI CCNGG 4 cut(s) 120, 252, 253, 540
SduI GDGCHC 1 cut(s) 845
SexAI ACCWGGT 1 cut(s) 118
SfaNI GCATC 1 cut(s) 897
SfcI CTRYAG 1 cut(s) 654
SfuI TTCGAA 1 cut(s) 30
SinI GGWCC 1 cut(s) 248
SmaI CCCGGG 1 cut(s) 253
SmiMI CAYNNNNRTG 2 cut(s) 356, 422
SmlI CTYRAG 3 cut(s) 204, 634, 668
SmoI CTYRAG 3 cut(s) 204, 634, 668
SnaBI TACGTA 1 cut(s) 105
Sse9I AATT 4 cut(s) 60, 321, 487, 1057
SsiI CCGC 3 cut(s) 627, 795, 1004
SspMI CTAG 2 cut(s) 138, 507
StyD4I CCNGG 4 cut(s) 118, 250, 251, 538
TaaI ACNGT 4 cut(s) 77, 230, 721, 881
TaiI ACGT 3 cut(s) 107, 783, 853
TaqI TCGA 5 cut(s) 30, 225, 645, 891, 957
TasI AATT 4 cut(s) 60, 321, 487, 1057
TatI WGTACW 2 cut(s) 683, 1027
TfiI GAWTC 2 cut(s) 334, 630
Tru1I TTAA 2 cut(s) 603, 936
Tru9I TTAA 2 cut(s) 603, 936
TseFI GTSAC 3 cut(s) 64, 300, 767
TseI GCWGC 1 cut(s) 1052
Tsp45I GTSAC 3 cut(s) 64, 300, 767
TspDTI ATGAA 2 cut(s) 17, 989
TspGWI ACGGA 1 cut(s) 582
TspMI CCCGGG 1 cut(s) 251
Van91I CCANNNNNTGG 1 cut(s) 539
VpaK11BI GGWCC 1 cut(s) 248
XmaI CCCGGG 1 cut(s) 251
XmiI GTMKAC 1 cut(s) 922
XspI CTAG 2 cut(s) 138, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.