pycom15g10690

3-ketoacyl-CoA synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0022412
Physical Location & Seq
Forward (+)
7157666 .. 7158244
579 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 579 bp
ATGTCACCTTCCAATCTAAACACACCTTTAATCCCTTCATCATCGGTGTCCTCGGCCTCATTGAAGCTTCCCGATTTCAAACAGTCCGTGAAATTGAAGTATGTAAAGCTCGGATACCACTACCTTATCACCCATGGAATGTACCTGTTTTTATCACCTCTTTTTGTTCTCACCTTGGCACAGCTGTCTACATTCTCACTCCAAGACCTCCATGACCTTTGGGATCACCTCAGGTTCAATCTCATATCCGTTGTCCTTTGCTCGACTCTTTTGGTCTTCTTATTGACCCTCTATTTCTTTTCTCGTCCATGGCCTGTTTACCTTGTTGATTTCTCGTGCTATAAGCCGGAGGATGCGCGCAAAGTCACGAGGCAGGTATTCATGAACTGTTCCCATTCGATAGGAACATTCACTGAGGAAAACCTCGCGTTTCAACGCAAAATTCTTGAGAGGTCGGGTCTTGGGGAGTCAACCTACCTCCCTGAAGCTGTTATACGGGTGCCTCCCAATCCATGTATGGCAGAGGCGAGAAAAGAAGCTGAGATGGTAATGTTTGGTGCCCTTGATCGAGCTATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009628 GO:0050896
KEGG Pathways
Metabolic & Signaling
ko00062 ko01110 map00062 map01110
Pfam Domains
Protein Families
ACP_syn_III_C FAE1_CUT1_RppA

Protein Analysis

193

Amino Acids

21.89

Weight (kDa)

8.33

Isoelectric Point (pI)

45.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAE1_CUT1_RppA PF08392 99 - 190 1.6e-29 FAE1/Type III polyketide synthase-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0022412)

Species Orthologous Gene IDs
malus_domestica MD00G1026700.v1.1
pyrus_communis pycom02g03160 pycom15g10690
rosa_multiflora Rmu_co8061556.1_g000001

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 364
AccB1I GGYRCC 2 cut(s) 499, 557
AccI GTMKAC 1 cut(s) 188
AccII CGCG 2 cut(s) 358, 428
AclWI GGATC 1 cut(s) 231
AcsI RAATTY 1 cut(s) 441
AcuI CTGAAG 1 cut(s) 504
AfaI GTAC 1 cut(s) 143
AgsI TTSAA 5 cut(s) 64, 79, 97, 238, 434
AluBI AGCT 6 cut(s) 67, 109, 184, 488, 539, 572
AluI AGCT 6 cut(s) 67, 109, 184, 488, 539, 572
AlwI GGATC 1 cut(s) 231
AoxI GGCC 2 cut(s) 54, 311
ApoI RAATTY 1 cut(s) 441
AspLEI GCGC 2 cut(s) 358, 360
AsuHPI GGTGA 4 cut(s) 121, 147, 163, 218
AxyI CCTNAGG 1 cut(s) 230
BaeGI GKGCMC 1 cut(s) 562
BanI GGYRCC 2 cut(s) 499, 557
BarI GAAGNNNNNNTAC 2 cut(s) 477, 509
BauI CACGAG 2 cut(s) 334, 367
BbsI GAAGAC 1 cut(s) 268
BccI CCATC 1 cut(s) 538
BciVI GTATCC 1 cut(s) 107
BfuAI ACCTGC 1 cut(s) 364
BfuI GTATCC 1 cut(s) 107
BmiI GGNNCC 2 cut(s) 501, 559
BmsI GCATC 1 cut(s) 343
BpiI GAAGAC 1 cut(s) 268
BpuEI CTTGAG 1 cut(s) 467
BsaJI CCNNGG 4 cut(s) 51, 133, 174, 308
Bse21I CCTNAGG 1 cut(s) 230
BseDI CCNNGG 4 cut(s) 51, 133, 174, 308
BseGI GGATG 1 cut(s) 358
BseMII CTCAG 3 cut(s) 244, 405, 531
BsePI GCGCGC 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 562
Bsh1236I CGCG 2 cut(s) 358, 428
BshFI GGCC 2 cut(s) 56, 313
BshNI GGYRCC 2 cut(s) 499, 557
BsiSI CCGG 1 cut(s) 347
BsnI GGCC 2 cut(s) 56, 313
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 2 cut(s) 223, 565
Bsp19I CCATGG 2 cut(s) 133, 308
BspANI GGCC 2 cut(s) 56, 313
BspCNI CTCAG 3 cut(s) 243, 406, 532
BspFNI CGCG 2 cut(s) 358, 428
BspHI TCATGA 1 cut(s) 381
BspLI GGNNCC 2 cut(s) 501, 559
BspMI ACCTGC 1 cut(s) 364
BspPI GGATC 1 cut(s) 231
BspT107I GGYRCC 2 cut(s) 499, 557
BssECI CCNNGG 4 cut(s) 51, 133, 174, 308
BssHII GCGCGC 1 cut(s) 356
BssMI GATC 2 cut(s) 223, 565
BssSI CACGAG 2 cut(s) 334, 367
BssT1I CCWWGG 3 cut(s) 133, 174, 308
Bst2BI CACGAG 2 cut(s) 334, 367
Bst4CI ACNGT 2 cut(s) 84, 389
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 3 cut(s) 230, 414, 540
BstDSI CCRYGG 2 cut(s) 133, 308
BstF5I GGATG 1 cut(s) 358
BstFNI CGCG 2 cut(s) 358, 428
BstHHI GCGC 2 cut(s) 358, 360
BstKTI GATC 2 cut(s) 226, 568
BstMBI GATC 2 cut(s) 223, 565
BstSLI GKGCMC 1 cut(s) 562
BstUI CGCG 2 cut(s) 358, 428
BstV2I GAAGAC 1 cut(s) 268
Bsu36I CCTNAGG 1 cut(s) 230
BsuI GTATCC 1 cut(s) 107
BsuRI GGCC 2 cut(s) 56, 313
BtgI CCRYGG 2 cut(s) 133, 308
BtsCI GGATG 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 358
CciI TCATGA 1 cut(s) 381
CfoI GCGC 2 cut(s) 358, 360
Csp6I GTAC 1 cut(s) 142
CviAII CATG 5 cut(s) 134, 212, 309, 382, 513
CviJI RGCY 9 cut(s) 56, 67, 109, 184, 313, 346, 488, 539, 572
CviKI_1 RGCY 9 cut(s) 56, 67, 109, 184, 313, 346, 488, 539, 572
CviQI GTAC 1 cut(s) 142
DdeI CTNAG 3 cut(s) 230, 414, 540
DpnI GATC 2 cut(s) 225, 567
DpnII GATC 2 cut(s) 223, 565
Eco130I CCWWGG 3 cut(s) 133, 174, 308
Eco57I CTGAAG 1 cut(s) 504
Eco81I CCTNAGG 1 cut(s) 230
EcoT14I CCWWGG 3 cut(s) 133, 174, 308
ErhI CCWWGG 3 cut(s) 133, 174, 308
FaeI CATG 5 cut(s) 137, 215, 312, 385, 516
FatI CATG 5 cut(s) 133, 211, 308, 381, 512
FblI GTMKAC 1 cut(s) 188
FokI GGATG 1 cut(s) 365
GlaI GCGC 2 cut(s) 357, 359
HaeIII GGCC 2 cut(s) 56, 313
HapII CCGG 1 cut(s) 347
HhaI GCGC 2 cut(s) 358, 360
Hin1II CATG 5 cut(s) 137, 215, 312, 385, 516
Hin6I GCGC 2 cut(s) 356, 358
HinP1I GCGC 2 cut(s) 356, 358
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
HindIII AAGCTT 1 cut(s) 65
HinfI GANTC 2 cut(s) 265, 467
HpaII CCGG 1 cut(s) 347
HphI GGTGA 4 cut(s) 121, 147, 163, 218
Hpy166II GTNNAC 3 cut(s) 189, 319, 471
Hpy188I TCNGA 1 cut(s) 113
Hpy188III TCNNGA 4 cut(s) 71, 367, 382, 446
Hpy8I GTNNAC 3 cut(s) 189, 319, 471
HpyAV CCTTC 2 cut(s) 18, 45
HpyCH4III ACNGT 2 cut(s) 84, 389
HpyF3I CTNAG 3 cut(s) 230, 414, 540
Hsp92II CATG 5 cut(s) 137, 215, 312, 385, 516
HspAI GCGC 2 cut(s) 356, 358
Kzo9I GATC 2 cut(s) 223, 565
LpnPI CCDG 6 cut(s) 158, 217, 327, 359, 360, 495
LweI GCATC 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 3, 364
MalI GATC 2 cut(s) 225, 567
MboI GATC 2 cut(s) 223, 565
MboII GAAGA 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 2 cut(s) 92, 441
MlyI GAGTC 2 cut(s) 259, 476
MseI TTAA 1 cut(s) 29
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 1 cut(s) 347
MvnI CGCG 2 cut(s) 358, 428
NcoI CCATGG 2 cut(s) 133, 308
NdeII GATC 2 cut(s) 223, 565
NlaIII CATG 5 cut(s) 137, 215, 312, 385, 516
NlaIV GGNNCC 2 cut(s) 501, 559
NmeAIII GCCGAG 1 cut(s) 32
NmuCI GTSAC 2 cut(s) 3, 364
PagI TCATGA 1 cut(s) 381
PauI GCGCGC 1 cut(s) 356
PleI GAGTC 2 cut(s) 259, 475
PpsI GAGTC 2 cut(s) 259, 475
PspN4I GGNNCC 2 cut(s) 501, 559
PteI GCGCGC 1 cut(s) 356
PvuII CAGCTG 1 cut(s) 184
RsaI GTAC 1 cut(s) 143
RsaNI GTAC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 29
Sau3AI GATC 2 cut(s) 223, 565
SchI GAGTC 2 cut(s) 259, 476
SduI GDGCHC 1 cut(s) 562
SfaNI GCATC 1 cut(s) 343
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 2 cut(s) 92, 441
StyI CCWWGG 3 cut(s) 133, 174, 308
TaaI ACNGT 2 cut(s) 84, 389
TaqI TCGA 3 cut(s) 263, 398, 568
TasI AATT 2 cut(s) 92, 441
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 1 cut(s) 418
TseFI GTSAC 2 cut(s) 3, 364
Tsp45I GTSAC 2 cut(s) 3, 364
TspDTI ATGAA 3 cut(s) 27, 370, 398
TspGWI ACGGA 2 cut(s) 76, 238
TspRI CASTG 1 cut(s) 418
XapI RAATTY 1 cut(s) 441
XcmI CCANNNNNNNNNTGG 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.