Rw2G023400

ABC transporter G family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0015392
Physical Location & Seq
Forward (+)
34113710 .. 34116199
2490 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G023400.1

Sequence Viewer

Length: 1917 bp
ATGAAGAAACAAGGCAGTGAGATTGAGGCAGTAGGCATTAGCTACAACATTAAAATTCATCAAAGCAGAGATCTTGACTGCCCCTTAAAGATCTTCAGCACCAAAAACCATCCAGAACCAGATCAAAAGGTTCCAAAACTCGAAGAAGAAGAACCGAGCACTCCCGGAGTCCGGCGAGTGCTAAAGAACGTCAACTGCCGCGCAAAACCATGGGAAATCCTAGCCATTGTCGGTCCGAGTGGAGCCGGAAAGTCATCCCTACTTGAAATCCTGGCCGGAAAGGTGAGGCCACAAAATGGTTCAGTTTTCGTCAACCGCAAGCCGGTGGATAAAGCCAGCTTCAAGAAAGTTTCCGGGTACGTAACTCAAAAGGACACTCTCTTTCCTTTACTTACAGTTGAAGAAACCCTAATGTTCAGCGCCAAGTTGAGGCTGAGGATTCCTCAGTGTCAGCTGCGCTCCCGGGTTGAGGCACTGATCGGGGAGCTTGGCCTCACTCACGTAGCCGGAGCCAGAGTCGGCGACGACAGAGTACGTGGGATATCCGGAGGAGAAAGACGCCGTGTTTCGATCGGAGTGGACGTGATTCATGACCCGGAAGTACTGATCCTTGATGAACCTACTTCCGGGCTGGACAGCACTTCGGCTCTTCAGATCATTGACATGCTCAAAACCATGGCGGAAATCCGAGGCAGAACCATCATCCTCAGCATTCATCAACCCGGTTTTAGAATCGTGAAGCTCTTTAATTCTATTCTGTTACTGGCTAACGGCTCTGTCCTCCACCATGGAACAGTAGATCACCTTGCTCTTAACCTGAAGCTCATGGGACTAGAGCTTCCTCTCCATGTAAACATCGTCGAGTTCGCAATCGATTCCATCGAAGCCATTCATCAGCAAGAGAGTTTGCAGCACGAGCTCATTGATAATGGCAGTGGCGACTGTACCAAGAGCAGTAAGTGCACTCTTCAACAGCTGTTTCAACAGTCAAAGGTCATCGATCACGAAGAAACCGTCACCAAATCGCTCCATTCCCCACTCAATTTCGCCAATTCAAGACTTCGAGAGACGATCATCCTCACGCACAGGTTCTCGAAAAACATTTTCCGGACCAAGGAGCTGTTTGCTTGCCGGACAATTCAAATGCTGGTCTCGGGATTAGTCCTTGGTTCGATCTTCTATGATCTGAAGGATGATTTGACCGGCGCAGAAGAAAGGGTAGGTCTGTTTGCATTCATTTTAACTTTTTTACTCTCCTGCACTACAGAAGCTTTACCAATATTTCTTCAAGAAAGAGAGATTCTGATGAAGGAAACTTCTTGTGGAAGTTACCGAGTCTCATCCTACGCCATTGCCAATGGTATAGTCTACTTGCCCTTTCTTCTAATCCTAGCCATTCTCTTCACAACGCCACTGTACTGGCTCGTCGGACTCAACCCGACTTTCATGGCCTTTTGCCACTTCCTGCTTCTAATCTGGTTAATTCTCTACACCGCAAATTCCGTGGTGGTGTGTATCAGTGCTCTGGTGCCTAATTTCATAGTTGGAAACTCGGTCATCGCTGGGGTTATGGGATCGTTCTTTTTGTTTTCGGGCTACTTCATTTCGAAGCACGGTATTCCAAGTTACTGGATCTTCATGCATTACATTTCGCTGTTCAAATATCCGTTCGAGGGGTTTCTGATAAACGAGTTTTCTAATTCGGACAATTGCTTGGAGTACTTCTTTGGGAAGTGCATGGTGACCGGTGAAGACGTTCTGAGAGACGAAGGGTATGGAGAAGAGAGCCGGTGGAGGAATGTGGCGGTGATGGTGTGCTTTGTGCTTGTTTACAGGTTCATTTCTTATGTCATTCTTAGGTGTAGATGCTCCCAAAGGGGGTTCAGTTTCATAGCTGCTCGATCTTCTTTGGTTTAA

Protein Analysis

638

Amino Acids

71.55

Weight (kDa)

8.26

Isoelectric Point (pI)

43.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_tran PF00005 61 - 209 4.2e-31 ABC transporter
ABC2_membrane PF01061 357 - 565 1.2e-50 ABC-2 type transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015392)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G13610
fragaria_vesca FvH4_1g24520
malus_domestica MD15G1134300.v1.1
prunus_persica Prupe.4G272400_v2.0.a1
pyrus_communis pycom15g12090
rosa_chinensis RchiOBHm_Chr2g0121921
rosa_laevigata RLG00000018633
rosa_multiflora Rmu_co8491703.1_g000001 Rmu_sc0003880.1_g000031
rosa_roxburghii Rroxscaffold_2G00122210
rosa_rugosa Rorug02G0234800
rosa_samantha Rh2AG291600 Rh2BG302100 Rh2CG280900 Rh2DG316100
rosa_wichuraiana Rw2G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1528
AccB7I CCANNNNNTGG 1 cut(s) 296
AccI GTMKAC 1 cut(s) 1368
AccII CGCG 1 cut(s) 201
AccIII TCCGGA 2 cut(s) 545, 1107
AciI CCGC 5 cut(s) 199, 316, 680, 1494, 1805
AclWI GGATC 3 cut(s) 601, 1582, 1640
AcoI YGGCCR 1 cut(s) 273
AcsI RAATTY 2 cut(s) 54, 1498
AcuI CTGAAG 4 cut(s) 79, 635, 839, 1208
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 6 cut(s) 359, 534, 603, 946, 1418, 1721
AfiI CCNNNNNNNGG 9 cut(s) 171, 296, 322, 429, 469, 626, 1114, 1673, 1878
AgeI ACCGGT 1 cut(s) 1745
AgsI TTSAA 9 cut(s) 266, 343, 401, 971, 983, 1056, 1142, 1289, 1660
AjiI CACGTC 1 cut(s) 583
AjnI CCWGG 1 cut(s) 270
Alw21I GWGCWC 4 cut(s) 161, 921, 965, 1525
Alw26I GTCTC 4 cut(s) 1061, 1156, 1342, 1758
Alw44I GTGCAC 1 cut(s) 961
AlwI GGATC 3 cut(s) 601, 1582, 1640
Ama87I CYCGRG 2 cut(s) 462, 1153
Aor13HI TCCGGA 2 cut(s) 545, 1107
AoxI GGCC 4 cut(s) 273, 287, 490, 1449
ApaLI GTGCAC 1 cut(s) 961
ApeKI GCWGC 3 cut(s) 454, 910, 1895
ApoI RAATTY 2 cut(s) 54, 1498
ArsI GACNNNNNNTTYG 2 cut(s) 999, 1031
AsiGI ACCGGT 1 cut(s) 1745
Asp700I GAANNNNTTC 2 cut(s) 888, 1755
AspLEI GCGC 4 cut(s) 203, 422, 459, 1208
AspS9I GGNCC 2 cut(s) 233, 1110
AsuC2I CCSGG 7 cut(s) 165, 355, 463, 464, 596, 628, 723
AsuHPI GGTGA 6 cut(s) 295, 794, 1009, 1753, 1760, 1819
AsuII TTCGAA 1 cut(s) 1607
AvaI CYCGRG 2 cut(s) 462, 1153
AvaII GGWCC 2 cut(s) 233, 1110
BaeGI GKGCMC 1 cut(s) 965
BanI GGYRCC 1 cut(s) 1528
BanII GRGCYC 1 cut(s) 921
BauI CACGAG 1 cut(s) 914
BbsI GAAGAC 1 cut(s) 1758
Bbv12I GWGCWC 4 cut(s) 161, 921, 965, 1525
BbvCI CCTCAGC 2 cut(s) 434, 707
BbvI GCAGC 3 cut(s) 441, 922, 1882
BccI CCATC 4 cut(s) 117, 707, 887, 1804
BceAI ACGGC 2 cut(s) 546, 787
BciT130I CCWGG 1 cut(s) 272
BcnI CCSGG 7 cut(s) 165, 355, 463, 464, 596, 628, 723
BcoDI GTCTC 4 cut(s) 1061, 1156, 1342, 1758
BfaI CTAG 3 cut(s) 221, 833, 1391
BfmI CTRYAG 1 cut(s) 1263
BfoI RGCGCY 1 cut(s) 423
BglII AGATCT 2 cut(s) 70, 90
BisI GCNGC 4 cut(s) 199, 455, 911, 1896
BlsI GCNGC 4 cut(s) 200, 456, 912, 1897
BmcAI AGTACT 2 cut(s) 603, 1721
Bme1390I CCNGG 8 cut(s) 165, 272, 355, 463, 464, 596, 628, 723
Bme18I GGWCC 2 cut(s) 233, 1110
BmeT110I CYCGRG 2 cut(s) 462, 1153
BmgBI CACGTC 1 cut(s) 583
BmgT120I GGNCC 2 cut(s) 233, 1110
BmiI GGNNCC 4 cut(s) 132, 244, 511, 1530
BmrFI CCNGG 8 cut(s) 165, 272, 355, 463, 464, 596, 628, 723
BmsI GCATC 1 cut(s) 1856
BpiI GAAGAC 1 cut(s) 1758
BplI GAGNNNNNCTC 2 cut(s) 427, 459
Bpu10I CCTNAGC 2 cut(s) 434, 707
Bpu14I TTCGAA 1 cut(s) 1607
BpuMI CCSGG 7 cut(s) 165, 355, 463, 464, 596, 628, 723
Bsa29I ATCGAT 2 cut(s) 873, 999
BsaAI YACGTR 3 cut(s) 361, 502, 536
BsaHI GRCGYC 1 cut(s) 559
BsaI GGTCTC 1 cut(s) 1156
BsaJI CCNNGG 8 cut(s) 209, 462, 675, 688, 787, 1113, 1165, 1503
BsaWI WCCGGW 3 cut(s) 545, 1107, 1745
BsaXI ACNNNNNCTCC 4 cut(s) 501, 531, 567, 597
Bsc4I CCNNNNNNNGG 9 cut(s) 171, 296, 322, 429, 469, 626, 1114, 1673, 1878
Bse118I RCCGGY 4 cut(s) 322, 1202, 1745, 1788
Bse1I ACTGG 3 cut(s) 768, 1424, 1634
Bse3DI GCAATG 1 cut(s) 1350
BseAI TCCGGA 2 cut(s) 545, 1107
BseBI CCWGG 1 cut(s) 272
BseCI ATCGAT 2 cut(s) 873, 999
BseDI CCNNGG 8 cut(s) 209, 462, 675, 688, 787, 1113, 1165, 1503
BseGI GGATG 6 cut(s) 109, 254, 702, 1074, 1198, 1340
BseLI CCNNNNNNNGG 9 cut(s) 171, 296, 322, 429, 469, 626, 1114, 1673, 1878
BseMI GCAATG 1 cut(s) 1350
BseMII CTCAG 4 cut(s) 425, 458, 721, 1751
BseNI ACTGG 3 cut(s) 768, 1424, 1634
BseRI GAGGAG 1 cut(s) 564
BseSI GKGCMC 1 cut(s) 965
BseXI GCAGC 3 cut(s) 441, 922, 1882
BseYI CCCAGC 1 cut(s) 1562
BsgI GTGCAG 1 cut(s) 1243
Bsh1236I CGCG 1 cut(s) 201
Bsh1285I CGRYCG 1 cut(s) 573
BshFI GGCC 4 cut(s) 275, 289, 492, 1451
BshNI GGYRCC 1 cut(s) 1528
BshTI ACCGGT 1 cut(s) 1745
BshVI ATCGAT 2 cut(s) 873, 999
BsiEI CGRYCG 1 cut(s) 573
BsiHKAI GWGCWC 4 cut(s) 161, 921, 965, 1525
BsiHKCI CYCGRG 2 cut(s) 462, 1153
BslFI GGGAC 1 cut(s) 843
BslI CCNNNNNNNGG 9 cut(s) 171, 296, 322, 429, 469, 626, 1114, 1673, 1878
BsmAI GTCTC 4 cut(s) 1061, 1156, 1342, 1758
BsmBI CGTCTC 2 cut(s) 1061, 1758
BsmFI GGGAC 1 cut(s) 843
BsmI GAATGC 2 cut(s) 711, 1232
BsnI GGCC 4 cut(s) 275, 289, 492, 1451
Bso31I GGTCTC 1 cut(s) 1156
BsoBI CYCGRG 2 cut(s) 462, 1153
Bsp119I TTCGAA 1 cut(s) 1607
Bsp1286I GDGCHC 4 cut(s) 161, 921, 965, 1525
Bsp13I TCCGGA 2 cut(s) 545, 1107
Bsp19I CCATGG 3 cut(s) 209, 675, 787
BspACI CCGC 5 cut(s) 199, 316, 680, 1494, 1805
BspANI GGCC 4 cut(s) 275, 289, 492, 1451
BspCNI CTCAG 4 cut(s) 426, 457, 720, 1752
BspDI ATCGAT 2 cut(s) 873, 999
BspEI TCCGGA 2 cut(s) 545, 1107
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 589
BspLI GGNNCC 4 cut(s) 132, 244, 511, 1530
BspPI GGATC 3 cut(s) 601, 1582, 1640
BspQI GCTCTTC 1 cut(s) 654
BspT104I TTCGAA 1 cut(s) 1607
BspT107I GGYRCC 1 cut(s) 1528
BspTNI GGTCTC 1 cut(s) 1156
BsrDI GCAATG 1 cut(s) 1350
BsrFI RCCGGY 4 cut(s) 322, 1202, 1745, 1788
BsrI ACTGG 3 cut(s) 768, 1424, 1634
BssAI RCCGGY 4 cut(s) 322, 1202, 1745, 1788
BssECI CCNNGG 8 cut(s) 209, 462, 675, 688, 787, 1113, 1165, 1503
BssNI GRCGYC 1 cut(s) 559
BssSI CACGAG 1 cut(s) 914
BssT1I CCWWGG 5 cut(s) 209, 675, 787, 1113, 1165
Bst2BI CACGAG 1 cut(s) 914
Bst2UI CCWGG 1 cut(s) 272
Bst4CI ACNGT 7 cut(s) 397, 796, 944, 987, 1015, 1416, 1616
Bst6I CTCTTC 4 cut(s) 654, 972, 1406, 1776
BstACI GRCGYC 1 cut(s) 559
BstAPI GCANNNNNTGC 1 cut(s) 960
BstBAI YACGTR 3 cut(s) 361, 502, 536
BstBI TTCGAA 1 cut(s) 1607
BstC8I GCNNGC 3 cut(s) 320, 337, 1129
BstDEI CTNAG 5 cut(s) 434, 444, 707, 1760, 1856
BstDSI CCRYGG 4 cut(s) 209, 675, 787, 1503
BstEII GGTNACC 1 cut(s) 1741
BstF5I GGATG 6 cut(s) 109, 254, 702, 1074, 1198, 1340
BstFNI CGCG 1 cut(s) 201
BstH2I RGCGCY 1 cut(s) 423
BstHHI GCGC 4 cut(s) 203, 422, 459, 1208
BstMAI GTCTC 4 cut(s) 1061, 1156, 1342, 1758
BstMCI CGRYCG 1 cut(s) 573
BstMWI GCNNNNNNNGC 2 cut(s) 916, 960
BstNI CCWGG 1 cut(s) 272
BstNSI RCATGY 1 cut(s) 667
BstPI GGTNACC 1 cut(s) 1741
BstSCI CCNGG 8 cut(s) 163, 270, 353, 461, 462, 594, 626, 721
BstSFI CTRYAG 1 cut(s) 1263
BstSLI GKGCMC 1 cut(s) 965
BstSNI TACGTA 1 cut(s) 361
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 3 cut(s) 441, 922, 1882
BstV2I GAAGAC 1 cut(s) 1758
BstX2I RGATCY 3 cut(s) 70, 90, 1632
BstXI CCANNNNNNTGG 2 cut(s) 1419, 1629
BstYI RGATCY 3 cut(s) 70, 90, 1632
Bsu15I ATCGAT 2 cut(s) 873, 999
BsuRI GGCC 4 cut(s) 275, 289, 492, 1451
BsuTUI ATCGAT 2 cut(s) 873, 999
BtgI CCRYGG 4 cut(s) 209, 675, 787, 1503
BtgZI GCGATG 1 cut(s) 1543
BtrI CACGTC 1 cut(s) 583
BtsCI GGATG 6 cut(s) 109, 254, 702, 1074, 1198, 1340
BtsI GCAGTG 2 cut(s) 22, 940
BtsIMutI CAGTG 6 cut(s) 22, 452, 473, 940, 1412, 1525
Cac8I GCNNGC 3 cut(s) 320, 337, 1129
CciI TCATGA 1 cut(s) 589
CfoI GCGC 4 cut(s) 203, 422, 459, 1208
Cfr10I RCCGGY 4 cut(s) 322, 1202, 1745, 1788
Cfr13I GGNCC 2 cut(s) 233, 1110
Cfr9I CCCGGG 1 cut(s) 462
ClaI ATCGAT 2 cut(s) 873, 999
CpoI CGGWCCG 1 cut(s) 233
CseI GACGC 1 cut(s) 567
Csp6I GTAC 6 cut(s) 358, 533, 602, 945, 1417, 1720
CspAI ACCGGT 1 cut(s) 1745
CspCI CAANNNNNGTGG 2 cut(s) 1485, 1520
CspI CGGWCCG 1 cut(s) 233
CviQI GTAC 6 cut(s) 358, 533, 602, 945, 1417, 1720
DdeI CTNAG 5 cut(s) 434, 444, 707, 1760, 1856
EaeI YGGCCR 1 cut(s) 273
Eam1104I CTCTTC 4 cut(s) 654, 972, 1406, 1776
EarI CTCTTC 4 cut(s) 654, 972, 1406, 1776
EciI GGCGGA 1 cut(s) 695
Ecl136II GAGCTC 1 cut(s) 919
Eco105I TACGTA 1 cut(s) 361
Eco130I CCWWGG 5 cut(s) 209, 675, 787, 1113, 1165
Eco24I GRGCYC 1 cut(s) 921
Eco31I GGTCTC 1 cut(s) 1156
Eco32I GATATC 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 233, 1110
Eco53kI GAGCTC 1 cut(s) 919
Eco57I CTGAAG 4 cut(s) 79, 635, 839, 1208
Eco88I CYCGRG 2 cut(s) 462, 1153
Eco91I GGTNACC 1 cut(s) 1741
EcoICRI GAGCTC 1 cut(s) 919
EcoO65I GGTNACC 1 cut(s) 1741
EcoRII CCWGG 1 cut(s) 270
EcoRV GATATC 1 cut(s) 543
EcoT14I CCWWGG 5 cut(s) 209, 675, 787, 1113, 1165
EcoT22I ATGCAT 1 cut(s) 1644
EcoT38I GRGCYC 1 cut(s) 921
ErhI CCWWGG 5 cut(s) 209, 675, 787, 1113, 1165
Esp3I CGTCTC 2 cut(s) 1061, 1758
FaqI GGGAC 1 cut(s) 843
FblI GTMKAC 1 cut(s) 1368
Fnu4HI GCNGC 4 cut(s) 199, 455, 911, 1896
FokI GGATG 6 cut(s) 96, 241, 689, 1061, 1205, 1327
FriOI GRGCYC 1 cut(s) 921
Fsp4HI GCNGC 4 cut(s) 199, 455, 911, 1896
FspBI CTAG 3 cut(s) 221, 833, 1391
GlaI GCGC 4 cut(s) 202, 421, 458, 1207
GluI GCNGC 4 cut(s) 199, 455, 911, 1896
GsaI CCCAGC 1 cut(s) 1566
HaeII RGCGCY 1 cut(s) 423
HaeIII GGCC 4 cut(s) 275, 289, 492, 1451
HgaI GACGC 1 cut(s) 567
HhaI GCGC 4 cut(s) 203, 422, 459, 1208
Hin1I GRCGYC 1 cut(s) 559
Hin6I GCGC 4 cut(s) 201, 420, 457, 1206
HinP1I GCGC 4 cut(s) 201, 420, 457, 1206
HincII GTYRAC 2 cut(s) 193, 313
HindII GTYRAC 2 cut(s) 193, 313
HindIII AAGCTT 1 cut(s) 1269
HinfI GANTC 9 cut(s) 168, 439, 516, 586, 732, 875, 1300, 1335, 1431
HphI GGTGA 6 cut(s) 295, 794, 1009, 1753, 1760, 1819
Hpy166II GTNNAC 7 cut(s) 193, 313, 580, 853, 963, 1369, 1831
Hpy8I GTNNAC 7 cut(s) 193, 313, 580, 853, 963, 1369, 1831
Hpy99I CGWCG 3 cut(s) 527, 863, 1430
HpyAV CCTTC 3 cut(s) 1183, 1303, 1763
HpyCH4III ACNGT 7 cut(s) 397, 796, 944, 987, 1015, 1416, 1616
HpyCH4IV ACGT 6 cut(s) 189, 360, 501, 535, 582, 1755
HpyCH4V TGCA 6 cut(s) 910, 963, 1232, 1260, 1642, 1737
HpyF10VI GCNNNNNNNGC 2 cut(s) 916, 960
HpyF3I CTNAG 5 cut(s) 434, 444, 707, 1760, 1856
HpySE526I ACGT 6 cut(s) 189, 360, 501, 535, 582, 1755
Hsp92I GRCGYC 1 cut(s) 559
HspAI GCGC 4 cut(s) 201, 420, 457, 1206
Kpn2I TCCGGA 2 cut(s) 545, 1107
LguI GCTCTTC 1 cut(s) 654
LmnI GCTCC 7 cut(s) 242, 464, 484, 509, 1032, 1117, 1874
Lsp1109I GCAGC 3 cut(s) 441, 922, 1882
LweI GCATC 1 cut(s) 1856
MaeI CTAG 3 cut(s) 221, 833, 1391
MaeII ACGT 6 cut(s) 189, 360, 501, 535, 582, 1755
MaeIII GTNAC 6 cut(s) 361, 759, 1015, 1328, 1625, 1741
MfeI CAATTG 1 cut(s) 1708
MflI RGATCY 3 cut(s) 70, 90, 1632
MhlI GDGCHC 4 cut(s) 161, 921, 965, 1525
MlyI GAGTC 4 cut(s) 177, 525, 1344, 1425
MmeI TCCRAC 2 cut(s) 1408, 1525
Mph1103I ATGCAT 1 cut(s) 1644
MroI TCCGGA 2 cut(s) 545, 1107
MroXI GAANNNNTTC 2 cut(s) 888, 1755
MseI TTAA 7 cut(s) 51, 86, 747, 813, 1241, 1481, 1915
MslI CAYNNNNRTG 1 cut(s) 662
MspA1I CMGCKG 2 cut(s) 454, 976
MspR9I CCNGG 8 cut(s) 165, 272, 355, 463, 464, 596, 628, 723
MunI CAATTG 1 cut(s) 1708
Mva1269I GAATGC 2 cut(s) 711, 1232
MvaI CCWGG 1 cut(s) 272
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 2 cut(s) 916, 960
NciI CCSGG 7 cut(s) 165, 355, 463, 464, 596, 628, 723
NcoI CCATGG 3 cut(s) 209, 675, 787
NlaIV GGNNCC 4 cut(s) 132, 244, 511, 1530
NmuCI GTSAC 2 cut(s) 1015, 1741
NsiI ATGCAT 1 cut(s) 1644
NspI RCATGY 1 cut(s) 667
NspV TTCGAA 1 cut(s) 1607
PagI TCATGA 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 654
PcsI WCGNNNNNNNCGW 3 cut(s) 579, 879, 1011
PctI GAATGC 2 cut(s) 711, 1232
PdmI GAANNNNTTC 2 cut(s) 888, 1755
PfeI GAWTC 5 cut(s) 439, 586, 732, 875, 1300
PflMI CCANNNNNTGG 1 cut(s) 296
PfoI TCCNGGA 1 cut(s) 163
PinAI ACCGGT 1 cut(s) 1745
PkrI GCNGC 4 cut(s) 200, 456, 912, 1897
Ple19I CGATCG 1 cut(s) 573
PleI GAGTC 4 cut(s) 176, 524, 1343, 1425
PpsI GAGTC 4 cut(s) 176, 524, 1343, 1425
Ppu21I YACGTR 3 cut(s) 361, 502, 536
Psp124BI GAGCTC 1 cut(s) 921
Psp6I CCWGG 1 cut(s) 270
PspEI GGTNACC 1 cut(s) 1741
PspFI CCCAGC 1 cut(s) 1562
PspGI CCWGG 1 cut(s) 270
PspN4I GGNNCC 4 cut(s) 132, 244, 511, 1530
PspPI GGNCC 2 cut(s) 233, 1110
PsuI RGATCY 3 cut(s) 70, 90, 1632
PvuI CGATCG 1 cut(s) 573
PvuII CAGCTG 2 cut(s) 454, 976
RsaI GTAC 6 cut(s) 359, 534, 603, 946, 1418, 1721
RsaNI GTAC 6 cut(s) 358, 533, 602, 945, 1417, 1720
RseI CAYNNNNRTG 1 cut(s) 662
Rsr2I CGGWCCG 1 cut(s) 233
RsrII CGGWCCG 1 cut(s) 233
SacI GAGCTC 1 cut(s) 921
SapI GCTCTTC 1 cut(s) 654
SaqAI TTAA 7 cut(s) 51, 86, 747, 813, 1241, 1481, 1915
SatI GCNGC 4 cut(s) 199, 455, 911, 1896
Sau96I GGNCC 2 cut(s) 233, 1110
ScaI AGTACT 2 cut(s) 603, 1721
SchI GAGTC 4 cut(s) 177, 525, 1344, 1425
ScrFI CCNGG 8 cut(s) 165, 272, 355, 463, 464, 596, 628, 723
SduI GDGCHC 4 cut(s) 161, 921, 965, 1525
SfaNI GCATC 1 cut(s) 1856
SfcI CTRYAG 1 cut(s) 1263
SfuI TTCGAA 1 cut(s) 1607
SinI GGWCC 2 cut(s) 233, 1110
SmaI CCCGGG 1 cut(s) 464
SmiMI CAYNNNNRTG 1 cut(s) 662
SnaBI TACGTA 1 cut(s) 361
SsiI CCGC 5 cut(s) 199, 316, 680, 1494, 1805
SspI AATATT 1 cut(s) 1281
SspMI CTAG 3 cut(s) 221, 833, 1391
SstI GAGCTC 1 cut(s) 921
StyD4I CCNGG 8 cut(s) 163, 270, 353, 461, 462, 594, 626, 721
StyI CCWWGG 5 cut(s) 209, 675, 787, 1113, 1165
TaaI ACNGT 7 cut(s) 397, 796, 944, 987, 1015, 1416, 1616
TaiI ACGT 6 cut(s) 192, 363, 504, 538, 585, 1758
TaqII GACCGA 2 cut(s) 221, 1543
TatI WGTACW 3 cut(s) 601, 1416, 1719
TauI GCSGC 1 cut(s) 201
TfiI GAWTC 5 cut(s) 439, 586, 732, 875, 1300
Tru1I TTAA 7 cut(s) 51, 86, 747, 813, 1241, 1481, 1915
Tru9I TTAA 7 cut(s) 51, 86, 747, 813, 1241, 1481, 1915
TscAI CASTG 6 cut(s) 22, 452, 480, 940, 1419, 1525
TseFI GTSAC 2 cut(s) 1015, 1741
TseI GCWGC 3 cut(s) 454, 910, 1895
Tsp45I GTSAC 2 cut(s) 1015, 1741
TspGWI ACGGA 2 cut(s) 1492, 1656
TspMI CCCGGG 1 cut(s) 462
TspRI CASTG 6 cut(s) 22, 452, 480, 940, 1419, 1525
Van91I CCANNNNNTGG 1 cut(s) 296
VneI GTGCAC 1 cut(s) 961
VpaK11BI GGWCC 2 cut(s) 233, 1110
XapI RAATTY 2 cut(s) 54, 1498
XceI RCATGY 1 cut(s) 667
XmaI CCCGGG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 1368
XmnI GAANNNNTTC 2 cut(s) 888, 1755
XspI CTAG 3 cut(s) 221, 833, 1391
ZrmI AGTACT 2 cut(s) 603, 1721
Zsp2I ATGCAT 1 cut(s) 1644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.