Rmu_sc0005711.1_g000004

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005711.1
OG0014229
Physical Location & Seq
Reverse (-)
21543 .. 23330
1788 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005711.1_g000004.1.cds

Sequence Viewer

Length: 1056 bp
atgaagaaacagattgctctagggtttgagggttcagcaaacaagatcggtgttggtgttgtgaccttagatggcacaattttgtcaaacccacgccacacatacattacccctcctggccaaggtttccttccccgtgaaactgcacaacaccatctccaatacattctgccattaattaaatctgctttggaaactgcaaaagtaagccctcaagagatcgattgcctttgttacaccaagggtcctggaatgggagcaccacttcaagttgctgccattgttgttagggttctttcacagctgtggaagaagcccattgttgcagtcaatcattgtgttgctcatattgagatgggaaggattgtgactggtgcggatgatcctgttgtgttgtatgtaagtggtgggaacacacaggtaattgcatatagtgaaggaaggtatcgaatctttggagagaccattgatattgctgttggaaattgcttggatcggtttgctagggtattagaactctccaatgatccagcccccggatataacattgagcagcttgcaaagaaaggagaacagtttatagaccttccttatgctgtcaaagggatggatgtgtcttttagtggaattctgagctacattgaagccactgctgtggagaaattaaaaaatgacgagtgcacccctgcagacctgtgctactcactacaggaaaatgtgttcgcaatgcttgttgagataacagaacgtgcaatggcacattgtgacaagagagatgtacttattgttggtggtgtgggttgcaatgagcgcctgcaagagatgatgaaaaccatgtgtgctgaacggggtggaagattgtttgcaactgatgataggtattgtattgataatggggcaatgattgcttatactggtcttcttgcttttgctcatggaacatcaaccccattggaggaatcgactttcacgcaacggttccggactgatgaagtggaagcaatctggagagtaaaagaggagtcagcaaaagtttcagggtatgggagtagctaa

Protein Analysis

351

Amino Acids

38.36

Weight (kDa)

5.48

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22720 AT4G22720
fragaria_vesca FvH4_3g16550 FvH4_3g16550
malus_domestica MD17G1219400.v1.1
prunus_persica Prupe.1G196300_v2.0.a1
pyrus_communis pycom17g22410
rosa_chinensis RchiOBHm_Chr1g0331181
rosa_laevigata RLG00000029747
rosa_multiflora Rmu_sc0005711.1_g000004
rosa_roxburghii Rroxscaffold_4G00319280
rosa_rugosa Rorug01G0092100
rosa_samantha Rh1AG116100 Rh1BG087800 Rh1CG111600 Rh1DG120500
rosa_wichuraiana Rw1G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 981
AciI CCGC 1 cut(s) 377
AclWI GGATC 3 cut(s) 377, 501, 521
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 627
AdeI CACNNNGTG 1 cut(s) 764
AfaI GTAC 1 cut(s) 780
AfiI CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
AgsI TTSAA 2 cut(s) 269, 644
AjnI CCWGG 2 cut(s) 115, 247
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AleI CACNNNNGTG 2 cut(s) 304, 653
AloI GAACNNNNNNTCC 2 cut(s) 704, 736
AluBI AGCT 4 cut(s) 304, 556, 636, 1053
AluI AGCT 4 cut(s) 304, 556, 636, 1053
Alw21I GWGCWC 2 cut(s) 262, 683
Alw26I GTCTC 1 cut(s) 455
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 3 cut(s) 377, 501, 521
Aor13HI TCCGGA 1 cut(s) 981
AoxI GGCC 1 cut(s) 118
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 2 cut(s) 275, 553
ApoI RAATTY 1 cut(s) 627
AseI ATTAAT 1 cut(s) 176
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 1 cut(s) 245
AsuC2I CCSGG 1 cut(s) 537
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 683
BalI TGGCCA 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 911
Bbv12I GWGCWC 2 cut(s) 262, 683
BbvI GCAGC 2 cut(s) 262, 565
BccI CCATC 4 cut(s) 65, 162, 349, 601
BciT130I CCWGG 2 cut(s) 117, 249
BcnI CCSGG 1 cut(s) 537
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 2 cut(s) 20, 504
BfmI CTRYAG 2 cut(s) 687, 707
BfoI RGCGCY 1 cut(s) 814
BisI GCNGC 2 cut(s) 276, 554
BlsI GCNGC 2 cut(s) 277, 555
Bme1390I CCNGG 3 cut(s) 117, 249, 537
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 2 cut(s) 246, 980
BmrFI CCNGG 3 cut(s) 117, 249, 537
BpiI GAAGAC 1 cut(s) 911
BpmI CTGGAG 1 cut(s) 1027
BpuEI CTTGAG 1 cut(s) 198
BpuMI CCSGG 1 cut(s) 537
Bsa29I ATCGAT 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 3 cut(s) 121, 240, 535
BsaWI WCCGGW 1 cut(s) 981
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
Bse1I ACTGG 2 cut(s) 376, 919
Bse3DI GCAATG 4 cut(s) 732, 759, 811, 906
BseAI TCCGGA 1 cut(s) 981
BseBI CCWGG 2 cut(s) 117, 249
BseCI ATCGAT 1 cut(s) 222
BseDI CCNNGG 3 cut(s) 121, 240, 535
BseGI GGATG 3 cut(s) 385, 612, 616
BseLI CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
BseMI GCAATG 4 cut(s) 732, 759, 811, 906
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 2 cut(s) 376, 919
BseRI GAGGAG 1 cut(s) 1034
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 2 cut(s) 262, 565
BsgI GTGCAG 1 cut(s) 129
BshFI GGCC 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 2 cut(s) 262, 683
BsiSI CCGG 2 cut(s) 537, 982
BslI CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
BsmAI GTCTC 1 cut(s) 455
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 2 cut(s) 262, 683
Bsp13I TCCGGA 1 cut(s) 981
Bsp143I GATC 5 cut(s) 45, 219, 382, 493, 526
BspACI CCGC 1 cut(s) 377
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 624
BspDI ATCGAT 1 cut(s) 222
BspEI TCCGGA 1 cut(s) 981
BspLI GGNNCC 2 cut(s) 246, 980
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 3 cut(s) 377, 501, 521
BspTNI GGTCTC 1 cut(s) 455
BsrDI GCAATG 4 cut(s) 732, 759, 811, 906
BsrI ACTGG 2 cut(s) 376, 919
BssECI CCNNGG 3 cut(s) 121, 240, 535
BssMI GATC 5 cut(s) 45, 219, 382, 493, 526
BssT1I CCWWGG 2 cut(s) 121, 240
Bst2UI CCWGG 2 cut(s) 117, 249
Bst4CI ACNGT 2 cut(s) 576, 978
BstAPI GCANNNNNTGC 1 cut(s) 905
BstC8I GCNNGC 2 cut(s) 558, 815
BstDEI CTNAG 2 cut(s) 67, 632
BstENI CCTNNNNNAGG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 385, 612, 616
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 5 cut(s) 48, 222, 385, 496, 529
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 5 cut(s) 45, 219, 382, 493, 526
BstMWI GCNNNNNNNGC 2 cut(s) 810, 905
BstNI CCWGG 2 cut(s) 117, 249
BstSCI CCNGG 3 cut(s) 115, 247, 535
BstSFI CTRYAG 2 cut(s) 687, 707
BstSLI GKGCMC 1 cut(s) 683
BstV1I GCAGC 2 cut(s) 262, 565
BstV2I GAAGAC 1 cut(s) 911
BstXI CCANNNNNNTGG 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 222
BsuRI GGCC 1 cut(s) 120
BsuTUI ATCGAT 1 cut(s) 222
BtsCI GGATG 3 cut(s) 385, 612, 616
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 2 cut(s) 558, 815
CfoI GCGC 1 cut(s) 813
Cfr13I GGNCC 1 cut(s) 245
ClaI ATCGAT 1 cut(s) 222
Csp6I GTAC 1 cut(s) 779
CviAII CATG 2 cut(s) 835, 935
CviJI RGCY 9 cut(s) 120, 210, 304, 316, 533, 556, 636, 647, 1053
CviKI_1 RGCY 9 cut(s) 120, 210, 304, 316, 533, 556, 636, 647, 1053
CviQI GTAC 1 cut(s) 779
DdeI CTNAG 2 cut(s) 67, 632
DpnI GATC 5 cut(s) 47, 221, 384, 495, 528
DpnII GATC 5 cut(s) 45, 219, 382, 493, 526
DraIII CACNNNGTG 1 cut(s) 764
EaeI YGGCCR 1 cut(s) 118
Eco130I CCWWGG 2 cut(s) 121, 240
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 245
EcoNI CCTNNNNNAGG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 627
EcoRII CCWGG 2 cut(s) 115, 247
EcoT14I CCWWGG 2 cut(s) 121, 240
ErhI CCWWGG 2 cut(s) 121, 240
FaeI CATG 2 cut(s) 838, 938
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 2 cut(s) 834, 934
Fnu4HI GCNGC 2 cut(s) 276, 554
FokI GGATG 3 cut(s) 392, 619, 623
Fsp4HI GCNGC 2 cut(s) 276, 554
FspBI CTAG 2 cut(s) 20, 504
GlaI GCGC 1 cut(s) 812
GluI GCNGC 2 cut(s) 276, 554
GsuI CTGGAG 1 cut(s) 1027
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 1 cut(s) 120
HapII CCGG 2 cut(s) 537, 982
HhaI GCGC 1 cut(s) 813
Hin1II CATG 2 cut(s) 838, 938
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HinfI GANTC 3 cut(s) 450, 959, 1022
HpaII CCGG 2 cut(s) 537, 982
Hpy166II GTNNAC 1 cut(s) 681
Hpy188I TCNGA 1 cut(s) 633
Hpy188III TCNNGA 3 cut(s) 215, 982, 1006
Hpy8I GTNNAC 1 cut(s) 681
HpyAV CCTTC 5 cut(s) 140, 354, 431, 435, 596
HpyCH4III ACNGT 2 cut(s) 576, 978
HpyCH4IV ACGT 1 cut(s) 748
HpyF10VI GCNNNNNNNGC 2 cut(s) 810, 905
HpyF3I CTNAG 2 cut(s) 67, 632
HpySE526I ACGT 1 cut(s) 748
Hsp92II CATG 2 cut(s) 838, 938
HspAI GCGC 1 cut(s) 811
Kpn2I TCCGGA 1 cut(s) 981
Kzo9I GATC 5 cut(s) 45, 219, 382, 493, 526
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 2 cut(s) 262, 565
MaeI CTAG 2 cut(s) 20, 504
MaeII ACGT 1 cut(s) 748
MaeIII GTNAC 4 cut(s) 61, 233, 367, 764
MalI GATC 5 cut(s) 47, 221, 384, 495, 528
MboI GATC 5 cut(s) 45, 219, 382, 493, 526
MboII GAAGA 4 cut(s) 16, 322, 867, 911
MhlI GDGCHC 2 cut(s) 262, 683
MlsI TGGCCA 1 cut(s) 120
MluCI AATT 6 cut(s) 78, 177, 423, 484, 627, 662
MluNI TGGCCA 1 cut(s) 120
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 5 cut(s) 22, 123, 222, 949, 1012
Mox20I TGGCCA 1 cut(s) 120
MroI TCCGGA 1 cut(s) 981
MscI TGGCCA 1 cut(s) 120
MseI TTAA 3 cut(s) 176, 180, 665
MslI CAYNNNNRTG 2 cut(s) 304, 653
Msp20I TGGCCA 1 cut(s) 120
MspA1I CMGCKG 1 cut(s) 304
MspI CCGG 2 cut(s) 537, 982
MspR9I CCNGG 3 cut(s) 117, 249, 537
MvaI CCWGG 2 cut(s) 117, 249
MwoI GCNNNNNNNGC 2 cut(s) 810, 905
NciI CCSGG 1 cut(s) 537
NdeII GATC 5 cut(s) 45, 219, 382, 493, 526
NlaIII CATG 2 cut(s) 838, 938
NlaIV GGNNCC 2 cut(s) 246, 980
NmuCI GTSAC 3 cut(s) 61, 367, 764
OliI CACNNNNGTG 2 cut(s) 304, 653
PacI TTAATTAA 1 cut(s) 180
PfeI GAWTC 2 cut(s) 450, 959
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 2 cut(s) 277, 555
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
PpuMI RGGWCCY 1 cut(s) 245
PshBI ATTAAT 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 2 cut(s) 115, 247
PspGI CCWGG 2 cut(s) 115, 247
PspN4I GGNNCC 2 cut(s) 246, 980
PspPI GGNCC 1 cut(s) 245
PspPPI RGGWCCY 1 cut(s) 245
PstI CTGCAG 1 cut(s) 691
PvuII CAGCTG 1 cut(s) 304
RsaI GTAC 1 cut(s) 780
RsaNI GTAC 1 cut(s) 779
RseI CAYNNNNRTG 2 cut(s) 304, 653
SaqAI TTAA 3 cut(s) 176, 180, 665
SatI GCNGC 2 cut(s) 276, 554
Sau3AI GATC 5 cut(s) 45, 219, 382, 493, 526
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 3 cut(s) 117, 249, 537
SduI GDGCHC 2 cut(s) 262, 683
SfcI CTRYAG 2 cut(s) 687, 707
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 304, 653
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse9I AATT 6 cut(s) 78, 177, 423, 484, 627, 662
SsiI CCGC 1 cut(s) 377
SspMI CTAG 2 cut(s) 20, 504
StyD4I CCNGG 3 cut(s) 115, 247, 535
StyI CCWWGG 2 cut(s) 121, 240
TaaI ACNGT 2 cut(s) 576, 978
TaiI ACGT 1 cut(s) 751
TaqI TCGA 3 cut(s) 222, 448, 962
TasI AATT 6 cut(s) 78, 177, 423, 484, 627, 662
TatI WGTACW 1 cut(s) 778
TfiI GAWTC 2 cut(s) 450, 959
Tru1I TTAA 3 cut(s) 176, 180, 665
Tru9I TTAA 3 cut(s) 176, 180, 665
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 3 cut(s) 61, 367, 764
TseI GCWGC 2 cut(s) 275, 553
Tsp45I GTSAC 3 cut(s) 61, 367, 764
TspDTI ATGAA 3 cut(s) 17, 842, 1005
TspRI CASTG 1 cut(s) 655
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 1 cut(s) 245
VspI ATTAAT 1 cut(s) 176
XagI CCTNNNNNAGG 1 cut(s) 120
XapI RAATTY 1 cut(s) 627
XspI CTAG 2 cut(s) 20, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.