Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0015392

Physical Location & Seq

Genomic Coordinates Forward (+)

8236348 .. 8238213

Gene Structure 1866 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g12090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1866 bp

ATGATGGAAGATCGATCGAGAGGAAGAGCAGGAGGAGTTGGTGGGCAAGAAGTCCTCAAGGACGTCAACTTGCGAGCAAGGCCATGGGAGATCCTCGCCATTGTTGGTCCCAGTGGAGCTGGTAAATCTTCCCTCCTTGAGATTCTCGCCGGCAAACTCACCCCGCACGGCGGTTCCGTTTTCGTCAACCAAAAGCCTCTCCGCCAACCCTACTTCAACAAGGTCTCCGGCTACGTCACCCAAAAGGACACCTTATTTCCTTTGCTTACAGTCGAAGAAACCCTAATGTTCAGCGCCAAGCTCAGGCTCAGGGTTTCTCCCCTTGAGCTCAGCTCCCGCGTCAAGGCCTTAATCAACGAGCTCGGCCTCGCCCAAGTCGCCGCCGCCAGAGTCGGAGACGACAGAGTTCGGGGGATCTCCGGCGGAGAGAGGCGGCGTGTCTCCATCGGAGTCGACGTCATCCACGACCCCGAAGTGCTCATCCTCGACGAGCCCACTTCCGGGCTTGACAGCACCTCCGCCCTTCAGATCATTGACATGCTCAAGACCATGGCTGAGACCAGGGGCAGAACCGTCATTCTGAGCATTCATCAGCCGGGATTCAGAATTGTGAAGCTCTTCAACTCAATCCTTTTGCTGGCCAACGGCTCCGTCCTCCACCACGGCACCGTCGATCATCTCGGCCTCAACTTAAGGCTCATGGGGTTGGAGCTCCCTCTTCATGTCAACGTCGTCGAGTTTGCAATCGACTCCATCGAAACCATTCAACAACAACACCATTCGGCACCACACAATAATATTCTTGTTCTTGATCGTCAAAGTCGAACTGGTAAGCTGACTCTGCAGCAGCTGTTTCAACAATCGAAGACCAATAATAGTAATGCCGGTGGCGGAGGCGGAGGAGGAGTAGTGCCACTTGAAGTTGACGACGATAAAATCACCAGTAATGAAGTTTTTCCCGATTTGGATTTTACTAATTCGAGACTGCGAGAGACGATGATCCTGAGTCATAGGTTCTCGAAGAACATATTCCGAACAAAGGAGCTGTTCGCGTGCCGGACAATTCAGATGCTCGCCTCCGGACTAGTTCTAGGCTCCATCTTTTACGACCTCAAAGACGATCTCACCGGAGCCGAGGAAAGGGTAGGTCTATTCGCATTCATTTTAACGTTTTTGCTGTCGTGCACGACGGAAGCACTGCCGATATTTCTTCAAGAGAGGGAGATTCTGATGAAGGAGACTTGTTGCGGAAGCTACAGGGTCTCGTCTTACGCCATCGCCAACGGATTGGTTTACCTGCCCTTTCTACTAATCCTGGCCGTCCTTTTCACTCTGCCGCTGTACTGGCTGGTCGGACTCAACCCTAACTTCACGGCCTTCTTCCATTTCTTGTTGTTGATATGGTTGATATTATACACTGCGAATTCCGTGGTGGTGTGTTTCAGTGCACTCGTGCCTAATTTCATCGTGGGAAACTCGGTCATTGCCGGCGTCATGGGGTCTTTCTTTCTCTTCTCCGGTTACTTCATCTCCAAGCATGAGATTCCCGGGTACTGGATTTTCATGCACTACATATCGCTGTTCAAGTATCCGTTCGAAGGGTTTCTGATAAACGAGTTCTCAGGGTCGGACAAGTGCTTGGAGTATTTCTTTGGGAGGTGTATGGTGACGGGAGAAGAAGTTCTCAGAGATGAAGGGTATGGGGAGGAGAGCCGGTGGAGGAATGTGGCGGTCATGGTCTGCTTCGTCTTGTTTTACAGGTTTATTTCTTATGTCATTCTTAGATGCAGATGTCGATGCTCCCAGTCCCAAAGGGGCTTCAAACCTACTCCTCCTCCTACCCTCACTGCAACTCCTTTTCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005575 GO:0005623 GO:0005886 GO:0006810 GO:0008150 GO:0015399 GO:0015405 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022804 GO:0022857 GO:0031224 GO:0042623 GO:0042626 GO:0043492 GO:0044425 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko01523 ko02010 ko04976 map01523 map02010 map04976

Pfam Domains

Protein Families

ABC2_membrane ABC_tran

Protein Analysis

622

Amino Acids

69.25

Weight (kDa)

7.96

Isoelectric Point (pI)

41.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ABC_tran	PF00005	19 - 167	7.7e-31	ABC transporter
ABC2_membrane	PF01061	332 - 540	4e-50	ABC-2 type transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015392)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13610
fragaria_vesca	FvH4_1g24520
malus_domestica	MD15G1134300.v1.1
prunus_persica	Prupe.4G272400_v2.0.a1
pyrus_communis	pycom15g12090
rosa_chinensis	RchiOBHm_Chr2g0121921
rosa_laevigata	RLG00000018633
rosa_multiflora	Rmu_co8491703.1_g000001 Rmu_sc0003880.1_g000031
rosa_roxburghii	Rroxscaffold_2G00122210
rosa_rugosa	Rorug02G0234800
rosa_samantha	Rh2AG291600 Rh2BG302100 Rh2CG280900 Rh2DG316100
rosa_wichuraiana	Rw2G023400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	66, 459
Acc36I	ACCTGC	1 cut(s)	1305
AccB1I	GGYRCC	2 cut(s)	665, 784
AccI	GTMKAC	1 cut(s)	453
AccII	CGCG	2 cut(s)	339, 1052
AccIII	TCCGGA	1 cut(s)	1079
AclI	AACGTT	1 cut(s)	1169
AclWI	GGATC	3 cut(s)	85, 422, 994
AcoI	YGGCCR	2 cut(s)	639, 1317
AcsI	RAATTY	1 cut(s)	1423
AcuI	CTGAAG	1 cut(s)	509
AcyI	GRCGYC	3 cut(s)	63, 456, 1491
AfaI	GTAC	2 cut(s)	1343, 1553
AflII	CTTAAG	1 cut(s)	691
AgsI	TTSAA	8 cut(s)	217, 622, 767, 857, 920, 1214, 1585, 1822
AhlI	ACTAGT	1 cut(s)	1084
AjnI	CCWGG	2 cut(s)	560, 1314
AloI	GAACNNNNNNTCC	2 cut(s)	1665, 1697
Alw21I	GWGCWC	6 cut(s)	330, 363, 480, 714, 1187, 1450
Alw26I	GTCTC	8 cut(s)	229, 390, 445, 551, 976, 986, 1232, 1267
Alw44I	GTGCAC	2 cut(s)	1183, 1446
AlwI	GGATC	3 cut(s)	85, 422, 994
AlwNI	CAGNNNCTG	1 cut(s)	850
Ama87I	CYCGRG	1 cut(s)	1547
Aor13HI	TCCGGA	1 cut(s)	1079
AoxI	GGCC	7 cut(s)	80, 345, 364, 639, 682, 1317, 1374
ApaLI	GTGCAC	2 cut(s)	1183, 1446
ApeKI	GCWGC	2 cut(s)	844, 847
ApoI	RAATTY	1 cut(s)	1423
Asp700I	GAANNNNTTC	6 cut(s)	617, 762, 954, 1028, 1602, 1680
AspLEI	GCGC	1 cut(s)	296
AspS9I	GGNCC	1 cut(s)	107
AsuC2I	CCSGG	4 cut(s)	502, 597, 1548, 1549
AsuHPI	GGTGA	5 cut(s)	151, 229, 931, 1117, 1678
AsuII	TTCGAA	1 cut(s)	1596
AvaI	CYCGRG	1 cut(s)	1547
AvaII	GGWCC	1 cut(s)	107
BaeGI	GKGCMC	2 cut(s)	1187, 1450
BalI	TGGCCA	1 cut(s)	641
BanI	GGYRCC	2 cut(s)	665, 784
BanII	GRGCYC	4 cut(s)	330, 363, 495, 714
BauI	CACGAG	1 cut(s)	1451
BbsI	GAAGAC	1 cut(s)	872
Bbv12I	GWGCWC	6 cut(s)	330, 363, 480, 714, 1187, 1450
BbvI	GCAGC	2 cut(s)	856, 859
BccI	CCATC	4 cut(s)	452, 761, 1106, 1283
BceAI	ACGGC	5 cut(s)	184, 661, 679, 1304, 1389
BciT130I	CCWGG	2 cut(s)	562, 1316
BciVI	GTATCC	1 cut(s)	1599
BcnI	CCSGG	4 cut(s)	502, 597, 1548, 1549
BcoDI	GTCTC	8 cut(s)	229, 390, 445, 551, 976, 986, 1232, 1267
BcuI	ACTAGT	1 cut(s)	1084
BfaI	CTAG	2 cut(s)	1085, 1091
BfmI	CTRYAG	2 cut(s)	842, 1255
BfoI	RGCGCY	1 cut(s)	297
BfrI	CTTAAG	1 cut(s)	691
BfuAI	ACCTGC	1 cut(s)	1305
BfuI	GTATCC	1 cut(s)	1599
BisI	GCNGC	6 cut(s)	381, 384, 434, 845, 848, 1337
BlpI	GCTNAGC	1 cut(s)	329
BlsI	GCNGC	6 cut(s)	382, 385, 435, 846, 849, 1338
Bme1390I	CCNGG	6 cut(s)	502, 562, 597, 1316, 1548, 1549
Bme18I	GGWCC	1 cut(s)	107
BmeT110I	CYCGRG	1 cut(s)	1547
BmgT120I	GGNCC	1 cut(s)	107
BmiI	GGNNCC	7 cut(s)	109, 175, 649, 667, 786, 1096, 1132
BmrFI	CCNGG	6 cut(s)	502, 562, 597, 1316, 1548, 1549
BmrI	ACTGGG	2 cut(s)	105, 1798
BmsI	GCATC	3 cut(s)	1059, 1775, 1787
BmuI	ACTGGG	2 cut(s)	105, 1798
BpiI	GAAGAC	1 cut(s)	872
BplI	GAGNNNNNCTC	2 cut(s)	317, 349
Bpu10I	CCTNAGC	2 cut(s)	302, 308
Bpu1102I	GCTNAGC	1 cut(s)	329
Bpu14I	TTCGAA	1 cut(s)	1596
BpuEI	CTTGAG	4 cut(s)	41, 158, 344, 527
BpuMI	CCSGG	4 cut(s)	502, 597, 1548, 1549
Bsa29I	ATCGAT	1 cut(s)	13
BsaHI	GRCGYC	3 cut(s)	63, 456, 1491
BsaI	GGTCTC	3 cut(s)	229, 551, 1267
BsaJI	CCNNGG	7 cut(s)	83, 549, 561, 661, 1134, 1428, 1547
BsaWI	WCCGGW	3 cut(s)	1079, 1127, 1517
Bse118I	RCCGGY	4 cut(s)	149, 884, 1487, 1713
Bse1I	ACTGG	6 cut(s)	111, 832, 942, 1349, 1559, 1804
Bse3DI	GCAATG	1 cut(s)	1482
BseAI	TCCGGA	1 cut(s)	1079
BseBI	CCWGG	2 cut(s)	562, 1316
BseCI	ATCGAT	1 cut(s)	13
BseDI	CCNNGG	7 cut(s)	83, 549, 561, 661, 1134, 1428, 1547
BseGI	GGATG	2 cut(s)	459, 480
BseMI	GCAATG	1 cut(s)	1482
BseMII	CTCAG	8 cut(s)	316, 322, 343, 546, 572, 995, 1635, 1699
BseNI	ACTGG	6 cut(s)	111, 832, 942, 1349, 1559, 1804
BseRI	GAGGAG	6 cut(s)	48, 915, 918, 1721, 1821, 1824
BseSI	GKGCMC	2 cut(s)	1187, 1450
BseXI	GCAGC	2 cut(s)	856, 859
Bsh1236I	CGCG	2 cut(s)	339, 1052
Bsh1285I	CGRYCG	1 cut(s)	17
BshFI	GGCC	7 cut(s)	82, 347, 366, 641, 684, 1319, 1376
BshNI	GGYRCC	2 cut(s)	665, 784
BshVI	ATCGAT	1 cut(s)	13
BsiEI	CGRYCG	1 cut(s)	17
BsiHKAI	GWGCWC	6 cut(s)	330, 363, 480, 714, 1187, 1450
BsiHKCI	CYCGRG	1 cut(s)	1547
BslFI	GGGAC	2 cut(s)	93, 1792
BsmAI	GTCTC	8 cut(s)	229, 390, 445, 551, 976, 986, 1232, 1267
BsmBI	CGTCTC	2 cut(s)	390, 986
BsmFI	GGGAC	2 cut(s)	93, 1792
BsmI	GAATGC	2 cut(s)	585, 1157
BsnI	GGCC	7 cut(s)	82, 347, 366, 641, 684, 1319, 1376
Bso31I	GGTCTC	3 cut(s)	229, 551, 1267
BsoBI	CYCGRG	1 cut(s)	1547
Bsp119I	TTCGAA	1 cut(s)	1596
Bsp1286I	GDGCHC	7 cut(s)	330, 363, 480, 495, 714, 1187, 1450
Bsp13I	TCCGGA	1 cut(s)	1079
Bsp143I	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
Bsp1720I	GCTNAGC	1 cut(s)	329
Bsp19I	CCATGG	2 cut(s)	83, 549
BspANI	GGCC	7 cut(s)	82, 347, 366, 641, 684, 1319, 1376
BspCNI	CTCAG	8 cut(s)	315, 321, 342, 547, 573, 996, 1634, 1698
BspDI	ATCGAT	1 cut(s)	13
BspEI	TCCGGA	1 cut(s)	1079
BspFNI	CGCG	2 cut(s)	339, 1052
BspLI	GGNNCC	7 cut(s)	109, 175, 649, 667, 786, 1096, 1132
BspMAI	CTGCAG	1 cut(s)	846
BspMI	ACCTGC	1 cut(s)	1305
BspPI	GGATC	3 cut(s)	85, 422, 994
BspQI	GCTCTTC	2 cut(s)	19, 623
BspT104I	TTCGAA	1 cut(s)	1596
BspT107I	GGYRCC	2 cut(s)	665, 784
BspTI	CTTAAG	1 cut(s)	691
BspTNI	GGTCTC	3 cut(s)	229, 551, 1267
BsrDI	GCAATG	1 cut(s)	1482
BsrFI	RCCGGY	4 cut(s)	149, 884, 1487, 1713
BsrI	ACTGG	6 cut(s)	111, 832, 942, 1349, 1559, 1804
BssAI	RCCGGY	4 cut(s)	149, 884, 1487, 1713
BssECI	CCNNGG	7 cut(s)	83, 549, 561, 661, 1134, 1428, 1547
BssMI	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
BssNI	GRCGYC	3 cut(s)	63, 456, 1491
BssSI	CACGAG	1 cut(s)	1451
BssT1I	CCWWGG	2 cut(s)	83, 549
Bst2BI	CACGAG	1 cut(s)	1451
Bst2UI	CCWGG	2 cut(s)	562, 1316
Bst4CI	ACNGT	3 cut(s)	271, 574, 670
Bst6I	CTCTTC	4 cut(s)	19, 623, 723, 1517
BstACI	GRCGYC	3 cut(s)	63, 456, 1491
BstAFI	CTTAAG	1 cut(s)	691
BstBI	TTCGAA	1 cut(s)	1596
BstC8I	GCNNGC	6 cut(s)	75, 151, 639, 1054, 1074, 1489
BstDEI	CTNAG	9 cut(s)	302, 308, 329, 555, 581, 1004, 1621, 1685, 1781
BstDSI	CCRYGG	4 cut(s)	83, 549, 661, 1428
BstF5I	GGATG	2 cut(s)	459, 480
BstFNI	CGCG	2 cut(s)	339, 1052
BstH2I	RGCGCY	1 cut(s)	297
BstHHI	GCGC	1 cut(s)	296
BstKTI	GATC	9 cut(s)	13, 17, 93, 417, 531, 676, 814, 1002, 1123
BstMAI	GTCTC	8 cut(s)	229, 390, 445, 551, 976, 986, 1232, 1267
BstMBI	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
BstMCI	CGRYCG	1 cut(s)	17
BstMWI	GCNNNNNNNGC	4 cut(s)	79, 377, 841, 1345
BstNI	CCWGG	2 cut(s)	562, 1316
BstNSI	RCATGY	1 cut(s)	541
BstSCI	CCNGG	6 cut(s)	500, 560, 595, 1314, 1546, 1547
BstSFI	CTRYAG	2 cut(s)	842, 1255
BstSLI	GKGCMC	2 cut(s)	1187, 1450
BstUI	CGCG	2 cut(s)	339, 1052
BstV1I	GCAGC	2 cut(s)	856, 859
BstV2I	GAAGAC	1 cut(s)	872
BstX2I	RGATCY	2 cut(s)	90, 414
BstXI	CCANNNNNNTGG	1 cut(s)	1288
BstYI	RGATCY	2 cut(s)	90, 414
Bsu15I	ATCGAT	1 cut(s)	13
BsuI	GTATCC	1 cut(s)	1599
BsuRI	GGCC	7 cut(s)	82, 347, 366, 641, 684, 1319, 1376
BsuTUI	ATCGAT	1 cut(s)	13
BtgI	CCRYGG	4 cut(s)	83, 549, 661, 1428
BtgZI	GCGATG	1 cut(s)	1261
BtsCI	GGATG	2 cut(s)	459, 480
BtsI	GCAGTG	3 cut(s)	1196, 1416, 1845
BtsIMutI	CAGTG	5 cut(s)	118, 1196, 1416, 1450, 1845
BveI	ACCTGC	1 cut(s)	1305
Cac8I	GCNNGC	6 cut(s)	75, 151, 639, 1054, 1074, 1489
CaiI	CAGNNNCTG	1 cut(s)	850
CfoI	GCGC	1 cut(s)	296
Cfr10I	RCCGGY	4 cut(s)	149, 884, 1487, 1713
Cfr13I	GGNCC	1 cut(s)	107
Cfr9I	CCCGGG	1 cut(s)	1547
ClaI	ATCGAT	1 cut(s)	13
CseI	GACGC	2 cut(s)	328, 1480
Csp6I	GTAC	2 cut(s)	1342, 1552
CviAII	CATG	9 cut(s)	84, 538, 550, 700, 722, 1495, 1538, 1564, 1735
CviQI	GTAC	2 cut(s)	1342, 1552
DdeI	CTNAG	9 cut(s)	302, 308, 329, 555, 581, 1004, 1621, 1685, 1781
DpnI	GATC	9 cut(s)	12, 16, 92, 416, 530, 675, 813, 1001, 1122
DpnII	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
EaeI	YGGCCR	2 cut(s)	639, 1317
Eam1104I	CTCTTC	4 cut(s)	19, 623, 723, 1517
EarI	CTCTTC	4 cut(s)	19, 623, 723, 1517
EciI	GGCGGA	5 cut(s)	191, 438, 508, 906, 912
Ecl136II	GAGCTC	3 cut(s)	328, 361, 712
Eco130I	CCWWGG	2 cut(s)	83, 549
Eco147I	AGGCCT	1 cut(s)	347
Eco24I	GRGCYC	4 cut(s)	330, 363, 495, 714
Eco31I	GGTCTC	3 cut(s)	229, 551, 1267
Eco47I	GGWCC	1 cut(s)	107
Eco53kI	GAGCTC	3 cut(s)	328, 361, 712
Eco57I	CTGAAG	1 cut(s)	509
Eco88I	CYCGRG	1 cut(s)	1547
EcoICRI	GAGCTC	3 cut(s)	328, 361, 712
EcoRI	GAATTC	1 cut(s)	1423
EcoRII	CCWGG	2 cut(s)	560, 1314
EcoT14I	CCWWGG	2 cut(s)	83, 549
EcoT38I	GRGCYC	4 cut(s)	330, 363, 495, 714
ErhI	CCWWGG	2 cut(s)	83, 549
Esp3I	CGTCTC	2 cut(s)	390, 986
FaeI	CATG	9 cut(s)	87, 541, 553, 703, 725, 1498, 1541, 1567, 1738
FalI	AAGNNNNNCTT	2 cut(s)	236, 268
FaqI	GGGAC	2 cut(s)	93, 1792
FatI	CATG	9 cut(s)	83, 537, 549, 699, 721, 1494, 1537, 1563, 1734
FauI	CCCGC	2 cut(s)	171, 344
FblI	GTMKAC	1 cut(s)	453
Fnu4HI	GCNGC	6 cut(s)	381, 384, 434, 845, 848, 1337
FokI	GGATG	2 cut(s)	446, 467
FriOI	GRGCYC	4 cut(s)	330, 363, 495, 714
Fsp4HI	GCNGC	6 cut(s)	381, 384, 434, 845, 848, 1337
FspBI	CTAG	2 cut(s)	1085, 1091
GlaI	GCGC	1 cut(s)	295
GluI	GCNGC	6 cut(s)	381, 384, 434, 845, 848, 1337
HaeII	RGCGCY	1 cut(s)	297
HaeIII	GGCC	7 cut(s)	82, 347, 366, 641, 684, 1319, 1376
HgaI	GACGC	2 cut(s)	328, 1480
HhaI	GCGC	1 cut(s)	296
Hin1I	GRCGYC	3 cut(s)	63, 456, 1491
Hin1II	CATG	9 cut(s)	87, 541, 553, 703, 725, 1498, 1541, 1567, 1738
Hin6I	GCGC	1 cut(s)	294
HinP1I	GCGC	1 cut(s)	294
HincII	GTYRAC	5 cut(s)	67, 187, 454, 727, 925
HindII	GTYRAC	5 cut(s)	67, 187, 454, 727, 925
HphI	GGTGA	5 cut(s)	151, 229, 931, 1117, 1678
Hpy166II	GTNNAC	8 cut(s)	67, 187, 454, 727, 925, 1185, 1294, 1448
Hpy188III	TCNNGA	9 cut(s)	18, 544, 809, 959, 981, 1003, 1018, 1080, 1214
Hpy8I	GTNNAC	8 cut(s)	67, 187, 454, 727, 925, 1185, 1294, 1448
Hpy99I	CGWCG	7 cut(s)	458, 491, 674, 734, 737, 932, 1192
HpyAV	CCTTC	5 cut(s)	533, 1228, 1387, 1592, 1688
HpyCH4III	ACNGT	3 cut(s)	271, 574, 670
HpyCH4IV	ACGT	5 cut(s)	63, 234, 456, 729, 1169
HpyCH4V	TGCA	7 cut(s)	743, 844, 1185, 1448, 1567, 1788, 1850
HpyF10VI	GCNNNNNNNGC	4 cut(s)	79, 377, 841, 1345
HpyF3I	CTNAG	9 cut(s)	302, 308, 329, 555, 581, 1004, 1621, 1685, 1781
HpySE526I	ACGT	5 cut(s)	63, 234, 456, 729, 1169
Hsp92I	GRCGYC	3 cut(s)	63, 456, 1491
Hsp92II	CATG	9 cut(s)	87, 541, 553, 703, 725, 1498, 1541, 1567, 1738
HspAI	GCGC	1 cut(s)	294
Kpn2I	TCCGGA	1 cut(s)	1079
KroI	GCCGGC	2 cut(s)	149, 1487
KroNI	GCCGGC	2 cut(s)	151, 1489
Kzo9I	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
LguI	GCTCTTC	2 cut(s)	19, 623
LmnI	GCTCC	9 cut(s)	116, 338, 653, 709, 717, 1042, 1100, 1130, 1805
Lsp1109I	GCAGC	2 cut(s)	856, 859
LweI	GCATC	3 cut(s)	1059, 1775, 1787
MaeI	CTAG	2 cut(s)	1085, 1091
MaeII	ACGT	5 cut(s)	63, 234, 456, 729, 1169
MaeIII	GTNAC	3 cut(s)	235, 1520, 1666
MalI	GATC	9 cut(s)	12, 16, 92, 416, 530, 675, 813, 1001, 1122
MboI	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
MflI	RGATCY	2 cut(s)	90, 414
MhlI	GDGCHC	7 cut(s)	330, 363, 480, 495, 714, 1187, 1450
MlsI	TGGCCA	1 cut(s)	641
MluCI	AATT	5 cut(s)	606, 976, 1062, 1423, 1459
MluNI	TGGCCA	1 cut(s)	641
MlyI	GAGTC	6 cut(s)	399, 459, 743, 832, 1015, 1350
MmeI	TCCRAC	4 cut(s)	373, 687, 1333, 1608
Mox20I	TGGCCA	1 cut(s)	641
MroI	TCCGGA	1 cut(s)	1079
MroNI	GCCGGC	2 cut(s)	149, 1487
MroXI	GAANNNNTTC	6 cut(s)	617, 762, 954, 1028, 1602, 1680
MscI	TGGCCA	1 cut(s)	641
MseI	TTAA	4 cut(s)	350, 692, 1166, 1864
MslI	CAYNNNNRTG	1 cut(s)	536
Msp20I	TGGCCA	1 cut(s)	641
MspA1I	CMGCKG	2 cut(s)	850, 1339
MspCI	CTTAAG	1 cut(s)	691
MspR9I	CCNGG	6 cut(s)	502, 562, 597, 1316, 1548, 1549
Mva1269I	GAATGC	2 cut(s)	585, 1157
MvaI	CCWGG	2 cut(s)	562, 1316
MvnI	CGCG	2 cut(s)	339, 1052
MwoI	GCNNNNNNNGC	4 cut(s)	79, 377, 841, 1345
NaeI	GCCGGC	2 cut(s)	151, 1489
NciI	CCSGG	4 cut(s)	502, 597, 1548, 1549
NcoI	CCATGG	2 cut(s)	83, 549
NdeII	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
NgoMIV	GCCGGC	2 cut(s)	149, 1487
NlaIII	CATG	9 cut(s)	87, 541, 553, 703, 725, 1498, 1541, 1567, 1738
NlaIV	GGNNCC	7 cut(s)	109, 175, 649, 667, 786, 1096, 1132
NmeAIII	GCCGAG	3 cut(s)	342, 660, 1159
NmuCI	GTSAC	2 cut(s)	235, 1666
NspI	RCATGY	1 cut(s)	541
NspV	TTCGAA	1 cut(s)	1596
PceI	AGGCCT	1 cut(s)	347
PciSI	GCTCTTC	2 cut(s)	19, 623
PcsI	WCGNNNNNNNCGW	6 cut(s)	174, 453, 462, 669, 753, 820
PctI	GAATGC	2 cut(s)	585, 1157
PdiI	GCCGGC	2 cut(s)	151, 1489
PdmI	GAANNNNTTC	6 cut(s)	617, 762, 954, 1028, 1602, 1680
PfeI	GAWTC	4 cut(s)	142, 600, 1225, 1543
PkrI	GCNGC	6 cut(s)	382, 385, 435, 846, 849, 1338
Ple19I	CGATCG	1 cut(s)	17
PleI	GAGTC	6 cut(s)	398, 458, 743, 832, 1014, 1350
PpsI	GAGTC	6 cut(s)	398, 458, 743, 832, 1014, 1350
Psp124BI	GAGCTC	3 cut(s)	330, 363, 714
Psp1406I	AACGTT	1 cut(s)	1169
Psp6I	CCWGG	2 cut(s)	560, 1314
PspGI	CCWGG	2 cut(s)	560, 1314
PspN4I	GGNNCC	7 cut(s)	109, 175, 649, 667, 786, 1096, 1132
PspPI	GGNCC	1 cut(s)	107
PstI	CTGCAG	1 cut(s)	846
PstNI	CAGNNNCTG	1 cut(s)	850
PsuI	RGATCY	2 cut(s)	90, 414
PvuI	CGATCG	1 cut(s)	17
PvuII	CAGCTG	1 cut(s)	850
RsaI	GTAC	2 cut(s)	1343, 1553
RsaNI	GTAC	2 cut(s)	1342, 1552
RseI	CAYNNNNRTG	1 cut(s)	536
SacI	GAGCTC	3 cut(s)	330, 363, 714
SalI	GTCGAC	1 cut(s)	452
SapI	GCTCTTC	2 cut(s)	19, 623
SaqAI	TTAA	4 cut(s)	350, 692, 1166, 1864
SatI	GCNGC	6 cut(s)	381, 384, 434, 845, 848, 1337
Sau3AI	GATC	9 cut(s)	10, 14, 90, 414, 528, 673, 811, 999, 1120
Sau96I	GGNCC	1 cut(s)	107
SchI	GAGTC	6 cut(s)	399, 459, 743, 832, 1015, 1350
ScrFI	CCNGG	6 cut(s)	502, 562, 597, 1316, 1548, 1549
SduI	GDGCHC	7 cut(s)	330, 363, 480, 495, 714, 1187, 1450
SfaNI	GCATC	3 cut(s)	1059, 1775, 1787
SfcI	CTRYAG	2 cut(s)	842, 1255
SfuI	TTCGAA	1 cut(s)	1596
SinI	GGWCC	1 cut(s)	107
SmaI	CCCGGG	1 cut(s)	1549
SmiMI	CAYNNNNRTG	1 cut(s)	536
SmlI	CTYRAG	5 cut(s)	56, 137, 323, 542, 691
SmoI	CTYRAG	5 cut(s)	56, 137, 323, 542, 691
SpeI	ACTAGT	1 cut(s)	1084
Sse9I	AATT	5 cut(s)	606, 976, 1062, 1423, 1459
SseBI	AGGCCT	1 cut(s)	347
SspI	AATATT	1 cut(s)	799
SspMI	CTAG	2 cut(s)	1085, 1091
SstI	GAGCTC	3 cut(s)	330, 363, 714
StuI	AGGCCT	1 cut(s)	347
StyD4I	CCNGG	6 cut(s)	500, 560, 595, 1314, 1546, 1547
StyI	CCWWGG	2 cut(s)	83, 549
TaaI	ACNGT	3 cut(s)	271, 574, 670
TaiI	ACGT	5 cut(s)	66, 237, 459, 732, 1172
TaqII	GACCGA	1 cut(s)	1468
TasI	AATT	5 cut(s)	606, 976, 1062, 1423, 1459
TatI	WGTACW	1 cut(s)	1341
TauI	GCSGC	4 cut(s)	383, 386, 436, 1339
TfiI	GAWTC	4 cut(s)	142, 600, 1225, 1543
Tru1I	TTAA	4 cut(s)	350, 692, 1166, 1864
Tru9I	TTAA	4 cut(s)	350, 692, 1166, 1864
TscAI	CASTG	5 cut(s)	118, 1203, 1423, 1450, 1852
TseFI	GTSAC	2 cut(s)	235, 1666
TseI	GCWGC	2 cut(s)	844, 847
Tsp45I	GTSAC	2 cut(s)	235, 1666
TspDTI	ATGAA	9 cut(s)	578, 710, 963, 1150, 1247, 1453, 1516, 1552, 1707
TspGWI	ACGGA	6 cut(s)	166, 640, 1205, 1299, 1417, 1581
TspMI	CCCGGG	1 cut(s)	1547
TspRI	CASTG	5 cut(s)	118, 1203, 1423, 1450, 1852
Vha464I	CTTAAG	1 cut(s)	691
VneI	GTGCAC	2 cut(s)	1183, 1446
VpaK11BI	GGWCC	1 cut(s)	107
XapI	RAATTY	1 cut(s)	1423
XceI	RCATGY	1 cut(s)	541
XmaI	CCCGGG	1 cut(s)	1547
XmiI	GTMKAC	1 cut(s)	453
XmnI	GAANNNNTTC	6 cut(s)	617, 762, 954, 1028, 1602, 1680
XspI	CTAG	2 cut(s)	1085, 1091
ZraI	GACGTC	2 cut(s)	64, 457

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.