Rroxscaffold_4G00319280

Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014229
Physical Location & Seq
Forward (+)
46915527 .. 46922171
6645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00319280.1

Sequence Viewer

Length: 1044 bp
ATGAAGAAACAGATTGCTCTAGGGTTTGAGGGTTCAGCAAACAAGATCGGTGTCGGTGTTGTGACCTTAGATGGCACAATTTTGTCAAACCCACGCCACACATACATTACCCCTCCTGGCCAAGGTTTTCTTCCCCGTGAAACTGCACAACACCATCTCCAATACATTCTGCCATTAATTAAATCTGCTTTGGAAATGGCACAAGTAACTCCTCAAGAGATCGATTGTCTTTGTTACACCAAGGGTCCTGGAATGGGAGCACCGCTACAAGTTGCTGCCATTGTTGTTAGGGTTCTTTCACAGTTGTGGAAGAAGCCCATTGTTGCAGTCAATCATTGTGTTGCTCATATCGAGATGGGAAGGATTGTGACTGGCGCGGATGATCCTGTTGTGTTGTATGTAAGTGGTGGGAACACACAGGTAATTGCATATAGTGAAGGGAGGTATCGAATCTTTGGAGAGACCATTGATATTGCTGTTGGAAATTGCTTGGATCGGTTTGCTAGGGTATTAGAACTCTCCAATGATCCAGCCCCTGGATATAACATTGAGCAGCTTGCAAAGAAAGGAGAACAGTTTATAGACCTTCCTTATGCTGTCAAAGGGATGGATGTGTCTTTTAGTGGAATTCTGAGCTACATTGAAGCCACTGCTGTGGAGAAACTAAAAAATGACGAGTGCACCCCTGCAGACCTGTGCTACTCACTTCAGGAACATGTGTTTGCAATGCTTGTTGAGATAACAGAACGTGCAATGGCACATTGTGACAAGAGAGATGTGCTTATTGTTGGTGGTGTTGGTTGCAATGAGCGCCTGCAGGAGATGATGAAAACCATGTGTGCTGAACGGGGCGGAAGATTGTTTGCAACTGATGATAGGTATTGTATTGATAATGGGGCAATGATTGCTTATACTGGTCTTCTTGCTTTTGCTCACGGAACATCAACCCCATTGGAGGAATCGACTTTCACGCAACGGTTTCGGACTGATGAAGTGGAAGCAATCTGGAGAGTAAAAGAGGAGTCAGCAAAAGTTAAAGGGTAA

Protein Analysis

347

Amino Acids

38.08

Weight (kDa)

5.57

Isoelectric Point (pI)

30.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TsaD PF00814 32 - 304 1.3e-84 tRNA N6-adenosine threonylcarbamoyltransferase
HypF_C_2 PF22521 178 - 297 2.5e-06 Carbamoyltransferase, Kae1-like Domain, second subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014229)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G22720 AT4G22720
fragaria_vesca FvH4_3g16550 FvH4_3g16550
malus_domestica MD17G1219400.v1.1
prunus_persica Prupe.1G196300_v2.0.a1
pyrus_communis pycom17g22410
rosa_chinensis RchiOBHm_Chr1g0331181
rosa_laevigata RLG00000029747
rosa_multiflora Rmu_sc0005711.1_g000004
rosa_roxburghii Rroxscaffold_4G00319280
rosa_rugosa Rorug01G0092100
rosa_samantha Rh1AG116100 Rh1BG087800 Rh1CG111600 Rh1DG120500
rosa_wichuraiana Rw1G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 536
AccII CGCG 1 cut(s) 377
AciI CCGC 3 cut(s) 263, 377, 852
AclWI GGATC 3 cut(s) 377, 501, 521
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 1 cut(s) 627
AcuI CTGAAG 1 cut(s) 692
AdeI CACNNNGTG 1 cut(s) 764
AfiI CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
AflIII ACRYGT 1 cut(s) 715
AgsI TTSAA 1 cut(s) 644
AjnI CCWGG 3 cut(s) 115, 247, 535
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AleI CACNNNNGTG 2 cut(s) 304, 653
AluBI AGCT 2 cut(s) 556, 636
AluI AGCT 2 cut(s) 556, 636
Alw21I GWGCWC 2 cut(s) 262, 683
Alw26I GTCTC 1 cut(s) 455
Alw44I GTGCAC 1 cut(s) 679
AlwI GGATC 3 cut(s) 377, 501, 521
AlwNI CAGNNNCTG 1 cut(s) 536
AoxI GGCC 1 cut(s) 118
ApaLI GTGCAC 1 cut(s) 679
ApeKI GCWGC 2 cut(s) 275, 553
ApoI RAATTY 1 cut(s) 627
AseI ATTAAT 1 cut(s) 176
AspLEI GCGC 2 cut(s) 377, 813
AspS9I GGNCC 1 cut(s) 245
AvaII GGWCC 1 cut(s) 245
BaeGI GKGCMC 1 cut(s) 683
BalI TGGCCA 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 911
Bbv12I GWGCWC 2 cut(s) 262, 683
BbvI GCAGC 2 cut(s) 262, 565
BccI CCATC 4 cut(s) 65, 162, 349, 601
BcgI CGANNNNNNTGC 2 cut(s) 962, 996
BciT130I CCWGG 3 cut(s) 117, 249, 537
BcoDI GTCTC 1 cut(s) 455
BfaI CTAG 2 cut(s) 20, 504
BfmI CTRYAG 2 cut(s) 687, 815
BfoI RGCGCY 1 cut(s) 814
BisI GCNGC 2 cut(s) 276, 554
BlsI GCNGC 2 cut(s) 277, 555
Bme1390I CCNGG 3 cut(s) 117, 249, 537
Bme18I GGWCC 1 cut(s) 245
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 3 cut(s) 117, 249, 537
BpiI GAAGAC 1 cut(s) 911
BpmI CTGGAG 1 cut(s) 1027
BpuEI CTTGAG 1 cut(s) 198
Bsa29I ATCGAT 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 455
BsaJI CCNNGG 3 cut(s) 121, 240, 535
BsaXI ACNNNNNCTCC 2 cut(s) 141, 171
Bsc4I CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
Bse1I ACTGG 2 cut(s) 376, 919
Bse3DI GCAATG 4 cut(s) 732, 759, 811, 906
BseBI CCWGG 3 cut(s) 117, 249, 537
BseCI ATCGAT 1 cut(s) 222
BseDI CCNNGG 3 cut(s) 121, 240, 535
BseGI GGATG 3 cut(s) 385, 612, 616
BseLI CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
BseMI GCAATG 4 cut(s) 732, 759, 811, 906
BseMII CTCAG 1 cut(s) 623
BseNI ACTGG 2 cut(s) 376, 919
BseRI GAGGAG 2 cut(s) 201, 1034
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 2 cut(s) 262, 565
BsgI GTGCAG 1 cut(s) 129
Bsh1236I CGCG 1 cut(s) 377
BshFI GGCC 1 cut(s) 120
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 2 cut(s) 262, 683
BslI CCNNNNNNNGG 4 cut(s) 122, 254, 536, 955
BsmAI GTCTC 1 cut(s) 455
BsnI GGCC 1 cut(s) 120
Bso31I GGTCTC 1 cut(s) 455
Bsp1286I GDGCHC 2 cut(s) 262, 683
Bsp143I GATC 5 cut(s) 45, 219, 382, 493, 526
BspACI CCGC 3 cut(s) 263, 377, 852
BspANI GGCC 1 cut(s) 120
BspCNI CTCAG 1 cut(s) 624
BspDI ATCGAT 1 cut(s) 222
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 1 cut(s) 246
BspMAI CTGCAG 2 cut(s) 691, 819
BspPI GGATC 3 cut(s) 377, 501, 521
BspTNI GGTCTC 1 cut(s) 455
BsrDI GCAATG 4 cut(s) 732, 759, 811, 906
BsrI ACTGG 2 cut(s) 376, 919
BssECI CCNNGG 3 cut(s) 121, 240, 535
BssMI GATC 5 cut(s) 45, 219, 382, 493, 526
BssT1I CCWWGG 2 cut(s) 121, 240
Bst2UI CCWGG 3 cut(s) 117, 249, 537
Bst4CI ACNGT 3 cut(s) 303, 576, 978
BstAPI GCANNNNNTGC 1 cut(s) 905
BstC8I GCNNGC 2 cut(s) 558, 815
BstDEI CTNAG 2 cut(s) 67, 632
BstENI CCTNNNNNAGG 1 cut(s) 120
BstF5I GGATG 3 cut(s) 385, 612, 616
BstFNI CGCG 1 cut(s) 377
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 2 cut(s) 377, 813
BstKTI GATC 5 cut(s) 48, 222, 385, 496, 529
BstMAI GTCTC 1 cut(s) 455
BstMBI GATC 5 cut(s) 45, 219, 382, 493, 526
BstMWI GCNNNNNNNGC 2 cut(s) 810, 905
BstNI CCWGG 3 cut(s) 117, 249, 537
BstNSI RCATGY 1 cut(s) 719
BstSCI CCNGG 3 cut(s) 115, 247, 535
BstSFI CTRYAG 2 cut(s) 687, 815
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 2 cut(s) 262, 565
BstV2I GAAGAC 1 cut(s) 911
BstXI CCANNNNNNTGG 1 cut(s) 655
Bsu15I ATCGAT 1 cut(s) 222
BsuRI GGCC 1 cut(s) 120
BsuTUI ATCGAT 1 cut(s) 222
BtsCI GGATG 3 cut(s) 385, 612, 616
BtsI GCAGTG 1 cut(s) 648
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 2 cut(s) 558, 815
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 2 cut(s) 377, 813
Cfr13I GGNCC 1 cut(s) 245
ClaI ATCGAT 1 cut(s) 222
CviAII CATG 2 cut(s) 716, 835
CviJI RGCY 6 cut(s) 120, 316, 533, 556, 636, 647
CviKI_1 RGCY 6 cut(s) 120, 316, 533, 556, 636, 647
DdeI CTNAG 2 cut(s) 67, 632
DpnI GATC 5 cut(s) 47, 221, 384, 495, 528
DpnII GATC 5 cut(s) 45, 219, 382, 493, 526
DraIII CACNNNGTG 1 cut(s) 764
EaeI YGGCCR 1 cut(s) 118
EciI GGCGGA 1 cut(s) 867
Eco130I CCWWGG 2 cut(s) 121, 240
Eco31I GGTCTC 1 cut(s) 455
Eco47I GGWCC 1 cut(s) 245
Eco57I CTGAAG 1 cut(s) 692
EcoNI CCTNNNNNAGG 1 cut(s) 120
EcoO109I RGGNCCY 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 627
EcoRII CCWGG 3 cut(s) 115, 247, 535
EcoT14I CCWWGG 2 cut(s) 121, 240
ErhI CCWWGG 2 cut(s) 121, 240
FaeI CATG 2 cut(s) 719, 838
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 2 cut(s) 715, 834
Fnu4HI GCNGC 2 cut(s) 276, 554
FokI GGATG 3 cut(s) 392, 619, 623
Fsp4HI GCNGC 2 cut(s) 276, 554
FspBI CTAG 2 cut(s) 20, 504
GlaI GCGC 2 cut(s) 376, 812
GluI GCNGC 2 cut(s) 276, 554
GsuI CTGGAG 1 cut(s) 1027
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 1 cut(s) 120
HhaI GCGC 2 cut(s) 377, 813
Hin1II CATG 2 cut(s) 719, 838
Hin6I GCGC 2 cut(s) 375, 811
HinP1I GCGC 2 cut(s) 375, 811
HinfI GANTC 3 cut(s) 450, 959, 1022
Hpy166II GTNNAC 1 cut(s) 681
Hpy188I TCNGA 2 cut(s) 633, 984
Hpy188III TCNNGA 4 cut(s) 215, 352, 710, 1006
Hpy8I GTNNAC 1 cut(s) 681
HpyAV CCTTC 3 cut(s) 354, 431, 596
HpyCH4III ACNGT 3 cut(s) 303, 576, 978
HpyCH4IV ACGT 1 cut(s) 748
HpyF10VI GCNNNNNNNGC 2 cut(s) 810, 905
HpyF3I CTNAG 2 cut(s) 67, 632
HpySE526I ACGT 1 cut(s) 748
Hsp92II CATG 2 cut(s) 719, 838
HspAI GCGC 2 cut(s) 375, 811
Kzo9I GATC 5 cut(s) 45, 219, 382, 493, 526
LmnI GCTCC 1 cut(s) 257
Lsp1109I GCAGC 2 cut(s) 262, 565
MaeI CTAG 2 cut(s) 20, 504
MaeII ACGT 1 cut(s) 748
MaeIII GTNAC 5 cut(s) 61, 205, 233, 367, 764
MalI GATC 5 cut(s) 47, 221, 384, 495, 528
MboI GATC 5 cut(s) 45, 219, 382, 493, 526
MboII GAAGA 5 cut(s) 16, 122, 322, 867, 911
MhlI GDGCHC 2 cut(s) 262, 683
MlsI TGGCCA 1 cut(s) 120
MluCI AATT 5 cut(s) 78, 177, 423, 484, 627
MluNI TGGCCA 1 cut(s) 120
MlyI GAGTC 1 cut(s) 1031
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 6 cut(s) 22, 123, 222, 435, 949, 1012
Mox20I TGGCCA 1 cut(s) 120
MscI TGGCCA 1 cut(s) 120
MseI TTAA 3 cut(s) 176, 180, 1035
MslI CAYNNNNRTG 2 cut(s) 304, 653
Msp20I TGGCCA 1 cut(s) 120
MspR9I CCNGG 3 cut(s) 117, 249, 537
MvaI CCWGG 3 cut(s) 117, 249, 537
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 2 cut(s) 810, 905
NdeII GATC 5 cut(s) 45, 219, 382, 493, 526
NlaIII CATG 2 cut(s) 719, 838
NlaIV GGNNCC 1 cut(s) 246
NmuCI GTSAC 3 cut(s) 61, 367, 764
NspI RCATGY 1 cut(s) 719
OliI CACNNNNGTG 2 cut(s) 304, 653
PacI TTAATTAA 1 cut(s) 180
PciI ACATGT 1 cut(s) 715
PfeI GAWTC 2 cut(s) 450, 959
PflMI CCANNNNNTGG 1 cut(s) 536
PfoI TCCNGGA 1 cut(s) 247
PkrI GCNGC 2 cut(s) 277, 555
PleI GAGTC 1 cut(s) 1030
PpsI GAGTC 1 cut(s) 1030
PpuMI RGGWCCY 1 cut(s) 245
PscI ACATGT 1 cut(s) 715
PshBI ATTAAT 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 3 cut(s) 115, 247, 535
PspGI CCWGG 3 cut(s) 115, 247, 535
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 1 cut(s) 245
PspPPI RGGWCCY 1 cut(s) 245
PstI CTGCAG 2 cut(s) 691, 819
PstNI CAGNNNCTG 1 cut(s) 536
RseI CAYNNNNRTG 2 cut(s) 304, 653
SaqAI TTAA 3 cut(s) 176, 180, 1035
SatI GCNGC 2 cut(s) 276, 554
Sau3AI GATC 5 cut(s) 45, 219, 382, 493, 526
Sau96I GGNCC 1 cut(s) 245
SbfI CCTGCAGG 1 cut(s) 819
SchI GAGTC 1 cut(s) 1031
ScrFI CCNGG 3 cut(s) 117, 249, 537
SdaI CCTGCAGG 1 cut(s) 819
SduI GDGCHC 2 cut(s) 262, 683
SfcI CTRYAG 2 cut(s) 687, 815
SinI GGWCC 1 cut(s) 245
SmiMI CAYNNNNRTG 2 cut(s) 304, 653
SmlI CTYRAG 1 cut(s) 213
SmoI CTYRAG 1 cut(s) 213
Sse8387I CCTGCAGG 1 cut(s) 819
Sse9I AATT 5 cut(s) 78, 177, 423, 484, 627
SsiI CCGC 3 cut(s) 263, 377, 852
SspMI CTAG 2 cut(s) 20, 504
StyD4I CCNGG 3 cut(s) 115, 247, 535
StyI CCWWGG 2 cut(s) 121, 240
TaaI ACNGT 3 cut(s) 303, 576, 978
TaiI ACGT 1 cut(s) 751
TaqI TCGA 4 cut(s) 222, 351, 448, 962
TasI AATT 5 cut(s) 78, 177, 423, 484, 627
TfiI GAWTC 2 cut(s) 450, 959
Tru1I TTAA 3 cut(s) 176, 180, 1035
Tru9I TTAA 3 cut(s) 176, 180, 1035
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 3 cut(s) 61, 367, 764
TseI GCWGC 2 cut(s) 275, 553
Tsp45I GTSAC 3 cut(s) 61, 367, 764
TspDTI ATGAA 3 cut(s) 17, 842, 1005
TspGWI ACGGA 1 cut(s) 951
TspRI CASTG 1 cut(s) 655
Van91I CCANNNNNTGG 1 cut(s) 536
VneI GTGCAC 1 cut(s) 679
VpaK11BI GGWCC 1 cut(s) 245
VspI ATTAAT 1 cut(s) 176
XagI CCTNNNNNAGG 1 cut(s) 120
XapI RAATTY 1 cut(s) 627
XceI RCATGY 1 cut(s) 719
XspI CTAG 2 cut(s) 20, 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.