MD17G1219400.v1.1

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0014229
Physical Location & Seq
Forward (+)
26734292 .. 26738106
3815 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1219400.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGAAGAAAATGATAGCTCTAGGGTTTGAGGGTTCAGCCAACAAGATTGGTGTCGGGGTCGTCACTTTAGATGGCACAATTTTGTCGAACCCACGCCACACATACATTACTCCTCCCGGTCAAGGGTTCCTTCCCCGGGAAACTGCACAACATCACTTCCAACACATCCTCCCGCTGGTCAAATCTGCTTTGGAAACTGCCCAAATAACGCCTAAGGAAATTGATTGCCTCTGTTACACCAAGGGTCCCGGCATGGGAGCACCACTACAAGTTGCTGCCATTGTTGTTAGGGTTCTTTCACAGCTGTGGAAGAAGCCCATTGTTGCAGTTAATCACTGTATTGCACATATTGAGATGGGAAGGGTTGTGACCGGTGCAGATGATCCTGTTGTGTTGTATGTTAGTGGTGGGAACACACAGGTAATTGCATATAGTGAAGGGAGGTACCGAATCTTTGGCGAAACCATTGATATCGCTGTTGGAAACTGCTTGGATCGGTTCGCCAGGGTATTAACACTCTCCAATGATCCAGCCCCGGGATATAACATTGAGCAGCTTGCAAAGAAAGGAGAACAGTTTATAGATCTTCCTTATGTTGTCAAAGGAATGGATGTGTCTTTTAGTGGAATTTTGAGCTATATTGAAGCTACTGCTGTGGAAAAGCTCAAGAATAACGAGTGCACACCTGCAGACCTGTGCTACTCGGAAAATGTGTTTGCAATGCTTGTGGAGATAACAGAACGTGCAATGGCACATTGTGACAAGAAAGATGTCCTTATTGTTGGTGGTGTGGGTTGCAATGAACGGTTGCAAGAGATGATGAGGACCATGTGCTCTGAACGGGGTGGAAGGTTGTTTGCAACTGATGATAGGTATTGTATTGACAATGGGGCCATGATTGCTTATACTGGTCTTCTTGCTTTTGCCAATGGCACATCAACCCCGCTGGAGGAATCAACTTTTACCCAACGGTTTCGTACTGATGACGTTGAAGCAGTCTGGAGAATAAAAGAAGAGTCAGAAAAAGTAAATGGGTTGAAGAATGAGAGTACCTGA

Protein Analysis

352

Amino Acids

38.49

Weight (kDa)

5.6

Isoelectric Point (pI)

28.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TsaD PF00814 32 - 302 6e-81 tRNA N6-adenosine threonylcarbamoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22720 AT4G22720
fragaria_vesca FvH4_3g16550 FvH4_3g16550
malus_domestica MD17G1219400.v1.1
prunus_persica Prupe.1G196300_v2.0.a1
pyrus_communis pycom17g22410
rosa_chinensis RchiOBHm_Chr1g0331181
rosa_laevigata RLG00000029747
rosa_multiflora Rmu_sc0005711.1_g000004
rosa_roxburghii Rroxscaffold_4G00319280
rosa_rugosa Rorug01G0092100
rosa_samantha Rh1AG116100 Rh1BG087800 Rh1CG111600 Rh1DG120500
rosa_wichuraiana Rw1G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 694
Acc36I ACCTGC 1 cut(s) 694
Acc65I GGTACC 1 cut(s) 444
AccB1I GGYRCC 1 cut(s) 444
AciI CCGC 2 cut(s) 173, 944
AclWI GGATC 3 cut(s) 377, 501, 521
AcsI RAATTY 1 cut(s) 627
AdeI CACNNNGTG 1 cut(s) 758
AfaI GTAC 3 cut(s) 446, 979, 1051
AfiI CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 949
AgeI ACCGGT 1 cut(s) 371
AgsI TTSAA 3 cut(s) 644, 992, 1039
AjnI CCWGG 1 cut(s) 503
AleI CACNNNNGTG 1 cut(s) 304
AluBI AGCT 6 cut(s) 17, 304, 556, 636, 647, 664
AluI AGCT 6 cut(s) 17, 304, 556, 636, 647, 664
Alw21I GWGCWC 3 cut(s) 262, 683, 836
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 3 cut(s) 377, 501, 521
Ama87I CYCGRG 2 cut(s) 135, 535
AoxI GGCC 1 cut(s) 891
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 2 cut(s) 275, 553
ApoI RAATTY 1 cut(s) 627
AsiGI ACCGGT 1 cut(s) 371
Asp718I GGTACC 1 cut(s) 444
AspS9I GGNCC 3 cut(s) 245, 825, 891
AsuC2I CCSGG 6 cut(s) 117, 136, 137, 249, 536, 537
AvaI CYCGRG 2 cut(s) 135, 535
AvaII GGWCC 2 cut(s) 245, 825
AxyI CCTNAGG 1 cut(s) 213
BaeGI GKGCMC 1 cut(s) 683
BanI GGYRCC 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 905
Bbv12I GWGCWC 3 cut(s) 262, 683, 836
BbvI GCAGC 2 cut(s) 262, 565
BccI CCATC 2 cut(s) 65, 349
BciT130I CCWGG 1 cut(s) 505
BcnI CCSGG 6 cut(s) 117, 136, 137, 249, 536, 537
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 694
BglII AGATCT 1 cut(s) 583
BisI GCNGC 2 cut(s) 276, 554
BlsI GCNGC 2 cut(s) 277, 555
Bme1390I CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
Bme18I GGWCC 2 cut(s) 245, 825
BmeT110I CYCGRG 2 cut(s) 135, 535
BmgT120I GGNCC 3 cut(s) 245, 825, 891
BmiI GGNNCC 5 cut(s) 128, 246, 247, 446, 892
BmrFI CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
BpiI GAAGAC 1 cut(s) 905
BpmI CTGGAG 2 cut(s) 968, 1021
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 6 cut(s) 117, 136, 137, 249, 536, 537
BsaJI CCNNGG 6 cut(s) 134, 135, 240, 504, 534, 535
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 949
Bse118I RCCGGY 1 cut(s) 371
Bse1I ACTGG 1 cut(s) 913
Bse21I CCTNAGG 1 cut(s) 213
Bse3DI GCAATG 3 cut(s) 726, 753, 805
BseBI CCWGG 1 cut(s) 505
BseDI CCNNGG 6 cut(s) 134, 135, 240, 504, 534, 535
BseGI GGATG 2 cut(s) 165, 616
BseLI CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 949
BseMI GCAATG 3 cut(s) 726, 753, 805
BseNI ACTGG 1 cut(s) 913
BseRI GAGGAG 1 cut(s) 102
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 2 cut(s) 262, 565
BsgI GTGCAG 2 cut(s) 129, 396
BshFI GGCC 1 cut(s) 893
BshNI GGYRCC 1 cut(s) 444
BshTI ACCGGT 1 cut(s) 371
BsiHKAI GWGCWC 3 cut(s) 262, 683, 836
BsiHKCI CYCGRG 2 cut(s) 135, 535
BsiSI CCGG 5 cut(s) 117, 136, 249, 372, 536
BslFI GGGAC 1 cut(s) 231
BslI CCNNNNNNNGG 7 cut(s) 122, 123, 136, 175, 254, 536, 949
BsmFI GGGAC 1 cut(s) 231
BsnI GGCC 1 cut(s) 893
BsoBI CYCGRG 2 cut(s) 135, 535
Bsp1286I GDGCHC 3 cut(s) 262, 683, 836
Bsp143I GATC 4 cut(s) 382, 493, 526, 583
BspACI CCGC 2 cut(s) 173, 944
BspANI GGCC 1 cut(s) 893
BspLI GGNNCC 5 cut(s) 128, 246, 247, 446, 892
BspMAI CTGCAG 1 cut(s) 691
BspMI ACCTGC 1 cut(s) 694
BspPI GGATC 3 cut(s) 377, 501, 521
BspT107I GGYRCC 1 cut(s) 444
BsrDI GCAATG 3 cut(s) 726, 753, 805
BsrFI RCCGGY 1 cut(s) 371
BsrI ACTGG 1 cut(s) 913
BssAI RCCGGY 1 cut(s) 371
BssECI CCNNGG 6 cut(s) 134, 135, 240, 504, 534, 535
BssMI GATC 4 cut(s) 382, 493, 526, 583
BssT1I CCWWGG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 4 cut(s) 338, 576, 807, 972
Bst6I CTCTTC 1 cut(s) 1008
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 1 cut(s) 213
BstF5I GGATG 2 cut(s) 165, 616
BstKTI GATC 4 cut(s) 385, 496, 529, 586
BstMBI GATC 4 cut(s) 382, 493, 526, 583
BstMWI GCNNNNNNNGC 1 cut(s) 899
BstNI CCWGG 1 cut(s) 505
BstSCI CCNGG 7 cut(s) 115, 134, 135, 247, 503, 534, 535
BstSFI CTRYAG 1 cut(s) 687
BstSLI GKGCMC 1 cut(s) 683
BstV1I GCAGC 2 cut(s) 262, 565
BstV2I GAAGAC 1 cut(s) 905
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
Bsu36I CCTNAGG 1 cut(s) 213
BsuRI GGCC 1 cut(s) 893
BtsCI GGATG 2 cut(s) 165, 616
BtsIMutI CAGTG 1 cut(s) 334
BveI ACCTGC 1 cut(s) 694
Cac8I GCNNGC 1 cut(s) 558
Cfr10I RCCGGY 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 245, 825, 891
Cfr9I CCCGGG 2 cut(s) 135, 535
Csp6I GTAC 3 cut(s) 445, 978, 1050
CspAI ACCGGT 1 cut(s) 371
CspCI CAANNNNNGTGG 2 cut(s) 708, 743
CviAII CATG 3 cut(s) 253, 829, 895
CviQI GTAC 3 cut(s) 445, 978, 1050
DdeI CTNAG 1 cut(s) 213
DpnI GATC 4 cut(s) 384, 495, 528, 585
DpnII GATC 4 cut(s) 382, 493, 526, 583
DraIII CACNNNGTG 1 cut(s) 758
Eam1104I CTCTTC 1 cut(s) 1008
EarI CTCTTC 1 cut(s) 1008
Eco130I CCWWGG 1 cut(s) 240
Eco32I GATATC 1 cut(s) 472
Eco47I GGWCC 2 cut(s) 245, 825
Eco81I CCTNAGG 1 cut(s) 213
Eco88I CYCGRG 2 cut(s) 135, 535
EcoO109I RGGNCCY 1 cut(s) 245
EcoRII CCWGG 1 cut(s) 503
EcoRV GATATC 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 240
FaeI CATG 3 cut(s) 256, 832, 898
FalI AAGNNNNNCTT 4 cut(s) 114, 146, 759, 791
FaqI GGGAC 1 cut(s) 231
FatI CATG 3 cut(s) 252, 828, 894
FauI CCCGC 2 cut(s) 180, 951
Fnu4HI GCNGC 2 cut(s) 276, 554
FokI GGATG 2 cut(s) 152, 623
Fsp4HI GCNGC 2 cut(s) 276, 554
FspBI CTAG 1 cut(s) 20
GluI GCNGC 2 cut(s) 276, 554
GsuI CTGGAG 2 cut(s) 968, 1021
HaeIII GGCC 1 cut(s) 893
HapII CCGG 5 cut(s) 117, 136, 249, 372, 536
Hin1II CATG 3 cut(s) 256, 832, 898
HinfI GANTC 3 cut(s) 450, 953, 1016
HpaII CCGG 5 cut(s) 117, 136, 249, 372, 536
Hpy166II GTNNAC 1 cut(s) 681
Hpy188I TCNGA 3 cut(s) 706, 838, 1021
Hpy188III TCNNGA 2 cut(s) 667, 1000
Hpy8I GTNNAC 1 cut(s) 681
HpyAV CCTTC 4 cut(s) 140, 354, 431, 843
HpyCH4III ACNGT 4 cut(s) 338, 576, 807, 972
HpyCH4IV ACGT 2 cut(s) 742, 987
HpyF10VI GCNNNNNNNGC 1 cut(s) 899
HpyF3I CTNAG 1 cut(s) 213
HpySE526I ACGT 2 cut(s) 742, 987
Hsp92II CATG 3 cut(s) 256, 832, 898
KflI GGGWCCC 1 cut(s) 245
KpnI GGTACC 1 cut(s) 448
Kzo9I GATC 4 cut(s) 382, 493, 526, 583
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 2 cut(s) 262, 565
MaeI CTAG 1 cut(s) 20
MaeII ACGT 2 cut(s) 742, 987
MaeIII GTNAC 4 cut(s) 61, 233, 367, 758
MalI GATC 4 cut(s) 384, 495, 528, 585
MboI GATC 4 cut(s) 382, 493, 526, 583
MboII GAAGA 6 cut(s) 16, 322, 578, 905, 1025, 1051
MflI RGATCY 1 cut(s) 583
MhlI GDGCHC 3 cut(s) 262, 683, 836
MluCI AATT 4 cut(s) 78, 219, 423, 627
MlyI GAGTC 1 cut(s) 1025
MmeI TCCRAC 2 cut(s) 184, 460
MnlI CCTC 7 cut(s) 22, 123, 179, 239, 435, 816, 943
MseI TTAA 2 cut(s) 330, 512
MslI CAYNNNNRTG 1 cut(s) 304
MspA1I CMGCKG 3 cut(s) 175, 304, 946
MspI CCGG 5 cut(s) 117, 136, 249, 372, 536
MspR9I CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
MvaI CCWGG 1 cut(s) 505
MwoI GCNNNNNNNGC 1 cut(s) 899
NciI CCSGG 6 cut(s) 117, 136, 137, 249, 536, 537
NdeII GATC 4 cut(s) 382, 493, 526, 583
NlaIII CATG 3 cut(s) 256, 832, 898
NlaIV GGNNCC 5 cut(s) 128, 246, 247, 446, 892
NmuCI GTSAC 3 cut(s) 61, 367, 758
OliI CACNNNNGTG 1 cut(s) 304
PaqCI CACCTGC 1 cut(s) 694
PfeI GAWTC 2 cut(s) 450, 953
PinAI ACCGGT 1 cut(s) 371
PkrI GCNGC 2 cut(s) 277, 555
PleI GAGTC 1 cut(s) 1024
PpsI GAGTC 1 cut(s) 1024
PpuMI RGGWCCY 1 cut(s) 245
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 5 cut(s) 128, 246, 247, 446, 892
PspPI GGNCC 3 cut(s) 245, 825, 891
PspPPI RGGWCCY 1 cut(s) 245
PstI CTGCAG 1 cut(s) 691
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 3 cut(s) 446, 979, 1051
RsaNI GTAC 3 cut(s) 445, 978, 1050
RseI CAYNNNNRTG 1 cut(s) 304
SaqAI TTAA 2 cut(s) 330, 512
SatI GCNGC 2 cut(s) 276, 554
Sau3AI GATC 4 cut(s) 382, 493, 526, 583
Sau96I GGNCC 3 cut(s) 245, 825, 891
SchI GAGTC 1 cut(s) 1025
ScrFI CCNGG 7 cut(s) 117, 136, 137, 249, 505, 536, 537
SduI GDGCHC 3 cut(s) 262, 683, 836
SfcI CTRYAG 1 cut(s) 687
SinI GGWCC 2 cut(s) 245, 825
SmaI CCCGGG 2 cut(s) 137, 537
SmiMI CAYNNNNRTG 1 cut(s) 304
SmlI CTYRAG 1 cut(s) 665
SmoI CTYRAG 1 cut(s) 665
Sse9I AATT 4 cut(s) 78, 219, 423, 627
SsiI CCGC 2 cut(s) 173, 944
SspMI CTAG 1 cut(s) 20
StyD4I CCNGG 7 cut(s) 115, 134, 135, 247, 503, 534, 535
StyI CCWWGG 1 cut(s) 240
TaaI ACNGT 4 cut(s) 338, 576, 807, 972
TaiI ACGT 2 cut(s) 745, 990
TaqI TCGA 1 cut(s) 86
TasI AATT 4 cut(s) 78, 219, 423, 627
TfiI GAWTC 2 cut(s) 450, 953
Tru1I TTAA 2 cut(s) 330, 512
Tru9I TTAA 2 cut(s) 330, 512
TscAI CASTG 1 cut(s) 341
TseFI GTSAC 3 cut(s) 61, 367, 758
TseI GCWGC 2 cut(s) 275, 553
Tsp45I GTSAC 3 cut(s) 61, 367, 758
TspDTI ATGAA 2 cut(s) 17, 816
TspMI CCCGGG 2 cut(s) 135, 535
TspRI CASTG 1 cut(s) 341
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 2 cut(s) 245, 825
XapI RAATTY 1 cut(s) 627
XmaI CCCGGG 2 cut(s) 135, 535
XspI CTAG 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.