pycom15g06280
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0022342
Physical Location & Seq
Forward (+)
3848911 .. 3850555
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1119 bp
ATGACACTTACTAACGTTATCAGAAAGCTCAGTCAATTTGGTTGCTCACTCTTCGAATTGATGATTATCAGTGCTGGAAAATTAAGTGGTCATATACCTTACCGAGACTCAAAGCTCACTCGCATATTGCAACATTCCCTTGGAGGAAATGCTCGCACTGCCATCATATGTACCTTGAGTCCAGCACTGAGCCACGCTGAACAATCTCGAAACACTCTCTTCTTTGCTACACAGGCAAAGGAGGTAACAAATGATGCCAGCGTGAACACGGTTGTATCAGACAAGCAGCTAGTGAAACACTTGCAAAAGCAAGTAGCTAAGCTGGAAGCAGAGCTGTGCACCCTTTATCCATTGAGGGAAAGAATTCAGCAGATGGAAATGGAAATGGAGGATTTGAGAAGTGAAAGAGATTTGGCTCAATCTCAAGCGGATGAGTTGCGCCATAAACTTGAGGTTCAGAGTTTATATCCATTGGAATTACCTCATCGCTCTGTGGACAACTATCTCTCCTACACTGATGTGCTACCAACATCATTTTATGCCAAGGAACTAGGAGATTGTGACACAGCAACAAATGCAAGGACCAATCACTCCATTAGGCACTCGTCAACTCCTCCTTTCATGCCAAGACGTGAAGTTCTAAAACATGAAATCTCATCAGAGCAACTTTCGGAGGAACCAAGTCGAGCTCTAAAACTAAAAGTGCTTTACAAAAGCAAATTGGTGATTCCTCCTTGCATTCAAGATTTAGCTTTCAGGTGCTTGGAGGATTTATTTCGTCCTCAAATAAACTCTCTCTTACTTTCCACACAGGCAAAGGGTGAATCTAACATGGTACTACTTTTTTGCTCATCACGTTTTTCAGTTTGCATTTTTACATACTCAGAATACAAACCTATTAATATATTTATTTCAAATACTTCGCTAGATTGTTTCGGACAAGAAACTAGTCAAACGTTTGCAAATGGAAGTGCTCAGGCGGGAAACAGAGGTACCCACGGTGTCAAGGGAAGAAGATTTAAGAATTCAGAAGGTTTTAAACCAATTGGAATTACCTCATCCTTATGTAAAGAAACTTATCTTCTGCATCAGTGTGCTACCTGCAACATTTTATGCTGA

Protein Analysis

373

Amino Acids

41.87

Weight (kDa)

8.5

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 1 - 82 1.7e-19 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0022342)

Species Orthologous Gene IDs
malus_domestica MD15G1066500.v1.1
pyrus_communis pycom15g06280 pycom15g06290
rosa_roxburghii Rroxscaffold_3G00234200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1109
Acc65I GGTACC 1 cut(s) 992
AccB1I GGYRCC 1 cut(s) 992
AciI CCGC 2 cut(s) 428, 980
AclI AACGTT 2 cut(s) 15, 956
AcsI RAATTY 2 cut(s) 363, 1024
AfaI GTAC 3 cut(s) 172, 837, 994
AgsI TTSAA 2 cut(s) 743, 915
AhlI ACTAGT 1 cut(s) 947
AjiI CACGTC 1 cut(s) 632
AleI CACNNNNGTG 1 cut(s) 518
AluBI AGCT 8 cut(s) 28, 115, 289, 317, 322, 334, 689, 752
AluI AGCT 8 cut(s) 28, 115, 289, 317, 322, 334, 689, 752
Alw21I GWGCWC 3 cut(s) 341, 691, 976
Alw26I GTCTC 1 cut(s) 99
Alw44I GTGCAC 1 cut(s) 337
ApaLI GTGCAC 1 cut(s) 337
ApeKI GCWGC 1 cut(s) 286
ApoI RAATTY 2 cut(s) 363, 1024
AseI ATTAAT 1 cut(s) 900
Asp700I GAANNNNTTC 1 cut(s) 363
Asp718I GGTACC 1 cut(s) 992
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 1 cut(s) 582
AsuHPI GGTGA 2 cut(s) 736, 833
AsuII TTCGAA 1 cut(s) 54
AvaII GGWCC 1 cut(s) 582
BaeGI GKGCMC 1 cut(s) 341
BaeI ACNNNNGTAYC 4 cut(s) 258, 291, 976, 1009
BanI GGYRCC 1 cut(s) 992
BanII GRGCYC 1 cut(s) 691
Bbv12I GWGCWC 3 cut(s) 341, 691, 976
BbvI GCAGC 1 cut(s) 298
BccI CCATC 2 cut(s) 170, 367
BcoDI GTCTC 1 cut(s) 99
BcuI ACTAGT 1 cut(s) 947
BfaI CTAG 4 cut(s) 290, 551, 926, 948
BfuAI ACCTGC 1 cut(s) 1109
BisI GCNGC 1 cut(s) 287
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 1 cut(s) 288
Bme18I GGWCC 1 cut(s) 582
BmgBI CACGTC 1 cut(s) 632
BmgT120I GGNCC 1 cut(s) 582
BmiI GGNNCC 2 cut(s) 678, 994
BmsI GCATC 2 cut(s) 244, 1096
Bpu10I CCTNAGC 1 cut(s) 975
Bpu1102I GCTNAGC 1 cut(s) 318
Bpu14I TTCGAA 1 cut(s) 54
BpuEI CTTGAG 3 cut(s) 196, 408, 470
BsaBI GATNNNNATC 1 cut(s) 65
BsaJI CCNNGG 3 cut(s) 139, 543, 997
BsaXI ACNNNNNCTCC 2 cut(s) 491, 521
Bse8I GATNNNNATC 1 cut(s) 65
BseDI CCNNGG 3 cut(s) 139, 543, 997
BseGI GGATG 2 cut(s) 436, 1058
BseJI GATNNNNATC 1 cut(s) 65
BseMII CTCAG 4 cut(s) 43, 179, 897, 989
BseRI GAGGAG 1 cut(s) 603
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 1 cut(s) 298
BshNI GGYRCC 1 cut(s) 992
BsiHKAI GWGCWC 3 cut(s) 341, 691, 976
BsmAI GTCTC 1 cut(s) 99
BsmI GAATGC 1 cut(s) 738
Bsp119I TTCGAA 1 cut(s) 54
Bsp1286I GDGCHC 3 cut(s) 341, 691, 976
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 2 cut(s) 428, 980
BspCNI CTCAG 4 cut(s) 42, 180, 896, 988
BspLI GGNNCC 2 cut(s) 678, 994
BspMI ACCTGC 1 cut(s) 1109
BspT104I TTCGAA 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 992
BssECI CCNNGG 3 cut(s) 139, 543, 997
BssT1I CCWWGG 2 cut(s) 139, 543
Bst4CI ACNGT 2 cut(s) 271, 1001
Bst6I CTCTTC 2 cut(s) 56, 224
BstAPI GCANNNNNTGC 1 cut(s) 575
BstBI TTCGAA 1 cut(s) 54
BstC8I GCNNGC 2 cut(s) 154, 259
BstDEI CTNAG 5 cut(s) 29, 188, 318, 883, 975
BstDSI CCRYGG 1 cut(s) 997
BstF5I GGATG 2 cut(s) 436, 1058
BstHHI GCGC 1 cut(s) 441
BstMAI GTCTC 1 cut(s) 99
BstMWI GCNNNNNNNGC 3 cut(s) 158, 233, 575
BstSLI GKGCMC 1 cut(s) 341
BstV1I GCAGC 1 cut(s) 298
BtgI CCRYGG 1 cut(s) 997
BtgZI GCGATG 1 cut(s) 470
BtrI CACGTC 1 cut(s) 632
BtsCI GGATG 2 cut(s) 436, 1058
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 5 cut(s) 76, 156, 185, 513, 1097
BveI ACCTGC 1 cut(s) 1109
Cac8I GCNNGC 2 cut(s) 154, 259
CfoI GCGC 1 cut(s) 441
Cfr13I GGNCC 1 cut(s) 582
Csp6I GTAC 3 cut(s) 171, 836, 993
CviAII CATG 3 cut(s) 622, 647, 832
CviQI GTAC 3 cut(s) 171, 836, 993
DdeI CTNAG 5 cut(s) 29, 188, 318, 883, 975
DraI TTTAAA 1 cut(s) 1039
Eam1104I CTCTTC 2 cut(s) 56, 224
EarI CTCTTC 2 cut(s) 56, 224
Ecl136II GAGCTC 1 cut(s) 689
Eco130I CCWWGG 2 cut(s) 139, 543
Eco24I GRGCYC 1 cut(s) 691
Eco47I GGWCC 1 cut(s) 582
Eco53kI GAGCTC 1 cut(s) 689
EcoICRI GAGCTC 1 cut(s) 689
EcoRI GAATTC 2 cut(s) 363, 1024
EcoT14I CCWWGG 2 cut(s) 139, 543
EcoT38I GRGCYC 1 cut(s) 691
ErhI CCWWGG 2 cut(s) 139, 543
FaeI CATG 3 cut(s) 625, 650, 835
FatI CATG 3 cut(s) 621, 646, 831
FauI CCCGC 1 cut(s) 973
FauNDI CATATG 1 cut(s) 167
Fnu4HI GCNGC 1 cut(s) 287
FokI GGATG 2 cut(s) 443, 1045
FriOI GRGCYC 1 cut(s) 691
Fsp4HI GCNGC 1 cut(s) 287
FspBI CTAG 4 cut(s) 290, 551, 926, 948
GlaI GCGC 1 cut(s) 440
GluI GCNGC 1 cut(s) 287
HhaI GCGC 1 cut(s) 441
Hin1II CATG 3 cut(s) 625, 650, 835
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HinfI GANTC 4 cut(s) 107, 178, 727, 824
HphI GGTGA 2 cut(s) 736, 833
Hpy166II GTNNAC 4 cut(s) 265, 339, 496, 609
Hpy188I TCNGA 8 cut(s) 23, 280, 459, 661, 673, 886, 938, 1030
Hpy188III TCNNGA 2 cut(s) 207, 743
Hpy8I GTNNAC 4 cut(s) 265, 339, 496, 609
HpyAV CCTTC 1 cut(s) 1025
HpyCH4III ACNGT 2 cut(s) 271, 1001
HpyCH4IV ACGT 4 cut(s) 15, 631, 856, 956
HpyCH4V TGCA 9 cut(s) 130, 304, 339, 578, 738, 870, 962, 1087, 1104
HpyF10VI GCNNNNNNNGC 3 cut(s) 158, 233, 575
HpyF3I CTNAG 5 cut(s) 29, 188, 318, 883, 975
HpySE526I ACGT 4 cut(s) 15, 631, 856, 956
Hsp92II CATG 3 cut(s) 625, 650, 835
HspAI GCGC 1 cut(s) 439
KpnI GGTACC 1 cut(s) 996
LpnPI CCDG 9 cut(s) 60, 195, 218, 271, 308, 742, 797, 962, 1114
Lsp1109I GCAGC 1 cut(s) 298
LweI GCATC 2 cut(s) 244, 1096
MaeI CTAG 4 cut(s) 290, 551, 926, 948
MaeII ACGT 4 cut(s) 15, 631, 856, 956
MaeIII GTNAC 2 cut(s) 244, 560
MboII GAAGA 5 cut(s) 43, 211, 1023, 1026, 1073
MfeI CAATTG 1 cut(s) 1044
MhlI GDGCHC 3 cut(s) 341, 691, 976
MluCI AATT 9 cut(s) 35, 56, 80, 363, 476, 719, 1024, 1044, 1050
MlyI GAGTC 2 cut(s) 101, 187
MroXI GAANNNNTTC 1 cut(s) 363
MseI TTAA 4 cut(s) 83, 900, 1020, 1038
MslI CAYNNNNRTG 3 cut(s) 518, 1063, 1092
MunI CAATTG 1 cut(s) 1044
Mva1269I GAATGC 1 cut(s) 738
MwoI GCNNNNNNNGC 3 cut(s) 158, 233, 575
NdeI CATATG 1 cut(s) 167
NlaIII CATG 3 cut(s) 625, 650, 835
NlaIV GGNNCC 2 cut(s) 678, 994
NmuCI GTSAC 1 cut(s) 560
NspV TTCGAA 1 cut(s) 54
OliI CACNNNNGTG 1 cut(s) 518
PctI GAATGC 1 cut(s) 738
PdmI GAANNNNTTC 1 cut(s) 363
PfeI GAWTC 2 cut(s) 727, 824
PkrI GCNGC 1 cut(s) 288
PleI GAGTC 2 cut(s) 101, 186
PpsI GAGTC 2 cut(s) 101, 186
PshBI ATTAAT 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 691
Psp1406I AACGTT 2 cut(s) 15, 956
PspN4I GGNNCC 2 cut(s) 678, 994
PspPI GGNCC 1 cut(s) 582
RsaI GTAC 3 cut(s) 172, 837, 994
RsaNI GTAC 3 cut(s) 171, 836, 993
RseI CAYNNNNRTG 3 cut(s) 518, 1063, 1092
SacI GAGCTC 1 cut(s) 691
SaqAI TTAA 4 cut(s) 83, 900, 1020, 1038
SatI GCNGC 1 cut(s) 287
Sau96I GGNCC 1 cut(s) 582
SchI GAGTC 2 cut(s) 101, 187
SduI GDGCHC 3 cut(s) 341, 691, 976
SfaNI GCATC 2 cut(s) 244, 1096
SfuI TTCGAA 1 cut(s) 54
SinI GGWCC 1 cut(s) 582
SmiMI CAYNNNNRTG 3 cut(s) 518, 1063, 1092
SmlI CTYRAG 3 cut(s) 175, 423, 449
SmoI CTYRAG 3 cut(s) 175, 423, 449
SpeI ACTAGT 1 cut(s) 947
Sse9I AATT 9 cut(s) 35, 56, 80, 363, 476, 719, 1024, 1044, 1050
SsiI CCGC 2 cut(s) 428, 980
SspMI CTAG 4 cut(s) 290, 551, 926, 948
SstI GAGCTC 1 cut(s) 691
StyI CCWWGG 2 cut(s) 139, 543
TaaI ACNGT 2 cut(s) 271, 1001
TaiI ACGT 4 cut(s) 18, 634, 859, 959
TaqI TCGA 3 cut(s) 54, 208, 685
TasI AATT 9 cut(s) 35, 56, 80, 363, 476, 719, 1024, 1044, 1050
TfiI GAWTC 2 cut(s) 727, 824
Tru1I TTAA 4 cut(s) 83, 900, 1020, 1038
Tru9I TTAA 4 cut(s) 83, 900, 1020, 1038
TscAI CASTG 5 cut(s) 76, 163, 192, 520, 1097
TseFI GTSAC 1 cut(s) 560
TseI GCWGC 1 cut(s) 286
Tsp45I GTSAC 1 cut(s) 560
TspDTI ATGAA 2 cut(s) 610, 663
TspRI CASTG 5 cut(s) 76, 163, 192, 520, 1097
VneI GTGCAC 1 cut(s) 337
VpaK11BI GGWCC 1 cut(s) 582
VspI ATTAAT 1 cut(s) 900
XapI RAATTY 2 cut(s) 363, 1024
XmnI GAANNNNTTC 1 cut(s) 363
XspI CTAG 4 cut(s) 290, 551, 926, 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.