pycom15g15890

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016060
Physical Location & Seq
Reverse (-)
11077458 .. 11079012
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
ATGGAGAATGTCAAGTTGTTGATACCGGTGATGGCAACAAGTATAGTAGACGTGATGGACAAATGGTCAGCAATGTCCAGCTCCGGAGAGGTAGAGATTGAAGTTTCCGAGTGGTTCCAAAACCTAACTGAAGACATAATTACCCGTACGGCGTTCGGAAGCAGCTACGAAGATGGAAAGGCCGTTTTCCGGCTACAAGCTCAGCAAATGGCGTTTGTTACCGATGCTTTTCAAAAAGTTTGCATCCCCGGTTACAGATTCCTGCCCACAAGAAGGAACATAAATTCTTGGAAATTGGACAAGCAAATTAGGAAGTCGTTGATAAAGCTGATCGATCGACGGCTAGAGAATTCGAACTGGGGCGACAATGTGCATGATAAATGTCCAAAAGATTTGCTAGGTCTTATGATTCAGGCCTCAAATTCTTCCCCTTCTTCCAACATCACTGTCAATGACATTGTTGAAGAATGCAAGAGCTTCTTCTTTGCGGGAAAACACACCACATCCAATTTGCTGACATGGGCAACGGTTCTCCTAGCAATGCACCCACAGTGGCAGCTACAGGCACGTGACGAGGTGCTGAGGGTGTGTGGATCACGTGACATACCCACAAAAGATGATGTCGTAAAGCTTAAGACGCTGAATATGATCCTCAATGAATCACTACGATTGTACCCACCTGTGATCGCGACGATCCGGCGGTCGAGAACAGATGTGGAGCTCGGGGGATACAAGGTCCCACGTGGGACCGAGCTTCTGATTCCGATCATAGCCGTCCATCATGATCAGAGCATATGGGGCAATGATGCAAAGGAGTTCAACCCGGGACGGTTCGCGGAGGGTGTGGCGCGGGCGGCCAAGCACCCGGTGGCATTCATTCCATTCGGGCTTGGGGTCCGCACATGCATCGGGCAGAATCTAGCGCTGTTGCAGGCAAAACTAGCCCTTGCCATCATGCTTCAACGCTTCTCTTTCAGGCTGGCCCCCACCTATCAACATGCACCTACGGTACTGATGCTACTTCACCCACAATATGGTGTACCCATCATTTTCCAACGCCTGCCACAACATGATCAGGGTCAAGATCAAGATCAAGATCGAAATCAAGGGCCATGA

Protein Analysis

372

Amino Acids

41.9

Weight (kDa)

8.73

Isoelectric Point (pI)

41.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 7 - 333 1.1e-68 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26710
fragaria_vesca FvH4_1g03910
malus_domestica MD02G1037300.v1.1 MD15G1177600.v1.1
prunus_persica Prupe.7G237900_v2.0.a1
pyrus_communis pycom02g03060 pycom15g15890
rosa_chinensis RchiOBHm_Chr2g0089381
rosa_laevigata RLG00000016022
rosa_multiflora Rmu_sc0000197.1_g000024
rosa_roxburghii Rroxscaffold_2G00152010
rosa_rugosa Rorug01G0490300
rosa_samantha Rh2AG045400 Rh2DG045300
rosa_wichuraiana Rw2G003730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1034
AccI GTMKAC 1 cut(s) 48
AccII CGCG 3 cut(s) 689, 836, 850
AccIII TCCGGA 1 cut(s) 83
AciI CCGC 6 cut(s) 488, 700, 836, 850, 854, 898
AclWI GGATC 3 cut(s) 601, 643, 688
AcoI YGGCCR 1 cut(s) 855
AcsI RAATTY 3 cut(s) 283, 349, 421
AcuI CTGAAG 1 cut(s) 150
AcvI CACGTG 3 cut(s) 569, 599, 743
AdeI CACNNNGTG 1 cut(s) 868
AfaI GTAC 4 cut(s) 148, 674, 1011, 1041
AfeI AGCGCT 1 cut(s) 924
AfiI CCNNNNNNNGG 3 cut(s) 189, 273, 1034
AflII CTTAAG 1 cut(s) 632
AgeI ACCGGT 1 cut(s) 25
AgsI TTSAA 5 cut(s) 101, 233, 464, 820, 962
AhdI GACNNNNNGTC 1 cut(s) 64
AjiI CACGTC 1 cut(s) 52
AluBI AGCT 9 cut(s) 81, 165, 200, 328, 477, 559, 631, 721, 754
AluI AGCT 9 cut(s) 81, 165, 200, 328, 477, 559, 631, 721, 754
Alw21I GWGCWC 1 cut(s) 723
AlwI GGATC 3 cut(s) 601, 643, 688
Ama87I CYCGRG 2 cut(s) 722, 823
Aor13HI TCCGGA 1 cut(s) 83
Aor51HI AGCGCT 1 cut(s) 924
AoxI GGCC 5 cut(s) 180, 414, 855, 981, 1109
ApeKI GCWGC 2 cut(s) 162, 556
ApoI RAATTY 3 cut(s) 283, 349, 421
AsiGI ACCGGT 1 cut(s) 25
AspLEI GCGC 2 cut(s) 850, 925
AspS9I GGNCC 5 cut(s) 736, 747, 895, 982, 1109
AsuC2I CCSGG 4 cut(s) 249, 824, 825, 866
AsuHPI GGTGA 2 cut(s) 40, 1016
AsuII TTCGAA 1 cut(s) 353
AvaI CYCGRG 2 cut(s) 722, 823
AvaII GGWCC 3 cut(s) 736, 747, 895
BaeI ACNNNNGTAYC 2 cut(s) 656, 689
BanII GRGCYC 1 cut(s) 723
BbrPI CACGTG 3 cut(s) 569, 599, 743
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 723
BbvCI CCTCAGC 1 cut(s) 581
BbvI GCAGC 2 cut(s) 174, 568
BccI CCATC 6 cut(s) 25, 49, 167, 786, 959, 1052
BceAI ACGGC 4 cut(s) 165, 167, 356, 758
BcgI CGANNNNNNTGC 2 cut(s) 889, 923
BciVI GTATCC 1 cut(s) 722
BclI TGATCA 2 cut(s) 784, 1072
BcnI CCSGG 4 cut(s) 249, 824, 825, 866
BfaI CTAG 5 cut(s) 344, 398, 536, 920, 941
BfmI CTRYAG 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 926
BfrI CTTAAG 1 cut(s) 632
BfuI GTATCC 1 cut(s) 722
BisI GCNGC 3 cut(s) 163, 557, 855
BlpI GCTNAGC 1 cut(s) 201
BlsI GCNGC 3 cut(s) 164, 558, 856
Bme1390I CCNGG 4 cut(s) 249, 824, 825, 866
Bme18I GGWCC 3 cut(s) 736, 747, 895
BmeRI GACNNNNNGTC 1 cut(s) 64
BmeT110I CYCGRG 2 cut(s) 722, 823
BmgBI CACGTC 1 cut(s) 52
BmgT120I GGNCC 5 cut(s) 736, 747, 895, 982, 1109
BmiI GGNNCC 5 cut(s) 116, 738, 748, 896, 984
BmrFI CCNGG 4 cut(s) 249, 824, 825, 866
BmrI ACTGGG 1 cut(s) 367
BmsI GCATC 5 cut(s) 214, 252, 796, 915, 1005
BmuI ACTGGG 1 cut(s) 367
BpiI GAAGAC 1 cut(s) 138
Bpu10I CCTNAGC 1 cut(s) 581
Bpu1102I GCTNAGC 1 cut(s) 201
Bpu14I TTCGAA 1 cut(s) 353
BpuMI CCSGG 4 cut(s) 249, 824, 825, 866
Bsa29I ATCGAT 1 cut(s) 333
BsaAI YACGTR 3 cut(s) 569, 599, 743
BsaBI GATNNNNATC 4 cut(s) 764, 1089, 1095, 1101
BsaJI CCNNGG 2 cut(s) 247, 823
BsaWI WCCGGW 2 cut(s) 25, 83
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 273, 1034
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 3 cut(s) 78, 546, 808
Bse8I GATNNNNATC 4 cut(s) 764, 1089, 1095, 1101
BseAI TCCGGA 1 cut(s) 83
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 247, 823
BseGI GGATG 2 cut(s) 243, 503
BseJI GATNNNNATC 4 cut(s) 764, 1089, 1095, 1101
BseLI CCNNNNNNNGG 3 cut(s) 189, 273, 1034
BseMI GCAATG 3 cut(s) 78, 546, 808
BseMII CTCAG 2 cut(s) 215, 572
BseNI ACTGG 1 cut(s) 362
BseXI GCAGC 2 cut(s) 174, 568
Bsh1236I CGCG 3 cut(s) 689, 836, 850
Bsh1285I CGRYCG 2 cut(s) 337, 704
BshFI GGCC 5 cut(s) 182, 416, 857, 983, 1111
BshTI ACCGGT 1 cut(s) 25
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 2 cut(s) 337, 704
BsiHKAI GWGCWC 1 cut(s) 723
BsiHKCI CYCGRG 2 cut(s) 722, 823
BsiSI CCGG 7 cut(s) 26, 84, 190, 249, 697, 824, 866
BsiWI CGTACG 1 cut(s) 146
BslFI GGGAC 3 cut(s) 722, 760, 840
BslI CCNNNNNNNGG 3 cut(s) 189, 273, 1034
BsmFI GGGAC 3 cut(s) 722, 760, 840
BsmI GAATGC 2 cut(s) 473, 872
BsnI GGCC 5 cut(s) 182, 416, 857, 983, 1111
BsoBI CYCGRG 2 cut(s) 722, 823
Bsp119I TTCGAA 1 cut(s) 353
Bsp1286I GDGCHC 1 cut(s) 723
Bsp13I TCCGGA 1 cut(s) 83
Bsp1720I GCTNAGC 1 cut(s) 201
Bsp68I TCGCGA 1 cut(s) 689
BspACI CCGC 6 cut(s) 488, 700, 836, 850, 854, 898
BspANI GGCC 5 cut(s) 182, 416, 857, 983, 1111
BspCNI CTCAG 2 cut(s) 214, 573
BspDI ATCGAT 1 cut(s) 333
BspEI TCCGGA 1 cut(s) 83
BspFNI CGCG 3 cut(s) 689, 836, 850
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 5 cut(s) 116, 738, 748, 896, 984
BspPI GGATC 3 cut(s) 601, 643, 688
BspT104I TTCGAA 1 cut(s) 353
BspTI CTTAAG 1 cut(s) 632
BsrDI GCAATG 3 cut(s) 78, 546, 808
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 1 cut(s) 362
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 2 cut(s) 247, 823
Bst4CI ACNGT 5 cut(s) 448, 529, 552, 831, 1009
BstAFI CTTAAG 1 cut(s) 632
BstBAI YACGTR 3 cut(s) 569, 599, 743
BstBI TTCGAA 1 cut(s) 353
BstC8I GCNNGC 4 cut(s) 852, 933, 981, 1061
BstDEI CTNAG 2 cut(s) 201, 581
BstF5I GGATG 2 cut(s) 243, 503
BstFNI CGCG 3 cut(s) 689, 836, 850
BstH2I RGCGCY 1 cut(s) 926
BstHHI GCGC 2 cut(s) 850, 925
BstMCI CGRYCG 2 cut(s) 337, 704
BstMWI GCNNNNNNNGC 4 cut(s) 637, 798, 854, 941
BstNSI RCATGY 2 cut(s) 906, 1001
BstSCI CCNGG 4 cut(s) 247, 822, 823, 864
BstSFI CTRYAG 1 cut(s) 560
BstUI CGCG 3 cut(s) 689, 836, 850
BstV1I GCAGC 2 cut(s) 174, 568
BstV2I GAAGAC 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 333
BsuI GTATCC 1 cut(s) 722
BsuRI GGCC 5 cut(s) 182, 416, 857, 983, 1111
BsuTUI ATCGAT 1 cut(s) 333
BtrI CACGTC 1 cut(s) 52
BtsCI GGATG 2 cut(s) 243, 503
BtsIMutI CAGTG 2 cut(s) 444, 557
BtuMI TCGCGA 1 cut(s) 689
Cac8I GCNNGC 4 cut(s) 852, 933, 981, 1061
CciI TCATGA 1 cut(s) 781
CfoI GCGC 2 cut(s) 850, 925
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 5 cut(s) 736, 747, 895, 982, 1109
Cfr9I CCCGGG 1 cut(s) 823
ClaI ATCGAT 1 cut(s) 333
CseI GACGC 1 cut(s) 646
Csp6I GTAC 4 cut(s) 147, 673, 1010, 1040
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 8 cut(s) 374, 519, 782, 903, 955, 998, 1070, 1113
CviQI GTAC 4 cut(s) 147, 673, 1010, 1040
DdeI CTNAG 2 cut(s) 201, 581
DraIII CACNNNGTG 1 cut(s) 868
DriI GACNNNNNGTC 1 cut(s) 64
EaeI YGGCCR 1 cut(s) 855
Eam1105I GACNNNNNGTC 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 721
Eco147I AGGCCT 1 cut(s) 416
Eco24I GRGCYC 1 cut(s) 723
Eco47I GGWCC 3 cut(s) 736, 747, 895
Eco47III AGCGCT 1 cut(s) 924
Eco53kI GAGCTC 1 cut(s) 721
Eco57I CTGAAG 1 cut(s) 150
Eco72I CACGTG 3 cut(s) 569, 599, 743
Eco88I CYCGRG 2 cut(s) 722, 823
EcoICRI GAGCTC 1 cut(s) 721
EcoO109I RGGNCCY 1 cut(s) 736
EcoRI GAATTC 1 cut(s) 349
EcoT22I ATGCAT 1 cut(s) 908
EcoT38I GRGCYC 1 cut(s) 723
FaeI CATG 8 cut(s) 377, 522, 785, 906, 958, 1001, 1073, 1116
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 3 cut(s) 722, 760, 840
FatI CATG 8 cut(s) 373, 518, 781, 902, 954, 997, 1069, 1112
FauI CCCGC 2 cut(s) 481, 843
FauNDI CATATG 1 cut(s) 794
FbaI TGATCA 2 cut(s) 784, 1072
FblI GTMKAC 1 cut(s) 48
Fnu4HI GCNGC 3 cut(s) 163, 557, 855
FokI GGATG 2 cut(s) 230, 490
FriOI GRGCYC 1 cut(s) 723
Fsp4HI GCNGC 3 cut(s) 163, 557, 855
FspBI CTAG 5 cut(s) 344, 398, 536, 920, 941
GlaI GCGC 2 cut(s) 849, 924
GluI GCNGC 3 cut(s) 163, 557, 855
HaeII RGCGCY 1 cut(s) 926
HaeIII GGCC 5 cut(s) 182, 416, 857, 983, 1111
HapII CCGG 7 cut(s) 26, 84, 190, 249, 697, 824, 866
HgaI GACGC 1 cut(s) 646
HhaI GCGC 2 cut(s) 850, 925
Hin1II CATG 8 cut(s) 377, 522, 785, 906, 958, 1001, 1073, 1116
Hin6I GCGC 2 cut(s) 848, 923
HinP1I GCGC 2 cut(s) 848, 923
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 5 cut(s) 258, 409, 659, 760, 916
HpaII CCGG 7 cut(s) 26, 84, 190, 249, 697, 824, 866
HphI GGTGA 2 cut(s) 40, 1016
Hpy166II GTNNAC 2 cut(s) 49, 1040
Hpy188I TCNGA 5 cut(s) 109, 158, 759, 765, 789
Hpy188III TCNNGA 7 cut(s) 84, 688, 705, 782, 1082, 1088, 1094
Hpy8I GTNNAC 2 cut(s) 49, 1040
Hpy99I CGWCG 2 cut(s) 342, 694
HpyAV CCTTC 2 cut(s) 267, 441
HpyCH4III ACNGT 5 cut(s) 448, 529, 552, 831, 1009
HpyCH4IV ACGT 4 cut(s) 51, 568, 598, 742
HpyCH4V TGCA 8 cut(s) 243, 373, 471, 544, 809, 906, 931, 1001
HpyF10VI GCNNNNNNNGC 4 cut(s) 637, 798, 854, 941
HpyF3I CTNAG 2 cut(s) 201, 581
HpySE526I ACGT 4 cut(s) 51, 568, 598, 742
Hsp92II CATG 8 cut(s) 377, 522, 785, 906, 958, 1001, 1073, 1116
HspAI GCGC 2 cut(s) 848, 923
Kpn2I TCCGGA 1 cut(s) 83
Ksp22I TGATCA 2 cut(s) 784, 1072
LmnI GCTCC 2 cut(s) 86, 718
Lsp1109I GCAGC 2 cut(s) 174, 568
LweI GCATC 5 cut(s) 214, 252, 796, 915, 1005
MaeI CTAG 5 cut(s) 344, 398, 536, 920, 941
MaeII ACGT 4 cut(s) 51, 568, 598, 742
MaeIII GTNAC 4 cut(s) 217, 251, 569, 599
MboII GAAGA 6 cut(s) 143, 182, 417, 426, 472, 476
MhlI GDGCHC 1 cut(s) 723
MluCI AATT 7 cut(s) 138, 283, 293, 306, 349, 421, 508
MmeI TCCRAC 2 cut(s) 462, 1078
MnlI CCTC 6 cut(s) 82, 427, 568, 576, 662, 832
Mph1103I ATGCAT 1 cut(s) 908
MroI TCCGGA 1 cut(s) 83
MseI TTAA 1 cut(s) 633
MspCI CTTAAG 1 cut(s) 632
MspI CCGG 7 cut(s) 26, 84, 190, 249, 697, 824, 866
MspR9I CCNGG 4 cut(s) 249, 824, 825, 866
Mva1269I GAATGC 2 cut(s) 473, 872
MvnI CGCG 3 cut(s) 689, 836, 850
MwoI GCNNNNNNNGC 4 cut(s) 637, 798, 854, 941
NciI CCSGG 4 cut(s) 249, 824, 825, 866
NdeI CATATG 1 cut(s) 794
NlaIII CATG 8 cut(s) 377, 522, 785, 906, 958, 1001, 1073, 1116
NlaIV GGNNCC 5 cut(s) 116, 738, 748, 896, 984
NmuCI GTSAC 2 cut(s) 569, 599
NruI TCGCGA 1 cut(s) 689
NsiI ATGCAT 1 cut(s) 908
NspI RCATGY 2 cut(s) 906, 1001
NspV TTCGAA 1 cut(s) 353
PagI TCATGA 1 cut(s) 781
PceI AGGCCT 1 cut(s) 416
PctI GAATGC 2 cut(s) 473, 872
PfeI GAWTC 5 cut(s) 258, 409, 659, 760, 916
Pfl23II CGTACG 1 cut(s) 146
PflMI CCANNNNNTGG 1 cut(s) 1034
PinAI ACCGGT 1 cut(s) 25
PkrI GCNGC 3 cut(s) 164, 558, 856
Ple19I CGATCG 1 cut(s) 337
PmaCI CACGTG 3 cut(s) 569, 599, 743
PmlI CACGTG 3 cut(s) 569, 599, 743
Ppu21I YACGTR 3 cut(s) 569, 599, 743
PpuMI RGGWCCY 1 cut(s) 736
Psp124BI GAGCTC 1 cut(s) 723
Psp5II RGGWCCY 1 cut(s) 736
PspCI CACGTG 3 cut(s) 569, 599, 743
PspLI CGTACG 1 cut(s) 146
PspN4I GGNNCC 5 cut(s) 116, 738, 748, 896, 984
PspPI GGNCC 5 cut(s) 736, 747, 895, 982, 1109
PspPPI RGGWCCY 1 cut(s) 736
PvuI CGATCG 1 cut(s) 337
RruI TCGCGA 1 cut(s) 689
RsaI GTAC 4 cut(s) 148, 674, 1011, 1041
RsaNI GTAC 4 cut(s) 147, 673, 1010, 1040
SacI GAGCTC 1 cut(s) 723
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 3 cut(s) 163, 557, 855
Sau96I GGNCC 5 cut(s) 736, 747, 895, 982, 1109
ScrFI CCNGG 4 cut(s) 249, 824, 825, 866
SduI GDGCHC 1 cut(s) 723
SfaNI GCATC 5 cut(s) 214, 252, 796, 915, 1005
SfcI CTRYAG 1 cut(s) 560
SfuI TTCGAA 1 cut(s) 353
SinI GGWCC 3 cut(s) 736, 747, 895
SmaI CCCGGG 1 cut(s) 825
SmlI CTYRAG 1 cut(s) 632
SmoI CTYRAG 1 cut(s) 632
Sse9I AATT 7 cut(s) 138, 283, 293, 306, 349, 421, 508
SseBI AGGCCT 1 cut(s) 416
SsiI CCGC 6 cut(s) 488, 700, 836, 850, 854, 898
SspMI CTAG 5 cut(s) 344, 398, 536, 920, 941
SstI GAGCTC 1 cut(s) 723
StuI AGGCCT 1 cut(s) 416
StyD4I CCNGG 4 cut(s) 247, 822, 823, 864
TaaI ACNGT 5 cut(s) 448, 529, 552, 831, 1009
TaiI ACGT 4 cut(s) 54, 571, 601, 745
TaqI TCGA 5 cut(s) 333, 337, 353, 704, 1099
TaqII GACCGA 1 cut(s) 764
TasI AATT 7 cut(s) 138, 283, 293, 306, 349, 421, 508
TauI GCSGC 1 cut(s) 857
TfiI GAWTC 5 cut(s) 258, 409, 659, 760, 916
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TscAI CASTG 2 cut(s) 451, 557
TseFI GTSAC 2 cut(s) 569, 599
TseI GCWGC 2 cut(s) 162, 556
Tsp45I GTSAC 2 cut(s) 569, 599
TspDTI ATGAA 2 cut(s) 672, 865
TspMI CCCGGG 1 cut(s) 823
TspRI CASTG 2 cut(s) 451, 557
Van91I CCANNNNNTGG 1 cut(s) 1034
Vha464I CTTAAG 1 cut(s) 632
VpaK11BI GGWCC 3 cut(s) 736, 747, 895
XapI RAATTY 3 cut(s) 283, 349, 421
XceI RCATGY 2 cut(s) 906, 1001
XcmI CCANNNNNNNNNTGG 1 cut(s) 865
XmaI CCCGGG 1 cut(s) 823
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 5 cut(s) 344, 398, 536, 920, 941
Zsp2I ATGCAT 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.