pycom14g08400

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0023604
Physical Location & Seq
Reverse (-)
9133308 .. 9134369
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g08400.1

Sequence Viewer

Length: 516 bp
ATGTTTGATAACCAAAATCAATTTTTCGACGCACCGTGGATGTCACATCCCGGGCCGGGATTTGACAATGGAAGCAATCGACTTTTAACCAATATACCCAAAAAGTTTCCTCACCCAATATCGATCCTTCCATACCATACCATACCATACACCTCTCTTTGCCTCACACACGACCAATATCTTCCTTCAGTTGTTGAAGAATCAAGAGAGAGAGTATATGCGGCCATGAAATATTATTGGGGTAAGAGAAGGAAGCAGCAGATAAGAGTGAGAACAAGAGAGGTGGCTTGCTTGTCTCTGCTGGTGTTGATGCTGCTTCAGTTGGAGACTCCAAGTTTTGCAGCGGCAGCAGCAGCAGCTGGATATAACAATTATAAGGTAAAGTCCAGTACTAGCAGATTCAAAGCAATCCCAACAACAACAAGGATCGATCATAAAGTGGGTGATCATGATGCCGAAATCTTTGGCGTTGATAAGAGGACCATCTATACTGGCCCAAATCCTCTCCACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

19.53

Weight (kDa)

9.49

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0023604)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12235
prunus_persica Prupe.7G031000_v2.0.a1
pyrus_communis pycom14g08400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AciI CCGC 2 cut(s) 221, 344
AclWI GGATC 2 cut(s) 118, 434
AcoI YGGCCR 1 cut(s) 222
AcuI CTGAAG 2 cut(s) 171, 302
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 1 cut(s) 56
AgsI TTSAA 2 cut(s) 197, 403
AluBI AGCT 1 cut(s) 359
AluI AGCT 1 cut(s) 359
Alw26I GTCTC 2 cut(s) 300, 320
AlwI GGATC 2 cut(s) 118, 434
AlwNI CAGNNNCTG 1 cut(s) 359
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 3 cut(s) 53, 222, 493
ApeKI GCWGC 7 cut(s) 256, 313, 341, 347, 350, 353, 356
AspS9I GGNCC 3 cut(s) 53, 480, 494
AsuC2I CCSGG 3 cut(s) 51, 52, 57
AsuHPI GGTGA 2 cut(s) 104, 455
AvaI CYCGRG 1 cut(s) 50
AvaII GGWCC 1 cut(s) 480
BbvI GCAGC 7 cut(s) 268, 300, 353, 359, 362, 365, 368
BccI CCATC 1 cut(s) 491
BclI TGATCA 1 cut(s) 445
BcnI CCSGG 3 cut(s) 51, 52, 57
BcoDI GTCTC 2 cut(s) 300, 320
BfaI CTAG 1 cut(s) 393
BisI GCNGC 9 cut(s) 222, 257, 314, 342, 345, 348, 351, 354, 357
BlsI GCNGC 9 cut(s) 223, 258, 315, 343, 346, 349, 352, 355, 358
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 3 cut(s) 51, 52, 57
Bme18I GGWCC 1 cut(s) 480
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 3 cut(s) 53, 480, 494
BmrFI CCNGG 3 cut(s) 51, 52, 57
BmsI GCATC 2 cut(s) 300, 442
BpuMI CCSGG 3 cut(s) 51, 52, 57
Bsa29I ATCGAT 2 cut(s) 122, 429
BsaJI CCNNGG 2 cut(s) 35, 50
Bsc4I CCNNNNNNNGG 1 cut(s) 56
Bse1I ACTGG 2 cut(s) 387, 496
BseCI ATCGAT 2 cut(s) 122, 429
BseDI CCNNGG 2 cut(s) 35, 50
BseGI GGATG 2 cut(s) 45, 46
BseLI CCNNNNNNNGG 1 cut(s) 56
BseNI ACTGG 2 cut(s) 387, 496
BseXI GCAGC 7 cut(s) 268, 300, 353, 359, 362, 365, 368
BshFI GGCC 3 cut(s) 55, 224, 495
BshVI ATCGAT 2 cut(s) 122, 429
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 2 cut(s) 51, 56
BslI CCNNNNNNNGG 1 cut(s) 56
BsmAI GTCTC 2 cut(s) 300, 320
BsnI GGCC 3 cut(s) 55, 224, 495
BsoBI CYCGRG 1 cut(s) 50
Bsp143I GATC 4 cut(s) 123, 426, 430, 445
BspACI CCGC 2 cut(s) 221, 344
BspANI GGCC 3 cut(s) 55, 224, 495
BspDI ATCGAT 2 cut(s) 122, 429
BspHI TCATGA 1 cut(s) 448
BspPI GGATC 2 cut(s) 118, 434
BsrI ACTGG 2 cut(s) 387, 496
BssECI CCNNGG 2 cut(s) 35, 50
BssMI GATC 4 cut(s) 123, 426, 430, 445
Bst4CI ACNGT 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 289
BstDSI CCRYGG 1 cut(s) 35
BstF5I GGATG 2 cut(s) 45, 46
BstKTI GATC 4 cut(s) 126, 429, 433, 448
BstMAI GTCTC 2 cut(s) 300, 320
BstMBI GATC 4 cut(s) 123, 426, 430, 445
BstMWI GCNNNNNNNGC 4 cut(s) 347, 350, 353, 356
BstSCI CCNGG 3 cut(s) 49, 50, 55
BstV1I GCAGC 7 cut(s) 268, 300, 353, 359, 362, 365, 368
Bsu15I ATCGAT 2 cut(s) 122, 429
BsuRI GGCC 3 cut(s) 55, 224, 495
BsuTUI ATCGAT 2 cut(s) 122, 429
BtgI CCRYGG 1 cut(s) 35
BtsCI GGATG 2 cut(s) 45, 46
Cac8I GCNNGC 1 cut(s) 289
CaiI CAGNNNCTG 1 cut(s) 359
CciI TCATGA 1 cut(s) 448
Cfr13I GGNCC 3 cut(s) 53, 480, 494
Cfr9I CCCGGG 1 cut(s) 50
ClaI ATCGAT 2 cut(s) 122, 429
CseI GACGC 1 cut(s) 38
Csp6I GTAC 1 cut(s) 390
CspCI CAANNNNNGTGG 2 cut(s) 264, 299
CviAII CATG 2 cut(s) 226, 449
CviJI RGCY 5 cut(s) 55, 224, 287, 359, 495
CviKI_1 RGCY 5 cut(s) 55, 224, 287, 359, 495
CviQI GTAC 1 cut(s) 390
DpnI GATC 4 cut(s) 125, 428, 432, 447
DpnII GATC 4 cut(s) 123, 426, 430, 445
EaeI YGGCCR 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 480
Eco57I CTGAAG 2 cut(s) 171, 302
Eco88I CYCGRG 1 cut(s) 50
FaeI CATG 2 cut(s) 229, 452
FatI CATG 2 cut(s) 225, 448
FbaI TGATCA 1 cut(s) 445
Fnu4HI GCNGC 9 cut(s) 222, 257, 314, 342, 345, 348, 351, 354, 357
FokI GGATG 2 cut(s) 33, 52
Fsp4HI GCNGC 9 cut(s) 222, 257, 314, 342, 345, 348, 351, 354, 357
FspBI CTAG 1 cut(s) 393
GluI GCNGC 9 cut(s) 222, 257, 314, 342, 345, 348, 351, 354, 357
HaeIII GGCC 3 cut(s) 55, 224, 495
HapII CCGG 2 cut(s) 51, 56
HgaI GACGC 1 cut(s) 38
Hin1II CATG 2 cut(s) 229, 452
HinfI GANTC 3 cut(s) 200, 328, 399
HpaII CCGG 2 cut(s) 51, 56
HphI GGTGA 2 cut(s) 104, 455
Hpy188III TCNNGA 2 cut(s) 204, 449
Hpy99I CGWCG 1 cut(s) 32
HpyAV CCTTC 3 cut(s) 137, 195, 243
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 1 cut(s) 341
HpyF10VI GCNNNNNNNGC 4 cut(s) 347, 350, 353, 356
Hsp92II CATG 2 cut(s) 229, 452
Ksp22I TGATCA 1 cut(s) 445
Kzo9I GATC 4 cut(s) 123, 426, 430, 445
LpnPI CCDG 6 cut(s) 64, 69, 287, 345, 400, 477
Lsp1109I GCAGC 7 cut(s) 268, 300, 353, 359, 362, 365, 368
LweI GCATC 2 cut(s) 300, 442
MaeI CTAG 1 cut(s) 393
MaeIII GTNAC 1 cut(s) 42
MalI GATC 4 cut(s) 125, 428, 432, 447
MboI GATC 4 cut(s) 123, 426, 430, 445
MboII GAAGA 2 cut(s) 173, 209
MluCI AATT 3 cut(s) 20, 370, 511
MlyI GAGTC 1 cut(s) 322
MmeI TCCRAC 1 cut(s) 303
MnlI CCTC 6 cut(s) 120, 163, 173, 274, 471, 513
MseI TTAA 1 cut(s) 86
MspA1I CMGCKG 2 cut(s) 344, 359
MspI CCGG 2 cut(s) 51, 56
MspR9I CCNGG 3 cut(s) 51, 52, 57
MwoI GCNNNNNNNGC 4 cut(s) 347, 350, 353, 356
NciI CCSGG 3 cut(s) 51, 52, 57
NdeII GATC 4 cut(s) 123, 426, 430, 445
NlaIII CATG 2 cut(s) 229, 452
NmuCI GTSAC 1 cut(s) 42
PagI TCATGA 1 cut(s) 448
PfeI GAWTC 2 cut(s) 200, 399
PkrI GCNGC 9 cut(s) 223, 258, 315, 343, 346, 349, 352, 355, 358
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PsiI TTATAA 1 cut(s) 375
PspPI GGNCC 3 cut(s) 53, 480, 494
PstNI CAGNNNCTG 1 cut(s) 359
PvuII CAGCTG 1 cut(s) 359
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
SaqAI TTAA 1 cut(s) 86
SatI GCNGC 9 cut(s) 222, 257, 314, 342, 345, 348, 351, 354, 357
Sau3AI GATC 4 cut(s) 123, 426, 430, 445
Sau96I GGNCC 3 cut(s) 53, 480, 494
ScaI AGTACT 1 cut(s) 391
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 3 cut(s) 51, 52, 57
SetI ASST 4 cut(s) 155, 285, 361, 381
SfaNI GCATC 2 cut(s) 300, 442
SinI GGWCC 1 cut(s) 480
SmaI CCCGGG 1 cut(s) 52
Sse9I AATT 3 cut(s) 20, 370, 511
SsiI CCGC 2 cut(s) 221, 344
SspI AATATT 1 cut(s) 233
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 3 cut(s) 49, 50, 55
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 4 cut(s) 27, 79, 122, 429
TasI AATT 3 cut(s) 20, 370, 511
TatI WGTACW 1 cut(s) 389
TauI GCSGC 2 cut(s) 224, 347
TfiI GAWTC 2 cut(s) 200, 399
Tru1I TTAA 1 cut(s) 86
Tru9I TTAA 1 cut(s) 86
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 7 cut(s) 256, 313, 341, 347, 350, 353, 356
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 1 cut(s) 242
TspMI CCCGGG 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 480
XmaI CCCGGG 1 cut(s) 50
XspI CTAG 1 cut(s) 393
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.